FastQCFastQC Report
Thu 26 May 2016
SRR1513065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701198
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC92640.5445574236508625No Hit
CCCATGTACTCTGCGTTGATACCAC84330.49570949413295806No Hit
GAGTACATGGGAAGCAGTGGTATCA38590.22684014441587633No Hit
GTATCAACGCAGAGTACTTTTTTTT35210.2069717928189429No Hit
CATGTACTCTGCGTTGATACCACTG27030.15888803067015128No Hit
TATCAACGCAGAGTACTTTTTTTTT24490.143957375919793No Hit
GCGTTGATACCACTGCTTCCCATGT23740.13954871802106514No Hit
GTATCAACGCAGAGTACATGGGAAG23690.1392548074944833No Hit
ACGCAGAGTACATGGGAAGCAGTGG23010.13525762433297006No Hit
GGTATCAACGCAGAGTACTTTTTTT22380.1315543516980387No Hit
GCTTCCCATGTACTCTGCGTTGATA22030.1294969780119657No Hit
TATCAACGCAGAGTACATGGGAAGC20640.12132626537299009No Hit
ACTCTGCGTTGATACCACTGCTTCC17460.10263355588238407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT801.9552099E-613.0887086
TAGGACC4000.012.8507314
TGGCGAG5450.012.016218
TATGGCG5250.011.93477416
AGGACCG802.8085198E-511.8988255
TACACCG1052.650395E-711.7855025
ACCGTGC1302.6757334E-911.6751198
GTCCTAC6700.011.3787991
GGCGAGA4600.011.34433219
TTTAGAC1604.3655746E-1111.3038843
GTCCTAG2200.011.2624251
CGTACCC600.005789018711.105573
TGCACCG700.001466791510.8789255
ATGGCGA6150.010.80505517
GTTTTAG1502.0245352E-910.8003781
TTACCCT1252.1788946E-710.6613464
GACCGTG1355.8056685E-810.5406587
TGTAGGA7450.010.4866372
GTTACCC1002.346251E-510.4709663
CTACACT3000.010.4709654
Produced by FastQC (version 0.11.5)