FastQCFastQC Report
Thu 26 May 2016
SRR1513069_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513069_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770502
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC46400.16747867354002993No Hit
CCCATGTACTCTGCGTTGATACCAC44440.16040414336463213No Hit
GTCCTACAGTGGACATTTCTAAATT36520.13181726632935115No Hit
CTGTAGGACGTGGAATATGGCAAGA32670.11792086777053401No Hit
GTATCAACGCAGAGTACTTTTTTTT27950.1008842440828413No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCGG551.987866E-413.7960510
ACCGACC655.5269044E-513.1356248
TAGGACC8950.012.6569744
CCGGTTT905.46097E-712.65688812
GTAGGAC19650.011.8204283
GTCGTAG657.8195665E-411.7288341
GTCCTAC19750.011.6286191
GCGTGCG907.582443E-611.5950269
CGTGCGC1157.240487E-811.54669410
TGTAGGA21150.011.5322292
GGCGAGG7600.011.36026719
GCCGGTT1105.066395E-711.21111111
TCGAACT1950.011.19061619
CCTCGAC600.005789407511.1058723
AGGACGT17800.011.016745
GACGTGG8900.010.9797457
TCCTACA23200.010.9649582
CTGTAGG21450.010.929141
GGACGTG17300.010.8399186
TAGGACG18900.010.6273884
Produced by FastQC (version 0.11.5)