FastQCFastQC Report
Thu 26 May 2016
SRR1513071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038995
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC38250.18759241685241992No Hit
CCCATGTACTCTGCGTTGATACCAC28850.1414912738873808No Hit
GTCCTACAGTGGACATTTCTAAATT25350.12432595469827049No Hit
GTATCAACGCAGAGTACTTTTTTTT24030.1178521771755203No Hit
GTCCTACAGTGTGCATTTCTCATTT20520.10063781421729823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGC400.005273882314.2524294
GTCCTAC13650.013.1696621
TAGGACC7300.012.8857584
TGTAGGA18150.012.5108542
GTAGGAC16550.012.4567883
GGCGTGT701.0927356E-412.2124728
TCCTACA16300.011.7158582
CCAACGA1406.8030204E-1011.535719
GTCTTAG3150.011.4740981
TCCAACG2000.011.40026418
GGCGAGG4850.011.36080919
TGGCGAG13500.011.32989218
GTCTTAT2450.011.2584191
CTGTAGG17600.011.2407731
GACGTGG5950.011.0151717
AAACCGT700.001492679710.856336
AGGCGTG1502.1391315E-910.7650687
AGGACGT12150.010.7127095
AGACCCG803.7692412E-410.6882725
TAGGACA5800.010.6483664
Produced by FastQC (version 0.11.5)