FastQCFastQC Report
Thu 26 May 2016
SRR1513066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990949
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC100720.5058894024909729No Hit
CCCATGTACTCTGCGTTGATACCAC82720.41548025589806675No Hit
GAGTACATGGGAAGCAGTGGTATCA41640.20914649245158964No Hit
GTATCAACGCAGAGTACATGGGAAG30270.1520380481870706No Hit
GTATCAACGCAGAGTACTTTTTTTT29360.14746736355376255No Hit
GCGTTGATACCACTGCTTCCCATGT27420.1377232666431938No Hit
CATGTACTCTGCGTTGATACCACTG24960.12536734994216325No Hit
TATCAACGCAGAGTACATGGGAAGC22960.11532188920961814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT1800.013.72006314
GCGTTTA1800.013.192368513
TAGGACC2700.012.316594
TTGCGCG701.0915203E-412.21389918
TACACCG1201.000808E-811.8758175
GTCTTAG2550.011.5628881
GATATAC2650.011.4854741
TATCGAC600.00587802711.08298219
GACCGTG1303.2736352E-810.9600897
GTAGGAC3650.010.9330833
ACCGTCC700.00149336710.8557078
ATTTGCG803.775411E-410.68635616
ACACCGT1701.382432E-1010.6165096
ATAGTAC2300.010.3275763
GTATTAG1752.3101165E-1010.3266721
TTTACAC2600.010.23224453
TGCGTTT2700.010.20209912
GTGTACG750.002621895410.1455011
CTAGTAC750.002644965410.1347933
TAATACT2350.010.107844
Produced by FastQC (version 0.11.5)