FastQCFastQC Report
Thu 26 May 2016
SRR1513066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1990949
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC111900.5620435279859002No Hit
CCCATGTACTCTGCGTTGATACCAC110690.5559660242427104No Hit
GTATCAACGCAGAGTACTTTTTTTT44970.22587218457127733No Hit
GAGTACATGGGAAGCAGTGGTATCA43400.21798649789622937No Hit
TATCAACGCAGAGTACTTTTTTTTT33090.1662021478199592No Hit
CATGTACTCTGCGTTGATACCACTG30230.15183713897241968No Hit
GTATCAACGCAGAGTACATGGGAAG29980.15058145638085155No Hit
GCGTTGATACCACTGCTTCCCATGT29330.14731668164277437No Hit
GGTATCAACGCAGAGTACTTTTTTT28440.14284645161679177No Hit
TATCAACGCAGAGTACATGGGAAGC27550.13837622159080923No Hit
ACGCAGAGTACATGGGAAGCAGTGG25200.12657280523006867No Hit
ACTCTGCGTTGATACCACTGCTTCC21030.10562801960271206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCGT456.663665E-414.806796
CGCCCTA400.00530460214.23993316
TGCGCAT655.5318385E-513.13398910
TTGCGCG604.1243824E-412.65517318
ATCGTCC600.005936427511.0682698
TAGGACC3100.011.0539174
GTCCTAC3450.011.0466971
GCGTGCG1553.2014214E-1011.0172639
ACCGTGG1252.2831045E-710.6255398
ACTGCGC909.649352E-510.5412098
AGGCGTG1553.745299E-910.4051937
GTAGGAC4900.010.2956833
CGTGCGC1303.9227234E-710.21532410
TAGACCG750.00260538610.1532285
AAGGCGT1502.3719622E-810.1532286
ATAAGAC2002.3646862E-119.9945833
TGTAGGA5200.09.8917332
TAGGACG2700.09.8711944
GTGCGCC1356.5669155E-79.83821511
CTAAGGT1752.4883775E-99.7906124
Produced by FastQC (version 0.11.5)