FastQCFastQC Report
Thu 26 May 2016
SRR1513067_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513067_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173556
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC111880.5147325396723158No Hit
CCCATGTACTCTGCGTTGATACCAC103680.47700634352186005No Hit
GAGTACATGGGAAGCAGTGGTATCA44750.20588381435767011No Hit
GTATCAACGCAGAGTACTTTTTTTT40060.18430627046186066No Hit
CATGTACTCTGCGTTGATACCACTG31050.14285346225264037No Hit
GTATCAACGCAGAGTACATGGGAAG28280.1301093691627913No Hit
TATCAACGCAGAGTACTTTTTTTTT27380.12596868909749737No Hit
GCGTTGATACCACTGCTTCCCATGT26910.12380633395228834No Hit
ACGCAGAGTACATGGGAAGCAGTGG26760.12311622060807267No Hit
GCTTCCCATGTACTCTGCGTTGATA25110.11552497382170047No Hit
TATCAACGCAGAGTACATGGGAAGC25030.11515691337145213No Hit
GGTATCAACGCAGAGTACTTTTTTT24560.11299455822624307No Hit
ACTCTGCGTTGATACCACTGCTTCC22830.10503525098962253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCG1551.8189894E-1212.2428649
GTCCTAA2700.011.6449431
GTGCGCC1552.5465852E-1111.63018511
TAGGACG2300.011.5876714
CGTGCGC1451.2478267E-911.12181510
AAGGCGT2400.011.1048526
CTAGGAC2950.010.9704093
CACCGCG1053.518644E-610.8439287
GGCGTGC2100.010.8436788
GGTCTAC2400.010.7186411
CCTAGAC2400.010.708253
GTTCTAG2050.010.6895931
ACCGCGC803.815471E-410.6742478
CTGATCG803.815471E-410.6742479
TCGAACT1605.5661076E-1010.67424719
GGACCGC1701.3460522E-1010.638266
TGTACCG1355.5304554E-810.576055
TCTACAC4050.010.5760493
TAGGACT2600.010.2506314
GTACCGT1409.4214556E-810.1983336
Produced by FastQC (version 0.11.5)