FastQCFastQC Report
Thu 26 May 2016
SRR1513073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2488006
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC61510.2472260918984922No Hit
CCCATGTACTCTGCGTTGATACCAC56630.22761199128941006No Hit
GTATCAACGCAGAGTACTTTTTTTT36420.1463822836440105No Hit
GTCCTACAGTGGACATTTCTAAATT27600.11093220836284157No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTTT456.80357E-414.76651213
GTCGGTT456.80357E-414.76651212
TAGGACC7400.012.3512064
GTAGGAC15250.011.3624353
TCGAACT1201.3021054E-711.06731419
GACCGCT700.001509803210.8416687
TGGCGAG14900.010.82674218
GTCCTAC16950.010.682111
TGTAGGA17800.010.5975212
TCTACAC4800.010.5124643
GGACCTG12850.010.44665056
TTAGGAC9050.010.4149333
TCCTACA19150.010.3479362
TAGGACA6850.010.2851594
ATGGCGA16500.010.24025917
TATGGCG16250.010.16455316
GGCGAGA10650.010.15431619
CTACACT6100.010.1450314
AGGACGT12500.010.1300485
CACCGAA750.002680045310.1188917
Produced by FastQC (version 0.11.5)