##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513063_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1905896
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.20454421437476	31.0	30.0	34.0	23.0	34.0
2	28.906274529145346	31.0	30.0	34.0	16.0	34.0
3	29.24318378337538	31.0	30.0	34.0	22.0	34.0
4	32.08369449329869	35.0	32.0	37.0	19.0	37.0
5	32.28900055407011	35.0	33.0	37.0	19.0	37.0
6	32.7530883112195	37.0	33.0	37.0	26.0	37.0
7	32.73541263531693	37.0	33.0	37.0	25.0	37.0
8	32.727358155953944	37.0	35.0	37.0	25.0	37.0
9	34.094097999051364	39.0	35.0	39.0	25.0	39.0
10	33.578697893274345	38.0	34.0	39.0	18.0	39.0
11	33.976249491053025	39.0	34.0	39.0	25.0	39.0
12	33.806060771416696	39.0	34.0	39.0	22.0	39.0
13	33.90039173176291	39.0	35.0	39.0	23.0	39.0
14	34.216384839466585	39.0	33.0	41.0	16.0	41.0
15	34.763251510050914	40.0	34.0	41.0	20.0	41.0
16	34.348668028056096	39.0	34.0	41.0	17.0	41.0
17	33.892036606404545	39.0	33.0	41.0	11.0	41.0
18	33.30567092852915	39.0	32.0	41.0	10.0	41.0
19	34.19924906710545	39.0	34.0	41.0	18.0	41.0
20	34.46090657622452	39.0	34.0	41.0	18.0	41.0
21	34.477080071525414	40.0	34.0	41.0	17.0	41.0
22	34.08060618207919	40.0	34.0	41.0	10.0	41.0
23	32.40424031531626	39.0	31.0	41.0	2.0	41.0
24	33.49217743255666	39.0	33.0	41.0	2.0	41.0
25	33.23412242850607	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.808758177530283
1101	2	1.1616381219530716
1101	3	0.9158889482946897
1101	4	0.9691531585238309
1101	5	1.1576824348880521
1101	6	0.9478634568954192
1101	7	0.8919311787629951
1101	8	0.9077187645324116
1101	9	1.050857744401
1101	10	1.3272420528561142
1101	11	1.0204615613700199
1101	12	1.1037870139031298
1101	13	0.9905842745718445
1101	14	0.16427569946767306
1101	15	0.7942874037828673
1101	16	1.4563251714139511
1101	17	2.0329260744091826
1101	18	-4.416076028550613
1101	19	-0.4511066276830604
1101	20	0.5710791490223741
1101	21	0.902574230968888
1101	22	1.5900270585416436
1101	23	3.2579911014423075
1101	24	2.2286920317729724
1101	25	2.4559398755376307
1102	1	1.2128022854085891
1102	2	1.4990297343705912
1102	3	1.272269976592817
1102	4	1.771746489200062
1102	5	1.5054013135913848
1102	6	1.2343556405896763
1102	7	1.1344129111929178
1102	8	1.1535732439620219
1102	9	1.2547813970293191
1102	10	1.6502658339109857
1102	11	1.1994332632432787
1102	12	1.3364091298413072
1102	13	1.1972323415307926
1102	14	2.181546856635208
1102	15	1.601498776175589
1102	16	1.892980766826021
1102	17	1.5333839814992132
1102	18	1.9849141844603295
1102	19	1.5837651671887372
1102	20	1.5943163838658307
1102	21	1.5464996549979375
1102	22	1.9382065014361132
1102	23	3.57090549850178
1102	24	2.3724465156310828
1102	25	2.717531479098149
1103	1	0.8129648956192419
1103	2	1.221012851535587
1103	3	0.9424718314755225
1103	4	1.383522002636525
1103	5	1.2838146321419686
1103	6	0.9417070065271744
1103	7	0.8286152588338354
1103	8	0.7067620348858838
1103	9	0.8571384238201105
1103	10	1.1879723963944855
1103	11	0.9393628692126939
1103	12	1.0330591952490948
1103	13	0.8833601418933199
1103	14	1.6325191741826757
1103	15	1.1541566787854478
1103	16	1.4665074490966958
1103	17	1.992614340098136
1103	18	1.9889346221938737
1103	19	1.3178707230376006
1103	20	1.3276727125824905
1103	21	1.3267581184135722
1103	22	1.70476135891753
1103	23	3.224660257431559
1103	24	2.1160422427134264
1103	25	2.3005401199654045
1104	1	0.6788474473796775
1104	2	1.148198247613177
1104	3	0.8661942142207586
1104	4	1.4260495386882965
1104	5	1.2837298985866354
1104	6	0.8695344946574153
1104	7	0.7105804747678874
1104	8	0.8309310576161266
1104	9	0.9548816397221529
1104	10	1.2556683874616823
1104	11	0.9150584610907018
1104	12	0.8884213700016943
1104	13	0.7737502269375653
1104	14	1.7001339622593505
1104	15	1.118521040605259
1104	16	1.3764048461760225
1104	17	1.834567900460911
1104	18	2.437945734528917
1104	19	1.5883995451564488
1104	20	1.0417849920751792
1104	21	1.108416928396693
1104	22	1.3636544279951508
1104	23	2.767762098691719
1104	24	1.8038762929867858
1104	25	1.8803941993682756
1105	1	0.4449041161621068
1105	2	0.9115934004174022
1105	3	0.5638904160784257
1105	4	0.8072940128374739
1105	5	0.7750160434308313
1105	6	0.49916937866183986
1105	7	0.3149351604792088
1105	8	0.31840112519439145
1105	9	0.3859845259852577
1105	10	0.8732353737344525
1105	11	0.5074513874411792
1105	12	0.3795744040178164
1105	13	0.3206570815847485
1105	14	1.1612540408367735
1105	15	0.5307295454063592
1105	16	0.9421626461202592
1105	17	1.4017293190580347
1105	18	1.3870070229576612
1105	19	0.9531834642474806
1105	20	0.6072236763236845
1105	21	0.4148229920853197
1105	22	0.9587008136158204
1105	23	2.564420946848692
1105	24	1.515200241306161
1105	25	1.518050061639201
1106	1	-0.38727359563985075
1106	2	0.009039679656673627
1106	3	-0.211940530973024
1106	4	-2.9227232786146153
1106	5	-0.4835268220682529
1106	6	-0.35128942254062423
1106	7	-0.3742972000458522
1106	8	-0.27581706546997253
1106	9	-0.23641361073394052
1106	10	-0.11422504166012715
1106	11	-0.23126792395669327
1106	12	-0.25423909499934894
1106	13	-0.1946551550180473
1106	14	0.4505522983395025
1106	15	-0.07214182196657504
1106	16	-0.9188917078877168
1106	17	-5.9432627649078675
1106	18	-6.8662362921712194
1106	19	-2.8065078086486714
1106	20	-1.469878389366805
1106	21	-1.1263676451983002
1106	22	-1.3906930749243855
1106	23	-5.164011836874046
1106	24	-2.1874213159699885
1106	25	-0.8328234340216127
1107	1	0.6998115120997248
1107	2	1.0072871981350104
1107	3	0.7336820171698655
1107	4	1.0013269551224795
1107	5	1.0370126710874317
1107	6	0.6349931828574356
1107	7	0.5572930661605469
1107	8	0.5682588058046392
1107	9	0.5728439618115644
1107	10	1.0225908581246728
1107	11	0.8216272786380898
1107	12	0.8863651203937195
1107	13	0.8554043352097409
1107	14	1.558485226416515
1107	15	0.9581649330803117
1107	16	-5.8285349253457355
1107	17	-0.7903206284479438
1107	18	0.8283822648667751
1107	19	0.9347780641658474
1107	20	0.7823689233298978
1107	21	0.9296370698252119
1107	22	1.046469062819945
1107	23	2.8776153250128047
1107	24	1.9721233265603786
1107	25	1.624408167684578
1108	1	0.5103281391687808
1108	2	0.8659894826781205
1108	3	0.5915412956338599
1108	4	1.1988625901664847
1108	5	0.9741911579044285
1108	6	0.5274254725149063
1108	7	0.48144496030420925
1108	8	0.5394741820200863
1108	9	0.672029972286694
1108	10	1.0700683815157674
1108	11	0.6655744321545143
1108	12	0.6871750031672832
1108	13	0.5763371198092884
1108	14	1.1439353880721654
1108	15	0.9603491412975416
1108	16	1.2491304740627598
1108	17	1.311159328024985
1108	18	1.050793704345942
1108	19	1.0976912050294416
1108	20	0.897551627758034
1108	21	0.9627836020776854
1108	22	1.235231285734855
1108	23	3.1025325560211527
1108	24	1.7584803621322536
1108	25	2.0268058372042006
1109	1	0.17515829951333117
1109	2	0.5194595516764693
1109	3	0.34566512699985097
1109	4	0.2452060686698303
1109	5	0.16383847348864577
1109	6	0.07258877512162343
1109	7	0.1690444030739826
1109	8	0.07338053721583293
1109	9	0.1373753923567591
1109	10	0.7180991890108928
1109	11	0.2748431278551777
1109	12	0.2916872341177097
1109	13	0.08297001076591215
1109	14	-0.9818940035798462
1109	15	-0.2012455536315585
1109	16	-0.38305365338496955
1109	17	0.6856931249119214
1109	18	1.3758291061732137
1109	19	0.6636641702795885
1109	20	0.47213682413109126
1109	21	0.3488005271120471
1109	22	0.18223128630979346
1109	23	-4.222668428043281
1109	24	-1.5942384349587897
1109	25	-0.15336660979654226
1110	1	0.5646989073052104
1110	2	0.9138018141426691
1110	3	0.742933387256226
1110	4	1.3003759153294894
1110	5	0.8011461243361993
1110	6	0.5190788803129109
1110	7	0.3400508843308856
1110	8	0.39136119674996905
1110	9	0.5056360978840146
1110	10	0.8129998591450089
1110	11	0.4757580495680642
1110	12	0.5757131591453373
1110	13	0.42230254910099774
1110	14	1.2548934174817674
1110	15	0.6087718734486813
1110	16	1.0688039251491759
1110	17	1.2579952961105292
1110	18	2.0988559834021245
1110	19	1.2816314675550373
1110	20	0.9011936732431209
1110	21	0.7505147140129935
1110	22	1.092747608477218
1110	23	2.371377618973902
1110	24	1.348457911642484
1110	25	1.6844677835344513
1111	1	0.39629509134686813
1111	2	0.732469043831049
1111	3	0.4223694563155611
1111	4	0.9964025469559274
1111	5	0.7181052300758708
1111	6	0.34934663035924984
1111	7	0.16714529887573093
1111	8	-0.07271425865066306
1111	9	0.12385229822183419
1111	10	0.354358568671266
1111	11	0.21140099588517103
1111	12	0.3242758801138734
1111	13	0.13985033600602037
1111	14	-0.5822116804875108
1111	15	-0.026331481251496314
1111	16	0.37681985936306717
1111	17	0.919134033752897
1111	18	1.5764159836818905
1111	19	0.6639627708628808
1111	20	0.5099855982528254
1111	21	0.4116084274034719
1111	22	0.6643127826117947
1111	23	0.5978784565124116
1111	24	0.5362501321511601
1111	25	0.12137678558720921
1112	1	0.48063206403994485
1112	2	0.7728262459331994
1112	3	0.6035560711377812
1112	4	1.0999047829431134
1112	5	0.842270480342286
1112	6	0.30879705258693235
1112	7	0.3850170420598573
1112	8	0.27903423209055234
1112	9	0.43573538245110655
1112	10	0.6771636404408525
1112	11	0.3264661821421626
1112	12	0.4204923761321737
1112	13	0.38944973430008645
1112	14	0.9067330151984976
1112	15	0.5196695366459565
1112	16	0.8741791450283429
1112	17	1.0559776223451536
1112	18	1.1392520440018643
1112	19	0.66479350372299
1112	20	0.597988565093857
1112	21	0.6426618123033236
1112	22	0.725652394716434
1112	23	2.45413561631797
1112	24	1.2566683559895893
1112	25	1.3463818446765004
1113	1	0.4846189793529412
1113	2	0.8713956190768997
1113	3	0.5287238773020349
1113	4	0.7547906790527676
1113	5	0.8982436485613761
1113	6	0.4138573681765081
1113	7	0.3635236586015367
1113	8	0.3453608417070626
1113	9	0.37247552323407973
1113	10	0.7628681252366789
1113	11	0.47849491081556295
1113	12	0.33081538552415424
1113	13	0.3195673115253328
1113	14	1.0680716793302452
1113	15	0.5116351646098494
1113	16	0.9360285937036323
1113	17	1.3168791624412037
1113	18	1.839607765111353
1113	19	0.8994662037408361
1113	20	0.6474023995050402
1113	21	0.6709004551434177
1113	22	0.8711834063279156
1113	23	0.7625621633499335
1113	24	0.6718682286698154
1113	25	1.2132216045755086
1114	1	-0.4740185440706739
1114	2	-3.2250976068903228
1114	3	-1.3005183703651753
1114	4	-0.09898074261914047
1114	5	0.12891139250607608
1114	6	-0.19725501358841768
1114	7	-0.2509877871229591
1114	8	-0.17566730280208986
1114	9	-0.18882640166460618
1114	10	0.1501833359734306
1114	11	-0.2116087940386251
1114	12	-0.10465862124269876
1114	13	-0.29091178819929553
1114	14	0.5163101542194326
1114	15	-0.15911987502479974
1114	16	0.286545149938668
1114	17	0.584142268097601
1114	18	0.775183205512576
1114	19	0.23475622574560617
1114	20	0.08218674266241521
1114	21	-0.14063127783985152
1114	22	0.083075768593325
1114	23	1.6136186528951413
1114	24	0.6347336251200133
1114	25	0.6273653999167976
1115	1	0.12416379694827384
1115	2	0.4480298458651326
1115	3	0.31417930705448427
1115	4	0.50877774302419
1115	5	0.35133260575875624
1115	6	-0.0883924551651063
1115	7	-0.11290232739180794
1115	8	-0.07265201308729274
1115	9	-0.030484735007419772
1115	10	0.09251356390927157
1115	11	0.11075822632770382
1115	12	0.021934037994277844
1115	13	-0.07329834530278845
1115	14	0.8125575682695256
1115	15	0.08285596390248884
1115	16	0.5536416303376654
1115	17	1.0459514832872046
1115	18	1.3416957448944515
1115	19	0.6760330477862411
1115	20	0.1896631998807976
1115	21	0.18718026120192377
1115	22	0.4919187530548257
1115	23	2.2518409649160915
1115	24	1.020244715945985
1115	25	0.7503777716326354
1116	1	0.11258250518880786
1116	2	0.2178339763209003
1116	3	0.25073316134813695
1116	4	0.866839710585424
1116	5	0.18354758679383565
1116	6	0.01996319159048454
1116	7	0.024911326635148612
1116	8	0.015662665152561317
1116	9	0.05358509021503011
1116	10	0.549562043319554
1116	11	0.05329505688035141
1116	12	0.11932828309753774
1116	13	0.005009433352824999
1116	14	0.6965816345913325
1116	15	0.16657759249035564
1116	16	0.43928799268380914
1116	17	0.8156405015085397
1116	18	1.4143916233759697
1116	19	0.4907874333121285
1116	20	0.20642597215519487
1116	21	0.2121088522925234
1116	22	0.5164419488850456
1116	23	1.8955557296688639
1116	24	0.9548130629414544
1116	25	1.1094893339773506
1201	1	0.8733081931227282
1201	2	1.1924746427002084
1201	3	0.9744735221308787
1201	4	1.5183254724729096
1201	5	1.2008903486192608
1201	6	0.7033652609011725
1201	7	0.7344321942629222
1201	8	0.7247998715774386
1201	9	0.9901952402118752
1201	10	1.3089118349478994
1201	11	0.8893109248981688
1201	12	1.0148914673374776
1201	13	0.8986383683997516
1201	14	1.8152703992626158
1201	15	1.3442568009343248
1201	16	1.6806300211230223
1201	17	2.10240942848138
1201	18	2.5598714190983003
1201	19	1.6981661640870769
1201	20	1.4402332544297352
1201	21	1.2859415981007487
1201	22	1.6775251056571676
1201	23	3.214892242907112
1201	24	2.010191124102903
1201	25	2.0807499326323153
1202	1	0.8839305086129485
1202	2	1.1077256904334831
1202	3	0.90277601854757
1202	4	1.573195473772067
1202	5	1.2394059953154866
1202	6	0.9124575238237398
1202	7	0.7932938164467487
1202	8	0.80177102119751
1202	9	0.804004875544301
1202	10	1.308713272971744
1202	11	1.030677173838619
1202	12	1.016813192155837
1202	13	0.8472186940338844
1202	14	1.6278163901800298
1202	15	1.16250019515374
1202	16	1.4823547594569746
1202	17	1.8723258636487046
1202	18	2.461376801465768
1202	19	1.6360981661132072
1202	20	1.2654488248396518
1202	21	1.3051832288947622
1202	22	1.608254734642614
1202	23	3.2506132673318646
1202	24	2.014922225798017
1202	25	1.9893317737394725
1203	1	0.8286925640064737
1203	2	1.1211723912794724
1203	3	0.8260073394857947
1203	4	1.4650826854532184
1203	5	1.3144950205357517
1203	6	0.8164177778786055
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2211	16	-0.2708072538622943
2211	17	0.14415641845131688
2211	18	-5.393008377786028
2211	19	-1.642682804056868
2211	20	-0.7977096705886524
2211	21	-0.47257780806768324
2211	22	-0.25451411347601294
2211	23	-5.027458673248372
2211	24	-1.9812740908437156
2211	25	-1.2139692676049663
2212	1	0.1088931298349678
2212	2	0.31029259157796574
2212	3	0.11114576834839696
2212	4	0.63437630324335
2212	5	0.4934963581329299
2212	6	0.07184652340420428
2212	7	-0.0768660916184487
2212	8	-0.18578036734668046
2212	9	-0.3670704924579766
2212	10	-4.112263380599739
2212	11	-1.3409159560095105
2212	12	-0.5354818252332691
2212	13	-0.3205642916027003
2212	14	0.39156447002137895
2212	15	0.013446410223615146
2212	16	0.08073009221075012
2212	17	0.679465454700896
2212	18	1.234652919232083
2212	19	0.40774409886912366
2212	20	0.06678440315022982
2212	21	-0.16661238194501493
2212	22	0.09972001067749403
2212	23	1.4746745373302659
2212	24	0.627030738101702
2212	25	0.43980902541796496
2213	1	-1.586899555268861
2213	2	-3.9486124293691347
2213	3	-1.924216340869112
2213	4	-3.520361416078572
2213	5	-1.4135568190252847
2213	6	-1.1529873496801457
2213	7	-1.0129687293594571
2213	8	-0.9199313675913743
2213	9	-0.9157181832263603
2213	10	-0.9972317574890468
2213	11	-0.9127463084484901
2213	12	-0.8136230411664727
2213	13	-1.1500448418439504
2213	14	-6.395864701264156
2213	15	-3.0095912632071347
2213	16	-2.117655836005703
2213	17	-1.000729456516666
2213	18	-0.20487414044995234
2213	19	-0.8836049796936081
2213	20	-0.8545670962371616
2213	21	-1.1498320613337825
2213	22	-1.0345493313197167
2213	23	-6.0505216321936395
2213	24	-3.367331048048918
2213	25	-2.3440644507904658
2214	1	-0.27956127839466305
2214	2	0.034622537589850566
2214	3	-0.19603991066648874
2214	4	0.15715205639551755
2214	5	0.0023613056097460117
2214	6	-0.2387984383620676
2214	7	-0.3552698185287255
2214	8	-0.4996147569691729
2214	9	-0.5139272397727055
2214	10	0.12899974591337582
2214	11	-0.4653357679848966
2214	12	-0.23604954853176707
2214	13	-0.35234463143139294
2214	14	0.1996402418492167
2214	15	-0.21959470880894827
2214	16	0.19094611319603416
2214	17	0.4646018470750519
2214	18	1.0595877685502515
2214	19	0.04137067452187182
2214	20	-0.020942853222202018
2214	21	-0.11878252931148836
2214	22	0.40036320767842426
2214	23	1.7717492245984232
2214	24	0.7235479822578412
2214	25	0.4197463848149994
2215	1	-2.7377373242354963
2215	2	-4.766433239901531
2215	3	-2.7488587496613484
2215	4	-4.358075432289052
2215	5	-5.206986338980453
2215	6	-2.7318299073932693
2215	7	-2.072863961399932
2215	8	-2.3724592232708304
2215	9	-2.0430654299378688
2215	10	-3.4019998426163625
2215	11	-2.359118983705283
2215	12	-2.105479408916082
2215	13	-1.9736246663919523
2215	14	-1.2339924680181227
2215	15	-1.8870750486446681
2215	16	-2.709851661322382
2215	17	-7.367802177178213
2215	18	-3.35318115444797
2215	19	-2.5654921468955045
2215	20	-2.325458337725344
2215	21	-2.280971934137675
2215	22	-2.0611858439835444
2215	23	-6.6475265094888165
2215	24	-4.068752725479239
2215	25	-3.7714182078191456
2216	1	-0.5928477370835381
2216	2	-0.08043145421340725
2216	3	-0.3619120596944825
2216	4	-2.8309535657806926
2216	5	-0.8770888148636544
2216	6	-0.5389017193343548
2216	7	-0.48767235896252714
2216	8	-0.487421407909828
2216	9	-0.5487823556572451
2216	10	-0.2093882871466448
2216	11	-0.6855479933169235
2216	12	-1.3560375138334066
2216	13	-0.8294553177884438
2216	14	-6.357894338946341
2216	15	-2.7935069718126186
2216	16	-1.2581883239921936
2216	17	-0.4144032929547308
2216	18	0.41224361915818264
2216	19	-0.1620877842782491
2216	20	-0.7250188488027334
2216	21	-0.7837027741518625
2216	22	-0.2077664080455648
2216	23	1.0119141454753446
2216	24	0.23669702795177017
2216	25	0.23493538773012546
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97059.0
3	4.0
4	814.0
5	4836.0
6	5732.0
7	5119.0
8	4750.0
9	4429.0
10	4337.0
11	4168.0
12	4093.0
13	4032.0
14	4082.0
15	4026.0
16	4283.0
17	4587.0
18	5129.0
19	6004.0
20	7409.0
21	9253.0
22	11515.0
23	13908.0
24	16382.0
25	19115.0
26	21689.0
27	25110.0
28	27795.0
29	33685.0
30	39491.0
31	45513.0
32	58350.0
33	77088.0
34	100061.0
35	132082.0
36	186564.0
37	261189.0
38	652213.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.70363298762216	17.69592999283015	12.511417923579357	25.089019095968336
2	14.051380629352606	19.043987072167102	42.20306482236303	24.701567476117262
3	19.9197802722663	23.343814803909375	28.313879367038847	28.422525556785477
4	10.907770218318106	15.910134626961398	38.59313224575784	34.58896290896266
5	12.853739481044753	37.540880440424914	35.86283382084565	13.742546257684682
6	33.58301224761403	35.60367438916744	17.571584359915487	13.241729003303046
7	29.515768283326356	30.663530537896467	20.679242668984127	19.141458509793054
8	25.906094017242037	36.77313445211889	19.471214022448137	17.849557508190937
9	26.531333087711236	15.578949136281429	19.212844146478943	38.676873629528394
10	14.361895324253792	29.39976989727257	34.07929516343382	22.159039615039816
11	35.31755423856098	22.726693070699447	22.547319178994467	19.40843351174511
12	23.424231880772343	24.773409616532085	30.894485579085305	20.907872923610267
13	29.267792230555003	20.99855530535639	24.9966808232398	24.736971640848807
14	20.985239616143954	21.35590231675996	27.858269666954605	29.800588400141486
15	24.50923590578421	29.067781755740498	22.63691696343948	23.786065375035818
16	22.644597825653427	27.978023523583413	27.14096936644184	22.236409284321322
17	22.43510717635198	27.7961027830741	27.59236721106365	22.176422829510273
18	22.008697828901415	27.117872007464577	29.26997328306568	21.603456880568324
19	23.613897944091423	27.06308596753563	27.29229004103839	22.03072604733455
20	23.61818433725329	26.95091589240206	27.63284953166263	21.79805023868202
21	23.388302620964886	26.500039100078883	27.240788782423543	22.870869496532688
22	23.026697011177642	27.07455050093637	26.91509485458989	22.983657633296104
23	21.586076485490306	26.56786228056226	28.864043464273024	22.982017769674414
24	22.891267777145003	27.320706388093548	27.216726984518562	22.57129885024289
25	22.99548794204076	26.391419059810357	27.231584510682875	23.38150848746601
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	143.0
1	143.0
2	250.0
3	357.0
4	357.0
5	357.0
6	743.5
7	1130.0
8	1130.0
9	1130.0
10	1869.5
11	2609.0
12	2609.0
13	2609.0
14	4796.5
15	6984.0
16	6984.0
17	6984.0
18	12580.0
19	18176.0
20	18176.0
21	18176.0
22	28898.5
23	39621.0
24	39621.0
25	39621.0
26	56604.0
27	73587.0
28	73587.0
29	73587.0
30	95456.0
31	117325.0
32	117325.0
33	117325.0
34	138639.0
35	159953.0
36	159953.0
37	159953.0
38	180158.0
39	200363.0
40	200363.0
41	200363.0
42	218275.0
43	236187.0
44	236187.0
45	236187.0
46	256177.0
47	276167.0
48	276167.0
49	276167.0
50	266289.0
51	256411.0
52	256411.0
53	256411.0
54	227371.5
55	198332.0
56	198332.0
57	198332.0
58	170206.0
59	142080.0
60	142080.0
61	142080.0
62	116283.0
63	90486.0
64	90486.0
65	90486.0
66	69648.0
67	48810.0
68	48810.0
69	48810.0
70	35787.0
71	22764.0
72	22764.0
73	22764.0
74	15932.0
75	9100.0
76	9100.0
77	9100.0
78	6314.5
79	3529.0
80	3529.0
81	3529.0
82	2390.0
83	1251.0
84	1251.0
85	1251.0
86	825.0
87	399.0
88	399.0
89	399.0
90	250.0
91	101.0
92	101.0
93	101.0
94	60.0
95	19.0
96	19.0
97	19.0
98	15.5
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10971217736959414
2	0.045070664926102996
3	0.033002850103048646
4	0.029225099375831627
5	0.033842350264652425
6	0.020043066358290274
7	0.018993691156285547
8	0.02292884816380327
9	0.027808442853125247
10	0.03462938166615597
11	0.019885660077989566
12	0.019151097436586256
13	0.015898034310371605
14	0.020462816439092167
15	0.020882566519894053
16	0.021197379080495474
17	0.025447348648614615
18	0.019046159916385783
19	0.03184853738084345
20	0.02266650436330209
21	0.027755974093025014
22	0.034839256706556916
23	0.027021411451621704
24	0.03730528843126802
25	0.029225099375831627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1905896.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.17973371742927
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.94405604526554	61.43003668553491
2	10.826080868347688	15.844994302980787
3	2.8324782958806476	6.21840022358831
4	1.0300343336668418	3.0151055303019674
5	0.46457701257855316	1.6998811035868657
6	0.2575639656882076	1.1309077454560874
7	0.15148173885497976	0.7759775318722405
8	0.09979096225208972	0.5842140836011169
9	0.07134780294321928	0.46990918986375635
>10	0.2930209014749803	3.6761483161702877
>50	0.01755873411882072	0.8770283309444457
>100	0.00909888240304929	1.3732204417068725
>500	0.0016007510890048177	0.7812342381864218
>1k	0.001164182610185316	1.3993741148975785
>5k	1.455228262731645E-4	0.7235681613082909
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7580	0.3977132015597913	No Hit
CCCATGTACTCTGCGTTGATACCAC	6009	0.31528477944232003	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3269	0.17152037676767254	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2265	0.11884174162703526	No Hit
GTATCAACGCAGAGTACATGGGAAG	2045	0.10729861440498328	No Hit
GCGTTGATACCACTGCTTCCCATGT	2042	0.10714120812468256	No Hit
CATGTACTCTGCGTTGATACCACTG	2033	0.10666898928378044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.246876010023632E-5
7	0.0	0.0	0.0	1.0493752020047264E-4	5.246876010023632E-5
8	0.0	0.0	0.0	1.0493752020047264E-4	5.246876010023632E-5
9	0.0	0.0	0.0	1.0493752020047264E-4	5.246876010023632E-5
10	0.0	0.0	0.0	1.5740628030070896E-4	5.246876010023632E-5
11	0.0	0.0	0.0	1.5740628030070896E-4	5.246876010023632E-5
12	0.0	0.0	0.0	2.0987504040094528E-4	2.0987504040094528E-4
13	0.0	0.0	0.0	2.0987504040094528E-4	2.623438005011816E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	465	0.0	14.9151125	4
GTCCTAC	930	0.0	12.988423	1
GTAGGAC	940	0.0	12.937154	3
GGCGTGC	120	7.421477E-10	12.66497	8
TCCAACG	140	5.2750693E-11	12.213611	18
TGGCGAG	680	0.0	11.874344	18
TGTAGGA	1110	0.0	11.64083	2
GACTGCG	75	2.0768662E-4	11.398473	7
TAGGACA	530	0.0	11.114052	4
GCGAATT	60	0.0058823484	11.081849	13
CTAGGAC	320	0.0	10.985209	3
CTGTAGG	1120	0.0	10.954873	1
TATGGCG	755	0.0	10.946128	16
AAGGCGT	165	8.0035534E-11	10.938502	6
CTAGCAC	200	0.0	10.92583	3
TCCTACA	1140	0.0	10.917783	2
AGGACCT	845	0.0	10.793719	5
CCTACAG	1100	0.0	10.709905	3
ACACCGT	80	3.7743596E-4	10.686628	6
CGGTTTC	80	3.776191E-4	10.686068	13
>>END_MODULE