FastQCFastQC Report
Thu 26 May 2016
SRR1513069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770502
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC40860.14748229743201774No Hit
GTCCTACAGTGGACATTTCTAAATT35820.12929064840956622No Hit
CCCATGTACTCTGCGTTGATACCAC33460.12077233656571985No Hit
CTGTAGGACGTGGAATATGGCAAGA28800.10395228012829445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTACG508.704584E-515.2027162
TAGGACC8600.012.8160124
AGTGCGA550.003074515812.08826711
GGCGAGG6600.011.6592819
TATACGA752.0749684E-411.39997818
TGGCGAG15800.011.36390418
TCTACGG600.005869813711.0853142
TGTACCG1201.2743658E-711.0847145
GTCCTAC19050.011.0355221
GTCTTAG3500.010.8714521
GTAGGAC19700.010.8031813
TATGGCG17350.010.5669416
GTCCTAA9500.010.5138391
AGGACGT17000.010.507195
TGTAGGA22050.010.3850762
GGACGTG16450.010.2207586
CGGACAG750.002645955210.1345965
AGGACCT15550.010.0816415
ATGGCGA18100.010.02411817
CTAATAC2002.5465852E-119.9760633
Produced by FastQC (version 0.11.5)