FastQCFastQC Report
Thu 26 May 2016
SRR1513064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2217350
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC206200.9299388910185582No Hit
CCCATGTACTCTGCGTTGATACCAC192250.867025954405033No Hit
GAGTACATGGGAAGCAGTGGTATCA81560.3678264595124811No Hit
CATGTACTCTGCGTTGATACCACTG57390.25882246826166366No Hit
GTATCAACGCAGAGTACATGGGAAG52920.23866326921775996No Hit
GCGTTGATACCACTGCTTCCCATGT52890.2385279725798814No Hit
ACGCAGAGTACATGGGAAGCAGTGG46060.20772543802286514No Hit
TATCAACGCAGAGTACATGGGAAGC44350.20001352966378785No Hit
GCTTCCCATGTACTCTGCGTTGATA42960.19374478544208176No Hit
ACTCTGCGTTGATACCACTGCTTCC40420.18228967010169797No Hit
GTATCAACGCAGAGTACTTTTTTTT38690.17448756398403498No Hit
GGTATCAACGCAGAGTACATGGGAA30440.1372809885674341No Hit
TATCAACGCAGAGTACTTTTTTTTT28170.12704354296795725No Hit
GGTATCAACGCAGAGTACTTTTTTT23550.10620786073466074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCA400.00530081114.241569512
AAGACCG500.001480154613.3234065
TAGGACC4500.012.0545114
GTAGGAC6000.011.5786753
GTCCTAC7000.011.5675311
GGCGAGA2850.011.31921919
GCGAATC600.00590293911.076776515
GCGCCGG600.00593673811.06828311
TCGAACT1752.1827873E-1110.84362219
TATGGCG4350.010.694818516
TGGCGAG4200.010.62274518
TGTAGGA7100.010.5966972
TCCTACA8450.010.5943162
TTATACT2950.010.3232214
TTAGGAC3900.010.24877553
TAGGACT3300.010.093494
TAAGACT3250.09.9559534
AAGGCGT2300.09.930496
TAAACTG2400.09.9132495
CCTACAC4000.09.7546373
Produced by FastQC (version 0.11.5)