##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1905896 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.390283625129598 31.0 31.0 34.0 26.0 34.0 2 30.543024907969794 31.0 31.0 34.0 27.0 34.0 3 30.54551455063655 31.0 31.0 34.0 26.0 34.0 4 33.7646052040615 37.0 35.0 37.0 28.0 37.0 5 33.66600853351914 37.0 35.0 37.0 28.0 37.0 6 33.61645283897967 37.0 35.0 37.0 28.0 37.0 7 33.52605965907898 37.0 35.0 37.0 28.0 37.0 8 33.521103459999914 37.0 35.0 37.0 28.0 37.0 9 35.06505706502349 39.0 35.0 39.0 28.0 39.0 10 34.96941333629957 39.0 35.0 39.0 27.0 39.0 11 34.975535915915664 39.0 35.0 39.0 27.0 39.0 12 34.888294534434195 39.0 35.0 39.0 27.0 39.0 13 34.85408647691165 39.0 35.0 39.0 27.0 39.0 14 36.02007926980276 40.0 36.0 41.0 27.0 41.0 15 36.008166237822 40.0 36.0 41.0 27.0 41.0 16 35.96513031141259 40.0 36.0 41.0 27.0 41.0 17 35.87265412173592 40.0 36.0 41.0 27.0 41.0 18 35.799421374513614 40.0 36.0 41.0 27.0 41.0 19 35.87339655469134 40.0 36.0 41.0 27.0 41.0 20 35.76650404848953 40.0 36.0 41.0 27.0 41.0 21 35.78892867186877 40.0 36.0 41.0 27.0 41.0 22 35.765620999257045 40.0 36.0 41.0 27.0 41.0 23 35.699939031300765 40.0 36.0 41.0 27.0 41.0 24 35.56699053883318 40.0 36.0 41.0 26.0 41.0 25 35.54398980846804 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.581095462791275 1101 2 0.5032422907505456 1101 3 0.5421121893522844 1101 4 0.7574864105766395 1101 5 0.5609508523439715 1101 6 0.5547706846623726 1101 7 0.4914051973443918 1101 8 0.553223110821456 1101 9 0.6153467094670404 1101 10 0.5087528802909702 1101 11 0.5973229353061882 1101 12 0.6176472751624615 1101 13 0.6551589631889669 1101 14 0.6710057055677225 1101 15 0.6807262706873871 1101 16 0.6137226696051243 1101 17 0.7888677692335406 1101 18 0.6791063779230342 1101 19 0.6954581505492285 1101 20 0.8543815967480484 1101 21 0.8007469300710568 1101 22 0.8608878540650906 1101 23 0.7967370015402437 1101 24 0.906205077591089 1101 25 0.8695489786967343 1102 1 0.8500295223989092 1102 2 0.738206198460567 1102 3 0.7262988949897675 1102 4 1.0357876372716532 1102 5 0.8891223239351334 1102 6 0.8000831154670323 1102 7 0.6777317550699138 1102 8 0.7346517455143413 1102 9 0.8171783378271655 1102 10 0.7536260037133218 1102 11 0.7531300297661332 1102 12 0.7182805509252717 1102 13 0.8049486093516691 1102 14 0.8494265018123528 1102 15 0.8140294902082204 1102 16 0.8839250521391122 1102 17 0.8054781939268665 1102 18 0.7821345874797387 1102 19 0.8661237543754581 1102 20 0.9678522136383592 1102 21 0.9849920919327957 1102 22 1.0156374222861615 1102 23 0.9233921541021317 1102 24 1.0992718151137808 1102 25 1.1665048265334121 1103 1 0.05926991214719379 1103 2 0.1689608811301433 1103 3 0.37462392769366204 1103 4 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13 0.25267348511208354 2211 14 0.32537489914277984 2211 15 0.3265891209909526 2211 16 0.1893203829243717 2211 17 0.32259937920205317 2211 18 0.31705177515112126 2211 19 0.14578558645585105 2211 20 0.26120208412544343 2211 21 0.26938861238065215 2211 22 0.16430474706405107 2211 23 0.0903988448995392 2211 24 -3.2404033479374874 2211 25 -2.2944983712121356 2212 1 -0.01804508609841804 2212 2 -0.1531884204241436 2212 3 -0.473219898267466 2212 4 0.24269264918326883 2212 5 -0.00393348565964402 2212 6 -0.06121711373280192 2212 7 -0.05298395810287104 2212 8 -0.17210411714641083 2212 9 -0.1962500626289767 2212 10 -0.2195742130668208 2212 11 -0.2694333953600392 2212 12 -0.34185448503539106 2212 13 -0.25347250864673043 2212 14 -0.34538409783312574 2212 15 -0.4128967480918604 2212 16 -0.3011663455282445 2212 17 -0.3826990464168176 2212 18 -0.413996338690815 2212 19 -0.3256538427522031 2212 20 -0.6035674704497538 2212 21 -0.39481698347733385 2212 22 -0.33356997533602595 2212 23 -0.4459532150450869 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10 -0.4866753032805988 2214 11 -0.45026615899970324 2214 12 -0.4564708541763238 2214 13 -0.5163557240149217 2214 14 -0.6159757396988397 2214 15 -0.5552837559921855 2214 16 -0.6905074198419996 2214 17 -0.6497221785497729 2214 18 -0.6053448764199558 2214 19 -0.516980106743965 2214 20 -0.6369673679905645 2214 21 -0.6110307222612263 2214 22 -0.6387724350419788 2214 23 -0.6656048195251927 2214 24 -0.4699177156321639 2214 25 -0.5458680491857706 2215 1 -0.20539168037065636 2215 2 -0.17686319000355155 2215 3 -0.23640466305199226 2215 4 0.04259169662572759 2215 5 -0.2098018223818343 2215 6 -0.34495546685322864 2215 7 -0.5465188413750326 2215 8 -0.519388256331311 2215 9 -0.5649559443896806 2215 10 -0.7442958436782021 2215 11 -0.7695143587658393 2215 12 -0.8134367490994237 2215 13 -0.8797175307606437 2215 14 -0.9151514811587731 2215 15 -0.8605712105976266 2215 16 -1.0755452450383913 2215 17 -0.9179733970001109 2215 18 -1.000148324202982 2215 19 -0.9656193305273604 2215 20 -0.8647991503816996 2215 21 -0.7912193766381534 2215 22 -0.90916410200726 2215 23 -0.8706235828161155 2215 24 -0.9527667373503022 2215 25 -0.8837129335477982 2216 1 -1.1421482153928615 2216 2 -0.7568475327335165 2216 3 -2.3093656224291372 2216 4 -0.775460425906239 2216 5 -0.8543514602404088 2216 6 -0.8644343034363473 2216 7 -0.7876943457947831 2216 8 -0.9627481645171443 2216 9 -0.8765048730490221 2216 10 -0.9181318810270227 2216 11 -0.8888082918213129 2216 12 -0.8659299753905145 2216 13 -0.84639342093773 2216 14 -0.9972291687029653 2216 15 -1.0010922171941843 2216 16 -0.8692903224797703 2216 17 -1.050810105168452 2216 18 -1.1166438400706156 2216 19 -0.9642556128460171 2216 20 -0.8715769861417186 2216 21 -1.0898657078372835 2216 22 -1.1381637473044819 2216 23 -1.1343076509660932 2216 24 -1.263420242665994 2216 25 -1.1417132971233102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 56645.0 3 1.0 4 897.0 5 7505.0 6 8447.0 7 6458.0 8 5204.0 9 4514.0 10 3927.0 11 3565.0 12 3248.0 13 3078.0 14 2927.0 15 2919.0 16 3186.0 17 3291.0 18 3514.0 19 4123.0 20 5209.0 21 6869.0 22 8712.0 23 10467.0 24 12085.0 25 13341.0 26 14519.0 27 15816.0 28 17629.0 29 21360.0 30 26972.0 31 34066.0 32 43520.0 33 56967.0 34 75504.0 35 103499.0 36 152296.0 37 254597.0 38 909019.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.27356230241876 18.706968421694487 13.69031035827715 27.329158917609607 2 13.665540664701675 19.5215559198057 39.850546602725714 26.962356812766906 3 17.634750269689427 23.408989787480532 28.745849721076073 30.210410221753968 4 10.402298971192552 16.464172231853155 36.824936932550365 36.30859186440393 5 13.060846219322633 37.5890421598293 35.46877082723276 13.881340793615307 6 33.79170703964275 35.99649281712095 17.064603783123395 13.147196360112904 7 29.125100019676005 30.513805994621894 20.768098642355874 19.59299534334623 8 25.766036888175563 37.20502423207367 19.166746268092705 17.862192611658056 9 25.940625654424153 15.381979015389168 19.138813237383488 39.53858209280318 10 14.331198493894037 29.72880228266561 33.70583710506957 22.23416211837078 11 35.20433408104102 22.51151076305537 22.507522988731914 19.776632167171698 12 23.09253195642592 25.005509728606512 30.76511764582415 21.13684066914342 13 29.02426187736357 20.898104522793425 25.1256331506753 24.952000449167702 14 20.982253183990665 21.496560875571856 27.101895571717378 30.4192903687201 15 24.338473872656223 29.19466749497349 22.63365891947934 23.833199712890945 16 22.687601931904766 28.086083526964593 26.69167605961954 22.534638481511102 17 22.539774173487036 28.07664351371989 26.95380869035842 22.429773622434652 18 22.43667790747674 27.52859161207707 28.084436807063913 21.950293673382276 19 23.382668619084356 27.11729726609039 27.288343751476123 22.21169036334913 20 23.464764079467084 26.93620218521892 27.572228495153983 22.02680524016001 21 23.10440863509866 26.499819507465254 27.19970029844231 23.196071558993776 22 22.90219403367235 27.14859572610468 26.715466111477227 23.233744128745744 23 22.431613493922804 26.855729198093286 27.020008972162685 23.69264833582123 24 22.682712346764664 27.356272023808277 27.326941925355687 22.634073704071376 25 22.922761787631643 26.456795124183063 26.96715875367806 23.653284334507234 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 83.0 1 83.0 2 215.5 3 348.0 4 348.0 5 348.0 6 840.5 7 1333.0 8 1333.0 9 1333.0 10 1911.0 11 2489.0 12 2489.0 13 2489.0 14 4631.5 15 6774.0 16 6774.0 17 6774.0 18 12280.0 19 17786.0 20 17786.0 21 17786.0 22 28434.5 23 39083.0 24 39083.0 25 39083.0 26 55576.0 27 72069.0 28 72069.0 29 72069.0 30 93697.0 31 115325.0 32 115325.0 33 115325.0 34 136046.0 35 156767.0 36 156767.0 37 156767.0 38 176175.0 39 195583.0 40 195583.0 41 195583.0 42 213092.0 43 230601.0 44 230601.0 45 230601.0 46 253857.0 47 277113.0 48 277113.0 49 277113.0 50 269269.0 51 261425.0 52 261425.0 53 261425.0 54 231480.5 55 201536.0 56 201536.0 57 201536.0 58 173894.0 59 146252.0 60 146252.0 61 146252.0 62 119677.0 63 93102.0 64 93102.0 65 93102.0 66 71717.5 67 50333.0 68 50333.0 69 50333.0 70 36872.5 71 23412.0 72 23412.0 73 23412.0 74 16310.0 75 9208.0 76 9208.0 77 9208.0 78 6342.0 79 3476.0 80 3476.0 81 3476.0 82 2391.0 83 1306.0 84 1306.0 85 1306.0 86 840.0 87 374.0 88 374.0 89 374.0 90 240.0 91 106.0 92 106.0 93 106.0 94 58.0 95 10.0 96 10.0 97 10.0 98 6.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18437522299223041 2 0.06411682484248878 3 0.0 4 0.0 5 0.017734440913879875 6 0.3639233200552391 7 0.0011018439621049627 8 0.0299071932571347 9 0.03121891225964061 10 0.0028333130454127613 11 0.0037252819671167786 12 0.009234501777641591 13 0.007817845254935212 14 0.0017314690833077986 15 0.0 16 0.0111233771412501 17 0.02366341080520658 18 0.06448410616319043 19 0.029697318216733756 20 0.0 21 0.0 22 0.0 23 5.246876010023632E-5 24 2.0987504040094528E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1905896.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.40832571175038 #Duplication Level Percentage of deduplicated Percentage of total 1 80.60589852471736 55.14114560567166 2 12.66707301524406 17.330665132826798 3 3.57293106455629 7.332546960293931 4 1.3553765109798515 3.70876151300662 5 0.6228823958659997 2.1305170908258377 6 0.33885872896631664 1.390845498083852 7 0.202725783523516 0.9707692000612537 8 0.12427201203690014 0.6800992221019864 9 0.08312508505593069 0.5117803103990755 >10 0.39035971893548793 4.5827191031316445 >50 0.021891727491520945 1.0344240694717706 >100 0.011070424070630936 1.5026939492755216 >500 0.001998048314359727 0.9984703367980806 >1k 0.0013832642176336539 1.8158880836981643 >5k 1.536960241815171E-4 0.8686739243537858 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 8622 0.45238564958423755 No Hit CCCATGTACTCTGCGTTGATACCAC 7902 0.41460814231206744 No Hit GAGTACATGGGAAGCAGTGGTATCA 3369 0.17676725277769614 No Hit GTATCAACGCAGAGTACTTTTTTTT 3354 0.17598022137619262 No Hit TATCAACGCAGAGTACTTTTTTTTT 2393 0.12555774291986552 No Hit CATGTACTCTGCGTTGATACCACTG 2272 0.11920902294773691 No Hit GTATCAACGCAGAGTACATGGGAAG 2271 0.11915655418763668 No Hit GCGTTGATACCACTGCTTCCCATGT 2164 0.1135423968569114 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2068 0.1085053958872887 No Hit GGTATCAACGCAGAGTACTTTTTTT 2053 0.10771836448578516 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 5.246876010023632E-5 0.0 0.0 0.0 0.0 11 5.246876010023632E-5 0.0 0.0 0.0 0.0 12 5.246876010023632E-5 0.0 0.0 5.246876010023632E-5 3.148125606014179E-4 13 5.246876010023632E-5 0.0 0.0 5.246876010023632E-5 3.148125606014179E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAACG 175 0.0 14.099972 18 AGGACGG 85 3.852472E-6 12.320137 5 GTAGGAC 880 0.0 12.116499 3 AGCCGTG 55 0.003097767 12.075794 7 TAGGACG 580 0.0 11.818063 4 CTAGGAC 305 0.0 11.548982 3 CCAACGA 140 6.9667294E-10 11.518874 19 GTCCTAA 415 0.0 11.479076 1 GTCCTAC 950 0.0 11.33287 1 TAGGACC 510 0.0 11.200126 4 AGGACGT 580 0.0 11.161504 5 CTAGTAC 145 1.1750672E-9 11.161504 3 GGACGTG 580 0.0 11.16121 6 TAGGACA 505 0.0 11.1225 4 TGTGCGG 60 0.005944206 11.066283 10 GACGTGG 365 0.0 10.917841 7 GACCGCT 70 0.0015073165 10.84357 7 TAAACTG 295 0.0 10.64961 5 CTAGTGC 90 9.314891E-5 10.577895 3 CTACACT 370 0.0 10.549308 4 >>END_MODULE