FastQCFastQC Report
Thu 26 May 2016
SRR1513072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2484497
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC92300.3715037691733981No Hit
CCCATGTACTCTGCGTTGATACCAC84260.33914309415547694No Hit
GTATCAACGCAGAGTACTTTTTTTT46990.18913285063334753No Hit
GAGTACATGGGAAGCAGTGGTATCA34360.13829761114624006No Hit
TATCAACGCAGAGTACTTTTTTTTT31200.12557873887551485No Hit
GGTATCAACGCAGAGTACTTTTTTT27980.11261836903002903No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCGAA456.796674E-414.76848616
TAGGACC5400.011.6340474
TCGAACT2050.011.10667819
GTCCTAC12200.010.9328911
TCCAACG1408.481948E-910.84703818
ATGGCGA9600.010.68034917
GTAGGAC11450.010.5579323
AGGACGT8500.010.5266355
TATGGCG9400.010.50402916
TGGCGAG9450.010.44529518
GGACGTG8500.010.3026646
CGAGGTT750.002605514810.1533514
GATCGCA750.002679332210.11921411
CTAGGAC3200.010.1136883
TAGGACG9250.010.0847464
GGCGAGG4250.010.04501119
CCCGTTT951.655192E-49.99371212
CCAACGA1054.169366E-59.93871419
GCATTTC9800.09.88153713
TCCTACA14550.09.8193092
Produced by FastQC (version 0.11.5)