FastQCFastQC Report
Thu 26 May 2016
SRR1513063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905896
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC75800.3977132015597913No Hit
CCCATGTACTCTGCGTTGATACCAC60090.31528477944232003No Hit
GAGTACATGGGAAGCAGTGGTATCA32690.17152037676767254No Hit
GTATCAACGCAGAGTACTTTTTTTT22650.11884174162703526No Hit
GTATCAACGCAGAGTACATGGGAAG20450.10729861440498328No Hit
GCGTTGATACCACTGCTTCCCATGT20420.10714120812468256No Hit
CATGTACTCTGCGTTGATACCACTG20330.10666898928378044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4650.014.91511254
GTCCTAC9300.012.9884231
GTAGGAC9400.012.9371543
GGCGTGC1207.421477E-1012.664978
TCCAACG1405.2750693E-1112.21361118
TGGCGAG6800.011.87434418
TGTAGGA11100.011.640832
GACTGCG752.0768662E-411.3984737
TAGGACA5300.011.1140524
GCGAATT600.005882348411.08184913
CTAGGAC3200.010.9852093
CTGTAGG11200.010.9548731
TATGGCG7550.010.94612816
AAGGCGT1658.0035534E-1110.9385026
CTAGCAC2000.010.925833
TCCTACA11400.010.9177832
AGGACCT8450.010.7937195
CCTACAG11000.010.7099053
ACACCGT803.7743596E-410.6866286
CGGTTTC803.776191E-410.68606813
Produced by FastQC (version 0.11.5)