FastQCFastQC Report
Thu 26 May 2016
SRR1513063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1905896
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC86220.45238564958423755No Hit
CCCATGTACTCTGCGTTGATACCAC79020.41460814231206744No Hit
GAGTACATGGGAAGCAGTGGTATCA33690.17676725277769614No Hit
GTATCAACGCAGAGTACTTTTTTTT33540.17598022137619262No Hit
TATCAACGCAGAGTACTTTTTTTTT23930.12555774291986552No Hit
CATGTACTCTGCGTTGATACCACTG22720.11920902294773691No Hit
GTATCAACGCAGAGTACATGGGAAG22710.11915655418763668No Hit
GCGTTGATACCACTGCTTCCCATGT21640.1135423968569114No Hit
ACGCAGAGTACATGGGAAGCAGTGG20680.1085053958872887No Hit
GGTATCAACGCAGAGTACTTTTTTT20530.10771836448578516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1750.014.09997218
AGGACGG853.852472E-612.3201375
GTAGGAC8800.012.1164993
AGCCGTG550.00309776712.0757947
TAGGACG5800.011.8180634
CTAGGAC3050.011.5489823
CCAACGA1406.9667294E-1011.51887419
GTCCTAA4150.011.4790761
GTCCTAC9500.011.332871
TAGGACC5100.011.2001264
AGGACGT5800.011.1615045
CTAGTAC1451.1750672E-911.1615043
GGACGTG5800.011.161216
TAGGACA5050.011.12254
TGTGCGG600.00594420611.06628310
GACGTGG3650.010.9178417
GACCGCT700.001507316510.843577
TAAACTG2950.010.649615
CTAGTGC909.314891E-510.5778953
CTACACT3700.010.5493084
Produced by FastQC (version 0.11.5)