##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513061_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730950
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.06474017158208	31.0	30.0	34.0	23.0	34.0
2	28.75320430977209	31.0	30.0	34.0	16.0	34.0
3	29.0911193275369	31.0	30.0	34.0	22.0	34.0
4	31.929217481729687	35.0	32.0	37.0	17.0	37.0
5	32.134084751148215	35.0	32.0	37.0	19.0	37.0
6	32.60245298824345	36.0	33.0	37.0	25.0	37.0
7	32.576333227418466	36.0	33.0	37.0	25.0	37.0
8	32.56097403160114	37.0	33.0	37.0	25.0	37.0
9	33.91603050348075	39.0	34.0	39.0	23.0	39.0
10	33.409239434992344	38.0	33.0	39.0	17.0	39.0
11	33.79131459603108	39.0	34.0	39.0	23.0	39.0
12	33.6245449030879	39.0	34.0	39.0	19.0	39.0
13	33.72269216326295	39.0	34.0	39.0	22.0	39.0
14	34.0133158092377	39.0	33.0	41.0	11.0	41.0
15	34.56256448770906	40.0	34.0	41.0	17.0	41.0
16	34.145795661342035	39.0	33.0	41.0	16.0	41.0
17	33.686338138016694	39.0	33.0	41.0	10.0	41.0
18	33.094057598428606	38.0	32.0	41.0	10.0	41.0
19	33.98800947456599	39.0	33.0	41.0	16.0	41.0
20	34.24752708050492	39.0	34.0	41.0	16.0	41.0
21	34.26682457609983	39.0	34.0	41.0	10.0	41.0
22	33.85713452150553	39.0	34.0	41.0	8.0	41.0
23	32.192129755336666	39.0	31.0	41.0	2.0	41.0
24	33.27308067824027	39.0	32.0	41.0	2.0	41.0
25	33.013671105462315	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0817852630008495
1101	2	1.386887770188327
1101	3	1.1162978947723126
1101	4	1.1414675949692104
1101	5	1.4728498101003709
1101	6	1.05291662097126
1101	7	1.092617256550362
1101	8	1.1537590062535727
1101	9	1.3243457365909066
1101	10	1.632101915921119
1101	11	1.2985743662616969
1101	12	1.3323197101806485
1101	13	1.2611017710232773
1101	14	0.5629255601955734
1101	15	1.0294951697131651
1101	16	1.6372474257476881
1101	17	2.310740651298758
1101	18	-4.212933330534163
1101	19	-0.38094954151590343
1101	20	0.6545363721691757
1101	21	1.195474360970131
1101	22	1.7339134528244884
1101	23	3.4400946371924945
1101	24	2.492046163741726
1101	25	2.755206093936131
1102	1	1.1666869851210535
1102	2	1.408366195021756
1102	3	1.2016962244822267
1102	4	1.6965406144606199
1102	5	1.5238115988726975
1102	6	1.1081995407190064
1102	7	1.0833118739573067
1102	8	1.1234589075183692
1102	9	1.2042493700933292
1102	10	1.5822160125409042
1102	11	1.2305356085897827
1102	12	1.2875505652838015
1102	13	1.222345271737403
1102	14	1.9887316827163488
1102	15	1.4399498441566934
1102	16	1.8377602155917998
1102	17	1.4863609124193715
1102	18	1.8903639796937242
1102	19	1.6880273178603034
1102	20	1.5873507007346035
1102	21	1.5759132987575697
1102	22	1.9584721346039728
1102	23	3.55670947374
1102	24	2.4776081921990425
1102	25	2.743135656569663
1103	1	0.9565754080362225
1103	2	1.1587904863988996
1103	3	0.9068681972091461
1103	4	1.3998170439939024
1103	5	1.328462282417064
1103	6	0.9181329924078199
1103	7	0.9494812541543567
1103	8	0.8792690050017526
1103	9	0.9171586647096532
1103	10	1.3792303395549652
1103	11	1.1246895662542045
1103	12	1.2103493724121321
1103	13	1.0455475257816431
1103	14	1.787058559398858
1103	15	1.2879507892635118
1103	16	1.4998028069846967
1103	17	1.9392435474435104
1103	18	2.095413076690349
1103	19	1.634779490244
1103	20	1.452639224792783
1103	21	1.4842198757772636
1103	22	1.776126327360764
1103	23	3.2115328939134073
1103	24	2.0210081962258144
1103	25	2.2653820854752453
1104	1	0.6985872945589655
1104	2	1.1123097078645259
1104	3	0.8566113978056045
1104	4	1.4318975083836385
1104	5	1.177167156560273
1104	6	0.8380869432321916
1104	7	0.7002217832528146
1104	8	0.7261877869647293
1104	9	0.7846006404995336
1104	10	1.2558962777535854
1104	11	0.8438890625156645
1104	12	0.9895119762837012
1104	13	0.7023439308783068
1104	14	1.6463496696475914
1104	15	1.1868173198854066
1104	16	1.5128313331400634
1104	17	1.9669837645884982
1104	18	2.57588801863875
1104	19	1.581090626060238
1104	20	1.0767012741252557
1104	21	1.2045054429415316
1104	22	1.4878253897128175
1104	23	3.103567297595035
1104	24	2.0941921340242544
1104	25	2.11010018275514
1105	1	0.7209798087528405
1105	2	1.1312645510189157
1105	3	0.754217208297348
1105	4	0.9061389798795041
1105	5	1.0279855390898192
1105	6	0.7139428707537618
1105	7	0.5333429480863714
1105	8	0.6737729551899463
1105	9	0.8270262677950768
1105	10	1.293936313778545
1105	11	0.751790078787586
1105	12	0.8476551581102996
1105	13	0.9473087991367706
1105	14	1.6880684168429383
1105	15	1.0806286002786152
1105	16	1.3773531035370823
1105	17	1.807719489727532
1105	18	1.7491370373119537
1105	19	1.293016471175143
1105	20	1.180956686630111
1105	21	1.0132665963897907
1105	22	1.4884024899316657
1105	23	2.785818851748296
1105	24	1.9123652492742025
1105	25	1.9529566906534725
1106	1	-0.571797377116809
1106	2	-0.1194316789961789
1106	3	-0.2377986554551228
1106	4	-2.5904768567676335
1106	5	-0.4513951200763451
1106	6	-0.48929573609038357
1106	7	-0.38589437016269557
1106	8	-0.2719012331822199
1106	9	-0.314412326617294
1106	10	-0.15345495810061038
1106	11	-0.21633728192018964
1106	12	-0.2907302287673659
1106	13	-0.21041060884899565
1106	14	0.4898307583942909
1106	15	-0.02347931329933317
1106	16	-0.9360194767260666
1106	17	-5.685914032493965
1106	18	-6.852066660717178
1106	19	-2.790646568491372
1106	20	-1.4210657030732392
1106	21	-0.9515315540424751
1106	22	-1.2752719101008267
1106	23	-5.07884329163717
1106	24	-2.072791160285334
1106	25	-0.6252361522868455
1107	1	0.5068187915119573
1107	2	0.8818669745957699
1107	3	0.6321269706066808
1107	4	1.0515942230526498
1107	5	0.9852593170446724
1107	6	0.633143619734156
1107	7	0.5711884104965463
1107	8	0.5026884685987341
1107	9	0.6755865742043525
1107	10	1.0273499138223343
1107	11	0.6965153877448174
1107	12	0.7630185958806521
1107	13	0.8050184665255955
1107	14	1.4627081054675486
1107	15	0.9633433607566957
1107	16	-5.821913943074982
1107	17	-0.8823575640571875
1107	18	0.8887309953561839
1107	19	0.7935943463439514
1107	20	0.6819613372836884
1107	21	0.6946426503488823
1107	22	0.9230261289763888
1107	23	2.8021064383237544
1107	24	1.766920587311425
1107	25	1.599808512512169
1108	1	0.24903327979641787
1108	2	0.5173641139501974
1108	3	0.3295020934373625
1108	4	0.8365162404631512
1108	5	0.5801706489477283
1108	6	0.2698917892590629
1108	7	0.218005509303687
1108	8	0.3050776604961527
1108	9	0.3290876574421304
1108	10	0.8606978418148756
1108	11	0.2869219580017557
1108	12	0.3952474552858689
1108	13	0.26136320826385884
1108	14	0.8415201519362014
1108	15	0.5652369900771106
1108	16	0.8145698599015532
1108	17	0.9885399742302425
1108	18	0.4903085764078057
1108	19	0.615490824394044
1108	20	0.4115992936693047
1108	21	0.6392706056980941
1108	22	0.9579485620575667
1108	23	2.604098249057259
1108	24	1.3754559412106602
1108	25	1.5109612615085979
1109	1	0.16645019674554007
1109	2	0.5964615456023132
1109	3	0.4075932511374276
1109	4	0.37685738152518056
1109	5	0.17452085405372486
1109	6	0.17312993005943156
1109	7	0.13753996946726232
1109	8	0.11567562807905318
1109	9	0.1932832507531259
1109	10	0.6893008516528809
1109	11	0.2739006104709958
1109	12	0.28875713297368577
1109	13	0.1881697648765197
1109	14	-0.7822100007465451
1109	15	-0.1767027252533211
1109	16	-0.26595451588961083
1109	17	0.5910566649709637
1109	18	1.457947423404974
1109	19	0.6000366721395451
1109	20	0.41664278887224526
1109	21	0.22964573244686193
1109	22	0.3145563151482804
1109	23	-4.252771510047399
1109	24	-1.4022787698117902
1109	25	-0.15033447707347136
1110	1	0.5234783157159981
1110	2	0.8596816670911664
1110	3	0.6920475543383802
1110	4	1.1964962588703862
1110	5	0.808414416250244
1110	6	0.5502115326795902
1110	7	0.5414979849766937
1110	8	0.4839145415738315
1110	9	0.47543399177232715
1110	10	0.9089814070090156
1110	11	0.4485650226426401
1110	12	0.5186458489273065
1110	13	0.2493246644380136
1110	14	1.12214406321511
1110	15	0.5681286847771219
1110	16	1.1582994564367795
1110	17	1.3331278796219763
1110	18	2.061778606796139
1110	19	1.1965342163439345
1110	20	0.7466603094707267
1110	21	0.6800998228296393
1110	22	1.0634597178371692
1110	23	2.2240744494465687
1110	24	1.4301151085928154
1110	25	1.7304814404378845
1111	1	0.31088351303771944
1111	2	0.6188449797080828
1111	3	0.4413177206385974
1111	4	0.9589179746519427
1111	5	0.6837415149579158
1111	6	0.21405639263293352
1111	7	0.05335508485975282
1111	8	-0.04418981657692456
1111	9	0.07503595499542826
1111	10	0.3654303797434295
1111	11	0.055204414205512364
1111	12	0.2889537928621593
1111	13	0.1489842015492684
1111	14	-0.599003350508724
1111	15	-0.11713206782714991
1111	16	0.3959101467191388
1111	17	0.9718993444889605
1111	18	1.6487885563874443
1111	19	0.680192595336834
1111	20	0.3998322853972667
1111	21	0.4083024616660538
1111	22	0.6540561155844173
1111	23	0.7492886913804142
1111	24	0.6148919616319759
1111	25	0.10931749165274596
1112	1	0.2140019529251873
1112	2	0.5937837382166009
1112	3	0.35780912038272206
1112	4	0.8065689791446395
1112	5	0.5537984753302894
1112	6	-0.0054522869641502325
1112	7	0.009146166247298027
1112	8	-0.0014705290609313693
1112	9	0.03265849583135605
1112	10	0.4517450138978347
1112	11	0.08421089704301465
1112	12	0.1951275302626172
1112	13	-0.07341646491684628
1112	14	0.3257216826327607
1112	15	0.13936151380006834
1112	16	0.4354868613982532
1112	17	0.6414967870574984
1112	18	0.5540095381687209
1112	19	0.10676793146253516
1112	20	0.059517366936887584
1112	21	0.24414220565843436
1112	22	0.1302997063028215
1112	23	1.7704370891839858
1112	24	0.6346082507116506
1112	25	0.660652242108128
1113	1	0.6448572373595276
1113	2	1.015571012575375
1113	3	0.6956339458139631
1113	4	0.7608517782573294
1113	5	0.9325495969886575
1113	6	0.37344914830306664
1113	7	0.3794861027511871
1113	8	0.1610066392128786
1113	9	0.38927798831795
1113	10	0.6436964033877288
1113	11	0.3476468839429572
1113	12	0.39432859065679793
1113	13	0.3882877270108622
1113	14	1.061786472353127
1113	15	0.6481912995804038
1113	16	0.9330686342789463
1113	17	1.3201927937259583
1113	18	1.9399866449506007
1113	19	0.9777715253242789
1113	20	0.8160885768703352
1113	21	0.6873268499845508
1113	22	0.9427666259214789
1113	23	0.758559581110319
1113	24	0.6331561301510291
1113	25	1.105855643750651
1114	1	-0.4860767590789088
1114	2	-2.984327893925677
1114	3	-1.134765713897643
1114	4	-0.06864085522575891
1114	5	-0.006686683855733122
1114	6	-0.003789688799216151
1114	7	-0.2232961694928406
1114	8	-0.18264228328398247
1114	9	0.06827477738367804
1114	10	0.10637178781175294
1114	11	-0.0535363962672335
1114	12	-0.06756683822789
1114	13	-0.1844820608258786
1114	14	0.6373359475115166
1114	15	0.06084050206786884
1114	16	0.3576228119485947
1114	17	0.7848752040201887
1114	18	0.9420563743834478
1114	19	0.3210890147043415
1114	20	0.07714679802401037
1114	21	-0.14460815124648718
1114	22	0.1484805072617661
1114	23	1.796616804637516
1114	24	0.6836413277593891
1114	25	0.6200444362438304
1115	1	0.02784805772763832
1115	2	0.29391693995253476
1115	3	0.08657175688949792
1115	4	0.21306354825170715
1115	5	0.32063268073599716
1115	6	-0.18470794491258857
1115	7	-0.3222360002217428
1115	8	-0.39500471302684304
1115	9	-0.4500872226173058
1115	10	-0.13169746651725944
1115	11	-0.21030932980622907
1115	12	-0.31076828735289297
1115	13	-0.5109325994537244
1115	14	0.4974435354057718
1115	15	-0.16213320844836687
1115	16	0.20620948237837666
1115	17	0.6224931310417574
1115	18	0.9996341643006375
1115	19	0.20262567625901085
1115	20	-0.2557890814397865
1115	21	-0.22343726689769738
1115	22	0.06788878613572535
1115	23	1.787992465414817
1115	24	0.5287236842986189
1115	25	-0.043999567554024566
1116	1	0.12936414843630217
1116	2	0.1195661017949412
1116	3	0.13133108613104838
1116	4	0.8106961996530906
1116	5	0.2953919228358757
1116	6	0.19505404214977773
1116	7	-0.06829240411755677
1116	8	0.08083560858400318
1116	9	0.06381262837044943
1116	10	0.5127512898259283
1116	11	0.1764223639082445
1116	12	0.18258843867661056
1116	13	0.1588992461777181
1116	14	0.7629858034709684
1116	15	0.09283029371709972
1116	16	0.5143763412408262
1116	17	0.7767717578052924
1116	18	1.380045066304028
1116	19	0.5174519012529828
1116	20	0.23318825301283397
1116	21	0.12486553296403713
1116	22	0.560781569934349
1116	23	1.8167853500073008
1116	24	0.9097236639655648
1116	25	1.0180652832739625
1201	1	1.1239407483070174
1201	2	1.4522390171149162
1201	3	1.0919160893631101
1201	4	1.6978035041133417
1201	5	1.4950170036374288
1201	6	1.0231859102816472
1201	7	1.0422279578733935
1201	8	1.0196429776854856
1201	9	1.1216826994758122
1201	10	1.502557366102593
1201	11	1.1665839670110216
1201	12	1.1641204360909612
1201	13	1.1723381602048732
1201	14	2.010076947620931
1201	15	1.5763597054818064
1201	16	1.834589657336494
1201	17	2.2655661986585542
1201	18	2.8063993003053156
1201	19	1.8576296646660424
1201	20	1.7278585826831616
1201	21	1.6371732252717308
1201	22	1.9213019319252993
1201	23	3.5480814068915905
1201	24	2.4155381436035697
1201	25	2.6152857099061535
1202	1	1.18571072590224
1202	2	1.4374481283919884
1202	3	1.2877151931719233
1202	4	1.8127586945539669
1202	5	1.6131499386635682
1202	6	1.1637086884743226
1202	7	1.0964363223244717
1202	8	1.167861797700425
1202	9	1.3139717211337683
1202	10	1.7972026237575633
1202	11	1.4198326600655378
1202	12	1.3708792112963408
1202	13	1.292285882385606
1202	14	2.2546101813774726
1202	15	1.6876218414041375
1202	16	1.8782565729451548
1202	17	2.294360074685443
1202	18	3.0575942218885928
1202	19	2.2060575959580504
1202	20	1.8554351323470897
1202	21	1.9155451446145264
1202	22	2.21211223812395
1202	23	3.7431262164509675
1202	24	2.480620294462419
1202	25	2.638631490339314
1203	1	1.051796226162498
1203	2	1.3384822183581164
1203	3	1.0831476355946563
1203	4	1.680200551510385
1203	5	1.3943487829692032
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2211	18	-5.5696177316645255
2211	19	-2.0642766594998108
2211	20	-1.2341594265308515
2211	21	-0.9729203109561055
2211	22	-0.6004354686052054
2211	23	-5.020590239066806
2211	24	-2.3218253912282023
2211	25	-1.57430245147874
2212	1	-0.06162412896751235
2212	2	0.27923777002008165
2212	3	-0.10749182402048518
2212	4	0.4098286160684417
2212	5	0.3449075994086428
2212	6	-0.07365213744807875
2212	7	-0.3006735216345717
2212	8	-0.31507529225385866
2212	9	-0.5348308201659293
2212	10	-4.222249050767196
2212	11	-1.524840555408339
2212	12	-0.6109722457297977
2212	13	-0.5368829723839852
2212	14	0.29462034085261024
2212	15	-0.17901243895445162
2212	16	0.19878386401411774
2212	17	0.58457220738822
2212	18	1.1371985747228663
2212	19	0.43839577769818305
2212	20	-0.017124411478164347
2212	21	-0.2335427205363274
2212	22	-0.003743983839115117
2212	23	1.3425338188863734
2212	24	0.5488046817816752
2212	25	0.40577717864375273
2213	1	-1.5072762352235891
2213	2	-4.003880788529312
2213	3	-1.7712564578839896
2213	4	-3.6127684046976434
2213	5	-1.40787912337181
2213	6	-1.0035798175637751
2213	7	-0.8533305324794433
2213	8	-0.7394698503605035
2213	9	-0.7789965570003616
2213	10	-0.8252651712572145
2213	11	-0.9335143408507847
2213	12	-0.7258196752116177
2213	13	-1.1888141322327712
2213	14	-6.273092915934534
2213	15	-2.766299126688594
2213	16	-1.9990850227392158
2213	17	-0.7974634066975312
2213	18	-0.09633608769999569
2213	19	-0.6470172180142555
2213	20	-0.7956182104465839
2213	21	-1.0183735415860866
2213	22	-1.0226700729336002
2213	23	-5.557501660426695
2213	24	-3.2147834756512097
2213	25	-2.3932077503610856
2214	1	-0.14972831249675167
2214	2	0.05203697752941139
2214	3	-0.07627542332947357
2214	4	0.248352602199855
2214	5	-0.08255413513114718
2214	6	-0.1984150546835366
2214	7	-0.3771138371811631
2214	8	-0.3513716064474153
2214	9	-0.18967455695271696
2214	10	0.11076011844647127
2214	11	-0.26996299280037306
2214	12	-0.2843356196138487
2214	13	-0.31934065990588323
2214	14	0.30435130476770667
2214	15	-0.07592824890165417
2214	16	0.37668352213171374
2214	17	0.6663400897710048
2214	18	1.129430716501382
2214	19	0.07709372519568092
2214	20	-0.027109069052130508
2214	21	-0.11600403225335043
2214	22	0.3336565018030342
2214	23	1.74194428314577
2214	24	0.7290117441299131
2214	25	0.5129083865879878
2215	1	-2.6723787536568047
2215	2	-4.51655369937318
2215	3	-2.63924016147185
2215	4	-4.395224390187568
2215	5	-5.084999027569989
2215	6	-2.6103684755895173
2215	7	-1.8938977000740778
2215	8	-2.2098826321683624
2215	9	-1.8004880596018609
2215	10	-3.3069670414106973
2215	11	-2.2468539254195754
2215	12	-1.9674762721677794
2215	13	-1.9559486380958226
2215	14	-1.17123306921183
2215	15	-1.7627788843063144
2215	16	-2.661796641324898
2215	17	-7.302953987303454
2215	18	-3.372506035414883
2215	19	-2.414664000335158
2215	20	-2.146002934403434
2215	21	-2.1797263392138007
2215	22	-1.8844277551989705
2215	23	-6.573850754382356
2215	24	-3.8455197824925627
2215	25	-3.6751511404959594
2216	1	-0.8868448380269989
2216	2	-0.366992660758342
2216	3	-0.6670352073995289
2216	4	-3.053792721126154
2216	5	-1.1069513756039875
2216	6	-0.9405691197035573
2216	7	-0.8125936885867979
2216	8	-0.7209690579332957
2216	9	-0.8702627040912603
2216	10	-0.38751740219357345
2216	11	-0.93860516043258
2216	12	-1.7264579797557609
2216	13	-1.1292228875897266
2216	14	-6.805425272062859
2216	15	-3.2582201122704717
2216	16	-1.652397284172892
2216	17	-0.7150663463192473
2216	18	-0.06349166259610683
2216	19	-0.6080780380261359
2216	20	-0.945755867328522
2216	21	-1.1681618041942485
2216	22	-0.53231485459564
2216	23	0.5172307877166418
2216	24	-0.3554360509497698
2216	25	-0.33207689036903076
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	93409.0
3	2.0
4	796.0
5	4452.0
6	5546.0
7	4930.0
8	4504.0
9	4231.0
10	3960.0
11	3906.0
12	3812.0
13	3720.0
14	3741.0
15	3808.0
16	3944.0
17	4270.0
18	4736.0
19	5667.0
20	7075.0
21	8665.0
22	10577.0
23	12899.0
24	15460.0
25	17520.0
26	20360.0
27	23077.0
28	25768.0
29	31297.0
30	37022.0
31	41941.0
32	54063.0
33	70011.0
34	91361.0
35	120935.0
36	169070.0
37	234720.0
38	579695.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3675193111601	17.3748389290779	12.032952161068609	25.224689598693388
2	14.249581826231605	18.802560714669664	41.75298906101349	25.194868398085248
3	20.382620937238677	22.997074054789092	27.946446476238467	28.673858531733764
4	11.013240807948184	15.439249009482156	37.99057794462347	35.55693223794619
5	12.869013233099741	37.64533119735916	35.65146634395888	13.834189225582222
6	34.36338569209719	34.99015384046448	17.196109611330943	13.450350856107393
7	30.01182812412423	30.508239527892012	20.24342707039843	19.236505277585326
8	26.478972206989365	36.46988257442895	19.088921800845167	17.962223417736514
9	26.678043263815894	15.502125743952593	18.956654702554765	38.86317628967675
10	14.568722740136748	29.178362365250237	33.797346990788505	22.455567903824512
11	35.69400368427987	22.53362425430314	22.04720223553042	19.725169825886574
12	23.61233658360419	24.71737089201878	30.46500541711809	21.20528710725894
13	29.549102608104782	20.79937273283018	24.674319141240275	24.977205517824768
14	21.124536282633567	21.165216285869477	27.56324469253083	30.147002738966126
15	25.040073409169445	28.615245577478916	22.276308323134405	24.068372690217235
16	22.9118645536888	27.775508140357974	26.82328127934357	22.489346026609653
17	22.835213528622464	27.63967976774262	27.21916400750306	22.305942696131854
18	22.347707405917895	26.77162351440181	28.868044673110617	22.012624406569685
19	23.866778087537043	26.887104305653803	27.160628672612198	22.085488934196963
20	23.843273038471562	26.622965749059112	27.338460409430294	22.195300803039032
21	23.434979915895827	26.313094743460315	27.252433294249606	22.999492046394252
22	23.319121933120307	26.78580200785875	26.527696626193944	23.367379432827004
23	21.61241612774414	26.607853754408513	28.660661708829853	23.119068409017494
24	22.982566862474485	27.250113855194684	26.9501603219848	22.817158960346028
25	23.181353761656208	26.187696774402337	26.844478296448003	23.78647116749345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	140.0
1	140.0
2	249.5
3	359.0
4	359.0
5	359.0
6	708.5
7	1058.0
8	1058.0
9	1058.0
10	1743.0
11	2428.0
12	2428.0
13	2428.0
14	4122.0
15	5816.0
16	5816.0
17	5816.0
18	10474.0
19	15132.0
20	15132.0
21	15132.0
22	24140.0
23	33148.0
24	33148.0
25	33148.0
26	46750.0
27	60352.0
28	60352.0
29	60352.0
30	79240.0
31	98128.0
32	98128.0
33	98128.0
34	117167.5
35	136207.0
36	136207.0
37	136207.0
38	155963.5
39	175720.0
40	175720.0
41	175720.0
42	193518.0
43	211316.0
44	211316.0
45	211316.0
46	234034.5
47	256753.0
48	256753.0
49	256753.0
50	247982.5
51	239212.0
52	239212.0
53	239212.0
54	212912.5
55	186613.0
56	186613.0
57	186613.0
58	160790.0
59	134967.0
60	134967.0
61	134967.0
62	111051.5
63	87136.0
64	87136.0
65	87136.0
66	67806.5
67	48477.0
68	48477.0
69	48477.0
70	35651.0
71	22825.0
72	22825.0
73	22825.0
74	16210.5
75	9596.0
76	9596.0
77	9596.0
78	6631.5
79	3667.0
80	3667.0
81	3667.0
82	2471.0
83	1275.0
84	1275.0
85	1275.0
86	857.0
87	439.0
88	439.0
89	439.0
90	291.5
91	144.0
92	144.0
93	144.0
94	86.5
95	29.0
96	29.0
97	29.0
98	21.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10965076980848666
2	0.046679569022790954
3	0.03287212224500997
4	0.03131228516132759
5	0.03420087235333198
6	0.018833588491868627
7	0.019006903723388893
8	0.02351309974291574
9	0.028712556688523645
10	0.035414078973973834
11	0.020451197319391087
12	0.01877581674802854
13	0.015482827349143535
14	0.02137554522083249
15	0.021722175683873017
16	0.02166440394003293
17	0.026343915191080043
18	0.01929576244258933
19	0.033160980964210406
20	0.02305092579219504
21	0.027557121811721885
22	0.03749386175221699
23	0.028365926225483114
24	0.03940032929893989
25	0.03131228516132759
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1730950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.17730225210826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.9725236696419	65.49154920958843
2	9.833685961126331	14.982073354260686
3	2.338867611433982	5.345058748916189
4	0.7950317033072543	2.4225348145138073
5	0.3684047582980891	1.4032040311994212
6	0.19071610560218513	0.8716943052481594
7	0.11708576959507827	0.6243494641905488
8	0.07503506802488885	0.457277524515157
9	0.05455315134829633	0.374014070915776
>10	0.22997985362121112	2.9561250218503825
>50	0.013738042058973466	0.719103101239133
>100	0.0074575308439693445	1.1191429425371693
>500	0.0018447000617178788	1.0010597980897975
>1k	9.223500308589393E-4	1.3537171744654686
>5k	1.5372500514315655E-4	0.8790964384699198
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	8367	0.4833761807100147	No Hit
CCCATGTACTCTGCGTTGATACCAC	6647	0.3840087813050637	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3752	0.21675958288800948	No Hit
GTATCAACGCAGAGTACATGGGAAG	2258	0.13044859759091829	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2150	0.1242092492561888	No Hit
GCGTTGATACCACTGCTTCCCATGT	2145	0.12392039053698836	No Hit
CATGTACTCTGCGTTGATACCACTG	2086	0.12051185765042317	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1957	0.11305930269505185	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1806	0.10433576937519859	No Hit
TATCAACGCAGAGTACATGGGAAGC	1739	0.10046506253791271	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTGCG	35	0.0021716347	16.284254	16
TAGGACC	440	0.0	14.251193	4
ACTATAC	200	0.0	12.351033	3
GTAGGAC	720	0.0	12.007949	3
GTCCTAC	735	0.0	11.776162	1
ATTAGGC	95	1.3590699E-5	11.00092	3
GTCTAAT	135	5.586662E-8	10.568351	1
CGTTTAT	180	3.45608E-11	10.554303	14
GTATTAG	190	9.094947E-12	10.512728	1
TGTAGGA	895	0.0	10.5095625	2
CTTAGAC	190	9.094947E-12	10.500878	3
TCCTACA	870	0.0	10.483938	2
GGGCCGT	100	2.4004159E-5	10.449364	6
TCGAACT	155	3.6652636E-9	10.418721	19
TAGGACA	350	0.0	10.315148	4
TTAGGAC	370	0.0	10.27113	3
CGGGAGT	75	0.0026459943	10.134181	4
TACACCG	75	0.0026472635	10.133595	5
GGACCTG	680	0.0	10.058211	6
GTCTAGA	265	0.0	10.049904	1
>>END_MODULE