FastQCFastQC Report
Thu 26 May 2016
SRR1513064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217350
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC176790.7973030870182876No Hit
CCCATGTACTCTGCGTTGATACCAC145710.6571357701761111No Hit
GAGTACATGGGAAGCAGTGGTATCA79530.3586713870160327No Hit
GCGTTGATACCACTGCTTCCCATGT50870.22941799896272577No Hit
GTATCAACGCAGAGTACATGGGAAG50000.22549439646424785No Hit
CATGTACTCTGCGTTGATACCACTG46860.2113333483662931No Hit
TATCAACGCAGAGTACATGGGAAGC37390.16862470967596455No Hit
ACGCAGAGTACATGGGAAGCAGTGG36090.16276185536789411No Hit
GCTTCCCATGTACTCTGCGTTGATA34860.1572146932148736No Hit
ACTCTGCGTTGATACCACTGCTTCC32570.14688704985681106No Hit
GTATCAACGCAGAGTACTTTTTTTT26590.11991792003968701No Hit
GGTATCAACGCAGAGTACATGGGAA22910.10332153245991837No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGC456.765581E-414.77730519
CGTTTAT3000.013.29837414
GCGTTTA3150.013.26821813
TGCGTTT3200.013.06090412
TATCGAC604.094357E-412.66626119
GTAGGAC7050.012.532083
GTCTACG550.00304405112.1041751
TAGGACC6200.011.9523094
TGTAGGA7750.011.7689582
CACGACT600.00587445411.0839784
CGATACT600.00587445411.0839784
GGATCGT600.00588235111.0819776
TTCGTTG803.7731574E-410.68715816
GTATTAG1909.094947E-1210.511521
GTCCTAC7600.010.511521
TGGCGAG4450.010.24709318
TAGACTG2600.010.2311335
TATGGCG4550.010.23044216
GTCTTAC2350.010.1174721
TCGTTGA856.597248E-410.05850117
Produced by FastQC (version 0.11.5)