##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513071_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2038995
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46546018994652	31.0	30.0	34.0	25.0	34.0
2	29.18536730104782	31.0	30.0	34.0	20.0	34.0
3	29.573160797353598	31.0	30.0	34.0	25.0	34.0
4	32.45516443149689	35.0	33.0	37.0	19.0	37.0
5	32.70816161883673	35.0	33.0	37.0	25.0	37.0
6	33.16421815649376	37.0	35.0	37.0	27.0	37.0
7	33.147211248678886	37.0	35.0	37.0	27.0	37.0
8	33.14712247945679	37.0	35.0	37.0	27.0	37.0
9	34.555332406406094	39.0	35.0	39.0	27.0	39.0
10	34.051979038693084	39.0	34.0	39.0	23.0	39.0
11	34.43712809496836	39.0	35.0	39.0	27.0	39.0
12	34.26415415437507	39.0	35.0	39.0	26.0	39.0
13	34.36927702127764	39.0	35.0	39.0	27.0	39.0
14	34.67642490540683	40.0	34.0	41.0	17.0	41.0
15	35.2634018229569	40.0	35.0	41.0	25.0	41.0
16	34.86976574243684	40.0	34.0	41.0	19.0	41.0
17	34.416856343443705	39.0	34.0	41.0	17.0	41.0
18	33.844572448681824	39.0	33.0	41.0	11.0	41.0
19	34.72676735352465	39.0	34.0	41.0	19.0	41.0
20	34.97258845656806	40.0	35.0	41.0	23.0	41.0
21	34.999102499025255	40.0	35.0	41.0	22.0	41.0
22	34.59284010014738	40.0	34.0	41.0	18.0	41.0
23	32.938807598841585	39.0	32.0	41.0	9.0	41.0
24	34.05390989188301	39.0	34.0	41.0	10.0	41.0
25	33.77875522009617	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8221481798018004
1101	2	1.156180486498645
1101	3	0.982005219350512
1101	4	0.9636229395130584
1101	5	1.116378250641226
1101	6	0.8722109293033427
1101	7	0.8694674731756962
1101	8	0.8527223439143086
1101	9	0.9865316104565807
1101	10	1.2757410838297574
1101	11	0.959086054256332
1101	12	0.9254765315304851
1101	13	0.9292906959270439
1101	14	0.11551549562471308
1101	15	0.567097025010483
1101	16	1.1784195982002572
1101	17	1.840976507508266
1101	18	-4.295654053364984
1101	19	-0.6530524509105362
1101	20	0.30960165912780724
1101	21	0.7466060065534634
1101	22	1.2404003936948556
1101	23	3.0181156775717355
1101	24	1.9364983736526824
1101	25	2.1623155032958294
1102	1	1.006554306902558
1102	2	1.1983726484115422
1102	3	1.0699430965047974
1102	4	1.5448599703981856
1102	5	1.3304699228624841
1102	6	0.903582279200478
1102	7	0.8375387619291175
1102	8	0.8030710637184768
1102	9	0.965917196243474
1102	10	1.3136860461836548
1102	11	0.8996674603173673
1102	12	1.0477821811614234
1102	13	0.9321849624958887
1102	14	1.8176572782517084
1102	15	1.2103894509724498
1102	16	1.4952285626553845
1102	17	1.1981249896555823
1102	18	1.6120370374663509
1102	19	1.3060476722416468
1102	20	1.315848164809978
1102	21	1.215174642882559
1102	22	1.6178245379566647
1102	23	3.1966171641953522
1102	24	2.0728963642462546
1102	25	2.232348637125156
1103	1	0.7332127077534096
1103	2	1.07735767034114
1103	3	0.828422255085961
1103	4	1.239389763397888
1103	5	1.1630316274065038
1103	6	0.7948739789246844
1103	7	0.7569232448634153
1103	8	0.7375924077263889
1103	9	0.7988281944723923
1103	10	1.142806790061286
1103	11	0.877762475984639
1103	12	0.9680809411745699
1103	13	0.7863297195988252
1103	14	1.5918211085529066
1103	15	1.114850243872553
1103	16	1.2754129292729388
1103	17	1.7701916840276155
1103	18	1.6616309019871167
1103	19	1.2419398594429154
1103	20	1.1819168764074206
1103	21	1.2284550154624938
1103	22	1.5496978442944425
1103	23	2.9406985767568727
1103	24	1.7798032433054658
1103	25	1.9650378779408868
1104	1	0.7564680005594511
1104	2	1.1639641679293362
1104	3	0.9159895531357876
1104	4	1.5044127049599325
1104	5	1.2486560356598702
1104	6	0.8928957944173845
1104	7	0.7547641049338907
1104	8	0.84545808614115
1104	9	0.9730171164150576
1104	10	1.3239364449014204
1104	11	0.9748100454115729
1104	12	1.0922298064216065
1104	13	0.8069664810009769
1104	14	1.7371484810017463
1104	15	1.209872008944231
1104	16	1.491848499618655
1104	17	1.9469279877189933
1104	18	2.4475617579678826
1104	19	1.6720742594608424
1104	20	0.9393531358403493
1104	21	1.155783205852245
1104	22	1.5252486938885497
1104	23	2.8917229637213637
1104	24	2.031664619126907
1104	25	2.108813963813482
1105	1	0.6178116952300954
1105	2	0.9471999783129448
1105	3	0.6612008806703429
1105	4	1.0281841824081326
1105	5	0.9000541306160628
1105	6	0.5211366051873085
1105	7	0.4769460145264688
1105	8	0.4983510735210217
1105	9	0.7777081208343333
1105	10	1.1971366594990656
1105	11	0.6823430539465107
1105	12	0.6974925633919469
1105	13	0.6983467726230757
1105	14	1.3913947829467261
1105	15	0.7906659549400601
1105	16	1.156177408598566
1105	17	1.5759858365843584
1105	18	1.5409783331331042
1105	19	1.1063586274588033
1105	20	0.919704827864436
1105	21	0.7706176489920296
1105	22	1.2163863117023297
1105	23	2.716732295236639
1105	24	1.680122987510103
1105	25	1.5676273215375218
1106	1	-0.5559727184101462
1106	2	-0.10347049199795677
1106	3	-0.3602390431180815
1106	4	-2.7588691678630326
1106	5	-0.6193080048337194
1106	6	-0.5616093433638056
1106	7	-0.5479753099357367
1106	8	-0.45900377276641535
1106	9	-0.44677428492730087
1106	10	-0.34963719920179415
1106	11	-0.3775708439610099
1106	12	-0.2887946403531032
1106	13	-0.40558880293600197
1106	14	0.2224186679775073
1106	15	-0.2916541640235053
1106	16	-1.3846489218425901
1106	17	-6.3371126381872465
1106	18	-6.944753016722849
1106	19	-3.0550251518362117
1106	20	-1.6975055787605626
1106	21	-1.2511642387094497
1106	22	-1.583129434457291
1106	23	-5.299235555821394
1106	24	-2.3369487698680196
1106	25	-0.9214218150794409
1107	1	0.35781639568936896
1107	2	0.7084838412570527
1107	3	0.46023051502419676
1107	4	0.9215139681726185
1107	5	0.7669721690660651
1107	6	0.31717761419422885
1107	7	0.2889260809121197
1107	8	0.3744107343503842
1107	9	0.28635354889803466
1107	10	0.6903131802882854
1107	11	0.36743728502867157
1107	12	0.37740493399648756
1107	13	0.2750502046875809
1107	14	1.0972849001269296
1107	15	0.45737359783266385
1107	16	-5.968589052957778
1107	17	-1.171885882548203
1107	18	0.4726358221669287
1107	19	0.3679909619514845
1107	20	0.3630229354045298
1107	21	0.2948909581966177
1107	22	0.3473278557823676
1107	23	2.2766834654655597
1107	24	1.271165737312117
1107	25	1.0131511654297682
1108	1	0.34727043212503617
1108	2	0.7212799165055372
1108	3	0.424022502704144
1108	4	1.0177816256462506
1108	5	0.6344218050747088
1108	6	0.3530916456625306
1108	7	0.16539575557807495
1108	8	0.34200100361480423
1108	9	0.44310871050178946
1108	10	0.8394369826555987
1108	11	0.36450584389392304
1108	12	0.5123495939633997
1108	13	0.2642289338209096
1108	14	1.0676766099689488
1108	15	0.5383621081915919
1108	16	0.8014503696601523
1108	17	0.8495188010228887
1108	18	0.6515803645823155
1108	19	0.6488602269013981
1108	20	0.5692838193797414
1108	21	0.6296760664593251
1108	22	0.9570979180622246
1108	23	2.5874900015752047
1108	24	1.3999295544631636
1108	25	1.6093699451429089
1109	1	0.16315832091841287
1109	2	0.58974003971543
1109	3	0.4041538825267246
1109	4	0.3116857499884702
1109	5	0.09077498181238752
1109	6	0.06647814990735412
1109	7	-0.00694142210877402
1109	8	0.015500764130706557
1109	9	0.1297751197014847
1109	10	0.6442013028293232
1109	11	0.21101942919187167
1109	12	0.3178962686778988
1109	13	0.18862342524865028
1109	14	-0.8954667273365402
1109	15	-0.16279318858753555
1109	16	-0.29756545619768104
1109	17	0.6837934150314595
1109	18	1.5346852490625906
1109	19	0.7301078454977841
1109	20	0.5912287647124685
1109	21	0.5244868063358012
1109	22	0.339989197854905
1109	23	-4.197884268476063
1109	24	-1.3030683564710444
1109	25	0.06539027282404675
1110	1	0.6214275730265548
1110	2	0.8809869324347872
1110	3	0.6875310737692466
1110	4	1.1746573327080085
1110	5	0.7607961988066023
1110	6	0.54977170736543
1110	7	0.4212439106641881
1110	8	0.40896571526990755
1110	9	0.5391964156916771
1110	10	0.8013630588858192
1110	11	0.40884418421713065
1110	12	0.5630008463627405
1110	13	0.36551721590010544
1110	14	1.1552279179356688
1110	15	0.733788002588291
1110	16	1.2197340311333562
1110	17	1.4440499085796432
1110	18	1.9981814210538218
1110	19	1.195785126794192
1110	20	0.8501094403446672
1110	21	0.6834798241413225
1110	22	0.8819631429797212
1110	23	2.250307150935292
1110	24	1.3390936496819066
1110	25	1.631386221069988
1111	1	0.3895380120787131
1111	2	0.6961331171165881
1111	3	0.5499969766606831
1111	4	0.9489748449829705
1111	5	0.7056195579944387
1111	6	0.1962415002922029
1111	7	0.0855676375078076
1111	8	0.01515884129282341
1111	9	0.11990563554983424
1111	10	0.4632242984787922
1111	11	0.13623126645342865
1111	12	0.3199390090326375
1111	13	0.21097825624736544
1111	14	-0.5282804195486221
1111	15	-0.06812722310491637
1111	16	0.49206250192251133
1111	17	0.9885734504093833
1111	18	1.6274086797648906
1111	19	0.8113032368062889
1111	20	0.6098453923359344
1111	21	0.34321492222240124
1111	22	0.6183878209844167
1111	23	0.4036605037188039
1111	24	0.39634092784314845
1111	25	-0.008999936096927286
1112	1	0.50338585037181
1112	2	0.8207735812752972
1112	3	0.6143054176114582
1112	4	1.1041818447958107
1112	5	0.873214354925004
1112	6	0.20823569830830024
1112	7	0.22374413011495164
1112	8	0.282477388925237
1112	9	0.3109518642602964
1112	10	0.7504943714714898
1112	11	0.3633697654240464
1112	12	0.40325138035960606
1112	13	0.32696478067184387
1112	14	0.8419200296372296
1112	15	0.4894318261183628
1112	16	0.8167551839363583
1112	17	1.019326585352296
1112	18	1.13423836346319
1112	19	0.6242489601826691
1112	20	0.5238747765769958
1112	21	0.5462386916403261
1112	22	0.6504333010517911
1112	23	2.214024905740139
1112	24	1.2125909746333434
1112	25	1.1852411269762513
1113	1	0.49206011563421015
1113	2	0.7931340634461783
1113	3	0.48380798112194867
1113	4	0.6845726999692232
1113	5	0.7375968460842017
1113	6	0.4049306084892237
1113	7	0.31800979874434177
1113	8	0.12788904723216632
1113	9	0.23105970702025047
1113	10	0.6026595650282971
1113	11	0.37856428434356104
1113	12	0.23453753996859916
1113	13	0.17240369899442243
1113	14	0.9711640399169283
1113	15	0.5119122523581083
1113	16	0.8155808652229481
1113	17	1.3362985641942444
1113	18	1.7220097342464342
1113	19	0.7563002175642595
1113	20	0.6719880627879178
1113	21	0.6112051885465846
1113	22	0.9336835715139102
1113	23	0.8513250524097131
1113	24	0.8180113347648756
1113	25	1.2155938181277577
1114	1	-0.15615718161647862
1114	2	-2.7073465143630457
1114	3	-0.8267485000847934
1114	4	0.2506451746533003
1114	5	0.5297944941693729
1114	6	0.278568062618767
1114	7	0.27140135934152454
1114	8	0.2739560440900277
1114	9	0.319559315203513
1114	10	0.5483382955927922
1114	11	0.2792535774757354
1114	12	0.43667160976523434
1114	13	0.3076380409071504
1114	14	0.9500221667539677
1114	15	0.4347636637859722
1114	16	0.8469858008458075
1114	17	1.095492309328236
1114	18	1.178369718725932
1114	19	0.6964372139402712
1114	20	0.5280929225834683
1114	21	0.41128329829077614
1114	22	0.5186733132699075
1114	23	2.0789381149964115
1114	24	1.0290095925118123
1114	25	1.1271350400380484
1115	1	0.14996833537860255
1115	2	0.4405768183428975
1115	3	0.19843621857818405
1115	4	0.3507407312705979
1115	5	0.2970755223199646
1115	6	-0.18604389547144962
1115	7	-0.3255717158924156
1115	8	-0.2852514151174361
1115	9	-0.23763059633422046
1115	10	-0.04543121991585508
1115	11	-0.06616115272159817
1115	12	-0.19850915150247772
1115	13	-0.24870513282733242
1115	14	0.6924263808973095
1115	15	-0.04744494885742512
1115	16	0.3839041964598593
1115	17	0.8781565050359106
1115	18	1.2977141424181795
1115	19	0.5651783761596931
1115	20	0.21139054283760572
1115	21	0.13649697350444967
1115	22	0.3244368733812948
1115	23	2.012866746751129
1115	24	0.7141737037628815
1115	25	0.5281461062665045
1116	1	0.5175640449977621
1116	2	0.5571319414363707
1116	3	0.5490396370570529
1116	4	1.2443288926819989
1116	5	0.6505560712628267
1116	6	0.5033468076486045
1116	7	0.3302402945450922
1116	8	0.3080122171745785
1116	9	0.4218925351131517
1116	10	0.9367592329766055
1116	11	0.4739711052172808
1116	12	0.6012354484011269
1116	13	0.48946717126247563
1116	14	1.180053137723803
1116	15	0.5106708958067401
1116	16	0.9953668351438765
1116	17	1.251689338887715
1116	18	1.7286554046557185
1116	19	0.9381687053988372
1116	20	0.6540597020436962
1116	21	0.5690283249768484
1116	22	0.9781135515856931
1116	23	2.3683953038531627
1116	24	1.3431324965364624
1116	25	1.5916030198990399
1201	1	0.9657704470204145
1201	2	1.3198077300435962
1201	3	1.141507312260476
1201	4	1.6172966275667306
1201	5	1.4048480880728107
1201	6	0.956109335687195
1201	7	0.9779557208479659
1201	8	1.0343329763519407
1201	9	1.0902428737464973
1201	10	1.4716360840662972
1201	11	1.1392861256871996
1201	12	1.2709025545393118
1201	13	1.109073665984944
1201	14	2.0287150748398446
1201	15	1.4777217706418426
1201	16	1.8430025636357357
1201	17	2.1498745617935597
1201	18	2.6686951547382662
1201	19	1.8473991380651356
1201	20	1.6639222334926487
1201	21	1.548166507378454
1201	22	1.8838411947317226
1201	23	3.5774172006959475
1201	24	2.385705718537011
1201	25	2.410261143611436
1202	1	1.032959891237617
1202	2	1.386203918287734
1202	3	1.1640507429635818
1202	4	1.6944909482568562
1202	5	1.4720864198523032
1202	6	1.1898374545110002
1202	7	0.9223118483143011
1202	8	0.8516219767732593
1202	9	1.0468260507747473
1202	10	1.574753710243499
1202	11	1.1230888656016873
1202	12	1.1727236776512129
1202	13	1.2460817963059228
1202	14	2.0129609964748028
1202	15	1.4712691037516166
1202	16	1.8197420334636334
1202	17	2.1778297719425197
1202	18	2.742868887377348
1202	19	1.9518824223266549
1202	20	1.6768978277897588
1202	21	1.5894070968297953
1202	22	1.986302646643189
1202	23	3.576523958603019
1202	24	2.2950036906858458
1202	25	2.4030628799797285
1203	1	0.9254164028531875
1203	2	1.2404743567968382
1203	3	0.9680680027385229
1203	4	1.6872964279996125
1203	5	1.3826904530278554
1203	6	1.0164904846979184
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2211	16	-0.5989796451196341
2211	17	-0.02268755885162932
2211	18	-5.454264538908326
2211	19	-1.8911909986879465
2211	20	-1.0939567244661816
2211	21	-0.8859389239314481
2211	22	-0.5936887240921251
2211	23	-5.002141991083693
2211	24	-2.3754247119224914
2211	25	-1.5390821511791444
2212	1	0.20191667410191627
2212	2	0.542082619434396
2212	3	0.27781070468854097
2212	4	0.7660933239344274
2212	5	0.5450278927004177
2212	6	0.15531719975334113
2212	7	0.10963070078285853
2212	8	-0.0019804391269744315
2212	9	-0.28294538922838797
2212	10	-3.856056215439839
2212	11	-1.2672818285885725
2212	12	-0.31497517314492995
2212	13	-0.11093001963093485
2212	14	0.5171798247039163
2212	15	0.25571097723863545
2212	16	0.4621740862696413
2212	17	0.9156331487410299
2212	18	1.471314461349472
2212	19	0.7432343378251858
2212	20	0.27630740141893284
2212	21	0.23347832905111687
2212	22	0.4820105708255795
2212	23	1.7957495452982073
2212	24	0.9291996237510958
2212	25	0.7246593810313442
2213	1	-1.5843713079370083
2213	2	-4.097161188217395
2213	3	-1.9730730101604337
2213	4	-3.504217636115733
2213	5	-1.405987387509711
2213	6	-1.1014690728737335
2213	7	-1.111180090868416
2213	8	-0.9518602796523581
2213	9	-0.977587601600753
2213	10	-1.026533441226647
2213	11	-1.167922810908948
2213	12	-0.9583131469868746
2213	13	-1.186938277474404
2213	14	-6.291668734269667
2213	15	-2.945110044311363
2213	16	-2.0012427164539446
2213	17	-1.0331312963016757
2213	18	-0.34330848284406557
2213	19	-1.0094429193519474
2213	20	-1.164708074635925
2213	21	-1.2396735196633415
2213	22	-1.2134494082125968
2213	23	-5.582340652323833
2213	24	-3.1788973787855994
2213	25	-2.275154244667828
2214	1	-0.059994643944506265
2214	2	0.003378400135453319
2214	3	-0.15813074467458677
2214	4	0.15464683337194174
2214	5	-0.07691745216521184
2214	6	-0.32729986400387645
2214	7	-0.3148735816503958
2214	8	-0.45569166668975924
2214	9	-0.35370829768673673
2214	10	0.1465798862872063
2214	11	-0.34265407462059017
2214	12	-0.27959074423296926
2214	13	-0.42796513997905095
2214	14	0.17736254126415218
2214	15	-0.11860037259693712
2214	16	0.2859434888600987
2214	17	0.37503498887091524
2214	18	1.0061721050188837
2214	19	-0.0272860229905163
2214	20	-0.14497028255521371
2214	21	-0.2399665001288369
2214	22	0.20113382969268656
2214	23	1.57622363869703
2214	24	0.5797255432277666
2214	25	0.4029594252963946
2215	1	-2.2585999627387636
2215	2	-4.357636007843734
2215	3	-2.289416841982849
2215	4	-3.921412204006554
2215	5	-4.817511539243949
2215	6	-2.1763624569609945
2215	7	-1.5113782941396394
2215	8	-1.8215297630876677
2215	9	-1.477288312180562
2215	10	-2.861980653378154
2215	11	-1.810564628160897
2215	12	-1.5372066898352088
2215	13	-1.414975399450448
2215	14	-0.7043367098882669
2215	15	-1.3497253608681135
2215	16	-2.094768944485274
2215	17	-6.319558175873553
2215	18	-2.7458733009778875
2215	19	-1.9554401765891143
2215	20	-1.7298236373963647
2215	21	-1.6995221426384646
2215	22	-1.302550915657207
2215	23	-6.210130206258977
2215	24	-3.406984857788
2215	25	-2.9362288007673314
2216	1	-0.919236098618601
2216	2	-0.40055332610074146
2216	3	-0.7870305478459763
2216	4	-2.939433527946271
2216	5	-1.2572001843111131
2216	6	-0.9422619884283137
2216	7	-1.1694512401087351
2216	8	-0.9110593856666398
2216	9	-1.060650847477433
2216	10	-0.6920700810426297
2216	11	-1.0445378787030322
2216	12	-1.7949286764260322
2216	13	-1.2180473847782807
2216	14	-6.847838143961102
2216	15	-3.207296094300503
2216	16	-1.612140400350512
2216	17	-0.7142206080641458
2216	18	-0.06398586670144368
2216	19	-0.5858634940699545
2216	20	-1.0950059410830093
2216	21	-1.226854338511302
2216	22	-0.8146164168478691
2216	23	0.34176263975749777
2216	24	-0.48915849427062597
2216	25	-0.38787241587090904
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	88083.0
3	3.0
4	756.0
5	4592.0
6	5417.0
7	4685.0
8	4397.0
9	4266.0
10	4088.0
11	3930.0
12	3917.0
13	3879.0
14	3931.0
15	3968.0
16	4227.0
17	4558.0
18	4905.0
19	5944.0
20	7513.0
21	9218.0
22	11369.0
23	13818.0
24	16494.0
25	19629.0
26	22320.0
27	25867.0
28	28694.0
29	34807.0
30	41521.0
31	46848.0
32	61020.0
33	80615.0
34	106755.0
35	140775.0
36	201776.0
37	285330.0
38	729080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.447019474098205	18.091340011607272	12.219650465911243	25.24199004838328
2	13.832312588380876	19.48123062490003	42.856519012744506	23.829937773974585
3	19.435726526505114	23.84633612770064	28.761884483239932	27.956052862554316
4	10.490644680353165	16.134498388164204	39.63266529595621	33.74219163552642
5	12.225981394061838	37.93003295925867	36.69280970075662	13.151175945922875
6	32.65807075074869	36.719898556092154	18.003693774787656	12.6183369183715
7	28.99797846409382	30.874116835062587	21.55660717500536	18.571297525838233
8	25.158046604309774	37.48590249308186	20.02545032305918	17.330600579549184
9	26.390173281376555	15.35405161303616	19.648236990405525	38.607538115181754
10	13.65753642415298	29.51195363770528	35.37293544132885	21.457574496812885
11	35.03408551867039	22.426735879294537	23.808453954258642	18.730724647776434
12	22.992259241817752	24.778471077623422	32.05939486696492	20.16987481359391
13	29.13483196772776	21.515106030639792	25.6109907703899	23.739071231242544
14	20.16943540336565	21.768623055282347	28.692418820378364	29.36952272097364
15	23.429751559345895	30.58343941545684	23.093624525696402	22.89318449950086
16	21.97803114217065	28.31953739032495	28.11492836476624	21.58750310273816
17	21.472113342130953	28.416428329645854	28.38439387175424	21.727064456468952
18	21.03609003967491	27.57254111709788	30.32545394610334	21.065914897123864
19	23.14248385765793	27.19831936153793	27.78670963114362	21.87248714966052
20	23.173913747320945	27.35331241271151	28.361652372652625	21.111121467314923
21	22.870884848865426	26.92512983336784	27.395061377111844	22.808923940654893
22	22.461604733964705	28.04720030889895	27.713279367601036	21.77791558953531
23	21.052913019780874	27.263178705866366	29.520212835369996	22.16369543898276
24	22.440472393811977	27.35316196709729	28.299961238978867	21.906404400111867
25	22.35608564533514	27.10937917778084	28.230208020063653	22.30432715682037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	306.5
3	447.0
4	447.0
5	447.0
6	873.0
7	1299.0
8	1299.0
9	1299.0
10	2292.5
11	3286.0
12	3286.0
13	3286.0
14	5935.5
15	8585.0
16	8585.0
17	8585.0
18	15594.0
19	22603.0
20	22603.0
21	22603.0
22	36157.0
23	49711.0
24	49711.0
25	49711.0
26	70806.5
27	91902.0
28	91902.0
29	91902.0
30	118654.5
31	145407.0
32	145407.0
33	145407.0
34	169349.0
35	193291.0
36	193291.0
37	193291.0
38	212963.0
39	232635.0
40	232635.0
41	232635.0
42	247451.0
43	262267.0
44	262267.0
45	262267.0
46	272600.0
47	282933.0
48	282933.0
49	282933.0
50	270027.5
51	257122.0
52	257122.0
53	257122.0
54	225416.5
55	193711.0
56	193711.0
57	193711.0
58	164383.5
59	135056.0
60	135056.0
61	135056.0
62	109072.5
63	83089.0
64	83089.0
65	83089.0
66	63385.5
67	43682.0
68	43682.0
69	43682.0
70	31740.5
71	19799.0
72	19799.0
73	19799.0
74	13770.0
75	7741.0
76	7741.0
77	7741.0
78	5295.5
79	2850.0
80	2850.0
81	2850.0
82	1907.0
83	964.0
84	964.0
85	964.0
86	651.0
87	338.0
88	338.0
89	338.0
90	209.0
91	80.0
92	80.0
93	80.0
94	50.0
95	20.0
96	20.0
97	20.0
98	15.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1148114634905922
2	0.046150186734150896
3	0.03570386391334947
4	0.03119183715506904
5	0.035360557529567266
6	0.021088820718049822
7	0.021873521023837723
8	0.025208497323436302
9	0.031093749616845558
10	0.03859744629094235
11	0.021873521023837723
12	0.021334039563608544
13	0.01662583772888114
14	0.022560133791402134
15	0.023541009173637013
16	0.023197702789854804
17	0.027709729548135234
18	0.020941689410714594
19	0.035409601298679004
20	0.02432570947942491
21	0.03006383046549893
22	0.0386464900600541
23	0.03001478669638719
24	0.04242286028165837
25	0.033447850534209254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2038995.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.50485445494581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.87043894259462	71.54501752420728
2	8.237207230875248	13.262703384736781
3	1.6035018451619205	3.8726904798899247
4	0.5237813078207613	1.6866775180932627
5	0.22825521972109786	0.9187826621114333
6	0.13832022243014666	0.6681269624948813
7	0.08608579174756431	0.4851226894693509
8	0.058503879192017945	0.37678770235225106
9	0.04357937656853126	0.3157516231098197
>10	0.18705211588890136	2.6505390296073608
>50	0.012452762893720978	0.7093689209774205
>100	0.008793505121468478	1.4882159540233855
>500	0.0010446242342059295	0.581063025556955
>1k	9.831757498408162E-4	1.4391525233698417
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	3825	0.18759241685241992	No Hit
CCCATGTACTCTGCGTTGATACCAC	2885	0.1414912738873808	No Hit
GTCCTACAGTGGACATTTCTAAATT	2535	0.12432595469827049	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2403	0.1178521771755203	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2052	0.10063781421729823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.904376911174377E-5
2	0.0	0.0	0.0	0.0	4.904376911174377E-5
3	0.0	0.0	0.0	0.0	4.904376911174377E-5
4	0.0	0.0	0.0	0.0	4.904376911174377E-5
5	0.0	0.0	0.0	0.0	4.904376911174377E-5
6	0.0	0.0	0.0	0.0	4.904376911174377E-5
7	0.0	0.0	0.0	0.0	4.904376911174377E-5
8	0.0	0.0	0.0	0.0	4.904376911174377E-5
9	0.0	0.0	0.0	9.808753822348755E-5	4.904376911174377E-5
10	0.0	0.0	0.0	1.4713130733523132E-4	4.904376911174377E-5
11	0.0	0.0	0.0	1.4713130733523132E-4	4.904376911174377E-5
12	0.0	0.0	0.0	1.4713130733523132E-4	9.808753822348755E-5
13	0.0	0.0	0.0	1.4713130733523132E-4	1.4713130733523132E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAACGC	40	0.0052738823	14.252429	4
GTCCTAC	1365	0.0	13.169662	1
TAGGACC	730	0.0	12.885758	4
TGTAGGA	1815	0.0	12.510854	2
GTAGGAC	1655	0.0	12.456788	3
GGCGTGT	70	1.0927356E-4	12.212472	8
TCCTACA	1630	0.0	11.715858	2
CCAACGA	140	6.8030204E-10	11.5357	19
GTCTTAG	315	0.0	11.474098	1
TCCAACG	200	0.0	11.400264	18
GGCGAGG	485	0.0	11.360809	19
TGGCGAG	1350	0.0	11.329892	18
GTCTTAT	245	0.0	11.258419	1
CTGTAGG	1760	0.0	11.240773	1
GACGTGG	595	0.0	11.015171	7
AAACCGT	70	0.0014926797	10.85633	6
AGGCGTG	150	2.1391315E-9	10.765068	7
AGGACGT	1215	0.0	10.712709	5
AGACCCG	80	3.7692412E-4	10.688272	5
TAGGACA	580	0.0	10.648366	4
>>END_MODULE