FastQCFastQC Report
Thu 26 May 2016
SRR1513072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484497
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC77910.3135846008266462No Hit
CCCATGTACTCTGCGTTGATACCAC63680.25630942601258927No Hit
GTATCAACGCAGAGTACTTTTTTTT32420.1304891895623138No Hit
GAGTACATGGGAAGCAGTGGTATCA30800.12396875504377747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGCGT456.770323E-414.7760556
GTAGGAC11850.012.3477843
TAGGACC6700.011.9121774
TGTAGGA12950.011.6657862
GTCCTAC11300.011.532611
CTGTAGG13050.011.1523491
TGGCGAG10750.011.134706518
TATGGCG11100.010.78296116
GGCGAGA6900.010.73874419
GTATTAG2350.010.5242441
GCGTTTA1553.6725396E-910.41814113
TTAGGAC6400.010.3921343
GTCCTAA6050.010.3770531
TCCTACA13550.010.307772
TAGGACG7500.010.261494
ATGGCGA11800.010.22379617
CGTTTAT1606.188202E-910.09257314
TAGGACT4450.010.0351654
AGGACGT8050.010.0315025
AGGACCT10550.09.9957065
Produced by FastQC (version 0.11.5)