FastQCFastQC Report
Thu 26 May 2016
SRR1513070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208281
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC56870.25753063129194154No Hit
CCCATGTACTCTGCGTTGATACCAC53990.24448881279148807No Hit
GTATCAACGCAGAGTACTTTTTTTT25970.11760278696415899No Hit
GTCCTACAGTGGACATTTCTAAATT24180.10949693449339101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC1655.456968E-1211.5408573
CGTACTG752.0320373E-411.425455
TAGGACC5850.010.9046324
TCCAACG1408.507413E-910.84465118
GTCCTAC13600.010.7897071
TGGCGAG10450.010.7149318
GGACCGT1201.4813177E-610.3146426
GGCGAGG4900.010.26020119
TGCCGGT1303.9249426E-710.21506310
TAGGACT4200.010.2012954
TCCTACA15900.010.1859282
GACTTCG750.002677254410.12006957
ACTGCGT750.002677757510.119848
TTAGGAC7450.010.0963153
TCTATAC2850.010.0223253
ATTGGAC1553.9270162E-89.8283433
AGGACGT11400.09.7717675
GGACGTG11400.09.6882476
ATGGCGA12450.09.68224517
GTAGGAC13700.09.6602043
Produced by FastQC (version 0.11.5)