FastQCFastQC Report
Thu 26 May 2016
SRR1513065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701198
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC82750.4864219214929714No Hit
CCCATGTACTCTGCGTTGATACCAC63360.37244341928452773No Hit
GAGTACATGGGAAGCAGTGGTATCA36510.21461346651007115No Hit
GTATCAACGCAGAGTACATGGGAAG22330.13126044117145683No Hit
GCGTTGATACCACTGCTTCCCATGT22100.12990845274918028No Hit
CATGTACTCTGCGTTGATACCACTG21460.12614639800893251No Hit
GTATCAACGCAGAGTACTTTTTTTT21170.12444171695475777No Hit
GCTTCCCATGTACTCTGCGTTGATA18100.10639561062263181No Hit
TATCAACGCAGAGTACATGGGAAGC17380.10216329903985309No Hit
ACGCAGAGTACATGGGAAGCAGTGG17140.10075252851226017No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCG400.005282898414.24856717
TAGGACC3850.013.0816084
GTAGGAC7800.012.3043863
CTACACT2250.011.8255534
AAGACGT658.0110884E-411.6945715
GTATAAG2300.011.5800581
AAGGCGT1451.2205419E-911.1361566
TGTAGGA9650.011.1274862
GTGTAGG3000.010.7804811
TAGGACA3900.010.7209684
CTGTAGG9000.010.5690991
CTAGACT1803.45608E-1110.558534
TATGGCG5400.010.55449516
GGACTAT1355.6998033E-810.5538746
GTCCTAA3200.010.4039571
CTAGACA1752.3464963E-1010.3171914
TACTAGC750.00264160910.13618852
CCGTGCC750.002651940310.1314219
TAGAACA3300.010.0785974
GTCCTAC6150.010.0535341
Produced by FastQC (version 0.11.5)