##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513066_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1990949
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.13185621530235	31.0	30.0	34.0	26.0	34.0
2	30.288644259596804	31.0	31.0	34.0	26.0	34.0
3	30.305445292671987	31.0	31.0	34.0	26.0	34.0
4	33.48660764288789	37.0	35.0	37.0	28.0	37.0
5	33.391406811525556	37.0	35.0	37.0	28.0	37.0
6	33.338287419717936	37.0	35.0	37.0	28.0	37.0
7	33.23562080193918	37.0	35.0	37.0	26.0	37.0
8	33.22553616390977	37.0	35.0	37.0	26.0	37.0
9	34.74711255788069	39.0	35.0	39.0	27.0	39.0
10	34.63314379223174	39.0	35.0	39.0	27.0	39.0
11	34.644347996859786	39.0	35.0	39.0	27.0	39.0
12	34.55098146662722	39.0	35.0	39.0	27.0	39.0
13	34.514606853314675	39.0	35.0	39.0	26.0	39.0
14	35.65791640067124	40.0	36.0	41.0	26.0	41.0
15	35.62380352284262	40.0	36.0	41.0	26.0	41.0
16	35.57797713552683	40.0	36.0	41.0	26.0	41.0
17	35.486594081515904	40.0	36.0	41.0	25.0	41.0
18	35.41253643363039	40.0	36.0	41.0	25.0	41.0
19	35.47333608244109	40.0	36.0	41.0	25.0	41.0
20	35.373307402650696	40.0	36.0	41.0	24.0	41.0
21	35.39073225883737	40.0	36.0	41.0	25.0	41.0
22	35.359232707618325	40.0	36.0	41.0	25.0	41.0
23	35.28409868861533	40.0	36.0	41.0	24.0	41.0
24	35.14610871498969	40.0	36.0	41.0	23.0	41.0
25	35.123738478484384	40.0	36.0	41.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.0377228385169381
1101	2	0.934639329123474
1101	3	1.0273978831739008
1101	4	1.2841175617931953
1101	5	1.1546764499077327
1101	6	1.112358582909465
1101	7	1.0231222644516649
1101	8	0.9800166566126052
1101	9	1.1568796570178677
1101	10	1.2041918350777792
1101	11	1.1598998009932444
1101	12	1.195880941808042
1101	13	1.141064242872318
1101	14	1.2340353974550524
1101	15	1.2785283318065197
1101	16	1.29091033037421
1101	17	1.1918054099429511
1101	18	1.1625718916752348
1101	19	1.1904372936593575
1101	20	1.4980084559060032
1101	21	1.4937370581627007
1101	22	1.4134279481449639
1101	23	1.3887626513285127
1101	24	1.3435711328272504
1101	25	1.3451741607636052
1102	1	0.8888095354933476
1102	2	0.8243152634349649
1102	3	0.7796740205219059
1102	4	1.211869136575352
1102	5	0.9151844543797267
1102	6	0.7427457407778064
1102	7	0.7265286752359543
1102	8	0.5884997970388284
1102	9	0.7427498337595679
1102	10	0.7462142920886663
1102	11	0.7580682444296087
1102	12	0.7489651894703115
1102	13	0.8383558241597058
1102	14	0.8442036544783349
1102	15	0.8048181013317262
1102	16	0.864657891509772
1102	17	0.9183214695739963
1102	18	0.794495035675979
1102	19	0.8389399594144606
1102	20	1.0547302569638504
1102	21	1.0484865319541257
1102	22	1.0536318686811015
1102	23	1.0548297533113669
1102	24	1.1389240661187543
1102	25	1.0831433824265346
1103	1	0.44790182340737417
1103	2	0.5879499637291588
1103	3	0.8198296393136459
1103	4	0.06322911577481705
1103	5	0.32614228996885686
1103	6	0.4670640659498204
1103	7	0.5970641444757234
1103	8	0.5454354742839129
1103	9	0.810375154554805
1103	10	0.7693856990616581
1103	11	0.77797656801269
1103	12	0.773631153156046
1103	13	0.7912171009849303
1103	14	0.9896383711433145
1103	15	0.8977247348716233
1103	16	0.8851208959346977
1103	17	0.9077459400512069
1103	18	0.9536605106455198
1103	19	0.9165822769403533
1103	20	1.024627265442959
1103	21	1.0126138003917262
1103	22	0.9759336978760018
1103	23	0.9638389835472481
1103	24	0.9484730508891062
1103	25	0.9631161298782303
1104	1	0.7114148806210316
1104	2	0.5464305233703577
1104	3	0.7383771971787816
1104	4	0.4912098404315799
1104	5	0.4740771155006769
1104	6	0.602183382690022
1104	7	0.5502275785543915
1104	8	0.5207512453834866
1104	9	0.4547841794750127
1104	10	0.5462291517594267
1104	11	0.505219778051746
1104	12	0.46959580739599716
1104	13	0.5783163182579614
1104	14	0.47273753500090265
1104	15	0.45447959424593165
1104	16	0.6320274742369634
1104	17	0.5515164596880524
1104	18	0.4428042039035418
1104	19	0.5901685323844319
1104	20	0.7361821698388553
1104	21	0.7652739847131471
1104	22	0.7546697740921999
1104	23	0.7323128090275617
1104	24	0.7553451380783684
1104	25	0.7128544896045099
1105	1	0.5766284517056768
1105	2	0.5277889859323928
1105	3	0.529523327390784
1105	4	0.47471537993926916
1105	5	0.5956227007450963
1105	6	0.5552820536696288
1105	7	0.4860437268878499
1105	8	0.47353635575568376
1105	9	0.47894215198985535
1105	10	0.5078308374975649
1105	11	0.5255331344097769
1105	12	0.46835329039318196
1105	13	0.46843545237679507
1105	14	0.49732859182874734
1105	15	0.4647972133639797
1105	16	0.4075725458320463
1105	17	0.4504504670947398
1105	18	0.44478825959250656
1105	19	0.42583686469210846
1105	20	0.6025979754116122
1105	21	0.48639208359026753
1105	22	0.5094963947447653
1105	23	0.538242243896029
1105	24	0.3663612900429243
1105	25	0.46877478998725053
1106	1	-0.2050721140390337
1106	2	-0.013528848716887865
1106	3	0.07933668258377935
1106	4	-1.2309078923583598
1106	5	-0.36578371743964055
1106	6	-0.014747291630037296
1106	7	0.08062397690392231
1106	8	0.19291713348722084
1106	9	0.27076769524235544
1106	10	0.4253646111153344
1106	11	0.4708787302201145
1106	12	0.38090395392057275
1106	13	0.3383450809132711
1106	14	0.32310769054890187
1106	15	0.46757422629526246
1106	16	0.43503327008983916
1106	17	0.3475324043844523
1106	18	0.2519452383413494
1106	19	0.20859749814147222
1106	20	0.39465316342742085
1106	21	0.3831458206366136
1106	22	0.5421684427505369
1106	23	0.4145845972024418
1106	24	0.4650370783865867
1106	25	0.3163169994961663
1107	1	0.5677367563166946
1107	2	0.3498131361134966
1107	3	0.5453204746393006
1107	4	0.9358769383305585
1107	5	0.6486145214059604
1107	6	0.5294404753497588
1107	7	0.3821000062449045
1107	8	0.3162330125765038
1107	9	0.32864273177867176
1107	10	0.4828293821664005
1107	11	0.40297780725511245
1107	12	0.3986485978763028
1107	13	0.4455088834731029
1107	14	0.47272385599214317
1107	15	0.42358677096598285
1107	16	0.5022142433000667
1107	17	0.4930982852698236
1107	18	0.6045146346536541
1107	19	0.5516370133248927
1107	20	0.6575308676347049
1107	21	0.5846921433911518
1107	22	0.6285959719049075
1107	23	0.579672498000626
1107	24	0.673152659059717
1107	25	0.7023047883315812
1108	1	-0.39799828813638527
1108	2	-0.2538031275085899
1108	3	-0.11939250556898173
1108	4	-1.0042417532471788
1108	5	-0.7104242591351522
1108	6	-0.4485710879058189
1108	7	-0.47374692136421714
1108	8	-0.4683671194725534
1108	9	-0.47679508025014883
1108	10	-0.2641704305543229
1108	11	-0.2343836261064567
1108	12	-0.21133868925562638
1108	13	-0.16103585968157574
1108	14	-0.1307919023573234
1108	15	-0.1058832058014687
1108	16	0.00926172260113134
1108	17	-0.09122211028230964
1108	18	-0.3148729850038805
1108	19	-0.19270818242772236
1108	20	-0.01797021885208494
1108	21	-0.015956449363393688
1108	22	-0.075413807048335
1108	23	-0.09563568320484706
1108	24	-0.07458845156359928
1108	25	-0.10575626852617859
1109	1	-0.01184754596147286
1109	2	-0.13899258777600565
1109	3	0.367206667445636
1109	4	-0.41159632030915816
1109	5	-0.020366866129371886
1109	6	0.03435397865096235
1109	7	0.18366348589696457
1109	8	0.34617080034411174
1109	9	0.32819288598171426
1109	10	0.4023536379959083
1109	11	0.3876095614375217
1109	12	0.354994590740219
1109	13	0.42780085160156744
1109	14	0.5095741350975658
1109	15	0.47126796001538906
1109	16	0.45894967897344685
1109	17	0.2584444370590262
1109	18	0.3423521090843167
1109	19	0.32659852419989477
1109	20	0.4506640056808493
1109	21	0.47886506623956393
1109	22	0.4175700276364793
1109	23	0.4523798869643869
1109	24	0.5809720106976499
1109	25	0.4165391079246419
1110	1	0.48400013152076937
1110	2	0.29501580028884433
1110	3	0.38399605779912704
1110	4	0.8637424962862141
1110	5	0.6466505650387049
1110	6	0.46652468550460213
1110	7	0.4674072017843045
1110	8	0.391828338011166
1110	9	0.44466623090478663
1110	10	0.41306936968504004
1110	11	0.32074693186994807
1110	12	0.3749358771076601
1110	13	0.28599064156137644
1110	14	0.41275325775996663
1110	15	0.42917236391402014
1110	16	0.44315653002581
1110	17	0.4969781856418507
1110	18	0.4245372756864825
1110	19	0.36350553931750085
1110	20	0.5339149145289426
1110	21	0.58528881540078
1110	22	0.5849783657622964
1110	23	0.5964967913273753
1110	24	0.6633048313172978
1110	25	0.6259324427094342
1111	1	0.3436975095657644
1111	2	-0.24422091875032947
1111	3	0.15188696938510304
1111	4	0.5354145252965523
1111	5	0.3350700965100515
1111	6	0.3656188360612873
1111	7	0.22226904189046337
1111	8	0.163704763100867
1111	9	0.3005937405446417
1111	10	0.08681000464316924
1111	11	0.20129812447602546
1111	12	0.23271980165241501
1111	13	0.14863628952121388
1111	14	0.17262534567595367
1111	15	0.21845470916182563
1111	16	0.26528563795992
1111	17	0.22218070299498294
1111	18	0.16026878166876912
1111	19	0.14120130142455167
1111	20	0.1607418182620748
1111	21	0.15009740236992997
1111	22	0.1591818908595144
1111	23	0.2114620010515651
1111	24	0.25766370490305945
1111	25	0.2081092352826559
1112	1	0.14362819833449691
1112	2	-0.1066575070173883
1112	3	0.18633907049809295
1112	4	0.5848092440365704
1112	5	0.34305658779759085
1112	6	0.36787203254758083
1112	7	0.22801008962586167
1112	8	0.16625121432124956
1112	9	-0.016043939839484267
1112	10	0.10570002869083339
1112	11	0.06723332118831848
1112	12	0.041540392360097655
1112	13	0.0952274891269056
1112	14	0.17029781793353038
1112	15	0.19110729998890008
1112	16	0.11991763815684209
1112	17	0.10661643572575485
1112	18	0.14015592608286198
1112	19	0.055942381528446106
1112	20	0.17395562848614077
1112	21	0.14854652480562436
1112	22	0.12247775620035384
1112	23	-0.030411615655381752
1112	24	0.034119865840018804
1112	25	0.044270838992588324
1113	1	-0.02372359221442011
1113	2	-0.11533904641235893
1113	3	-0.129173113369216
1113	4	0.3507614909118928
1113	5	0.056370745987180726
1113	6	0.02243699127068055
1113	7	-0.08875341409351023
1113	8	-0.28094647736725875
1113	9	-0.29576129972704734
1113	10	-0.18755994990610958
1113	11	-0.24737094717409747
1113	12	-0.19013573997733602
1113	13	-0.19877103180629518
1113	14	-0.21680490585262646
1113	15	-0.2557557087326785
1113	16	-0.30506303110205835
1113	17	-0.22730995146427802
1113	18	-0.25503518413496806
1113	19	-0.4285280116650725
1113	20	-0.2863369183214033
1113	21	-0.341944197528818
1113	22	-0.29176005562120366
1113	23	-0.34741624571722696
1113	24	-0.2896946755947596
1113	25	-0.2622243296600786
1114	1	-0.018074194931543275
1114	2	-0.29713497132051003
1114	3	-0.08863447636260346
1114	4	0.26309686396737675
1114	5	0.006877610018229063
1114	6	-0.15343790313264805
1114	7	-0.4944394116284343
1114	8	-0.35826757632444384
1114	9	-0.31600173370711815
1114	10	-0.3568559984637645
1114	11	-0.3238837740786522
1114	12	-0.3094024094137495
1114	13	-0.3699126233435237
1114	14	-0.3131359778839027
1114	15	-0.5174754061969722
1114	16	-0.4192698640723549
1114	17	-0.3883717346681266
1114	18	-0.39845257934448597
1114	19	-0.5552392712874763
1114	20	-0.3574041223193376
1114	21	-0.4063981316602323
1114	22	-0.45558337730180654
1114	23	-0.45679817627171104
1114	24	-0.348423440029606
1114	25	-0.48950833376721903
1115	1	-0.2404584144855768
1115	2	-0.22443913496053725
1115	3	-0.14319193573440359
1115	4	0.3016088555680696
1115	5	-0.07719147674004034
1115	6	-0.12223125530745449
1115	7	-0.2232828381721248
1115	8	-0.2778612841676704
1115	9	-0.6380184834919405
1115	10	-0.42185104392950024
1115	11	-0.5442491480900316
1115	12	-0.589493625746826
1115	13	-0.5131472932820103
1115	14	-0.46032247387856273
1115	15	-0.5573926746309965
1115	16	-0.6144644745157422
1115	17	-0.6163331116827493
1115	18	-0.5721093104913137
1115	19	-0.7239408577557995
1115	20	-0.5255972331767893
1115	21	-0.5671823316809892
1115	22	-0.6397644751940419
1115	23	-0.6193543090084646
1115	24	-0.5858262060229862
1115	25	-0.580517532719746
1116	1	-1.276159339075388
1116	2	-1.5650045929637173
1116	3	-1.4226566386866502
1116	4	-1.0512060433124688
1116	5	-1.3835359092212833
1116	6	-1.57659963929229
1116	7	-1.699326104250975
1116	8	-2.0550452764564433
1116	9	-2.1753325380064723
1116	10	-2.161575198046691
1116	11	-1.9902592690704992
1116	12	-2.1653755883429042
1116	13	-2.1956399979178585
1116	14	-2.3350343062811802
1116	15	-2.5337998768191383
1116	16	-2.3922568972270994
1116	17	-2.4978428559841106
1116	18	-2.528701156762878
1116	19	-2.660751012438631
1116	20	-2.7503572131759952
1116	21	-2.7675358641816175
1116	22	-2.61911759136013
1116	23	-2.767493327847369
1116	24	-2.4063660487059693
1116	25	-2.620889430347475
1201	1	0.9413597673326066
1201	2	0.8028353696682444
1201	3	1.0307551272052677
1201	4	1.1952217673509011
1201	5	0.909862385059121
1201	6	0.9727275362301526
1201	7	0.8774085944271945
1201	8	1.0049959934221633
1201	9	0.9931994502566468
1201	10	0.9146448873120931
1201	11	1.0280573432971352
1201	12	1.0540815557464
1201	13	0.9809392992993864
1201	14	1.069947800334603
1201	15	1.0573783459176056
1201	16	1.2298153002305767
1201	17	1.2767529629996943
1201	18	1.2467175954739176
1201	19	1.2660588946472018
1201	20	1.3804815197233538
1201	21	1.2197913744423303
1201	22	1.3245450851630665
1201	23	1.2870823807984166
1201	24	1.2961180036951703
1201	25	1.2226578525632732
1202	1	0.9038010818865985
1202	2	0.8089875077889452
1202	3	0.8716424894152937
1202	4	1.0864809842024172
1202	5	0.9201545462688543
1202	6	0.795984838769499
1202	7	0.7327478207806948
1202	8	0.7634674459686437
1202	9	0.8207228064056764
1202	10	0.8810525851131743
1202	11	0.8906657874414137
1202	12	0.9468632769476031
1202	13	0.9856783649341523
1202	14	1.0851278187322748
1202	15	1.088125916624655
1202	16	1.048350533364676
1202	17	0.9739972380782049
1202	18	1.2012787225127752
1202	19	1.1370216316563955
1202	20	1.1656507688726734
1202	21	1.1490053584628939
1202	22	1.1006247308861035
1202	23	1.2283732295215373
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2211	4	0.7062900959208207
2211	5	0.45768841448645503
2211	6	0.30836980361662114
2211	7	0.2591898477351222
2211	8	0.17813296812312984
2211	9	0.08561635509416021
2211	10	0.08844344770163559
2211	11	0.1147250685009027
2211	12	0.22966639749510165
2211	13	0.0821619096518802
2211	14	0.07445319887381885
2211	15	-0.013821505887200658
2211	16	0.0280912079261455
2211	17	0.10772443542480659
2211	18	0.12976247744703073
2211	19	0.055039523418244585
2211	20	0.01908372148052706
2211	21	0.08042834914329688
2211	22	-0.07019663178411406
2211	23	-5.242390148652021E-5
2211	24	-3.3518919465535895
2211	25	-2.3132927137540733
2212	1	0.04161039389902399
2212	2	-0.2223075726351169
2212	3	-0.5192522944928335
2212	4	0.20914003794004543
2212	5	-0.0451474999264434
2212	6	-0.13629362734884864
2212	7	-0.22379700372187727
2212	8	-0.22657750208223604
2212	9	-0.3185493763987495
2212	10	-0.40300438830629304
2212	11	-0.43973945100681533
2212	12	-0.3488087081795328
2212	13	-0.3250220951362124
2212	14	-0.4020575743278414
2212	15	-0.4015866276468216
2212	16	-0.49970916988405634
2212	17	-0.3909359281707552
2212	18	-0.5748582160778284
2212	19	-0.4630654404840513
2212	20	-0.6923263335076868
2212	21	-0.556707868265903
2212	22	-0.5550608445548448
2212	23	-0.5698000205049354
2212	24	-0.5136442751362367
2212	25	-0.40067067357901465
2213	1	-0.3151316829523836
2213	2	-0.7327133765686362
2213	3	-0.5570294082715783
2213	4	0.09276395682528715
2213	5	-0.1513076517309031
2213	6	-0.18531302511495795
2213	7	-0.3716188620408829
2213	8	-0.3260690269679145
2213	9	-0.47003103335939045
2213	10	-0.5303116526673222
2213	11	-0.5027456841323783
2213	12	-0.5601078156696033
2213	13	-0.49299665471438203
2213	14	-0.6230781831646865
2213	15	-0.5896248041520522
2213	16	-0.613254194573706
2213	17	-0.5807913060073631
2213	18	-0.6716236413573071
2213	19	-0.5869263911507758
2213	20	-5.869975967408781
2213	21	-3.0448467556998082
2213	22	-1.8860851278723842
2213	23	-1.3206507857968859
2213	24	-0.9455189666147774
2213	25	-0.7897652434425595
2214	1	-0.26381760563000256
2214	2	-0.7179110948635063
2214	3	-0.3540511204167025
2214	4	-0.02709862919759587
2214	5	-0.41841077556520645
2214	6	-0.40769780894378016
2214	7	-0.5067548177199583
2214	8	-0.5787877256276772
2214	9	-0.6322173550702601
2214	10	-0.8315127397217452
2214	11	-0.8310911524079714
2214	12	-0.7991480632786931
2214	13	-0.8358713659642873
2214	14	-0.9510201653692008
2214	15	-0.9008717532124138
2214	16	-0.9362913972645828
2214	17	-0.9490026553914888
2214	18	-0.8333778372784835
2214	19	-0.8222351769056431
2214	20	-0.6485339666845888
2214	21	-0.87400698854983
2214	22	-0.8424445723306562
2214	23	-0.9189320788386937
2214	24	-0.8825762527312335
2214	25	-0.9512122960916685
2215	1	-0.48753341156057317
2215	2	-0.4194304473895727
2215	3	-0.48722120325121665
2215	4	-0.06689633678053752
2215	5	-0.5618531153162678
2215	6	-0.4926744442559823
2215	7	-0.6647558196806926
2215	8	-0.919763168952116
2215	9	-1.1017827977900652
2215	10	-1.0731256373749076
2215	11	-1.0745697700476669
2215	12	-1.113871703505687
2215	13	-1.1249780661448128
2215	14	-1.3054054710967904
2215	15	-1.2805069013039443
2215	16	-1.3775795214386193
2215	17	-1.3471524830715182
2215	18	-1.1656595437212616
2215	19	-1.253859994436496
2215	20	-1.1799812816889528
2215	21	-1.3077069876860747
2215	22	-1.4835137335340463
2215	23	-1.3100120370327417
2215	24	-1.2196237508525556
2215	25	-1.4175710325846325
2216	1	-2.9048830072338667
2216	2	-2.3130854566515175
2216	3	-4.183359455458831
2216	4	-2.6429834065092273
2216	5	-2.7358478780393547
2216	6	-2.6851607373203095
2216	7	-2.5771619913354193
2216	8	-2.550480467588489
2216	9	-2.847624593342843
2216	10	-2.9391677736482613
2216	11	-2.9396633938118093
2216	12	-3.129242813674484
2216	13	-3.0232517733780604
2216	14	-3.5079954681954604
2216	15	-3.558497691433459
2216	16	-3.438283206130741
2216	17	-3.3205435454799215
2216	18	-3.5969184244021086
2216	19	-3.3691775415459517
2216	20	-3.3806236664353833
2216	21	-3.4898477990176353
2216	22	-3.3002120172843554
2216	23	-3.492938067935654
2216	24	-3.6090152893770444
2216	25	-3.6444325348796625
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68715.0
3	0.0
4	1104.0
5	8912.0
6	10049.0
7	7318.0
8	6002.0
9	5106.0
10	4663.0
11	4096.0
12	3862.0
13	3447.0
14	3391.0
15	3392.0
16	3441.0
17	3573.0
18	4099.0
19	4588.0
20	6116.0
21	7532.0
22	9604.0
23	11304.0
24	12762.0
25	14501.0
26	15539.0
27	17160.0
28	19429.0
29	23123.0
30	29084.0
31	36556.0
32	45777.0
33	60629.0
34	80303.0
35	109818.0
36	161198.0
37	272017.0
38	912739.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.31784737730374	18.578870088451403	13.33297438844345	27.770308145801405
2	14.096459111800197	20.17620259780727	37.8086246799229	27.91871361046963
3	17.260311539873697	24.322019298334613	29.079147682838684	29.338521478953
4	10.6975618159983	16.74065985617914	36.19992275040696	36.36185557741559
5	13.362249339769605	37.54127538879118	35.037197458244854	14.05927781319436
6	33.76051594612892	35.62136393629714	17.05038832568041	13.567731791893529
7	28.541873019763685	31.031018763147163	21.35747019976654	19.06963801732261
8	26.747337826601385	35.23087241915509	19.463319910469583	18.558469843773942
9	26.633560195061563	14.633485836131005	18.779749852538203	39.953204116269234
10	14.933133088384873	28.783034765771177	33.45977562854897	22.824056517294984
11	35.11076464371678	22.34967511984602	22.574449190511974	19.965111045925234
12	23.433040347283406	24.78960359982037	30.313708391644223	21.463647661252
13	29.18592823470021	20.650544129355357	25.08224101426817	25.081286621676263
14	22.048276343566997	20.71853466223788	26.614121259944557	30.619067734250567
15	25.164883681098814	27.93265924943331	22.61856029461327	24.283896774854604
16	23.522955375208717	27.065169997769665	25.92985896645053	23.482015660571086
17	23.232529771833015	27.33527525129718	26.40856038165822	23.023634595211586
18	23.469465194226505	26.521513404194476	27.204124413923015	22.804896987656008
19	23.98478470793814	26.671225292232688	26.963583582115046	22.38040641771413
20	24.355018636841024	26.00528692598354	26.725194869381387	22.914499567794053
21	23.8013630685668	25.963095990906847	26.794458321132286	23.44108261939407
22	23.850535598852606	26.0240719375534	25.87389229960185	24.251500163992144
23	23.18611353597737	26.08543878136323	26.344411149272755	24.38403653338664
24	23.439596211443522	26.95556829100582	26.231991573842144	23.372843923708515
25	23.59648589692654	25.645106931418134	26.153557926395905	24.604849245259423
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	302.0
1	302.0
2	433.0
3	564.0
4	564.0
5	564.0
6	1252.0
7	1940.0
8	1940.0
9	1940.0
10	2511.5
11	3083.0
12	3083.0
13	3083.0
14	4971.0
15	6859.0
16	6859.0
17	6859.0
18	12474.5
19	18090.0
20	18090.0
21	18090.0
22	27949.5
23	37809.0
24	37809.0
25	37809.0
26	53703.5
27	69598.0
28	69598.0
29	69598.0
30	88972.0
31	108346.0
32	108346.0
33	108346.0
34	128273.5
35	148201.0
36	148201.0
37	148201.0
38	167166.0
39	186131.0
40	186131.0
41	186131.0
42	204380.0
43	222629.0
44	222629.0
45	222629.0
46	254984.5
47	287340.0
48	287340.0
49	287340.0
50	283422.0
51	279504.0
52	279504.0
53	279504.0
54	252868.0
55	226232.0
56	226232.0
57	226232.0
58	196489.0
59	166746.0
60	166746.0
61	166746.0
62	139288.0
63	111830.0
64	111830.0
65	111830.0
66	88174.0
67	64518.0
68	64518.0
69	64518.0
70	47571.5
71	30625.0
72	30625.0
73	30625.0
74	21655.0
75	12685.0
76	12685.0
77	12685.0
78	8902.0
79	5119.0
80	5119.0
81	5119.0
82	3530.0
83	1941.0
84	1941.0
85	1941.0
86	1288.0
87	635.0
88	635.0
89	635.0
90	417.0
91	199.0
92	199.0
93	199.0
94	111.0
95	23.0
96	23.0
97	23.0
98	11.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1829780672433096
2	0.05997140057329444
3	0.0
4	0.0
5	0.017880920103930335
6	0.3604311310837194
7	0.0012556825915681416
8	0.028328199265777274
9	0.030287064108623574
10	0.0027122743977871858
11	0.0030638655234262653
12	0.009191596570278796
13	0.007735004764059751
14	0.001808182931858124
15	0.0
16	0.01089932489481147
17	0.023958423847120144
18	0.06208094732712892
19	0.029282518035369062
20	0.0
21	0.0
22	0.0
23	1.50681910988177E-4
24	3.01363821976354E-4
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1990949.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.43451065947853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51945511971452	48.39889246988784
2	13.743828209921219	17.161783777487365
3	4.277327529437799	8.011585536922958
4	1.8079480140581952	4.515133982219977
5	0.8533166067390352	2.6638202389679173
6	0.5031057090569413	1.884669525297606
7	0.2878399314363318	1.257980168723966
8	0.19644127578963383	0.9811771941799564
9	0.142390060184584	0.8001048357358381
>10	0.6125931640489056	6.6511240004873935
>50	0.03409556164397875	1.474016793712375
>100	0.01826612621000393	2.0700583764867715
>500	0.001696345879446394	0.8223195754150154
>1k	0.0015347891290229245	2.184732394198283
>5k	0.0	0.0
>10k+	1.615567504234657E-4	1.122601130276774
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11190	0.5620435279859002	No Hit
CCCATGTACTCTGCGTTGATACCAC	11069	0.5559660242427104	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4497	0.22587218457127733	No Hit
GAGTACATGGGAAGCAGTGGTATCA	4340	0.21798649789622937	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3309	0.1662021478199592	No Hit
CATGTACTCTGCGTTGATACCACTG	3023	0.15183713897241968	No Hit
GTATCAACGCAGAGTACATGGGAAG	2998	0.15058145638085155	No Hit
GCGTTGATACCACTGCTTCCCATGT	2933	0.14731668164277437	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2844	0.14284645161679177	No Hit
TATCAACGCAGAGTACATGGGAAGC	2755	0.13837622159080923	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2520	0.12657280523006867	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2103	0.10562801960271206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.022730366272567E-5	0.0
4	0.0	0.0	0.0	1.0045460732545133E-4	0.0
5	0.0	0.0	0.0	1.0045460732545133E-4	0.0
6	0.0	0.0	0.0	1.50681910988177E-4	0.0
7	0.0	0.0	0.0	1.50681910988177E-4	0.0
8	0.0	0.0	0.0	1.50681910988177E-4	0.0
9	0.0	0.0	0.0	1.50681910988177E-4	0.0
10	0.0	0.0	0.0	1.50681910988177E-4	0.0
11	0.0	0.0	0.0	2.0090921465090267E-4	0.0
12	0.0	0.0	0.0	2.0090921465090267E-4	0.0
13	0.0	0.0	0.0	2.0090921465090267E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCCGT	45	6.663665E-4	14.80679	6
CGCCCTA	40	0.005304602	14.239933	16
TGCGCAT	65	5.5318385E-5	13.133989	10
TTGCGCG	60	4.1243824E-4	12.655173	18
ATCGTCC	60	0.0059364275	11.068269	8
TAGGACC	310	0.0	11.053917	4
GTCCTAC	345	0.0	11.046697	1
GCGTGCG	155	3.2014214E-10	11.017263	9
ACCGTGG	125	2.2831045E-7	10.625539	8
ACTGCGC	90	9.649352E-5	10.541209	8
AGGCGTG	155	3.745299E-9	10.405193	7
GTAGGAC	490	0.0	10.295683	3
CGTGCGC	130	3.9227234E-7	10.215324	10
TAGACCG	75	0.002605386	10.153228	5
AAGGCGT	150	2.3719622E-8	10.153228	6
ATAAGAC	200	2.3646862E-11	9.994583	3
TGTAGGA	520	0.0	9.891733	2
TAGGACG	270	0.0	9.871194	4
GTGCGCC	135	6.5669155E-7	9.838215	11
CTAAGGT	175	2.4883775E-9	9.790612	4
>>END_MODULE