##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1625333 Sequences flagged as poor quality 0 Sequence length 25 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.393436914158514 31.0 31.0 34.0 26.0 34.0 2 30.522753798760007 31.0 31.0 34.0 27.0 34.0 3 30.541562867424705 31.0 31.0 34.0 27.0 34.0 4 33.77718965898065 37.0 35.0 37.0 28.0 37.0 5 33.70804259803991 37.0 35.0 37.0 28.0 37.0 6 33.67496691447229 37.0 35.0 37.0 29.0 37.0 7 33.578352251507845 37.0 35.0 37.0 28.0 37.0 8 33.57649355547448 37.0 35.0 37.0 28.0 37.0 9 35.10707959538138 39.0 35.0 39.0 28.0 39.0 10 35.00534413563251 39.0 35.0 39.0 28.0 39.0 11 35.029825887987265 39.0 35.0 39.0 27.0 39.0 12 34.936814179002084 39.0 35.0 39.0 27.0 39.0 13 34.916354371688755 39.0 35.0 39.0 27.0 39.0 14 36.07586814517394 40.0 36.0 41.0 27.0 41.0 15 36.055920848220026 40.0 36.0 41.0 27.0 41.0 16 36.01030065838816 40.0 36.0 41.0 27.0 41.0 17 35.90957114634355 40.0 36.0 41.0 27.0 41.0 18 35.819524368237154 40.0 36.0 41.0 27.0 41.0 19 35.877709367864924 40.0 36.0 41.0 27.0 41.0 20 35.77825713253838 40.0 36.0 41.0 27.0 41.0 21 35.7950549210531 40.0 36.0 41.0 27.0 41.0 22 35.7675147185223 40.0 36.0 41.0 27.0 41.0 23 35.70245358951058 40.0 36.0 41.0 27.0 41.0 24 35.57283522822708 40.0 36.0 41.0 26.0 41.0 25 35.55009650330117 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7239324498600155 1101 2 0.6393394520160385 1101 3 0.6437771621602693 1101 4 0.7787882287545855 1101 5 0.8031786167778421 1101 6 0.6796187155294504 1101 7 0.5114391030578531 1101 8 0.6386611104053728 1101 9 0.7286932449103176 1101 10 0.6570369491769554 1101 11 0.6985700039883511 1101 12 0.5974113835234007 1101 13 0.6466955704044466 1101 14 0.6009817359815486 1101 15 0.6746137423324328 1101 16 0.6575827469264084 1101 17 0.6738378642201255 1101 18 0.6792009951977178 1101 19 0.7093636807430101 1101 20 0.9501123242734977 1101 21 0.9305364259165643 1101 22 0.9419445770130324 1101 23 0.7404360840806561 1101 24 0.8577142254376398 1101 25 0.8345417706584612 1102 1 0.7512711874752007 1102 2 0.6999644087124963 1102 3 0.6718249230785354 1102 4 1.0362075686931504 1102 5 0.7084725034726844 1102 6 0.6604034911593715 1102 7 0.6666477683849976 1102 8 0.6553035270893588 1102 9 0.8339968315982276 1102 10 0.6645137962172996 1102 11 0.6926585510679644 1102 12 0.7050457399750485 1102 13 0.7668461512492684 1102 14 0.7825688396098087 1102 15 0.8655571821347365 1102 16 0.887006983051954 1102 17 0.9284927061333335 1102 18 0.9155311644856923 1102 19 0.9110796557077876 1102 20 0.8908703094463704 1102 21 0.8352159797168852 1102 22 0.9215339439730101 1102 23 0.920339084149937 1102 24 0.9509644159732176 1102 25 0.9297989359509629 1103 1 -0.14606570666118657 1103 2 -0.0022868621662262 1103 3 0.24223933496037375 1103 4 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11 -0.5452925377994831 2214 12 -0.5184981850348649 2214 13 -0.5547015292498685 2214 14 -0.7626852543971196 2214 15 -0.7228138183066477 2214 16 -0.7081360172996511 2214 17 -0.5666086995567525 2214 18 -0.5876814994429083 2214 19 -0.5550640852681923 2214 20 -0.41539297877621095 2214 21 -0.6360371716772093 2214 22 -0.4851807510618187 2214 23 -0.6352861449573481 2214 24 -0.5344629392496856 2214 25 -0.6221507015919627 2215 1 -0.26809340741936083 2215 2 -0.17811087800596326 2215 3 -0.185044296307332 2215 4 0.07893786836019245 2215 5 -0.11604284983845048 2215 6 -0.39231570972852836 2215 7 -0.4503463122661273 2215 8 -0.5659136390223694 2215 9 -0.635899579796515 2215 10 -0.6633506136976095 2215 11 -0.573806487777766 2215 12 -0.6818604753412671 2215 13 -0.6400119693574595 2215 14 -0.7190053372307403 2215 15 -0.7978334948907886 2215 16 -0.9371624583094942 2215 17 -0.8357414781063994 2215 18 -0.9546991351813432 2215 19 -0.767985272150149 2215 20 -0.5494682033172253 2215 21 -0.8540283784037186 2215 22 -0.8591375321646169 2215 23 -0.7999541085988611 2215 24 -0.7534445740378857 2215 25 -0.8505626992691617 2216 1 -1.0351590626212577 2216 2 -0.6204431818729326 2216 3 -2.2754650555247125 2216 4 -0.6474800387290642 2216 5 -0.9098978357184464 2216 6 -0.7159338058345597 2216 7 -0.7848089645765199 2216 8 -0.8205321739173428 2216 9 -0.8139438187227199 2216 10 -0.8230634600877238 2216 11 -0.8850498918798806 2216 12 -0.799789925955011 2216 13 -0.9407251420265368 2216 14 -1.1112503052216525 2216 15 -1.0467182396618924 2216 16 -1.144279277427188 2216 17 -1.0860044701969471 2216 18 -1.188068049797515 2216 19 -1.1540611770117053 2216 20 -1.030525740923693 2216 21 -1.0876687233593358 2216 22 -1.2395323859578937 2216 23 -1.2746316268536049 2216 24 -1.150047194395441 2216 25 -1.0740293288075335 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43796.0 3 0.0 4 720.0 5 5523.0 6 6517.0 7 4822.0 8 3953.0 9 3549.0 10 3158.0 11 2971.0 12 2651.0 13 2469.0 14 2521.0 15 2628.0 16 2481.0 17 2707.0 18 3027.0 19 3650.0 20 4574.0 21 6101.0 22 7661.0 23 9219.0 24 10707.0 25 12248.0 26 13272.0 27 14832.0 28 16693.0 29 19856.0 30 25012.0 31 31516.0 32 40414.0 33 52615.0 34 69553.0 35 93778.0 36 136142.0 37 223895.0 38 740102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.621457748158726 18.37061500426226 13.219290930171063 27.788636317407956 2 14.287974537428932 19.1337431395424 38.342347223034515 28.23593509999415 3 17.934786286871677 22.993811114399325 28.40900910767209 30.66239349105691 4 10.820613375843596 15.919630008127566 35.716803879574215 37.54295273645462 5 13.515421158863782 37.22665288239059 34.986769556441686 14.271156402303944 6 35.0780106195958 34.92314408700683 16.451358138736456 13.547487154660908 7 29.83977249934474 30.288916480138607 20.21031013084241 19.66100088967424 8 26.868378652165298 36.20377903792208 18.593132446035284 18.334709863877336 9 26.20457736011861 15.066133357294916 18.724070252575203 40.00521903001127 10 14.91259373746373 29.181176951228217 32.92802980894416 22.978199502363893 11 36.10921741099336 22.280270625470692 21.50427743786886 20.106234525667087 12 23.428564396340075 24.638714719075544 29.998584763440135 21.93413612114425 13 29.425287073420158 20.209587286194754 24.817344883133952 25.547780757251132 14 21.642364531413527 20.60188161838131 26.400861870063615 31.35489198014155 15 25.215263579832563 28.019796558612914 22.107592720999328 24.657347140555196 16 23.59527746173721 27.449953634849365 25.65220414449682 23.3025647589166 17 23.48530210878907 27.49721377928455 26.10228799337338 22.915196118553 18 23.578811926294858 26.81926479551317 26.821788944228626 22.780134333963346 19 23.966818554432194 26.75747930313795 26.78628135916164 22.489420783268223 20 24.169816277648952 26.25357388301351 26.646662560841378 22.92994727849616 21 23.672072123066474 25.993688677950917 26.88741322547441 23.446825973508197 22 23.623097543703352 26.197216201233843 25.865530325170287 24.314155929892518 23 22.993451168010395 26.213662719155767 26.345205398541093 24.447680714292748 24 23.243327051535964 27.004099472661263 26.295136190720537 23.45743728508224 25 23.57658399847908 25.772503234721743 25.908352319186285 24.742560447612888 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 59.0 1 59.0 2 150.0 3 241.0 4 241.0 5 241.0 6 593.0 7 945.0 8 945.0 9 945.0 10 1338.5 11 1732.0 12 1732.0 13 1732.0 14 3081.5 15 4431.0 16 4431.0 17 4431.0 18 8353.0 19 12275.0 20 12275.0 21 12275.0 22 19682.0 23 27089.0 24 27089.0 25 27089.0 26 38979.0 27 50869.0 28 50869.0 29 50869.0 30 66960.5 31 83052.0 32 83052.0 33 83052.0 34 100171.5 35 117291.0 36 117291.0 37 117291.0 38 134318.5 39 151346.0 40 151346.0 41 151346.0 42 168685.0 43 186024.0 44 186024.0 45 186024.0 46 213614.5 47 241205.0 48 241205.0 49 241205.0 50 236644.0 51 232083.0 52 232083.0 53 232083.0 54 209783.0 55 187483.0 56 187483.0 57 187483.0 58 163601.5 59 139720.0 60 139720.0 61 139720.0 62 116610.0 63 93500.0 64 93500.0 65 93500.0 66 73625.0 67 53750.0 68 53750.0 69 53750.0 70 39755.0 71 25760.0 72 25760.0 73 25760.0 74 18017.0 75 10274.0 76 10274.0 77 10274.0 78 7183.5 79 4093.0 80 4093.0 81 4093.0 82 2813.5 83 1534.0 84 1534.0 85 1534.0 86 990.0 87 446.0 88 446.0 89 446.0 90 281.0 91 116.0 92 116.0 93 116.0 94 65.0 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1817473711540958 2 0.0607875432296028 3 0.0 4 0.0 5 0.01802707506707856 6 0.36140286329016885 7 0.0011689912159538998 8 0.029224780398847496 9 0.03137818527034153 10 0.0030147668200916364 11 0.0037530770617467315 12 0.010028714115815036 13 0.008552093632504847 14 0.0018457756041377368 15 0.0 16 0.011689912159538999 17 0.022887617491307938 18 0.06294094810109682 19 0.027625108208594793 20 0.0 21 0.0 22 0.0 23 3.0762926735628947E-4 24 1.2305170694251578E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1625333.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.49809242788832 #Duplication Level Percentage of deduplicated Percentage of total 1 80.79138440723254 55.3405571650367 2 12.552505030190586 17.19645299519056 3 3.4810110067539988 7.15327841049396 4 1.3397627349107097 3.6708476658941698 5 0.6242962163697456 2.1381549965637903 6 0.35062010152480866 1.4410084872793159 7 0.20872592207992757 1.0008129251908968 8 0.13309469960655024 0.7293386428249209 9 0.10103460279453638 0.6228609804571623 >10 0.38396767663318404 4.505141010271119 >50 0.020188959536016564 0.9581074832627372 >100 0.010079380967362652 1.3247724008872297 >500 0.0017996007568544345 0.8859975113922485 >1k 0.0013497005676408257 1.8869435694799086 >5k 1.7996007568544343E-4 1.1457257557753395 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 9533 0.5865259611415015 No Hit CCCATGTACTCTGCGTTGATACCAC 9056 0.5571781290357115 No Hit GAGTACATGGGAAGCAGTGGTATCA 3917 0.24099676804691716 No Hit CATGTACTCTGCGTTGATACCACTG 2690 0.16550454583768373 No Hit GTATCAACGCAGAGTACTTTTTTTT 2616 0.16095163268081064 No Hit GTATCAACGCAGAGTACATGGGAAG 2511 0.15449141806632857 No Hit GCGTTGATACCACTGCTTCCCATGT 2341 0.14403202297621473 No Hit ACGCAGAGTACATGGGAAGCAGTGG 2260 0.13904842884504282 No Hit GCTTCCCATGTACTCTGCGTTGATA 2128 0.13092701618683678 No Hit TATCAACGCAGAGTACATGGGAAGC 2079 0.12791224936674514 No Hit ACTCTGCGTTGATACCACTGCTTCC 1880 0.11566860452596484 No Hit TATCAACGCAGAGTACTTTTTTTTT 1878 0.11554555281902232 No Hit GGTATCAACGCAGAGTACTTTTTTT 1689 0.10391716651295459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 6.152585347125789E-5 13 0.0 0.0 0.0 0.0 6.152585347125789E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACGACT 50 0.0014787589 13.324718 4 TTTGCCG 60 4.1347113E-4 12.651158 19 GATAATC 75 1.4988444E-5 12.64999 7 CAATACT 125 1.369699E-9 12.182599 4 TAGGACG 180 0.0 12.161449 4 AATGCCG 90 7.5272765E-6 11.601893 18 GTTCTAA 140 6.493792E-10 11.567151 1 AGGACGT 195 0.0 11.225953 5 GTCCTAC 365 0.0 11.2222805 1 ATGCCGG 85 5.3898086E-5 11.162786 19 GTCCTAA 165 7.6397555E-11 10.969205 1 GCCGCTC 70 0.001499452 10.850193 13 CCGTGCG 70 0.001507946 10.842849 9 GTATAAT 220 0.0 10.824874 1 GTCTTAT 150 2.0390871E-9 10.796007 1 ATAAGAC 125 2.1830601E-7 10.659774 3 CGTGAAA 90 9.642689E-5 10.541658 9 TGTACCG 100 2.3498966E-5 10.469421 5 GTAGGAC 330 0.0 10.382897 3 GTACCGT 75 0.0026072045 10.152166 6 >>END_MODULE