##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513067_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2173556
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.13587641634262	31.0	30.0	34.0	23.0	34.0
2	28.819676143609826	31.0	30.0	34.0	16.0	34.0
3	29.163411938776825	31.0	30.0	34.0	22.0	34.0
4	31.995982160109975	35.0	32.0	37.0	18.0	37.0
5	32.20127339714275	35.0	33.0	37.0	19.0	37.0
6	32.671829481274	36.0	33.0	37.0	25.0	37.0
7	32.64989767919483	37.0	33.0	37.0	25.0	37.0
8	32.64328041237493	37.0	33.0	37.0	25.0	37.0
9	34.002736529447596	39.0	35.0	39.0	25.0	39.0
10	33.48823126710331	38.0	34.0	39.0	17.0	39.0
11	33.886271621251076	39.0	34.0	39.0	23.0	39.0
12	33.713483342504176	39.0	34.0	39.0	21.0	39.0
13	33.80383942258677	39.0	34.0	39.0	22.0	39.0
14	34.10550084745919	39.0	33.0	41.0	11.0	41.0
15	34.650299785236726	40.0	34.0	41.0	18.0	41.0
16	34.229199523729775	39.0	34.0	41.0	16.0	41.0
17	33.76631335930613	39.0	33.0	41.0	10.0	41.0
18	33.16801131417824	39.0	32.0	41.0	10.0	41.0
19	34.05982960641456	39.0	33.0	41.0	17.0	41.0
20	34.32893562438695	39.0	34.0	41.0	17.0	41.0
21	34.35564991194154	39.0	34.0	41.0	13.0	41.0
22	33.93971629900495	39.0	34.0	41.0	8.0	41.0
23	32.25534653811542	39.0	31.0	41.0	2.0	41.0
24	33.34314183761541	39.0	32.0	41.0	2.0	41.0
25	33.08964020250686	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.968206449829097
1101	2	1.3085081714437408
1101	3	1.0779580246361427
1101	4	0.9708302518204306
1101	5	1.2806323302926685
1101	6	1.044123548211772
1101	7	0.9882310132013714
1101	8	0.9674843598555327
1101	9	1.165968248606987
1101	10	1.4804962112738167
1101	11	1.1322256162096096
1101	12	1.1585419159341939
1101	13	1.1041947965709795
1101	14	0.2875257576393935
1101	15	0.918513032717641
1101	16	1.5364587479174503
1101	17	2.1752180409326556
1101	18	-4.313999515806529
1101	19	-0.47458387823071035
1101	20	0.538028968080333
1101	21	0.9019299445961266
1101	22	1.5413678975736858
1101	23	3.4177321443919624
1101	24	2.3667978903595994
1101	25	2.498242694269976
1102	1	1.0784609007907449
1102	2	1.2622999948903768
1102	3	1.039638425871761
1102	4	1.7058630157122536
1102	5	1.4842913118075813
1102	6	1.0046556804421058
1102	7	1.0150949823063513
1102	8	0.8992840311947532
1102	9	0.9295529446109043
1102	10	1.4515077174288251
1102	11	1.0226541090251686
1102	12	1.074090969425967
1102	13	1.0174946204324158
1102	14	1.8450249306889006
1102	15	1.355668391167562
1102	16	1.6673059690988907
1102	17	1.4289752724916553
1102	18	1.8498534376367246
1102	19	1.5087732228328576
1102	20	1.4306359171893845
1102	21	1.501809667770189
1102	22	1.8793777589109268
1102	23	3.511247376554998
1102	24	2.2922385584598928
1102	25	2.6614806387700582
1103	1	0.9744130266047222
1103	2	1.1454436861613928
1103	3	0.9220143493084976
1103	4	1.4188779866171473
1103	5	1.2925146050432161
1103	6	0.9203974196974727
1103	7	0.8262442546589241
1103	8	0.7916153820743119
1103	9	0.8416486384668929
1103	10	1.2122358816175662
1103	11	0.9592593145138011
1103	12	1.1350879091442678
1103	13	0.9586388557282746
1103	14	1.7640833474857587
1103	15	1.19931008663972
1103	16	1.5650852347107786
1103	17	1.9451898249857962
1103	18	2.0828369028023843
1103	19	1.4682649514405597
1103	20	1.4337799605432835
1103	21	1.3516148012521967
1103	22	1.6975672692261057
1103	23	3.1518479845226324
1103	24	2.1185029761119694
1103	25	2.1419846170226933
1104	1	0.8138570755381949
1104	2	1.2470640496241359
1104	3	0.9363232258536556
1104	4	1.4288312215890286
1104	5	1.2014076337715238
1104	6	0.9451011772411917
1104	7	0.7797989509846559
1104	8	0.8310094118953657
1104	9	0.8834654690456176
1104	10	1.2641062695925172
1104	11	0.9805288052709216
1104	12	1.0303314041729053
1104	13	0.8253674778864593
1104	14	1.8121835448623074
1104	15	1.2641740758628757
1104	16	1.4663534749900933
1104	17	1.95672235659233
1104	18	2.564542554535784
1104	19	1.7864771660509717
1104	20	1.0621072617421774
1104	21	1.2172577813113392
1104	22	1.620599791596156
1104	23	3.0422745821856623
1104	24	2.067696346874378
1104	25	2.1030950413183405
1105	1	0.6432991145114677
1105	2	1.0565433076561668
1105	3	0.667647565354585
1105	4	0.993416034171112
1105	5	1.09445156803951
1105	6	0.731160737016765
1105	7	0.48017114157222096
1105	8	0.5015158602597225
1105	9	0.7498717879322072
1105	10	1.2049386399639204
1105	11	0.8423045534807372
1105	12	0.8114267211614106
1105	13	0.82942212771637
1105	14	1.5188147559667726
1105	15	0.944724565233166
1105	16	1.3342968203430488
1105	17	1.7896528701874956
1105	18	1.5167940291437603
1105	19	1.0660572682409537
1105	20	0.983723552737672
1105	21	0.9364775733115636
1105	22	1.421850945990279
1105	23	2.9241713619481473
1105	24	1.8847878769860813
1105	25	1.9422808628060793
1106	1	-0.4493645633139707
1106	2	-0.12023437443192364
1106	3	-0.29752781907334835
1106	4	-2.5163513291226316
1106	5	-0.4259036591558072
1106	6	-0.45203556929977395
1106	7	-0.36882102448846155
1106	8	-0.2879541511768906
1106	9	-0.3218758165180091
1106	10	-0.23808636642821313
1106	11	-0.4418771104675372
1106	12	-0.3606212442943004
1106	13	-0.4582556866450176
1106	14	0.31355336158649294
1106	15	-0.17959895329201458
1106	16	-1.2023848828349273
1106	17	-5.850915895775525
1106	18	-7.045828009821946
1106	19	-3.016931536065634
1106	20	-1.7209669341997653
1106	21	-1.2011900413291485
1106	22	-1.5820914075883437
1106	23	-5.381043418324026
1106	24	-2.2817227939524685
1106	25	-0.9261375712737276
1107	1	0.6168076738355452
1107	2	0.9291760644738432
1107	3	0.5872167101723313
1107	4	0.9032530279189501
1107	5	0.890074788102865
1107	6	0.4641333127084408
1107	7	0.5022437402149791
1107	8	0.4876410838541432
1107	9	0.4375676870117502
1107	10	0.804051227961665
1107	11	0.5532219447734406
1107	12	0.6044558440282799
1107	13	0.5668818628591197
1107	14	1.4225536387581954
1107	15	0.8062299774908297
1107	16	-5.9353597082569465
1107	17	-0.9887106909985661
1107	18	0.6115901172794764
1107	19	0.7241844065091598
1107	20	0.5702651107141534
1107	21	0.6235998739921413
1107	22	0.7491467461211982
1107	23	2.6236038362085523
1107	24	1.6835689692253126
1107	25	1.3519072781599064
1108	1	0.25027194600561486
1108	2	0.5266941467932185
1108	3	0.3337004836453552
1108	4	0.9077758545392172
1108	5	0.644669378080053
1108	6	0.32408113767810676
1108	7	0.2426556129440698
1108	8	0.2049200629476502
1108	9	0.2929828974265405
1108	10	0.7964274056948923
1108	11	0.28951576646895205
1108	12	0.4077245870777304
1108	13	0.21405556114834923
1108	14	0.8112618858761351
1108	15	0.4125714379538934
1108	16	0.7383900121195595
1108	17	0.9977819592413368
1108	18	0.5242426778120688
1108	19	0.6718993063972718
1108	20	0.5618710669749021
1108	21	0.6022360160652624
1108	22	0.9762698069756368
1108	23	2.656582769191985
1108	24	1.4797394131152615
1108	25	1.5424647508653635
1109	1	0.1053417836316406
1109	2	0.45818633153032096
1109	3	0.29394715670597904
1109	4	0.35006726778414077
1109	5	0.12694366095348641
1109	6	0.15794941537883034
1109	7	0.06937741989268176
1109	8	0.05293608679649964
1109	9	0.1704389997327027
1109	10	0.5630225200900725
1109	11	0.223117438690565
1109	12	0.3166505542441058
1109	13	0.08838580847724842
1109	14	-1.091083023592553
1109	15	-0.24773978226073012
1109	16	-0.5527256046640616
1109	17	0.5969123239416945
1109	18	1.4275964492904833
1109	19	0.5155187255122016
1109	20	0.37119650454009445
1109	21	0.3127347035885961
1109	22	0.10167651033696501
1109	23	-4.83522383452512
1109	24	-1.8090552223943561
1109	25	-0.4589796078616999
1110	1	0.6881231731594717
1110	2	0.9328491282596687
1110	3	0.8006585897549421
1110	4	1.3302145206742608
1110	5	1.006749527103885
1110	6	0.7159852916609282
1110	7	0.6041649557915463
1110	8	0.6371697470539104
1110	9	0.6820452873888243
1110	10	1.0230636681958387
1110	11	0.7245043141625942
1110	12	0.7531606351645337
1110	13	0.5871417322302932
1110	14	1.484671443190706
1110	15	0.9846668702513242
1110	16	1.3729989578207835
1110	17	1.606737015050264
1110	18	2.219549464951669
1110	19	1.4544656608502606
1110	20	1.0939602934760515
1110	21	1.0691025178441294
1110	22	1.3685118732728156
1110	23	2.6829942922048815
1110	24	1.7642789672122134
1110	25	1.9903581248364262
1111	1	0.406067440159422
1111	2	0.7786807257229853
1111	3	0.4921860010712713
1111	4	1.0026111013960772
1111	5	0.7396901680642429
1111	6	0.3546249867650033
1111	7	0.1358503698544098
1111	8	-0.0017013973859079101
1111	9	0.13417153528649095
1111	10	0.4455289122205883
1111	11	0.2166203782894911
1111	12	0.3406461695810492
1111	13	0.18947937052126917
1111	14	-0.45131121353907133
1111	15	0.04834552599358233
1111	16	0.4621655859125582
1111	17	1.0695878536683594
1111	18	1.7674247600495718
1111	19	0.9266030183160439
1111	20	0.6190223990478074
1111	21	0.5587351305985351
1111	22	0.8099050439744175
1111	23	0.7321093842445734
1111	24	0.6769708772864291
1111	25	0.4847076750532153
1112	1	0.21022016300700486
1112	2	0.6454200692648833
1112	3	0.425840464783537
1112	4	1.0459569419125856
1112	5	0.7244499963439992
1112	6	0.15934560333532488
1112	7	0.11938145189476757
1112	8	0.08398970899276037
1112	9	0.24457953988372338
1112	10	0.5230227431369983
1112	11	0.21801137987824148
1112	12	0.3074581899981794
1112	13	0.11263118068251288
1112	14	0.5846722905004142
1112	15	0.20638392642626258
1112	16	0.7212823083545032
1112	17	0.8532541484998504
1112	18	1.084801293869674
1112	19	0.5597250816632382
1112	20	0.4467511621888036
1112	21	0.4802981904639978
1112	22	0.4102160333940148
1112	23	2.140178207555259
1112	24	1.0990545063977422
1112	25	1.105036918199616
1113	1	0.2792049071144831
1113	2	0.7054010653849261
1113	3	0.49022633592667475
1113	4	0.5190925161200965
1113	5	0.7155933964548566
1113	6	0.18636014658235212
1113	7	0.29759033620864983
1113	8	0.11223455551003525
1113	9	0.35626758591534724
1113	10	0.6268274782704211
1113	11	0.3767251207392235
1113	12	0.30436082150767163
1113	13	0.23058126910333954
1113	14	0.8506454475199732
1113	15	0.3895575743793813
1113	16	0.8080680132136919
1113	17	1.205523726455347
1113	18	1.861933291652548
1113	19	0.8629514521861026
1113	20	0.6860535614286434
1113	21	0.6213142381848513
1113	22	0.8341229803679084
1113	23	0.6949027832672385
1113	24	0.5680837908488741
1113	25	1.0772054720571376
1114	1	-0.4993124772107933
1114	2	-3.257609516378288
1114	3	-1.2231533997630102
1114	4	-0.0317620898299964
1114	5	0.22479984213565984
1114	6	0.012361050473757018
1114	7	-0.08489988462137177
1114	8	-0.13651967174073576
1114	9	0.10255267014867542
1114	10	0.16518442187071258
1114	11	-0.14169848485244074
1114	12	0.07170618594733469
1114	13	-0.13718370354614962
1114	14	0.7486907823493993
1114	15	0.1099356503374338
1114	16	0.4834696994387002
1114	17	0.8103059743397765
1114	18	0.9565302028374276
1114	19	0.3510187219665255
1114	20	0.11130330039104308
1114	21	-0.051771226306243534
1114	22	0.1887396446969305
1114	23	1.7798919521546779
1114	24	0.7834505292034777
1114	25	0.7922770820747473
1115	1	-0.05894252350251605
1115	2	0.23938449889012503
1115	3	0.10480045293962093
1115	4	0.0764695132013884
1115	5	0.13199437885104714
1115	6	-0.2769080496121319
1115	7	-0.4531876483536976
1115	8	-0.42661445955133814
1115	9	-0.5276741516852042
1115	10	-0.269795916183007
1115	11	-0.23343660343728345
1115	12	-0.33996929819008415
1115	13	-0.42609617748014017
1115	14	0.40174072774696157
1115	15	-0.24558673412963117
1115	16	0.19435895910054057
1115	17	0.7204751405567009
1115	18	1.0784570735727996
1115	19	0.3816949054205878
1115	20	-0.1569384756705432
1115	21	-0.118710525114615
1115	22	0.23958959313499406
1115	23	1.9846283021778603
1115	24	0.6242315214729928
1115	25	0.5230313052125055
1116	1	0.5364571496109178
1116	2	0.42887373129356376
1116	3	0.5192391411678869
1116	4	1.2987983745856369
1116	5	0.6281017437755949
1116	6	0.44737860995340384
1116	7	0.4361901521163887
1116	8	0.506099725975858
1116	9	0.5795232214848625
1116	10	1.06192051705613
1116	11	0.6253148747414201
1116	12	0.6886606392202381
1116	13	0.5995549547387284
1116	14	1.385701938577057
1116	15	0.7384629473466973
1116	16	1.0188121430167811
1116	17	1.2882111576463515
1116	18	1.8005673992562805
1116	19	1.0163321170840405
1116	20	0.7392995411784966
1116	21	0.7657711660098556
1116	22	0.9865421241584045
1116	23	2.3340810459416304
1116	24	1.3287067716996646
1116	25	1.5251371413408066
1201	1	0.8580128762449704
1201	2	1.2000626913194061
1201	3	0.9473543617156714
1201	4	1.5410119936911144
1201	5	1.273173248301653
1201	6	0.8616982080883773
1201	7	0.8010213870052496
1201	8	0.7847547866335702
1201	9	0.882192619941712
1201	10	1.1550302896009867
1201	11	0.8033856995994029
1201	12	0.9927546351028695
1201	13	0.8723536377075902
1201	14	1.7266321410099223
1201	15	1.1993921167496637
1201	16	1.588440981932166
1201	17	2.0304000168288
1201	18	2.6614874681577376
1201	19	1.7313161338158451
1201	20	1.3975853018451474
1201	21	1.3122721051652135
1201	22	1.5645903207423544
1201	23	3.2273556933381897
1201	24	2.1967256417125327
1201	25	2.1826396029854678
1202	1	1.119094165852161
1202	2	1.3802164966143877
1202	3	1.1886553676340945
1202	4	1.8324001378501364
1202	5	1.4370695198705548
1202	6	1.0580517780422554
1202	7	0.978890212451553
1202	8	0.9289593666737801
1202	9	1.1914732787417037
1202	10	1.6104275294802548
1202	11	1.2245588381462937
1202	12	1.2061787705538904
1202	13	1.1312075226773572
1202	14	2.06546749335277
1202	15	1.5002092194952468
1202	16	1.7708555350845572
1202	17	2.1803983690533926
1202	18	2.931787770379131
1202	19	2.0657269184089557
1202	20	1.8119529341081986
1202	21	1.6471485317025412
1202	22	2.130419335934846
1202	23	3.6361373669739763
1202	24	2.277859001022563
1202	25	2.4522214751297327
1203	1	0.9903242770649001
1203	2	1.3081714536749018
1203	3	1.0049025459582381
1203	4	1.6321744999614367
1203	5	1.5222720601723054
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2211	16	-0.6269980842891769
2211	17	-0.07619779330677545
2211	18	-5.601579149262481
2211	19	-1.9811332556830692
2211	20	-1.0739933511473225
2211	21	-0.9322579425522974
2211	22	-0.6395996753524855
2211	23	-4.745131979951932
2211	24	-2.184487334881833
2211	25	-1.5110935653399764
2212	1	0.04809689651636617
2212	2	0.3473864406275311
2212	3	0.040804460800842435
2212	4	0.6269234254160025
2212	5	0.43777333895443604
2212	6	-0.02303521311742429
2212	7	-0.19605965211409426
2212	8	-0.2843648461312256
2212	9	-0.5029258377757913
2212	10	-4.071536900459257
2212	11	-1.4842073082107134
2212	12	-0.6404903136022142
2212	13	-0.4625333773979108
2212	14	0.3133194990448587
2212	15	-0.07523348325794643
2212	16	0.1358002899566202
2212	17	0.6437887881032083
2212	18	1.400991702096043
2212	19	0.4422052956142224
2212	20	0.045837894576756355
2212	21	0.014856916544879084
2212	22	0.1517086270060659
2212	23	1.5871760632878562
2212	24	0.6847944485602895
2212	25	0.5186722160352986
2213	1	-1.3756245291719154
2213	2	-3.628734601141282
2213	3	-1.6241550062157621
2213	4	-3.3816939492941067
2213	5	-1.1108104547444704
2213	6	-0.8388553769417122
2213	7	-0.7632902559410724
2213	8	-0.5977201732867741
2213	9	-0.5745320043713207
2213	10	-0.593332484839479
2213	11	-0.6783365681489641
2213	12	-0.6747745402511782
2213	13	-0.9852831306242251
2213	14	-6.166973749643308
2213	15	-2.6918232602591488
2213	16	-1.7275606158367367
2213	17	-0.6328887867622797
2213	18	0.044972590740634644
2213	19	-0.642044247944014
2213	20	-0.7060031412005685
2213	21	-0.8871745265002957
2213	22	-0.7569552534125066
2213	23	-5.743617027936008
2213	24	-3.113897932385264
2213	25	-2.1762556250523417
2214	1	-0.20887457589766711
2214	2	-0.16912876976715197
2214	3	-0.17184413075547056
2214	4	0.13338223827782514
2214	5	-0.12590106991771677
2214	6	-0.3066744482632515
2214	7	-0.4130048189650992
2214	8	-0.48603086512304117
2214	9	-0.4878524512334792
2214	10	0.04337805485118196
2214	11	-0.43721052665649296
2214	12	-0.4106225389133513
2214	13	-0.39771955900593525
2214	14	0.002504823729239547
2214	15	-0.1854288817772627
2214	16	0.20386942177339762
2214	17	0.3835585147844256
2214	18	0.8952124496124583
2214	19	0.009316865079846082
2214	20	-0.1640780073353838
2214	21	-0.2834048238072526
2214	22	0.16731410334600838
2214	23	1.6535089283024575
2214	24	0.6154533829857058
2214	25	0.44375217896836716
2215	1	-2.7129252229666356
2215	2	-4.499981400217841
2215	3	-2.606381194114018
2215	4	-3.9762112523528366
2215	5	-4.989080973886498
2215	6	-2.602885782183769
2215	7	-1.8927253311523025
2215	8	-2.2912903684387764
2215	9	-1.9638038701871423
2215	10	-3.353014405564295
2215	11	-2.352135520714736
2215	12	-1.9846782656018576
2215	13	-2.020110661749399
2215	14	-1.3009184825593891
2215	15	-1.976335586098351
2215	16	-2.8796613487598357
2215	17	-7.1674459139603215
2215	18	-3.4211259058645958
2215	19	-2.5674875260726076
2215	20	-2.3194756079530165
2215	21	-2.3171792452044144
2215	22	-1.9804750971032377
2215	23	-6.908884333667661
2215	24	-4.078453714345709
2215	25	-3.731462229131683
2216	1	-1.234236072401675
2216	2	-0.690320701531256
2216	3	-0.9070160627636241
2216	4	-3.232368942305694
2216	5	-1.4752369535554983
2216	6	-1.1715449196424856
2216	7	-1.254855742077691
2216	8	-1.1124768511390783
2216	9	-1.1346505378950198
2216	10	-0.7812763117825412
2216	11	-1.2866001009142778
2216	12	-1.9993365265401017
2216	13	-1.4496817071188346
2216	14	-7.2311247231951405
2216	15	-3.5330067026702316
2216	16	-1.9018297155159587
2216	17	-1.0260530213774288
2216	18	-0.2715137038206876
2216	19	-0.8692996252619452
2216	20	-1.324124254483067
2216	21	-1.5148830852073587
2216	22	-1.0304729889408435
2216	23	0.14631530040285412
2216	24	-0.6187887251032791
2216	25	-0.5072447582258093
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	113675.0
3	2.0
4	954.0
5	5569.0
6	6753.0
7	6098.0
8	5682.0
9	5325.0
10	4812.0
11	4833.0
12	4775.0
13	4756.0
14	4729.0
15	4874.0
16	4984.0
17	5407.0
18	6027.0
19	7001.0
20	8632.0
21	10824.0
22	13283.0
23	16224.0
24	18994.0
25	21907.0
26	25348.0
27	28880.0
28	32536.0
29	39002.0
30	46056.0
31	52467.0
32	67735.0
33	87608.0
34	114908.0
35	151743.0
36	212598.0
37	295007.0
38	733548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.54664112082386	17.006162320495932	12.070042279598022	25.37715427908219
2	14.64830210842902	18.62783185225623	40.89623192995409	25.82763410936066
3	20.352371449453326	22.632738760399196	27.849642206884873	29.16524758326261
4	11.374149334263599	15.231421119751554	37.353401535397865	36.041028010586984
5	13.365397006572286	37.051906331118026	35.30615438428543	14.276542278024266
6	34.98010729086427	34.29120202028569	16.926058758728082	13.80263193012197
7	30.20803662188174	30.38552279733306	20.02883402580705	19.37760655497815
8	27.027173787993835	35.46190837762592	18.94839051103288	18.562527323347368
9	26.74806838885982	15.456570791379553	18.57627982900484	39.21908099075579
10	14.976009941204222	28.555050568972856	33.36507461656177	23.103864873261152
11	36.12558482203049	22.317825665398278	21.62986741963198	19.926722092939247
12	23.916295657175727	24.220051768766176	29.94480299108427	21.918849582973827
13	29.60796503219447	20.360031455932017	24.525181541128998	25.506821970744507
14	21.67286294963317	20.54680989214832	27.143630897570585	30.636696260647923
15	25.37750392298524	27.63843191032125	22.260835952491615	24.723228214201896
16	23.72016463177813	27.17758843159885	25.7821574052088	23.320089531414222
17	23.332521869263477	26.977897651524806	26.67886777292689	23.010712706284828
18	22.944363787799656	26.348846252073617	27.9164432950553	22.79034666507143
19	24.211092635606647	26.481846167723727	26.75043387776498	22.556627318904646
20	24.33263247606018	26.148833001311978	26.66704401595359	22.85149050667425
21	24.10822393081444	25.73353416731901	26.73581725027716	23.42242465158939
22	23.80523919148666	26.027509483251393	26.07937856513643	24.087872760125517
23	22.257796259709803	25.728865086958503	28.135335972162974	23.878002681168724
24	23.40562727366452	26.716110431977846	26.22547756160066	23.65278473275697
25	23.71430504337309	25.56736624156481	26.2864082919944	24.4319204230677
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	211.0
1	211.0
2	331.0
3	451.0
4	451.0
5	451.0
6	842.5
7	1234.0
8	1234.0
9	1234.0
10	1984.0
11	2734.0
12	2734.0
13	2734.0
14	4655.0
15	6576.0
16	6576.0
17	6576.0
18	11800.5
19	17025.0
20	17025.0
21	17025.0
22	27041.5
23	37058.0
24	37058.0
25	37058.0
26	52327.5
27	67597.0
28	67597.0
29	67597.0
30	88250.0
31	108903.0
32	108903.0
33	108903.0
34	131671.5
35	154440.0
36	154440.0
37	154440.0
38	178928.5
39	203417.0
40	203417.0
41	203417.0
42	228152.0
43	252887.0
44	252887.0
45	252887.0
46	284437.5
47	315988.0
48	315988.0
49	315988.0
50	311606.0
51	307224.0
52	307224.0
53	307224.0
54	280783.5
55	254343.0
56	254343.0
57	254343.0
58	222349.5
59	190356.0
60	190356.0
61	190356.0
62	158460.5
63	126565.0
64	126565.0
65	126565.0
66	99079.0
67	71593.0
68	71593.0
69	71593.0
70	52552.5
71	33512.0
72	33512.0
73	33512.0
74	23484.0
75	13456.0
76	13456.0
77	13456.0
78	9316.5
79	5177.0
80	5177.0
81	5177.0
82	3573.5
83	1970.0
84	1970.0
85	1970.0
86	1294.0
87	618.0
88	618.0
89	618.0
90	385.0
91	152.0
92	152.0
93	152.0
94	100.5
95	49.0
96	49.0
97	49.0
98	34.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1056333492212761
2	0.047755843419723255
3	0.03643798457458653
4	0.03215928184044948
5	0.0366220147997107
6	0.01831100739985535
7	0.019737241644567704
8	0.02327982347820806
9	0.028938752900776424
10	0.03593190145549505
11	0.02111746833299901
12	0.01982925675712979
13	0.016424697592332567
14	0.022037619458619883
15	0.021439521226966317
16	0.02134750611440423
17	0.024337997272672062
18	0.019369181194319355
19	0.03261935740325991
20	0.02314180080936493
21	0.0283866622254039
22	0.03579387878665192
23	0.028662707563090164
24	0.039198437951449144
25	0.030595024926893994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2173556.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.14930076209993
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.22312587284755	57.678947864543815
2	11.399702129571297	15.993622665712959
3	3.2113152105408833	6.7581454963841585
4	1.306434784922068	3.6658194661426986
5	0.6234052413324844	2.1865720885450965
6	0.3422174216405685	1.4403787702016764
7	0.2152861926059393	1.0571523112528987
8	0.14246618622404422	0.7995122676687851
9	0.09702709199244769	0.6125744392425134
>10	0.408099076021062	4.803935703309385
>50	0.019617036822237116	0.9092005245413297
>100	0.008513913303071762	1.0965828324607443
>500	0.0017934699705370101	0.8705632009048434
>1k	8.635225784063839E-4	1.3181695868110168
>5k	1.3284962744713598E-4	0.8088227822781161
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	9567	0.44015429094074415	No Hit
CCCATGTACTCTGCGTTGATACCAC	7791	0.3584448709856107	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4067	0.18711273139500434	No Hit
GCGTTGATACCACTGCTTCCCATGT	2714	0.12486450774675234	No Hit
GTATCAACGCAGAGTACATGGGAAG	2703	0.12435842462766085	No Hit
CATGTACTCTGCGTTGATACCACTG	2656	0.1221960694824518	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2569	0.11819341208600101	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2181	0.10034248024895609	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.60075562810436E-5	4.60075562810436E-5
2	0.0	0.0	0.0	4.60075562810436E-5	4.60075562810436E-5
3	0.0	0.0	0.0	4.60075562810436E-5	4.60075562810436E-5
4	0.0	0.0	0.0	9.20151125620872E-5	4.60075562810436E-5
5	0.0	4.60075562810436E-5	0.0	9.20151125620872E-5	4.60075562810436E-5
6	0.0	4.60075562810436E-5	0.0	9.20151125620872E-5	9.20151125620872E-5
7	0.0	4.60075562810436E-5	0.0	9.20151125620872E-5	9.20151125620872E-5
8	0.0	4.60075562810436E-5	0.0	1.380226688431308E-4	9.20151125620872E-5
9	0.0	4.60075562810436E-5	0.0	1.380226688431308E-4	9.20151125620872E-5
10	0.0	4.60075562810436E-5	0.0	1.380226688431308E-4	9.20151125620872E-5
11	0.0	4.60075562810436E-5	0.0	1.380226688431308E-4	9.20151125620872E-5
12	0.0	4.60075562810436E-5	0.0	1.840302251241744E-4	9.20151125620872E-5
13	0.0	4.60075562810436E-5	0.0	1.840302251241744E-4	9.20151125620872E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCG	55	1.961846E-4	13.817245	7
TAGGACC	340	0.0	12.57557	4
GTTCTAG	235	0.0	11.737465	1
GCGTTTA	175	1.8189894E-12	11.397916	13
GTAGGAC	410	0.0	11.354456	3
CAACCGT	60	0.0058799023	11.0825815	6
TGTAGGA	555	0.0	10.955976	2
ACGGCGT	70	0.001492661	10.856406	8
CAGGACT	520	0.0	10.780596	4
GTCCTAC	415	0.0	10.771939	1
TACTGCG	80	3.7743413E-4	10.686775	7
CGTTTAT	190	9.094947E-12	10.49808	14
GTCCTAA	240	0.0	10.304009	1
CTTATAC	260	0.0	10.231488	3
TGCGTTT	195	1.4551915E-11	10.228899	12
GTGTAGC	550	0.0	10.203131	1
ACAACCG	75	0.0026452376	10.134746	5
GTATAAG	285	0.0	10.011993	1
TAGACTG	280	0.0	9.840656	5
TAGGACG	205	4.1836756E-11	9.733287	4
>>END_MODULE