FastQCFastQC Report
Thu 26 May 2016
SRR1513068_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513068_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522264
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC102530.40649987471573157No Hit
CCCATGTACTCTGCGTTGATACCAC95170.37731974131177387No Hit
GAGTACATGGGAAGCAGTGGTATCA36640.14526631629361558No Hit
GTATCAACGCAGAGTACTTTTTTTT33240.13178636336243946No Hit
CATGTACTCTGCGTTGATACCACTG26630.10557974898741766No Hit
GTATCAACGCAGAGTACATGGGAAG26360.10450928213700073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACCG402.7192713E-416.6589895
GTACTAG1501.8189894E-1212.7034351
GTAGGAC10700.011.74358753
GTCCTAC12250.011.1997621
TAGGACC5450.011.1787724
TATGCGA600.005905461511.07623612
CGTGTAG600.005951851611.06459710
TGTAGGA12000.011.034352
GGACCGG1158.5938154E-710.7610866
GGACCGT803.7018908E-410.7093516
AGTCCGG803.8318627E-410.66943310
TGTACCG1355.523907E-810.5771375
CCAACGA909.6550655E-510.54084719
ATTAGAC1553.5452103E-910.4406573
GGACGTG7500.010.4079036
GGTCTAC2400.010.321541
GACGTGG4050.010.3061977
TAGGACG8350.010.2604564
TGGCGAG8700.010.25474318
CGTTTCC750.002662656410.12684414
Produced by FastQC (version 0.11.5)