FastQCFastQC Report
Thu 26 May 2016
SRR1513061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1730950
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC97130.5611369479187729No Hit
CCCATGTACTCTGCGTTGATACCAC85190.4921574857737081No Hit
GAGTACATGGGAAGCAGTGGTATCA39680.22923827955746845No Hit
GTATCAACGCAGAGTACTTTTTTTT33020.19076229815996995No Hit
CATGTACTCTGCGTTGATACCACTG25870.14945550131430718No Hit
GTATCAACGCAGAGTACATGGGAAG24240.14003870706837285No Hit
GCGTTGATACCACTGCTTCCCATGT23380.1350703370981253No Hit
GCTTCCCATGTACTCTGCGTTGATA22850.13200843467460066No Hit
ACGCAGAGTACATGGGAAGCAGTGG22730.1313151737485196No Hit
TATCAACGCAGAGTACTTTTTTTTT22520.13010196712787775No Hit
GGTATCAACGCAGAGTACTTTTTTT21450.12392039053698836No Hit
TATCAACGCAGAGTACATGGGAAGC20000.11554348768017562No Hit
ACTCTGCGTTGATACCACTGCTTCC18510.10693549784800255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT551.9812143E-413.8009488
GAGGCGT604.0281145E-412.6904756
AATCGAA604.135749E-412.6508697
TACACGG853.862089E-612.3172255
AATACCG550.003025825112.1136365
TAGGACC4500.012.0559514
GGAATCG657.8987906E-411.7142855
GTCTTAG2050.011.6189311
AGGCGTG1406.9485395E-1011.5213277
ACACCGT1001.8866886E-611.4214296
GTCCTAA4000.011.194841
GTAGGAC7700.011.1247683
TCTAGAC2100.010.877553
CTTGGAC2450.010.4890663
TAGACTG2001.8189894E-1210.4696435
CTAGACA2100.010.4243194
TAGGACG4850.010.4009574
AAGGCGT1105.9113463E-610.3831176
TGTAGGA8950.010.2170942
CGGAATC750.002606895510.152384
Produced by FastQC (version 0.11.5)