FastQCFastQC Report
Thu 26 May 2016
SRR1513067_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513067_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173556
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95670.44015429094074415No Hit
CCCATGTACTCTGCGTTGATACCAC77910.3584448709856107No Hit
GAGTACATGGGAAGCAGTGGTATCA40670.18711273139500434No Hit
GCGTTGATACCACTGCTTCCCATGT27140.12486450774675234No Hit
GTATCAACGCAGAGTACATGGGAAG27030.12435842462766085No Hit
CATGTACTCTGCGTTGATACCACTG26560.1221960694824518No Hit
GTATCAACGCAGAGTACTTTTTTTT25690.11819341208600101No Hit
GCTTCCCATGTACTCTGCGTTGATA21810.10034248024895609No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCG551.961846E-413.8172457
TAGGACC3400.012.575574
GTTCTAG2350.011.7374651
GCGTTTA1751.8189894E-1211.39791613
GTAGGAC4100.011.3544563
CAACCGT600.005879902311.08258156
TGTAGGA5550.010.9559762
ACGGCGT700.00149266110.8564068
CAGGACT5200.010.7805964
GTCCTAC4150.010.7719391
TACTGCG803.7743413E-410.6867757
CGTTTAT1909.094947E-1210.4980814
GTCCTAA2400.010.3040091
CTTATAC2600.010.2314883
TGCGTTT1951.4551915E-1110.22889912
GTGTAGC5500.010.2031311
ACAACCG750.002645237610.1347465
GTATAAG2850.010.0119931
TAGACTG2800.09.8406565
TAGGACG2054.1836756E-119.7332874
Produced by FastQC (version 0.11.5)