FastQCFastQC Report
Thu 26 May 2016
SRR1513070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208281
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC48980.22180148269174077No Hit
CCCATGTACTCTGCGTTGATACCAC39610.17937028847325137No Hit
GTCCTACAGTGGACATTTCTAAATT23880.1081384117329271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT400.005276400614.2514794
CGTGCGC658.034038E-411.69087210
GCGTTTA1406.82121E-1011.53377513
GTCCTAA6700.011.4974681
GTAGGAC12550.011.4299113
GGACGTG10150.011.2309116
TAGGACC5850.011.2062914
AGGACGT10500.011.1294745
CGCAGAT1803.6379788E-1211.0819728
GTCCTAC12550.011.0637181
GGCGAGG4650.011.03315319
CCAACGA1053.4643963E-610.85802319
ACCGTCT1053.4728691E-610.8558098
TAGGACG10600.010.8454654
GTCTAGG2200.010.8071041
GCTCTAA1605.347829E-1010.6990331
CCGCCCA803.7763105E-410.6861889
TTAGGAC8900.010.6738143
GACGTGA5300.010.394957
TAGGACA6650.010.286784
Produced by FastQC (version 0.11.5)