FastQCFastQC Report
Thu 26 May 2016
SRR1513073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488006
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC52010.2090429042373692No Hit
CCCATGTACTCTGCGTTGATACCAC42290.16997547433567284No Hit
GTCCTACAGTGGACATTTCTAAATT26700.10731485374231413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC8250.012.8992094
GGACCGT951.0419517E-611.9987066
CTGGTCG1001.934368E-611.3983129
GGACGTG10600.011.2912356
GTAGGAC16400.011.2395313
AGGACGT11050.011.1775125
CTAGGAC2750.011.0562423
GGCGAGG5350.011.008619
TGTAGGA18150.010.9938652
GTCCTAC16800.010.98541
GCGTTTA1303.2789103E-810.95925313
TGCACCG700.001490788310.8581555
GACTGCG700.001493792410.8555357
TACACCG803.7691422E-410.6884975
AGGACCT13800.010.6712845
TTAGGAC9000.010.5571773
ACCGTGT1002.4038896E-510.4484528
GTCCTAA7250.010.3660311
TAGGACG11550.010.3654364
TGTCCAC5100.010.24358110
Produced by FastQC (version 0.11.5)