FastQCFastQC Report
Thu 26 May 2016
SRR1513068_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513068_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522264
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC88570.3511527738571379No Hit
CCCATGTACTCTGCGTTGATACCAC72890.28898640269218445No Hit
GAGTACATGGGAAGCAGTGGTATCA34240.13575105540102067No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGT400.005283271414.248795517
AAGACCG551.9590223E-413.8197545
CGATCGC550.00306993312.09060619
GTAGGAC11150.011.3331283
GTCCTAC10850.011.3090031
GATACGA600.005879339811.08283618
TTCGCGG600.005881075711.082396516
CGGTCCA600.00588454911.0815179
TAGGACC6200.011.034174
TCCTACA11850.010.984592
GTGCGCC1451.4482794E-810.48104111
TGGCGAG9600.010.39015918
GCGTGCG1106.0607235E-610.3619389
GGCGAGG4500.010.34418619
TGTAGGA13200.010.2930422
GTCCTAT2053.6379788E-1210.2078241
AGGACCT10150.010.2031325
AGGACGT8850.010.0915435
TAGGACG8950.010.08554654
GTCCTAG3600.010.0402711
Produced by FastQC (version 0.11.5)