FastQCFastQC Report
Thu 26 May 2016
SRR1513062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625333
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC81200.499589930186614No Hit
CCCATGTACTCTGCGTTGATACCAC66520.4092699772908075No Hit
GAGTACATGGGAAGCAGTGGTATCA36460.22432326175620626No Hit
GCGTTGATACCACTGCTTCCCATGT23080.1420016698116632No Hit
GTATCAACGCAGAGTACATGGGAAG22680.13954063567281288No Hit
CATGTACTCTGCGTTGATACCACTG22160.1363412912923075No Hit
GCTTCCCATGTACTCTGCGTTGATA17230.10600904553097734No Hit
TATCAACGCAGAGTACATGGGAAGC17050.10490158016849471No Hit
ACGCAGAGTACATGGGAAGCAGTGG17000.1045939509011384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG1800.014.2525854
GTAGGAC4000.013.3015953
ACACCGA957.393646E-812.9991596
GCGTTTA1403.6379788E-1212.89083113
TGTAGGA5350.011.89862252
GTACCGT802.8684504E-511.874236
TAAGTAG1052.7146416E-711.7636785
TAGGACC2200.011.6612064
GTCTACG752.0556798E-411.4108511
TAAGGTG1751.8189894E-1211.4017185
GTATTAT1553.074092E-1011.0427581
GTCCTAC2950.010.9595751
GTTGGAC1658.0035534E-1110.9407053
CGTTTAT1658.0035534E-1110.93767514
GTAGAAC3000.010.7679583
CTGTAGG5300.010.5855381
GTATTAG1355.607035E-810.5656021
CTAGTAC909.503207E-510.5568213
TTAGACT1451.4388206E-810.4846614
TACACCG1201.5204423E-610.2932185
Produced by FastQC (version 0.11.5)