FastQCFastQC Report
Thu 26 May 2016
SRR1513061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730950
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC83670.4833761807100147No Hit
CCCATGTACTCTGCGTTGATACCAC66470.3840087813050637No Hit
GAGTACATGGGAAGCAGTGGTATCA37520.21675958288800948No Hit
GTATCAACGCAGAGTACATGGGAAG22580.13044859759091829No Hit
GTATCAACGCAGAGTACTTTTTTTT21500.1242092492561888No Hit
GCGTTGATACCACTGCTTCCCATGT21450.12392039053698836No Hit
CATGTACTCTGCGTTGATACCACTG20860.12051185765042317No Hit
GCTTCCCATGTACTCTGCGTTGATA19570.11305930269505185No Hit
ACGCAGAGTACATGGGAAGCAGTGG18060.10433576937519859No Hit
TATCAACGCAGAGTACATGGGAAGC17390.10046506253791271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGCG350.002171634716.28425416
TAGGACC4400.014.2511934
ACTATAC2000.012.3510333
GTAGGAC7200.012.0079493
GTCCTAC7350.011.7761621
ATTAGGC951.3590699E-511.000923
GTCTAAT1355.586662E-810.5683511
CGTTTAT1803.45608E-1110.55430314
GTATTAG1909.094947E-1210.5127281
TGTAGGA8950.010.50956252
CTTAGAC1909.094947E-1210.5008783
TCCTACA8700.010.4839382
GGGCCGT1002.4004159E-510.4493646
TCGAACT1553.6652636E-910.41872119
TAGGACA3500.010.3151484
TTAGGAC3700.010.271133
CGGGAGT750.002645994310.1341814
TACACCG750.002647263510.1335955
GGACCTG6800.010.0582116
GTCTAGA2650.010.0499041
Produced by FastQC (version 0.11.5)