FastQCFastQC Report
Thu 26 May 2016
SRR1513071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038995
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC44080.21618493424456656No Hit
CCCATGTACTCTGCGTTGATACCAC37840.18558162231883843No Hit
GTATCAACGCAGAGTACTTTTTTTT34250.16797490920772243No Hit
GTCCTACAGTGGACATTTCTAAATT27140.1331047893692726No Hit
TATCAACGCAGAGTACTTTTTTTTT22270.10922047381185337No Hit
CTGTAGGACGTGGAATATGGCAAGA22080.10828864219873027No Hit
GTCCTACAGTGTGCATTTCTCATTT21030.10313904644199716No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC350.002189984516.2639528
GCCGGTT1101.9099389E-1013.79768611
CCGGTTT1155.3896656E-912.38173112
TAGGACC7650.012.3196974
TCCAACG1800.012.12683218
GTCCTAC16550.011.8610551
CCAACGT1201.0239091E-811.857676519
TGCCGGT1201.0253643E-811.85651310
GACGTGG5600.011.5205817
CTAGGAC3100.011.3623023
GTAGGAC14600.011.3454753
TGTAGGA16000.011.2535342
CCTACAG18650.011.2297513
TAACGTG600.005786492511.1063945
TCCTACA18950.011.0601382
CTGTAGG16150.010.9157441
AGGACGT11000.010.8179165
GGACGTG11200.010.7097376
TAGGACG11700.010.6588844
TACACTG10650.010.6371095
Produced by FastQC (version 0.11.5)