##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513062_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1625333
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.45268692630987	31.0	28.0	34.0	16.0	34.0
2	28.142585550161105	31.0	28.0	34.0	15.0	34.0
3	28.468755018202422	31.0	28.0	34.0	16.0	34.0
4	31.253749231695906	35.0	32.0	37.0	12.0	37.0
5	31.451325974431086	35.0	32.0	37.0	16.0	37.0
6	31.92799936997526	35.0	32.0	37.0	18.0	37.0
7	31.891171224604435	35.0	32.0	37.0	17.0	37.0
8	31.873109079800876	35.0	32.0	37.0	17.0	37.0
9	33.142592318004986	38.0	33.0	39.0	17.0	39.0
10	32.59474704568233	37.0	32.0	39.0	10.0	39.0
11	33.01642678761829	38.0	33.0	39.0	17.0	39.0
12	32.85992531991906	38.0	33.0	39.0	13.0	39.0
13	32.94215585360046	38.0	33.0	39.0	15.0	39.0
14	33.184251473390376	39.0	32.0	41.0	10.0	41.0
15	33.69350403886465	39.0	33.0	41.0	10.0	41.0
16	33.23535177099093	38.0	32.0	41.0	8.0	41.0
17	32.74609449263628	38.0	32.0	41.0	8.0	41.0
18	32.11035830811286	38.0	30.0	41.0	2.0	41.0
19	33.02551477143453	39.0	32.0	41.0	2.0	41.0
20	33.2948466560391	39.0	32.0	41.0	2.0	41.0
21	33.32747381613491	39.0	33.0	41.0	2.0	41.0
22	32.87912569301183	39.0	32.0	41.0	2.0	41.0
23	31.13213599920755	38.0	28.0	40.0	2.0	41.0
24	32.186746346748635	38.0	32.0	41.0	2.0	41.0
25	31.91430925231937	38.0	31.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7877593394378977
1101	2	1.1391557661234266
1101	3	0.8534107698535252
1101	4	0.9288202738333133
1101	5	1.1426602595654423
1101	6	0.8958710007671371
1101	7	0.8409007275701512
1101	8	0.8528726893313383
1101	9	1.021024290865192
1101	10	1.4011056899287624
1101	11	1.0225326289665375
1101	12	1.0414942246663728
1101	13	0.9921495745772972
1101	14	0.254245360495581
1101	15	0.8066330885434212
1101	16	1.4127273920395922
1101	17	2.0775275662681594
1101	18	-4.070632411899652
1101	19	-0.5459755320711182
1101	20	0.47652281915883066
1101	21	0.8953965795198471
1101	22	1.4922684780286772
1101	23	3.404049520470778
1101	24	2.384437165703808
1101	25	2.5765092921568034
1102	1	1.133348844969202
1102	2	1.3721514473169876
1102	3	1.2012511356818365
1102	4	1.7870442512923752
1102	5	1.6258769620297038
1102	6	1.2341213067788175
1102	7	1.1047405391131448
1102	8	1.1178945143157542
1102	9	1.2408233624372826
1102	10	1.6685266465328397
1102	11	1.2310852514289508
1102	12	1.3243363226363059
1102	13	1.2126073218693847
1102	14	2.181968402598166
1102	15	1.5530868172669443
1102	16	1.8903735270542583
1102	17	1.6192523174171427
1102	18	2.1657314764118567
1102	19	1.7910650462332285
1102	20	1.626568778649812
1102	21	1.6433551928827441
1102	22	1.9847320453166049
1102	23	3.7367177114220738
1102	24	2.606891263950885
1102	25	2.822539454516214
1103	1	0.6328255006254899
1103	2	0.852549695027065
1103	3	0.6471522296917236
1103	4	1.0468395051167256
1103	5	1.059653053111294
1103	6	0.5484160569369507
1103	7	0.5324585797344099
1103	8	0.49816625981203444
1103	9	0.6833693366214604
1103	10	0.9231760714163002
1103	11	0.6108598900777196
1103	12	0.8089302271037084
1103	13	0.5238074530568682
1103	14	1.338544409344955
1103	15	0.9593674179182017
1103	16	1.1984813819046778
1103	17	1.6005154654481615
1103	18	1.5981310359967438
1103	19	1.230829845820928
1103	20	1.2250666819927503
1103	21	1.0536866755355447
1103	22	1.5168064473593788
1103	23	3.0764323786509706
1103	24	2.048278103035379
1103	25	2.135839053925867
1104	1	0.7590584767843112
1104	2	1.0755056792919113
1104	3	0.7873142004061577
1104	4	1.4406222216654818
1104	5	1.2641353061196874
1104	6	0.8076645485269296
1104	7	0.7732358369187864
1104	8	0.7617060577419004
1104	9	0.8706113026917564
1104	10	1.292905219278559
1104	11	0.8296180438562644
1104	12	0.9914990247077284
1104	13	0.7971177404568834
1104	14	1.7145991727745766
1104	15	1.3189482715665335
1104	16	1.6704322276504797
1104	17	2.1032577879439174
1104	18	2.656854471064264
1104	19	1.9173561520172342
1104	20	1.2520042694605564
1104	21	1.332631731084227
1104	22	1.7724291994133523
1104	23	3.2881299063941043
1104	24	2.3472493068545646
1104	25	2.4731765557516887
1105	1	0.5262157668858976
1105	2	0.9452480942597887
1105	3	0.6597335582366561
1105	4	0.8223569325779074
1105	5	0.8429323048769
1105	6	0.5849624716471311
1105	7	0.45266953319598713
1105	8	0.5159012436346977
1105	9	0.7425428194508541
1105	10	1.3262758053679775
1105	11	0.7802301638317815
1105	12	0.731439234807091
1105	13	0.7808402955322222
1105	14	1.6353991950334859
1105	15	0.9191269611351487
1105	16	1.245078249179791
1105	17	1.840607463025684
1105	18	1.6836430670836293
1105	19	1.3207645473354432
1105	20	1.0495134358026164
1105	21	0.8988767151010961
1105	22	1.4322572802626539
1105	23	2.8406508823235725
1105	24	1.8818605179246646
1105	25	1.8796620905528734
1106	1	-0.5424778966754928
1106	2	0.025098690474465002
1106	3	-0.17763726061281915
1106	4	-2.460079594675996
1106	5	-0.46242599898016934
1106	6	-0.29757013219264294
1106	7	-0.3974411348257689
1106	8	-0.3537843246137129
1106	9	-0.2604352417580671
1106	10	-0.16586633477709256
1106	11	-0.17950873943991041
1106	12	-0.21520696473020706
1106	13	-0.27419296746491284
1106	14	0.41065978712500595
1106	15	-0.09365544965172035
1106	16	-1.1373107874490174
1106	17	-5.9583511857056415
1106	18	-7.212100849695897
1106	19	-3.1118608079640637
1106	20	-1.5493990523540688
1106	21	-1.2370450528958514
1106	22	-1.6538817945073063
1106	23	-5.64247977479177
1106	24	-2.358483198408436
1106	25	-0.8070421774225593
1107	1	0.751112294617684
1107	2	0.957902122731884
1107	3	0.7828703151946286
1107	4	1.0674707653947415
1107	5	1.0892592582133211
1107	6	0.6395586804932591
1107	7	0.6879130593755889
1107	8	0.6115821661422096
1107	9	0.6066998125928009
1107	10	1.0367201670904294
1107	11	0.7879519317174299
1107	12	0.8286913676261278
1107	13	0.5704795946688961
1107	14	1.4610542275881429
1107	15	0.9480338813878504
1107	16	-5.970278352377562
1107	17	-1.0664944228073132
1107	18	0.7101508466300288
1107	19	0.8485893573857126
1107	20	0.6598698736922302
1107	21	0.6339732448121183
1107	22	0.9002721123661956
1107	23	2.765347735411602
1107	24	1.7995085439775238
1107	25	1.421817952484293
1108	1	0.40430638559202237
1108	2	0.5572746385567235
1108	3	0.4340298975581156
1108	4	0.8749435205033897
1108	5	0.6115725151825195
1108	6	0.2872649263697937
1108	7	0.40352818088084064
1108	8	0.1734380050792801
1108	9	0.34925741889130535
1108	10	0.9813735029132502
1108	11	0.4094563511398519
1108	12	0.5205026327270161
1108	13	0.2019882600092089
1108	14	0.8047521488694613
1108	15	0.5770111464824055
1108	16	0.7601366532668052
1108	17	0.8688169880253156
1108	18	0.6728533333673852
1108	19	0.6823564646386799
1108	20	0.549286730906978
1108	21	0.5827834444336588
1108	22	0.9998712633033691
1108	23	2.7612440021886755
1108	24	1.5483766136637058
1108	25	1.7366929892191045
1109	1	0.09006615440953425
1109	2	0.4746680036083788
1109	3	0.43240812828020125
1109	4	0.4393283702379698
1109	5	0.1855185670160644
1109	6	0.02153852532186562
1109	7	0.03968405294251909
1109	8	-0.06751257567256275
1109	9	0.007617285304441168
1109	10	0.5765983255355707
1109	11	0.0917682796071091
1109	12	0.17224958065474283
1109	13	0.10049201216934733
1109	14	-1.1597430634452053
1109	15	-0.3489129230265249
1109	16	-0.671943055207791
1109	17	0.588884832674907
1109	18	1.2999114530382698
1109	19	0.46559043254669774
1109	20	0.24166292226752262
1109	21	0.03341622391767629
1109	22	-0.03913758200452122
1109	23	-4.608146591915155
1109	24	-2.0684703773943944
1109	25	-0.8737519362122654
1110	1	0.279083876548782
1110	2	0.6057857242871485
1110	3	0.44771407280772024
1110	4	1.0933913716248291
1110	5	0.6311292236076262
1110	6	0.38861070774196094
1110	7	0.2826288329157478
1110	8	0.2993122198830598
1110	9	0.194152495161525
1110	10	0.5829305350966862
1110	11	0.22556955165248382
1110	12	0.2807919086962798
1110	13	0.02035744563733033
1110	14	0.8731540245133473
1110	15	0.5715679970380165
1110	16	0.9552984411668035
1110	17	1.2408371472174693
1110	18	1.7411210078939234
1110	19	1.046014113848031
1110	20	0.7071211587449255
1110	21	0.5125515263510962
1110	22	0.7037452022243542
1110	23	2.15007864875799
1110	24	1.2417800228209188
1110	25	1.631884609563695
1111	1	0.6202697868619431
1111	2	0.9172199404676746
1111	3	0.7125466858653802
1111	4	1.3187444930329129
1111	5	0.9354570497725625
1111	6	0.48169047143098
1111	7	0.5370084234358217
1111	8	0.191819664284413
1111	9	0.5264147314049623
1111	10	0.622921847535622
1111	11	0.4981216915757827
1111	12	0.6182364032164784
1111	13	0.6670034182074644
1111	14	-0.20399990253210376
1111	15	0.3448139190665742
1111	16	0.7361430677472427
1111	17	1.2810089652817709
1111	18	1.953969456535411
1111	19	0.989124854631477
1111	20	0.7587390714244577
1111	21	0.6712529455911351
1111	22	1.1026629254312112
1111	23	0.8116617529656303
1111	24	0.8794769923061523
1111	25	0.2710209658422009
1112	1	0.5342710928177148
1112	2	0.9450330613253612
1112	3	0.7251827060260005
1112	4	1.2546280903095202
1112	5	1.1240341084055707
1112	6	0.39048620523153943
1112	7	0.46598911162931955
1112	8	0.34296941523158253
1112	9	0.484037944044573
1112	10	0.9983490787413558
1112	11	0.5329672162438044
1112	12	0.6336510557621367
1112	13	0.4453129355194889
1112	14	0.864129967642917
1112	15	0.7049404936442372
1112	16	1.0840649815576384
1112	17	1.2330724507236113
1112	18	1.4256540004271727
1112	19	0.9564071571925723
1112	20	0.749632289550938
1112	21	0.887092700066809
1112	22	0.6276025380131145
1112	23	2.521206093756355
1112	24	1.492752867061867
1112	25	1.5421422221554373
1113	1	0.28644898368625604
1113	2	0.7833177953093404
1113	3	0.47527430517908087
1113	4	0.7888633192637364
1113	5	0.8655125485778932
1113	6	0.27262091312231007
1113	7	0.17701587372117444
1113	8	0.12410079032519761
1113	9	0.428820213185233
1113	10	0.6138263878442842
1113	11	0.3073031496781127
1113	12	0.3063533435938268
1113	13	0.25899130567323425
1113	14	0.8949305740824443
1113	15	0.4494726756255858
1113	16	0.9903997708213623
1113	17	1.277536724870373
1113	18	1.9143896700908556
1113	19	0.8845963325527251
1113	20	0.6918152826051198
1113	21	0.5890898309062109
1113	22	0.7757844203775264
1113	23	0.7075121477434472
1113	24	0.78692800522483
1113	25	1.1490561567994213
1114	1	-0.7483346252406733
1114	2	-3.466425926879001
1114	3	-1.3755813407820732
1114	4	-0.2777341867570762
1114	5	0.015336355321636574
1114	6	-0.14301474628031485
1114	7	-0.3133449697782673
1114	8	-0.287959350499186
1114	9	-0.24700285999266214
1114	10	-0.1037940229381249
1114	11	-0.3013818648159585
1114	12	-0.41899945889537804
1114	13	-0.5166085869686086
1114	14	0.31864196873291206
1114	15	-0.3217724113280127
1114	16	0.06650136752075042
1114	17	0.28817444245331103
1114	18	0.747488392572194
1114	19	0.06602264788909906
1114	20	-0.21104801153958874
1114	21	-0.3428089254320028
1114	22	-0.251572955684928
1114	23	1.4028101642942516
1114	24	0.4692856019357947
1114	25	0.3400587618986428
1115	1	-0.2036759290122454
1115	2	-0.004135977832714133
1115	3	-0.21680921420662358
1115	4	-0.20594858656132686
1115	5	-0.051685559985092056
1115	6	-0.5877760749283532
1115	7	-0.6138223351383516
1115	8	-0.6586159940792236
1115	9	-0.8036570884256946
1115	10	-0.3777079431113961
1115	11	-0.5073390763860104
1115	12	-0.744718508877142
1115	13	-0.8255516542959285
1115	14	-0.05661795410019721
1115	15	-0.6114899116282047
1115	16	-0.1429480650556414
1115	17	0.3407716317296945
1115	18	0.7544411159562969
1115	19	-0.0686696021171258
1115	20	-0.5848108151038574
1115	21	-0.3964686887657223
1115	22	-0.15361948691305116
1115	23	1.602351150426415
1115	24	0.2935743683303613
1115	25	0.09256645933011143
1116	1	-0.2754710100596931
1116	2	-0.3356679734666592
1116	3	-0.1070653288937713
1116	4	0.4914611935377593
1116	5	-0.24097342378050257
1116	6	-0.3160991561934203
1116	7	-0.4766052871219486
1116	8	-0.3687805032210747
1116	9	-0.3107529478142439
1116	10	0.06340489084590217
1116	11	-0.37369512068582367
1116	12	-0.3160047269010562
1116	13	-0.3247561414034905
1116	14	0.16849043219365
1116	15	-0.46664127840062264
1116	16	0.016996101126473206
1116	17	0.21449165335594955
1116	18	0.8161002508381543
1116	19	-0.09300015736098288
1116	20	-0.37822194854395974
1116	21	-0.5416559972832715
1116	22	-0.1517742846429755
1116	23	1.156395701868675
1116	24	0.2253894434611894
1116	25	0.22244826301405496
1201	1	1.1996696599401417
1201	2	1.5952296232127523
1201	3	1.328389476360126
1201	4	1.9149937307584288
1201	5	1.7821233693874312
1201	6	1.146144682904513
1201	7	1.1682584544225918
1201	8	1.1214239260804568
1201	9	1.3490136287718286
1201	10	1.621920974948246
1201	11	1.267967476631732
1201	12	1.4118106152849563
1201	13	1.4204584427737714
1201	14	2.3184239815724297
1201	15	1.6347292136391758
1201	16	2.111317833115443
1201	17	2.434988409899738
1201	18	2.9974674411560613
1201	19	2.1372130950292956
1201	20	1.77264593263844
1201	21	1.6866837360263816
1201	22	1.974329988728499
1201	23	3.6876198638139783
1201	24	2.5582328364104896
1201	25	2.8681353541138357
1202	1	1.412397689158965
1202	2	1.6107995586489956
1202	3	1.2982386225254814
1202	4	1.997862479179954
1202	5	1.7721522514695316
1202	6	1.499154636213973
1202	7	1.335356841300026
1202	8	1.3273881407921841
1202	9	1.5261794611391863
1202	10	1.952921454157682
1202	11	1.509226599419435
1202	12	1.5927314421155074
1202	13	1.4502871254011325
1202	14	2.3711174300023714
1202	15	1.8700361019510012
1202	16	2.126190000761035
1202	17	2.5867734650412046
1202	18	3.206465893439102
1202	19	2.4414981339812485
1202	20	2.0663504805950055
1202	21	2.061808395237243
1202	22	2.3224835753520807
1202	23	4.062137740268604
1202	24	2.8542510269415686
1202	25	3.0096357005693086
1203	1	1.2719592967408033
1203	2	1.4845925180321302
1203	3	1.1325838893186493
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2211	15	-0.789088402075663
2211	16	-0.8412739963035776
2211	17	-0.29645433666213705
2211	18	-5.913715577916179
2211	19	-2.3981289867487057
2211	20	-1.581787743936296
2211	21	-1.2988955507467708
2211	22	-0.7872186241372674
2211	23	-5.509047908543909
2211	24	-2.869918134627042
2211	25	-2.091764305963906
2212	1	0.1291578131845874
2212	2	0.27009713798994994
2212	3	0.06475045276404146
2212	4	0.6667414524869066
2212	5	0.4022410613884517
2212	6	0.1937550776425354
2212	7	0.08585033840476086
2212	8	-0.07731672720565186
2212	9	-0.3437829759658442
2212	10	-3.920361007702404
2212	11	-1.3826694074593995
2212	12	-0.36916878188484503
2212	13	-0.13667156323838014
2212	14	0.6590515333711409
2212	15	0.19706440830336192
2212	16	0.2915171249892978
2212	17	1.046911182616114
2212	18	1.5466616335986956
2212	19	0.9428450309543877
2212	20	0.5275915348597309
2212	21	0.32412978371080925
2212	22	0.44206611944212426
2212	23	1.8811814473296593
2212	24	1.0217784088915067
2212	25	0.7584243539305255
2213	1	-1.387435468756287
2213	2	-3.8498093369967386
2213	3	-1.7509057270632624
2213	4	-3.540014572237034
2213	5	-1.3764759791467718
2213	6	-1.196859085378044
2213	7	-1.047808480494698
2213	8	-0.8872035248999346
2213	9	-0.7685884022685983
2213	10	-0.6342184515063742
2213	11	-0.8442088662181106
2213	12	-0.9380166185401677
2213	13	-1.118373070287479
2213	14	-6.256462969950849
2213	15	-2.9939720062624637
2213	16	-2.239038237651627
2213	17	-1.1191790551318732
2213	18	-0.11975871966657792
2213	19	-0.7614839733746166
2213	20	-0.8946076919384254
2213	21	-1.071546767901573
2213	22	-1.0627189350755515
2213	23	-5.864458759166162
2213	24	-3.582128343892254
2213	25	-2.888048780777904
2214	1	-0.17882597230604702
2214	2	0.03910299252873983
2214	3	-0.04885591820419677
2214	4	0.14322502348679578
2214	5	-0.03297349917729164
2214	6	-0.15209634701295371
2214	7	-0.4248283277315714
2214	8	-0.6279083898294999
2214	9	-0.4122601036847655
2214	10	-0.0563107527534612
2214	11	-0.41535623550539214
2214	12	-0.4681962386522329
2214	13	-0.41108368066669243
2214	14	0.20761405498350172
2214	15	-0.047644081731341714
2214	16	0.33894202732731316
2214	17	0.5093551819128095
2214	18	0.8817859684108669
2214	19	-0.15953815116935033
2214	20	-0.1628422379506489
2214	21	-0.293998010130629
2214	22	0.30949792572447166
2214	23	1.678052780359991
2214	24	0.764837564943619
2214	25	0.5581818900482283
2215	1	-2.839305613040416
2215	2	-4.743760502368545
2215	3	-2.8533474584728076
2215	4	-4.576081904830449
2215	5	-5.301208683549287
2215	6	-3.0131456188593617
2215	7	-2.254536190446604
2215	8	-2.641154690271744
2215	9	-2.3258655017876535
2215	10	-3.8575120142144748
2215	11	-2.749798026410488
2215	12	-2.517958349949712
2215	13	-2.346752648533869
2215	14	-1.5526311164547337
2215	15	-2.216738376961686
2215	16	-2.9367213675073423
2215	17	-7.329468548287629
2215	18	-3.8983556490043476
2215	19	-2.982867621173355
2215	20	-2.6762355128781223
2215	21	-2.6947348922078973
2215	22	-2.4903502706286424
2215	23	-7.395991315584954
2215	24	-4.830679953215228
2215	25	-4.598167167755339
2216	1	-1.0787817102940487
2216	2	-0.7024102700354256
2216	3	-0.9657308700005807
2216	4	-3.2675907762427876
2216	5	-1.517746104370385
2216	6	-1.2162943514343745
2216	7	-1.2959750243931154
2216	8	-0.9631020130966448
2216	9	-1.017832851389933
2216	10	-0.7008903796694099
2216	11	-1.420767496825988
2216	12	-2.2020844567666913
2216	13	-1.4194509755103901
2216	14	-6.835015006149945
2216	15	-3.6522413247233025
2216	16	-2.004724512161431
2216	17	-1.1095347322864555
2216	18	-0.3773380805701514
2216	19	-0.8204285172028776
2216	20	-1.4698021919208877
2216	21	-1.488261489568412
2216	22	-0.8694238352825039
2216	23	0.2483386030703265
2216	24	-0.47084348647880603
2216	25	-0.4479719629309109
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104611.0
3	1.0
4	844.0
5	4986.0
6	5851.0
7	5290.0
8	4906.0
9	4505.0
10	4312.0
11	4219.0
12	4055.0
13	4050.0
14	4228.0
15	4299.0
16	4413.0
17	4582.0
18	5166.0
19	6219.0
20	7616.0
21	9541.0
22	11832.0
23	14256.0
24	16473.0
25	19246.0
26	21513.0
27	24702.0
28	27448.0
29	33295.0
30	38629.0
31	43797.0
32	56075.0
33	71636.0
34	90822.0
35	117635.0
36	160322.0
37	215688.0
38	468270.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.50656085535939	17.23061383492081	11.934012669025556	25.32881264069424
2	14.714298992418362	18.647923805678136	40.790344352981656	25.847432848921848
3	20.29896841862964	22.93679109938718	27.828693853409707	28.935546628573466
4	11.496334754381463	15.217216038088274	37.230414738035414	36.056034469494854
5	13.34149432269635	37.26064021299813	35.31862016502924	14.079245299276282
6	34.88604790695528	34.44150089998615	16.916847433116413	13.755603759942156
7	30.153528811959358	30.43438395556532	20.11486507691825	19.297222155557073
8	27.04984162755305	35.63764679528877	18.997124249292447	18.315387327865736
9	26.78756607423208	15.363255187931149	18.64399582245302	39.20518291538375
10	15.068477974538947	28.727636642055792	33.27059570790937	22.933289675495892
11	36.05103466864573	22.524742878980046	21.586042696273815	19.838179756100413
12	23.8255529162718	24.530282704212873	29.93187776150447	21.712286618010857
13	29.54447837093759	20.358849584205597	24.660909418724717	25.435762626132096
14	21.82225345664091	20.543782477162907	26.944137433630278	30.689826632565904
15	25.386878589630847	27.878950863172946	22.0612642639076	24.672906283288604
16	23.628459850412124	27.297264365863832	26.009201242093837	23.065074541630203
17	23.564935532488537	27.103925284028147	26.787480299114975	22.543658884368345
18	23.379673076201197	26.360115862690282	27.913320824823217	22.346890236285304
19	24.122697423083313	26.69118235362904	26.772422621721926	22.413697601565723
20	24.25103232675065	26.231438118857582	26.627322350566473	22.8902072038253
21	23.861310337782037	25.99191085695436	26.88876935972439	23.258009445539216
22	23.837355300281153	26.096295785674545	26.090387162531453	23.975961751512852
23	22.68254481113932	25.7251788599123	28.036495115008847	23.555781213939532
24	23.43204036173907	26.968431846480627	26.14649903028669	23.45302876149361
25	23.76157799181463	25.73086746468905	26.102717173892977	24.40483736960335
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	117.0
1	117.0
2	208.0
3	299.0
4	299.0
5	299.0
6	593.0
7	887.0
8	887.0
9	887.0
10	1355.5
11	1824.0
12	1824.0
13	1824.0
14	3272.0
15	4720.0
16	4720.0
17	4720.0
18	8563.0
19	12406.0
20	12406.0
21	12406.0
22	19767.0
23	27128.0
24	27128.0
25	27128.0
26	39099.5
27	51071.0
28	51071.0
29	51071.0
30	67458.5
31	83846.0
32	83846.0
33	83846.0
34	101216.0
35	118586.0
36	118586.0
37	118586.0
38	136809.0
39	155032.0
40	155032.0
41	155032.0
42	173247.0
43	191462.0
44	191462.0
45	191462.0
46	215086.5
47	238711.0
48	238711.0
49	238711.0
50	233511.0
51	228311.0
52	228311.0
53	228311.0
54	206670.5
55	185030.0
56	185030.0
57	185030.0
58	161526.0
59	138022.0
60	138022.0
61	138022.0
62	115160.5
63	92299.0
64	92299.0
65	92299.0
66	72423.5
67	52548.0
68	52548.0
69	52548.0
70	39236.0
71	25924.0
72	25924.0
73	25924.0
74	18255.0
75	10586.0
76	10586.0
77	10586.0
78	7419.5
79	4253.0
80	4253.0
81	4253.0
82	2916.0
83	1579.0
84	1579.0
85	1579.0
86	1048.0
87	517.0
88	517.0
89	517.0
90	327.0
91	137.0
92	137.0
93	137.0
94	80.0
95	23.0
96	23.0
97	23.0
98	19.0
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10385564065948333
2	0.046144390103443414
3	0.03359311599530681
4	0.029286306252318754
5	0.03260870233976668
6	0.01894996286914743
7	0.018211652627492334
8	0.02251846237048039
9	0.028055789182893597
10	0.0355004174529158
11	0.019688273110802526
12	0.01925759213650372
13	0.015442989221285732
14	0.021226419447583973
15	0.020918790180227683
16	0.020611160912871393
17	0.024979496509330703
18	0.018826911162204914
19	0.03217802136546788
20	0.022333884810066616
21	0.027009849673882213
22	0.0362387276945709
23	0.02817884088983611
24	0.03851518427300744
25	0.02971698722661756
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1625333.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.4898658205496
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.97979239508982	63.301333329684006
2	10.135871760721304	15.100394548608554
3	2.6197253379066447	5.8542896672207965
4	0.9788234361386587	2.9164970567991193
5	0.43534673065507107	1.6214459775955592
6	0.23898925339549487	1.0681366450790242
7	0.1443128857639793	0.7524893247714585
8	0.09589403821489854	0.5714507231694758
9	0.07010083619702036	0.46996216940019353
>10	0.27639940488382503	3.480004922917796
>50	0.014592461380338708	0.7512183546701308
>100	0.0074670614256377036	1.124430636847754
>500	0.0016777488920514875	0.8287736346368998
>1k	8.388744460257438E-4	1.2365055177708644
>5k	1.6777488920514876E-4	0.9230674908283408
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8120	0.499589930186614	No Hit
CCCATGTACTCTGCGTTGATACCAC	6652	0.4092699772908075	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3646	0.22432326175620626	No Hit
GCGTTGATACCACTGCTTCCCATGT	2308	0.1420016698116632	No Hit
GTATCAACGCAGAGTACATGGGAAG	2268	0.13954063567281288	No Hit
CATGTACTCTGCGTTGATACCACTG	2216	0.1363412912923075	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1723	0.10600904553097734	No Hit
TATCAACGCAGAGTACATGGGAAGC	1705	0.10490158016849471	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1700	0.1045939509011384	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.152585347125789E-5	0.0
5	0.0	0.0	0.0	6.152585347125789E-5	0.0
6	0.0	0.0	0.0	6.152585347125789E-5	0.0
7	0.0	0.0	0.0	6.152585347125789E-5	0.0
8	0.0	0.0	0.0	6.152585347125789E-5	0.0
9	0.0	0.0	0.0	6.152585347125789E-5	6.152585347125789E-5
10	0.0	0.0	0.0	6.152585347125789E-5	6.152585347125789E-5
11	0.0	0.0	0.0	6.152585347125789E-5	6.152585347125789E-5
12	0.0	0.0	0.0	6.152585347125789E-5	6.152585347125789E-5
13	0.0	0.0	0.0	6.152585347125789E-5	6.152585347125789E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACG	180	0.0	14.252585	4
GTAGGAC	400	0.0	13.301595	3
ACACCGA	95	7.393646E-8	12.999159	6
GCGTTTA	140	3.6379788E-12	12.890831	13
TGTAGGA	535	0.0	11.8986225	2
GTACCGT	80	2.8684504E-5	11.87423	6
TAAGTAG	105	2.7146416E-7	11.763678	5
TAGGACC	220	0.0	11.661206	4
GTCTACG	75	2.0556798E-4	11.410851	1
TAAGGTG	175	1.8189894E-12	11.401718	5
GTATTAT	155	3.074092E-10	11.042758	1
GTCCTAC	295	0.0	10.959575	1
GTTGGAC	165	8.0035534E-11	10.940705	3
CGTTTAT	165	8.0035534E-11	10.937675	14
GTAGAAC	300	0.0	10.767958	3
CTGTAGG	530	0.0	10.585538	1
GTATTAG	135	5.607035E-8	10.565602	1
CTAGTAC	90	9.503207E-5	10.556821	3
TTAGACT	145	1.4388206E-8	10.484661	4
TACACCG	120	1.5204423E-6	10.293218	5
>>END_MODULE