FastQCFastQC Report
Thu 26 May 2016
SRR1513062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1625333
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95330.5865259611415015No Hit
CCCATGTACTCTGCGTTGATACCAC90560.5571781290357115No Hit
GAGTACATGGGAAGCAGTGGTATCA39170.24099676804691716No Hit
CATGTACTCTGCGTTGATACCACTG26900.16550454583768373No Hit
GTATCAACGCAGAGTACTTTTTTTT26160.16095163268081064No Hit
GTATCAACGCAGAGTACATGGGAAG25110.15449141806632857No Hit
GCGTTGATACCACTGCTTCCCATGT23410.14403202297621473No Hit
ACGCAGAGTACATGGGAAGCAGTGG22600.13904842884504282No Hit
GCTTCCCATGTACTCTGCGTTGATA21280.13092701618683678No Hit
TATCAACGCAGAGTACATGGGAAGC20790.12791224936674514No Hit
ACTCTGCGTTGATACCACTGCTTCC18800.11566860452596484No Hit
TATCAACGCAGAGTACTTTTTTTTT18780.11554555281902232No Hit
GGTATCAACGCAGAGTACTTTTTTT16890.10391716651295459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACT500.001478758913.3247184
TTTGCCG604.1347113E-412.65115819
GATAATC751.4988444E-512.649997
CAATACT1251.369699E-912.1825994
TAGGACG1800.012.1614494
AATGCCG907.5272765E-611.60189318
GTTCTAA1406.493792E-1011.5671511
AGGACGT1950.011.2259535
GTCCTAC3650.011.22228051
ATGCCGG855.3898086E-511.16278619
GTCCTAA1657.6397555E-1110.9692051
GCCGCTC700.00149945210.85019313
CCGTGCG700.00150794610.8428499
GTATAAT2200.010.8248741
GTCTTAT1502.0390871E-910.7960071
ATAAGAC1252.1830601E-710.6597743
CGTGAAA909.642689E-510.5416589
TGTACCG1002.3498966E-510.4694215
GTAGGAC3300.010.3828973
GTACCGT750.002607204510.1521666
Produced by FastQC (version 0.11.5)