##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513072_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2484497
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.58590612103778	31.0	30.0	34.0	25.0	34.0
2	29.30826279927084	31.0	30.0	34.0	22.0	34.0
3	29.696073692179947	31.0	30.0	34.0	25.0	34.0
4	32.57328787275654	35.0	33.0	37.0	22.0	37.0
5	32.836103645929136	37.0	33.0	37.0	25.0	37.0
6	33.29129115470858	37.0	35.0	37.0	28.0	37.0
7	33.273836514996795	37.0	35.0	37.0	28.0	37.0
8	33.275193328871	37.0	35.0	37.0	28.0	37.0
9	34.694240323091556	39.0	35.0	39.0	27.0	39.0
10	34.19686922544081	39.0	35.0	39.0	25.0	39.0
11	34.580969910609674	39.0	35.0	39.0	27.0	39.0
12	34.41678255196122	39.0	35.0	39.0	27.0	39.0
13	34.511797760270994	39.0	35.0	39.0	27.0	39.0
14	34.852952529224225	40.0	34.0	41.0	18.0	41.0
15	35.408475437885414	40.0	35.0	41.0	26.0	41.0
16	35.012996996977655	40.0	35.0	41.0	22.0	41.0
17	34.54708619088693	40.0	34.0	41.0	17.0	41.0
18	33.95753506645409	39.0	33.0	41.0	15.0	41.0
19	34.860106089884596	40.0	34.0	41.0	22.0	41.0
20	35.11710861393674	40.0	35.0	41.0	25.0	41.0
21	35.148058138126146	40.0	36.0	41.0	24.0	41.0
22	34.744810317742385	40.0	35.0	41.0	18.0	41.0
23	33.05825847243929	39.0	32.0	41.0	10.0	41.0
24	34.1857088175192	39.0	34.0	41.0	16.0	41.0
25	33.92493772381291	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7179293650401064
1101	2	1.1026408059054909
1101	3	0.8234539085530663
1101	4	0.7674397514981095
1101	5	1.0389833233109727
1101	6	0.7273431439241378
1101	7	0.6807507561010979
1101	8	0.739340718609995
1101	9	0.8524841212697538
1101	10	1.2387275730316958
1101	11	0.935934844016721
1101	12	0.9958180405081478
1101	13	0.9128947428691561
1101	14	0.10950274211585764
1101	15	0.6639077483696525
1101	16	1.3486555250987777
1101	17	1.906003499390998
1101	18	-4.415114756202868
1101	19	-0.6002667215438251
1101	20	0.354401591322862
1101	21	0.7082745874173284
1101	22	1.2560960400901138
1101	23	3.114006449545812
1101	24	1.965408597114326
1101	25	2.1592804084101758
1102	1	0.9652402991626339
1102	2	1.2352114925341482
1102	3	1.070241565775504
1102	4	1.631278082582817
1102	5	1.4868675843836385
1102	6	1.119438137468407
1102	7	0.9278683084360395
1102	8	0.8371340191569203
1102	9	0.893552145816038
1102	10	1.2768073919810803
1102	11	1.0162096115378674
1102	12	1.0981505179130906
1102	13	0.9206609148382228
1102	14	1.8795015815349814
1102	15	1.3715069417659436
1102	16	1.6600929045071737
1102	17	1.4140846239938654
1102	18	1.6666013076871096
1102	19	1.5567607407010229
1102	20	1.3718548416746685
1102	21	1.3867672830114728
1102	22	1.761645502596096
1102	23	3.3860476211522013
1102	24	2.2651405029331926
1102	25	2.4970097319366005
1103	1	0.9099102030633084
1103	2	1.2308951643716668
1103	3	0.9140000526109127
1103	4	1.495556756092256
1103	5	1.3279659662512486
1103	6	0.8842928254000171
1103	7	0.8402728269944078
1103	8	0.7876367596596623
1103	9	0.9135137642391982
1103	10	1.2631944352912043
1103	11	0.8938747322799117
1103	12	1.066793351171306
1103	13	0.9201347673890012
1103	14	1.7430729581832836
1103	15	1.2578471711830943
1103	16	1.5491618604992041
1103	17	1.9539531961701258
1103	18	1.9996325799491501
1103	19	1.357982263461011
1103	20	1.3609871854223954
1103	21	1.336169897029471
1103	22	1.6153696786279639
1103	23	3.1621549441826033
1103	24	2.063001020793706
1103	25	2.148364572522212
1104	1	0.6120577068388577
1104	2	1.0835388494044444
1104	3	0.8148534789383355
1104	4	1.3418260248350933
1104	5	1.0999939811872466
1104	6	0.7227279468204344
1104	7	0.6287829587949858
1104	8	0.6134512004701165
1104	9	0.7829618054091654
1104	10	1.0716552257228287
1104	11	0.7798022443195478
1104	12	0.8817520614933656
1104	13	0.6655820470845342
1104	14	1.577014965698197
1104	15	0.949060065216436
1104	16	1.1837429544507572
1104	17	1.7268724823121673
1104	18	2.2573104347881525
1104	19	1.4286836766654432
1104	20	1.0402237343642184
1104	21	1.0725720934054834
1104	22	1.4546044626196704
1104	23	2.8952294086296533
1104	24	1.8927981845446098
1104	25	1.9217929623885368
1105	1	0.5751450935469258
1105	2	0.9371480031772812
1105	3	0.7168968646074596
1105	4	0.9013499841373687
1105	5	0.9126648958907282
1105	6	0.6470975003012711
1105	7	0.4741248804654816
1105	8	0.514181508286427
1105	9	0.6727114944391133
1105	10	1.1454873021751837
1105	11	0.7774043709660674
1105	12	0.7834388346209664
1105	13	0.670990297848995
1105	14	1.5330077059559315
1105	15	0.8546671998157933
1105	16	1.137310911157357
1105	17	1.642675296685482
1105	18	1.5333478333926251
1105	19	1.162613186206471
1105	20	0.992747785091268
1105	21	0.7534194556888778
1105	22	1.252258228887058
1105	23	2.7153058699372608
1105	24	1.7046241190414122
1105	25	1.649870447541801
1106	1	-0.48541431090873033
1106	2	-0.05990845243733389
1106	3	-0.29047523798477926
1106	4	-2.7421173489038146
1106	5	-0.46201493546388406
1106	6	-0.3842949169009131
1106	7	-0.3697775028046806
1106	8	-0.26098985232856364
1106	9	-0.24884732682733102
1106	10	-0.09261251573150986
1106	11	-0.19067382545161848
1106	12	-0.08168611617593058
1106	13	-0.15405405463814503
1106	14	0.612013117461153
1106	15	0.0190816745050455
1106	16	-1.01508378862286
1106	17	-5.58487470649251
1106	18	-6.864458851093094
1106	19	-2.8539586425210075
1106	20	-1.3745989716296236
1106	21	-1.015535029905898
1106	22	-1.3145512243535435
1106	23	-5.142778437143637
1106	24	-2.0326374340706224
1106	25	-0.5690164639305237
1107	1	0.5394544872955294
1107	2	0.8400311480416605
1107	3	0.6027379391681791
1107	4	1.0290483176433085
1107	5	0.9498419657478081
1107	6	0.47967643725855424
1107	7	0.4828761250071736
1107	8	0.43973010664618073
1107	9	0.42368918227928987
1107	10	0.8910076370411488
1107	11	0.5535254995989334
1107	12	0.6437057761416156
1107	13	0.4996957443524721
1107	14	1.4240944860601417
1107	15	0.7971190989987349
1107	16	-5.665350668299613
1107	17	-0.9248661245660585
1107	18	0.8195313247286435
1107	19	0.7413757569679476
1107	20	0.660604471971979
1107	21	0.7394870272476268
1107	22	0.8629455885079622
1107	23	2.718689763033531
1107	24	1.6337564769061217
1107	25	1.3070633916411225
1108	1	0.4927515604238941
1108	2	0.7612714587503646
1108	3	0.40619595766739636
1108	4	1.0234993663666003
1108	5	0.7991437668160728
1108	6	0.3365411780089502
1108	7	0.3199516417693715
1108	8	0.4114639741118751
1108	9	0.4334888012214435
1108	10	0.9341595879270272
1108	11	0.6331113570333642
1108	12	0.6235840764716514
1108	13	0.4801245972706596
1108	14	1.2348281581115614
1108	15	0.7467068291533323
1108	16	1.0760965644170781
1108	17	1.0588819212911602
1108	18	0.8628316004799004
1108	19	0.8798195807911142
1108	20	0.6434863168998888
1108	21	0.7671065285069645
1108	22	1.0628391962897936
1108	23	2.682566483764816
1108	24	1.455423442122381
1108	25	1.6394463873420122
1109	1	0.2658245444687424
1109	2	0.6516993775731308
1109	3	0.46697175003479785
1109	4	0.5150359931453821
1109	5	0.3041253725728623
1109	6	0.19969407257556782
1109	7	0.22491876121740972
1109	8	0.049091992654354044
1109	9	0.18255392897047784
1109	10	0.8193380727265094
1109	11	0.3852799237647133
1109	12	0.43009415624741365
1109	13	0.2818255594310699
1109	14	-0.7023133899695111
1109	15	-0.03544805980742893
1109	16	-0.20723306311634104
1109	17	0.7964789458707173
1109	18	1.6199676235501883
1109	19	0.6858590239778337
1109	20	0.4377312096338244
1109	21	0.4486189665190423
1109	22	0.3873918051729319
1109	23	-4.262715359163174
1109	24	-1.425049350568436
1109	25	-0.09908550948851769
1110	1	0.7232393653019429
1110	2	1.0152285217584947
1110	3	0.8003143519763398
1110	4	1.3664236549844873
1110	5	1.0727146509624106
1110	6	0.7255274584967495
1110	7	0.6114658694116528
1110	8	0.6352190554126906
1110	9	0.7028803154490859
1110	10	1.0913305736993877
1110	11	0.6327620176024666
1110	12	0.701353385414734
1110	13	0.5978900952381352
1110	14	1.3856411662416406
1110	15	0.8132337600476376
1110	16	1.2847792371813114
1110	17	1.6312904578765242
1110	18	2.2942213362522565
1110	19	1.508293920222009
1110	20	1.0594987660868824
1110	21	0.993268876067944
1110	22	1.3012030174280014
1110	23	2.793650071163185
1110	24	1.7295024563500192
1110	25	1.9882586332769705
1111	1	0.34179512827163094
1111	2	0.7356260263644359
1111	3	0.5617867842032105
1111	4	0.9822502426092683
1111	5	0.7121549747564231
1111	6	0.31814844604361525
1111	7	0.24140896609599594
1111	8	0.1166271676712256
1111	9	0.23003580765784903
1111	10	0.5102781913555248
1111	11	0.23576152644027815
1111	12	0.27909670498723926
1111	13	0.14779383159388004
1111	14	-0.4072245863866968
1111	15	-0.001958759469324889
1111	16	0.44864076156637367
1111	17	0.9948843429072696
1111	18	1.5966419550129984
1111	19	0.7264718691121317
1111	20	0.5189570318077017
1111	21	0.39753000596894594
1111	22	0.6241326366840454
1111	23	0.667244650452723
1111	24	0.49879566204335646
1111	25	0.010199995868646283
1112	1	0.32519428846088516
1112	2	0.5745172421677651
1112	3	0.38362147784394196
1112	4	0.7586684237436501
1112	5	0.6253151492246474
1112	6	0.07687653425106333
1112	7	0.01238281032759403
1112	8	-0.05079038696420923
1112	9	0.07931064075486205
1112	10	0.41917200123093323
1112	11	0.11070468668867761
1112	12	0.06615842493739166
1112	13	-0.10099240304460011
1112	14	0.34497685504304343
1112	15	0.18904144979225634
1112	16	0.5693260594780298
1112	17	0.7098434597526975
1112	18	0.8733369336030634
1112	19	0.3246398177604277
1112	20	0.27848822158673414
1112	21	0.27964823458094656
1112	22	0.12412019671013752
1112	23	1.8054992417513631
1112	24	0.7575327186040965
1112	25	0.7942688573924528
1113	1	0.41750098414991754
1113	2	0.8182664343616253
1113	3	0.5417136009126438
1113	4	0.6615523578459772
1113	5	0.8625724297575417
1113	6	0.38830666022678173
1113	7	0.25813751070820956
1113	8	0.240501750929198
1113	9	0.3466384600878314
1113	10	0.5644377044372746
1113	11	0.2921825911858278
1113	12	0.41248745796502106
1113	13	0.29343170242007943
1113	14	1.0655924414737044
1113	15	0.5058079703816176
1113	16	0.870856523151744
1113	17	1.3197642379125512
1113	18	1.8352902065143155
1113	19	0.9458365885913977
1113	20	0.6512424115117312
1113	21	0.5881440660676844
1113	22	0.8620560811173235
1113	23	0.506597172926746
1113	24	0.4905657998032851
1113	25	0.9703133838226208
1114	1	-0.09658846390700404
1114	2	-2.8072827535367395
1114	3	-0.8206402649603426
1114	4	0.3976896035928803
1114	5	0.6528523282515977
1114	6	0.40693731547853673
1114	7	0.4218829069761867
1114	8	0.4174174412651581
1114	9	0.534091371172785
1114	10	0.622801999727514
1114	11	0.35131581262946554
1114	12	0.4402127261066866
1114	13	0.32844614532418603
1114	14	1.1736207371869867
1114	15	0.6247824070122121
1114	16	0.8870587754876382
1114	17	1.2964427485417716
1114	18	1.4937736625236937
1114	19	0.8941501008641097
1114	20	0.7178343248074341
1114	21	0.6577372310617093
1114	22	0.7876263009126632
1114	23	2.3853512313317253
1114	24	1.206105847840142
1114	25	1.035943117877288
1115	1	0.11284230470630874
1115	2	0.36945544004369424
1115	3	0.16993646985960353
1115	4	0.3731493633783174
1115	5	0.33664880954855647
1115	6	-0.2298133372910769
1115	7	-0.299380077889559
1115	8	-0.27697708692884504
1115	9	-0.24849164791793044
1115	10	-0.03635256692594879
1115	11	-0.08317735372047963
1115	12	-0.2291399951675288
1115	13	-0.28501088414220277
1115	14	0.5266487215030651
1115	15	-0.18150570272171507
1115	16	0.3441274458618153
1115	17	0.8103013571027802
1115	18	1.2207033681506303
1115	19	0.4286071460353611
1115	20	-0.10536873380136313
1115	21	-0.034560872779991314
1115	22	0.35685565178425094
1115	23	2.056590746156239
1115	24	0.7022952670685925
1115	25	0.42181269890070183
1116	1	0.22088351148840246
1116	2	0.10164696518506489
1116	3	0.17993136043193303
1116	4	0.7427930389118842
1116	5	0.22011984522407602
1116	6	0.026279917284448118
1116	7	-0.04781329308922011
1116	8	-0.12104416856368516
1116	9	-0.05823024668131893
1116	10	0.31830147901377615
1116	11	-0.08609915099015097
1116	12	0.03578120866103518
1116	13	0.014847398748258911
1116	14	0.49961803736164256
1116	15	-0.13910058663948632
1116	16	0.33921793993344806
1116	17	0.7183790614246206
1116	18	1.3118758291103418
1116	19	0.3731075832537769
1116	20	0.15432823976293264
1116	21	0.07565013154160027
1116	22	0.3714942646088275
1116	23	1.908228076154387
1116	24	0.7669798351157056
1116	25	0.9707373489721363
1201	1	0.9492152063429167
1201	2	1.3099388137681878
1201	3	1.0485496901872757
1201	4	1.6528244109237704
1201	5	1.4440957393277216
1201	6	1.066065872963705
1201	7	0.9280821828533732
1201	8	1.0202774395172298
1201	9	1.2022150231161532
1201	10	1.4592326359802215
1201	11	1.0603185792936642
1201	12	1.2235055301778885
1201	13	1.1036527111313674
1201	14	1.9290979917647917
1201	15	1.3895752541144901
1201	16	1.7685596190967274
1201	17	2.247718636399469
1201	18	2.7811224616900247
1201	19	1.8599337299776622
1201	20	1.5063872600266635
1201	21	1.4700536568544678
1201	22	1.7322946848191734
1201	23	3.346227183769386
1201	24	2.185111156339545
1201	25	2.430454621849826
1202	1	1.0216724179351786
1202	2	1.4105959872803453
1202	3	1.1183423551528904
1202	4	1.6806711506992897
1202	5	1.4392254105712539
1202	6	1.0838526763510288
1202	7	0.9541352330008408
1202	8	0.9307064559208484
1202	9	1.1104690184775947
1202	10	1.6253175821933965
1202	11	1.1920274796961579
1202	12	1.1828802103023577
1202	13	1.0970364254690566
1202	14	2.082758640749489
1202	15	1.4707447482377916
1202	16	1.6674118592729315
1202	17	2.1818267955658186
1202	18	2.798006055900366
1202	19	2.1198341595191224
1202	20	1.7304695851830232
1202	21	1.6785729931150541
1202	22	2.078263576873745
1202	23	3.6801753852799663
1202	24	2.4331415185937786
1202	25	2.5930810671568736
1203	1	0.9493333781882001
1203	2	1.328509549477836
1203	3	0.9941312532033564
1203	4	1.6594377274853684
1203	5	1.460360915135169
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2211	16	-0.6333406660496905
2211	17	-0.151449128937152
2211	18	-5.862051866034559
2211	19	-2.0624135020348575
2211	20	-1.3640411913446542
2211	21	-1.0669406715184024
2211	22	-0.5630423359273564
2211	23	-5.535013028538586
2211	24	-2.537359805594516
2211	25	-1.7956163187716356
2212	1	0.3177780120914093
2212	2	0.479477480207958
2212	3	0.2345153774236195
2212	4	0.8631268739846831
2212	5	0.6903056034082482
2212	6	0.21635247181291106
2212	7	0.10641579446956229
2212	8	0.03061445235802296
2212	9	-0.2828809454773875
2212	10	-3.858313193307101
2212	11	-1.1954626600226703
2212	12	-0.3789875546439774
2212	13	-0.27294525100257516
2212	14	0.6015614829803582
2212	15	0.16604855295560128
2212	16	0.4443163407417785
2212	17	0.9335962432820182
2212	18	1.5230998732918195
2212	19	0.7221310068534166
2212	20	0.22950092351069173
2212	21	0.18563843450831996
2212	22	0.37324845648955574
2212	23	1.794446588936644
2212	24	0.8336186712996252
2212	25	0.8922678544572946
2213	1	-1.5114151062427723
2213	2	-4.014322291249638
2213	3	-1.7856165423326047
2213	4	-3.5149381336334002
2213	5	-1.3339499831839277
2213	6	-0.9402713219910552
2213	7	-0.9213816915639583
2213	8	-0.8599943592214601
2213	9	-0.8322924487785528
2213	10	-0.8454966620729749
2213	11	-0.9143782262999736
2213	12	-0.7449575901950212
2213	13	-1.1334066527293416
2213	14	-6.510255175221772
2213	15	-2.940689004180001
2213	16	-1.9974138522495863
2213	17	-0.8626180787088416
2213	18	-0.04818923285343146
2213	19	-0.7434825025422143
2213	20	-0.8270032664334437
2213	21	-1.0983205548263655
2213	22	-1.0393274703768753
2213	23	-5.501006432901853
2213	24	-2.8864203078776214
2213	25	-1.962584862657991
2214	1	-0.14648269026615068
2214	2	-0.011061331678277497
2214	3	-0.17433025397410518
2214	4	0.15075597255266615
2214	5	-0.10404630563887451
2214	6	-0.29058147786051336
2214	7	-0.3742651547885103
2214	8	-0.4888186407606696
2214	9	-0.3962104955393926
2214	10	-0.03683750474223757
2214	11	-0.5605273982434653
2214	12	-0.44051774606447935
2214	13	-0.5834823955447348
2214	14	-0.05688011433526441
2214	15	-0.3078827450470243
2214	16	0.008319099528300455
2214	17	0.31634051846602773
2214	18	0.7819253555537742
2214	19	-0.27526964331182313
2214	20	-0.42593178567357626
2214	21	-0.47071984854287763
2214	22	0.03202859391927859
2214	23	1.4752424711410086
2214	24	0.4286794242217482
2214	25	0.21670641839412497
2215	1	-2.273701227915776
2215	2	-4.276623622048117
2215	3	-2.229349432066442
2215	4	-3.9756985899071644
2215	5	-4.862182872917767
2215	6	-2.192445276363678
2215	7	-1.611361729586772
2215	8	-1.9011112793735485
2215	9	-1.4447401674097335
2215	10	-2.971837916825823
2215	11	-1.9172936255395143
2215	12	-1.5149100616779094
2215	13	-1.5982944854289585
2215	14	-0.8565137303548553
2215	15	-1.373003642963269
2215	16	-2.2209319130860905
2215	17	-6.737220740305798
2215	18	-2.8668955066176913
2215	19	-2.0132600690821434
2215	20	-1.843610680882115
2215	21	-1.6509850034955704
2215	22	-1.5221734779814398
2215	23	-6.229023543168015
2215	24	-3.3186646044555665
2215	25	-2.8916342923157856
2216	1	-0.934893238630476
2216	2	-0.4438205181999919
2216	3	-0.7358807267297678
2216	4	-2.8679641383615326
2216	5	-1.2304162952406656
2216	6	-0.8969953172629204
2216	7	-1.0508078144953998
2216	8	-0.8711970778975839
2216	9	-0.9087965858943932
2216	10	-0.482778576966588
2216	11	-1.094346902422906
2216	12	-1.7644256753014034
2216	13	-1.223478756748257
2216	14	-7.164418295789147
2216	15	-3.3027132358584907
2216	16	-1.719997185582919
2216	17	-0.9380743434842529
2216	18	-0.1406295401820401
2216	19	-0.5800522424949364
2216	20	-1.0190192238802496
2216	21	-1.1939547155828691
2216	22	-0.738254184324866
2216	23	0.5118753637884552
2216	24	-0.2581052960572876
2216	25	-0.13980708488021065
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	100920.0
3	4.0
4	850.0
5	5362.0
6	6345.0
7	5818.0
8	5349.0
9	5036.0
10	4849.0
11	4745.0
12	4835.0
13	4655.0
14	4658.0
15	4933.0
16	4994.0
17	5441.0
18	6059.0
19	7088.0
20	8832.0
21	11075.0
22	13684.0
23	16835.0
24	19786.0
25	23370.0
26	26886.0
27	31182.0
28	34384.0
29	41866.0
30	49970.0
31	56255.0
32	73591.0
33	97319.0
34	128871.0
35	172158.0
36	245555.0
37	347501.0
38	903436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67211678055656	17.810944792514093	12.057339420892157	25.45959900603719
2	14.255812052440856	19.71106550033	41.41352884492643	24.619593602302714
3	19.097728315925945	24.40429695364025	28.881109026340585	27.616865704093218
4	10.77404611132942	16.245337653720537	39.12980809492827	33.850808140021776
5	12.500956260060864	37.79433684673346	36.33800310351686	13.366703789688819
6	32.70705643416283	36.20920716009573	18.014166410028803	13.069569995712637
7	28.78121735349709	31.12494187790233	21.76312754252726	18.33071322607332
8	25.95915892655201	35.920788141007705	20.19202704111765	17.92802589132263
9	26.957459640567745	14.958092540238649	19.413392189245055	38.671055629948555
10	14.145556024852423	28.907953347149185	34.953682546572125	21.992808081426272
11	34.90700028099997	22.516149446897042	23.558184305386582	19.0186659667164
12	23.358927794312482	24.704941745192535	31.320599307327168	20.61553115316781
13	29.06093970841078	21.361726589512305	25.386184433787378	24.191149268289543
14	20.99851809267537	21.299891584449192	28.20016095260755	29.50142937026789
15	24.193710718156243	29.206931479098795	23.059841254249243	23.539516548495715
16	22.628748380115244	27.68850547087267	27.21205101665759	22.4706951323545
17	22.20185532461739	27.756194144800993	27.817106959359517	22.224843571222095
18	21.83189426121011	26.95281174617795	29.431749012080477	21.783544980531463
19	23.591811426880408	27.00224060425856	27.455394259785283	21.950553709075756
20	23.84657160996666	26.738128950155822	27.502847312776858	21.91245212710066
21	23.455114082892077	26.382393486130972	27.23652552854327	22.925966902433682
22	23.214469776520836	26.973963936686275	26.89689937961824	22.914666907174656
23	21.459785071607904	26.611882419063658	29.019721362877267	22.908611146451168
24	22.97672849209519	27.122001069436035	27.25455315612478	22.646717282344
25	22.9088248900155	26.487191390146954	27.330026786012652	23.2739569338249
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	244.0
1	244.0
2	436.5
3	629.0
4	629.0
5	629.0
6	1220.0
7	1811.0
8	1811.0
9	1811.0
10	2755.0
11	3699.0
12	3699.0
13	3699.0
14	6712.0
15	9725.0
16	9725.0
17	9725.0
18	17779.0
19	25833.0
20	25833.0
21	25833.0
22	40709.0
23	55585.0
24	55585.0
25	55585.0
26	78398.5
27	101212.0
28	101212.0
29	101212.0
30	131288.0
31	161364.0
32	161364.0
33	161364.0
34	188693.5
35	216023.0
36	216023.0
37	216023.0
38	241120.0
39	266217.0
40	266217.0
41	266217.0
42	286826.5
43	307436.0
44	307436.0
45	307436.0
46	328923.5
47	350411.0
48	350411.0
49	350411.0
50	336476.5
51	322542.0
52	322542.0
53	322542.0
54	288017.0
55	253492.0
56	253492.0
57	253492.0
58	217312.5
59	181133.0
60	181133.0
61	181133.0
62	148666.0
63	116199.0
64	116199.0
65	116199.0
66	89465.0
67	62731.0
68	62731.0
69	62731.0
70	45961.5
71	29192.0
72	29192.0
73	29192.0
74	20504.0
75	11816.0
76	11816.0
77	11816.0
78	8239.5
79	4663.0
80	4663.0
81	4663.0
82	3196.5
83	1730.0
84	1730.0
85	1730.0
86	1152.0
87	574.0
88	574.0
89	574.0
90	383.5
91	193.0
92	193.0
93	193.0
94	113.0
95	33.0
96	33.0
97	33.0
98	21.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10923740298338054
2	0.04693102869514433
3	0.03529889551084183
4	0.030951939165150936
5	0.034735401169733754
6	0.01819281729863228
7	0.02020529708830399
8	0.023183767177018123
9	0.027812470693263064
10	0.035258645915048396
11	0.0205675434504449
12	0.019963799513543386
13	0.01553634397626562
14	0.021573783345280755
15	0.021131037791552978
16	0.021090788195759543
17	0.0249144997961358
18	0.019682052342989344
19	0.03187767986839992
20	0.023183767177018123
21	0.027731971501676195
22	0.03481590036132062
23	0.027892969884849933
24	0.03803586802479536
25	0.029704201695554475
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2484497.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.43012429054168
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.31598569782653	60.34587199482569
2	11.161351219438863	16.168361121486917
3	2.929422251377988	6.365352533003583
4	1.0855238653098886	3.144985139389779
5	0.5000359386238127	1.810883259213021
6	0.27061624249540234	1.1760460847388818
7	0.17279972677158556	0.8761133981206309
8	0.11670704601893965	0.6762484678987014
9	0.07678250010818391	0.5005229423556862
>10	0.3400209674717252	4.152379986454968
>50	0.018650610890072257	0.9359467927716938
>100	0.009744590446044019	1.3767429512300224
>500	0.0012358464489737062	0.6133234565573376
>1k	0.001011147094605868	1.2811274828503225
>5k	1.1234967717842978E-4	0.5760943891027318
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7791	0.3135846008266462	No Hit
CCCATGTACTCTGCGTTGATACCAC	6368	0.25630942601258927	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3242	0.1304891895623138	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3080	0.12396875504377747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.024959579343424E-5	0.0
5	0.0	0.0	0.0	4.024959579343424E-5	4.024959579343424E-5
6	0.0	0.0	0.0	4.024959579343424E-5	4.024959579343424E-5
7	0.0	0.0	0.0	8.049919158686848E-5	4.024959579343424E-5
8	0.0	0.0	0.0	8.049919158686848E-5	4.024959579343424E-5
9	0.0	0.0	0.0	8.049919158686848E-5	4.024959579343424E-5
10	0.0	0.0	0.0	8.049919158686848E-5	4.024959579343424E-5
11	0.0	0.0	0.0	8.049919158686848E-5	4.024959579343424E-5
12	0.0	0.0	0.0	8.049919158686848E-5	2.4149757476060548E-4
13	0.0	0.0	0.0	1.2074878738030274E-4	3.2199676634747394E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGGCGT	45	6.770323E-4	14.776055	6
GTAGGAC	1185	0.0	12.347784	3
TAGGACC	670	0.0	11.912177	4
TGTAGGA	1295	0.0	11.665786	2
GTCCTAC	1130	0.0	11.53261	1
CTGTAGG	1305	0.0	11.152349	1
TGGCGAG	1075	0.0	11.1347065	18
TATGGCG	1110	0.0	10.782961	16
GGCGAGA	690	0.0	10.738744	19
GTATTAG	235	0.0	10.524244	1
GCGTTTA	155	3.6725396E-9	10.418141	13
TTAGGAC	640	0.0	10.392134	3
GTCCTAA	605	0.0	10.377053	1
TCCTACA	1355	0.0	10.30777	2
TAGGACG	750	0.0	10.26149	4
ATGGCGA	1180	0.0	10.223796	17
CGTTTAT	160	6.188202E-9	10.092573	14
TAGGACT	445	0.0	10.035165	4
AGGACGT	805	0.0	10.031502	5
AGGACCT	1055	0.0	9.995706	5
>>END_MODULE