##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513061_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1730950
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.354434270198446	31.0	31.0	34.0	26.0	34.0
2	30.502216701811143	31.0	31.0	34.0	27.0	34.0
3	30.521891446893324	31.0	31.0	34.0	26.0	34.0
4	33.75798376613998	37.0	35.0	37.0	28.0	37.0
5	33.64497299170976	37.0	35.0	37.0	28.0	37.0
6	33.591501776481124	37.0	35.0	37.0	28.0	37.0
7	33.505266472168465	37.0	35.0	37.0	28.0	37.0
8	33.49738929489587	37.0	35.0	37.0	28.0	37.0
9	35.035327999075655	39.0	35.0	39.0	28.0	39.0
10	34.939365666252634	39.0	35.0	39.0	27.0	39.0
11	34.94834455068026	39.0	35.0	39.0	27.0	39.0
12	34.85687917039776	39.0	35.0	39.0	27.0	39.0
13	34.82455010254485	39.0	35.0	39.0	27.0	39.0
14	35.999543603223664	40.0	36.0	41.0	27.0	41.0
15	35.979332158641206	40.0	36.0	41.0	27.0	41.0
16	35.93212513359716	40.0	36.0	41.0	27.0	41.0
17	35.84300297524481	40.0	36.0	41.0	27.0	41.0
18	35.763258326352585	40.0	36.0	41.0	27.0	41.0
19	35.83220601403853	40.0	36.0	41.0	27.0	41.0
20	35.73338166902568	40.0	36.0	41.0	26.0	41.0
21	35.75140587538635	40.0	36.0	41.0	27.0	41.0
22	35.730565296513475	40.0	36.0	41.0	27.0	41.0
23	35.6658176146047	40.0	36.0	41.0	27.0	41.0
24	35.53371501198764	40.0	36.0	41.0	25.0	41.0
25	35.51362604350212	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7445334565545281
1101	2	0.6615738298912106
1101	3	0.7683703939275475
1101	4	1.0159317878642113
1101	5	0.795572887680521
1101	6	0.7980925164761601
1101	7	0.7094245600697633
1101	8	0.69008220156406
1101	9	0.730269490020838
1101	10	0.7492137995037282
1101	11	0.6848883095672562
1101	12	0.7437442842850501
1101	13	0.7557298200740448
1101	14	0.7482266812336178
1101	15	0.8340868745293051
1101	16	0.9097518581495478
1101	17	0.8176155418760729
1101	18	0.7716128398286273
1101	19	0.831756847854237
1101	20	0.9545179639612016
1101	21	0.8808083714292678
1101	22	0.8943581196979267
1101	23	0.9421772971229743
1101	24	0.8918833655638636
1101	25	0.9789177381456682
1102	1	0.6188360365687409
1102	2	0.5732920100677141
1102	3	0.6275191135359819
1102	4	0.8375939633689669
1102	5	0.707995230448816
1102	6	0.5196498591154679
1102	7	0.5235244632107623
1102	8	0.5860250289556888
1102	9	0.7118680387774958
1102	10	0.5171268515472676
1102	11	0.682118564285318
1102	12	0.5287255105246942
1102	13	0.6114867157078834
1102	14	0.7136330082605653
1102	15	0.6169737887141054
1102	16	0.674993665590101
1102	17	0.7716503173062534
1102	18	0.5423706852904431
1102	19	0.6249247704045615
1102	20	0.7741239377928508
1102	21	0.8226577920341995
1102	22	0.6839753272974889
1102	23	0.7610943525155349
1102	24	0.8722670726134609
1102	25	0.8055928946327597
1103	1	0.1280836936394536
1103	2	0.13802620173490254
1103	3	0.401886703241189
1103	4	-0.4124537064926628
1103	5	-0.024987517206476184
1103	6	0.06815201876745647
1103	7	0.09874632052331833
1103	8	0.18569323487528067
1103	9	0.28465044273605145
1103	10	0.2620051542396382
1103	11	0.30437636289703107
1103	12	0.351775280228928
1103	13	0.3214745288041243
1103	14	0.3941368638937206
1103	15	0.23548165690605316
1103	16	0.34610803891203545
1103	17	0.30478323982999456
1103	18	0.3375365468003153
1103	19	0.31328142150499616
1103	20	0.5104139671527506
1103	21	0.46577988965023565
1103	22	0.535763805758414
1103	23	0.47145316149144634
1103	24	0.5265631634479391
1103	25	0.6315078894986286
1104	1	0.33591332206885127
1104	2	0.3870383010318257
1104	3	0.4963183626548897
1104	4	0.12556079328908254
1104	5	0.1858973523583387
1104	6	0.24196689831363472
1104	7	0.27514418632335946
1104	8	0.2417869085627231
1104	9	0.23591074051611116
1104	10	0.20243155599764862
1104	11	0.2922733179198005
1104	12	0.3373204751364156
1104	13	0.31798710814035047
1104	14	0.2794602316303525
1104	15	0.33996848191745244
1104	16	0.448860379572146
1104	17	0.310586958246148
1104	18	0.1593693109098666
1104	19	0.30242417221957396
1104	20	0.45003453716842756
1104	21	0.4160723244038067
1104	22	0.47734576158556763
1104	23	0.48132150098216187
1104	24	0.5862887020578
1104	25	0.4646424798333726
1105	1	0.39300421841618416
1105	2	0.3551137323469291
1105	3	0.34618383889861803
1105	4	0.38740473889379246
1105	5	0.4095915687343066
1105	6	0.3423170903052295
1105	7	0.21677101916145602
1105	8	0.22784404761011245
1105	9	0.22554402046541355
1105	10	0.2731348090991901
1105	11	0.24612966085095422
1105	12	0.26630580730310527
1105	13	0.27077059455506003
1105	14	0.27926335369837574
1105	15	0.3277743015835384
1105	16	0.2601857897321267
1105	17	0.22992598403943987
1105	18	0.1963014455766512
1105	19	0.3044315578487371
1105	20	0.41841981616953206
1105	21	0.36455783165224886
1105	22	0.3526728823896974
1105	23	0.3352229748314386
1105	24	0.34984030017381684
1105	25	0.33155442240314414
1106	1	-0.49839845633569624
1106	2	-0.32138674505338116
1106	3	-0.1438007989868062
1106	4	-1.3884896242597264
1106	5	-0.6169616126703872
1106	6	-0.5461713972798137
1106	7	-0.33871812363895515
1106	8	-0.2669597204095808
1106	9	-0.2053456065334771
1106	10	-0.2038867253741543
1106	11	-0.17507497756725598
1106	12	-0.21840100601961865
1106	13	-0.26113064945159437
1106	14	-0.20640510208521334
1106	15	-0.14534592832558246
1106	16	-0.09892259913535639
1106	17	-0.24682711738851282
1106	18	-0.1856042456260667
1106	19	-0.36713144014078125
1106	20	-0.08822775933708726
1106	21	-0.20830885713151304
1106	22	-0.07253437805455576
1106	23	-0.08088871427766975
1106	24	-0.014073826289880742
1106	25	-0.11401866686379947
1107	1	0.31794393373365537
1107	2	0.07589745861468344
1107	3	0.2695961917704146
1107	4	0.58425644788624
1107	5	0.3374388595776381
1107	6	0.22739202502621936
1107	7	0.20169547496275442
1107	8	0.13668875438079198
1107	9	0.16880601857039323
1107	10	0.035987734789742376
1107	11	0.09513367268159811
1107	12	0.13764379147813344
1107	13	0.19848992329436754
1107	14	0.11695266566010787
1107	15	0.24279829412533616
1107	16	0.19424834246182598
1107	17	0.07380107158131466
1107	18	-0.018422350972912227
1107	19	0.05719226617471662
1107	20	0.26789306940921165
1107	21	0.21027263896981907
1107	22	0.24476950830976563
1107	23	0.10940710707907897
1107	24	0.3041596631115908
1107	25	0.29128868447517675
1108	1	-0.5453250777959404
1108	2	-0.3218801165621272
1108	3	-0.12537907028223572
1108	4	-1.004010111186382
1108	5	-0.6715145481736755
1108	6	-0.4964994228544981
1108	7	-0.5234220628480344
1108	8	-0.3633957653228279
1108	9	-0.14544364321223924
1108	10	-0.1419721036640027
1108	11	-0.03553919283564255
1108	12	0.011997769972992955
1108	13	-0.014398860414452486
1108	14	-0.0626918860335266
1108	15	-0.1143214240166941
1108	16	-0.09720787937995112
1108	17	-0.07753478311794737
1108	18	-0.2983707302175347
1108	19	-0.1016796978359622
1108	20	-0.057265730643770496
1108	21	-0.03371953422850993
1108	22	-0.07376073006904704
1108	23	-0.11898411439968015
1108	24	-0.11867744996136764
1108	25	-0.08396203421941806
1109	1	-0.3053773108960023
1109	2	-0.3625743422472034
1109	3	0.095466164012322
1109	4	-0.7242991596932526
1109	5	-0.3293488862423146
1109	6	-0.1248834031054642
1109	7	0.007807634786139772
1109	8	0.08943412697981756
1109	9	0.13504002967253115
1109	10	0.1956295692061616
1109	11	0.16978891957603537
1109	12	0.17377418590433535
1109	13	0.26116656757854173
1109	14	0.2747390661154796
1109	15	0.34161850654034254
1109	16	0.15575930600304844
1109	17	0.14435792407589787
1109	18	0.10291707446693721
1109	19	0.1293296887723372
1109	20	0.31098878570470134
1109	21	0.37325565422222695
1109	22	0.2828087176459704
1109	23	0.3846783893361305
1109	24	0.36223786112444145
1109	25	0.3019246670222131
1110	1	0.3226620070606607
1110	2	0.016192093293913246
1110	3	0.11910414446176532
1110	4	0.6651170408689637
1110	5	0.4965462550489548
1110	6	0.25308073743450876
1110	7	0.16640991049141007
1110	8	0.20630941405288183
1110	9	0.11800867146451566
1110	10	0.28575591959853597
1110	11	0.1468250869917469
1110	12	0.16132955586645892
1110	13	0.1881885851351015
1110	14	0.24858591297804367
1110	15	0.14464803902068013
1110	16	0.2109917950994742
1110	17	0.20858352422381898
1110	18	0.2358799932480835
1110	19	0.08740987560146607
1110	20	0.18348251957812067
1110	21	0.19058251570027096
1110	22	0.33408541871198594
1110	23	0.3429153048090683
1110	24	0.31106797170267697
1110	25	0.42109345236583096
1111	1	0.09102824339516147
1111	2	-0.41135558445965614
1111	3	-0.06731811319167846
1111	4	0.42042509437349906
1111	5	0.136713438463957
1111	6	-0.015806320128092466
1111	7	-0.13107085006828356
1111	8	-0.10186056637731156
1111	9	-0.05706588572454763
1111	10	-0.05050905575492237
1111	11	-0.04951116842116221
1111	12	-0.12215842566739354
1111	13	-0.020873292517400444
1111	14	0.006032368265977084
1111	15	0.01134848413921219
1111	16	-0.056310154377612776
1111	17	-0.0033984850982378134
1111	18	-0.018080353151489703
1111	19	-0.16633319079576836
1111	20	0.03133936977511809
1111	21	0.02583353984478265
1111	22	0.04327778389644976
1111	23	0.11381545628085377
1111	24	0.12220622805721604
1111	25	0.1852567284595139
1112	1	0.15654225868818017
1112	2	-0.30512366350994213
1112	3	0.12955981044902387
1112	4	0.4936239664060551
1112	5	0.12225879353237445
1112	6	0.2156861870870017
1112	7	0.008992160070860677
1112	8	0.07727864768195047
1112	9	-0.026336376416828955
1112	10	0.02865759713722582
1112	11	0.024095649632926097
1112	12	0.06677612114556553
1112	13	0.14437526033210446
1112	14	0.15054708110040593
1112	15	-0.0074738940472443005
1112	16	-0.15282476142377277
1112	17	-0.07670380657887677
1112	18	-0.1161196336590038
1112	19	-0.17889819272971152
1112	20	-0.04120427615153943
1112	21	0.004467614908172379
1112	22	-0.030867231988324306
1112	23	-0.20040281798184623
1112	24	-0.1501648507517359
1112	25	-0.12332765917516042
1113	1	0.2899059584071395
1113	2	0.06406933460885966
1113	3	0.22110899716130206
1113	4	0.6049444031354128
1113	5	0.297148221930712
1113	6	0.4425285536608996
1113	7	0.1999123071049027
1113	8	0.028906817363242965
1113	9	0.004449672658282111
1113	10	0.11150863635086239
1113	11	0.06481059581609827
1113	12	0.17430045600784894
1113	13	0.11718762795364768
1113	14	0.23827010322383302
1113	15	0.3175505888511694
1113	16	0.28683525652275677
1113	17	0.2680381778340575
1113	18	0.10755885919654418
1113	19	-0.036222337194750764
1113	20	0.14663671938004796
1113	21	0.1692978744041227
1113	22	0.07270704343175538
1113	23	0.07713805137740337
1113	24	0.06661470295349403
1113	25	0.058539964639983566
1114	1	0.26401616926002447
1114	2	-0.110285087425531
1114	3	0.12345114158219417
1114	4	0.4636731485471728
1114	5	0.1450649536469939
1114	6	0.10332492985565978
1114	7	-0.033309883097267345
1114	8	-0.046578034735688334
1114	9	0.017584021567522257
1114	10	-0.1239683227771664
1114	11	-0.07494668765058066
1114	12	-0.09619695471891276
1114	13	-0.026145422147266117
1114	14	-0.08040782926823198
1114	15	-0.18001446545523692
1114	16	-0.2230768970656527
1114	17	-0.1776288012413758
1114	18	-0.271008909534288
1114	19	-0.40183759734799196
1114	20	-0.12781075985995471
1114	21	-0.16114977272786746
1114	22	-0.07074360784071132
1114	23	-0.19277109755865496
1114	24	-0.1180517678391837
1114	25	-0.1993468638752418
1115	1	0.013267071152512244
1115	2	-0.15875114494701847
1115	3	-0.07598203183536967
1115	4	0.4845383703829782
1115	5	-0.009579921730320962
1115	6	-0.020091011601110154
1115	7	-0.07671750014907275
1115	8	-0.15916499723260102
1115	9	-0.3913808905492786
1115	10	-0.36843121672975343
1115	11	-0.2958689215806203
1115	12	-0.3499515416233905
1115	13	-0.4287067378064435
1115	14	-0.37426325800458926
1115	15	-0.44707912685650086
1115	16	-0.42806192739588766
1115	17	-0.3699026422044298
1115	18	-0.4078305602642871
1115	19	-0.5984944120756523
1115	20	-0.39873486223292787
1115	21	-0.37610368364936164
1115	22	-0.42559804384384137
1115	23	-0.3543552716503342
1115	24	-0.3865707416313242
1115	25	-0.40749890497705366
1116	1	0.07394253525450267
1116	2	-0.07863286465875419
1116	3	0.08627505350827391
1116	4	0.398629816847496
1116	5	0.12133974236760281
1116	6	0.07213124612326993
1116	7	-0.022434283924454235
1116	8	-0.1786473176408876
1116	9	-0.2248854669391136
1116	10	-0.28944473240218116
1116	11	-0.2844077010744428
1116	12	-0.4198955095320187
1116	13	-0.3713613634313191
1116	14	-0.26332378370121035
1116	15	-0.22271908072951163
1116	16	-0.3100590981964615
1116	17	-0.28962158128369
1116	18	-0.3628295581351253
1116	19	-0.5340433900491419
1116	20	-0.5040103606106356
1116	21	-0.4900031860704459
1116	22	-0.4172559140434231
1116	23	-0.43089319134638515
1116	24	-0.4105158772382822
1116	25	-0.4815959285082059
1201	1	0.5607751144400233
1201	2	0.4808403092261848
1201	3	0.6553151310777636
1201	4	0.9658954923484657
1201	5	0.6508872536798194
1201	6	0.7071377102473875
1201	7	0.6711468408331385
1201	8	0.5343164832640923
1201	9	0.5391449144442078
1201	10	0.5110174524717692
1201	11	0.5176608186411329
1201	12	0.5419499979017246
1201	13	0.5246632002402976
1201	14	0.4663691704035102
1201	15	0.5033474997488341
1201	16	0.545567921842327
1201	17	0.4999097697182435
1201	18	0.6134018575080518
1201	19	0.6149514386515662
1201	20	0.6810025676375844
1201	21	0.5486587402385368
1201	22	0.5998352301065424
1201	23	0.6146465630168976
1201	24	0.6789282080881591
1201	25	0.5231648988996795
1202	1	0.6913725752316573
1202	2	0.5857058805480655
1202	3	0.6674547875378067
1202	4	0.8875683825650427
1202	5	0.6671307189524143
1202	6	0.5102482404938371
1202	7	0.5883611798921393
1202	8	0.6638198439810452
1202	9	0.5255265425248652
1202	10	0.522522306840699
1202	11	0.5301178761467327
1202	12	0.6333801696717032
1202	13	0.4600851569243929
1202	14	0.7175752943766227
1202	15	0.7640607185688495
1202	16	0.7263184819618189
1202	17	0.6397118608310208
1202	18	0.804865636447559
1202	19	0.7076960241631411
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2210	12	-0.0018282826412630016
2210	13	-0.009827749985944934
2210	14	-0.0038174811801994224
2210	15	0.07826831422847391
2210	16	0.01039258247580932
2210	17	0.08477967922051022
2210	18	0.14314733262117585
2210	19	0.2565973662533807
2210	20	0.21030686628012063
2210	21	0.14459765426568794
2210	22	0.1362945061246421
2210	23	0.1152517666368098
2210	24	0.21891689587096153
2210	25	0.20239566813044974
2211	1	0.14357422095963557
2211	2	0.16861568267306382
2211	3	0.17487606923216958
2211	4	0.4575442428719896
2211	5	0.24488837053030466
2211	6	0.10653530901884523
2211	7	0.051935616485799585
2211	8	-0.17280553265102583
2211	9	-0.12059471978726322
2211	10	-0.13764860923422617
2211	11	-0.1832019483559364
2211	12	-0.19695479059471666
2211	13	-0.15809960863865768
2211	14	-0.1766456278653763
2211	15	-0.28666576285063883
2211	16	-0.3168940354381462
2211	17	-0.12104862760131141
2211	18	-0.16522581246263002
2211	19	-0.15429259317867405
2211	20	-0.0752748301007955
2211	21	-0.1534953851279255
2211	22	-0.20282287403976085
2211	23	-0.2963027598924981
2211	24	-3.2028043062214877
2211	25	-2.3472867275138327
2212	1	-0.09073387031140712
2212	2	-0.20601729521801104
2212	3	-0.4850621151100931
2212	4	0.06667549348218671
2212	5	-0.19571951464396875
2212	6	-0.192097310443053
2212	7	-0.21168689875078428
2212	8	-0.2293933404420443
2212	9	-0.41888495914572843
2212	10	-0.3191262468650038
2212	11	-0.4352023977298174
2212	12	-0.45162888084664843
2212	13	-0.34995898671055414
2212	14	-0.42422121562903214
2212	15	-0.38104992670091065
2212	16	-0.58033225388467
2212	17	-0.5328817386996434
2212	18	-0.6101381912420152
2212	19	-0.4981240672721299
2212	20	-0.7481343860498484
2212	21	-0.6636087049176354
2212	22	-0.5809640443110027
2212	23	-0.6589666108394567
2212	24	-0.5638386418636614
2212	25	-0.4702830348909188
2213	1	-0.15666125381896734
2213	2	-0.5107652446030215
2213	3	-0.48125383979711955
2213	4	0.12194622824495838
2213	5	-0.11426458676764639
2213	6	-0.20928069140237682
2213	7	-0.2894658883400041
2213	8	-0.4220691490984052
2213	9	-0.36407537426451597
2213	10	-0.38181949207469046
2213	11	-0.6242482748360842
2213	12	-0.569445098004941
2213	13	-0.47528566837180364
2213	14	-0.5612953116440096
2213	15	-0.5362029112797231
2213	16	-0.6715341149383747
2213	17	-0.6452362796051787
2213	18	-0.5203824301836875
2213	19	-0.5841455697384959
2213	20	-6.232548521376231
2213	21	-3.2016248835926433
2213	22	-1.8970070535737875
2213	23	-1.3354958138864035
2213	24	-1.0246750010420413
2213	25	-0.8578962892694761
2214	1	-0.15016463006351444
2214	2	-0.31218809145367743
2214	3	-0.057920517806493876
2214	4	0.2927467451583752
2214	5	-0.06774918472140712
2214	6	-0.21837876543659007
2214	7	-0.3490861763278872
2214	8	-0.3595261067901845
2214	9	-0.46229677643796663
2214	10	-0.5085085632089204
2214	11	-0.6785308585341525
2214	12	-0.46587800801965784
2214	13	-0.46920104670306273
2214	14	-0.4127083557766582
2214	15	-0.5111404107725761
2214	16	-0.5950042038224552
2214	17	-0.47267781732051617
2214	18	-0.4527273162405052
2214	19	-0.5006489232103775
2214	20	-0.4329562568593772
2214	21	-0.5055213134924799
2214	22	-0.624871168270559
2214	23	-0.6642881373504466
2214	24	-0.45319800497176743
2214	25	-0.5664389719265728
2215	1	-0.3408918639445666
2215	2	-0.3765136063962302
2215	3	-0.33103336065280686
2215	4	-0.028656034624042093
2215	5	-0.35201252455514265
2215	6	-0.5225904271934994
2215	7	-0.6084184462590656
2215	8	-0.5964296358477768
2215	9	-0.8167595128409104
2215	10	-0.9320999494038844
2215	11	-0.9230402794965755
2215	12	-0.9135654054167404
2215	13	-0.9999011516981398
2215	14	-0.9370036620436579
2215	15	-0.9394019859165965
2215	16	-0.9628547409412249
2215	17	-0.992737917780083
2215	18	-0.9516605306074766
2215	19	-0.9355456623717586
2215	20	-0.8509806263133086
2215	21	-0.9441451179387101
2215	22	-0.8890781212173664
2215	23	-0.8397465221889675
2215	24	-0.9523019198943175
2215	25	-0.8602215600737892
2216	1	-0.8385324999840265
2216	2	-0.4804890347339388
2216	3	-2.2128487620658817
2216	4	-0.6327638705633447
2216	5	-0.7566884881618634
2216	6	-0.5400103205945399
2216	7	-0.48075559692485115
2216	8	-0.558954240274943
2216	9	-0.5282944012181972
2216	10	-0.47541352964096006
2216	11	-0.5833708800010982
2216	12	-0.6870350484390428
2216	13	-0.711749738828729
2216	14	-0.7679020983210165
2216	15	-0.8031413758709931
2216	16	-0.6270688288521384
2216	17	-0.8043563067634878
2216	18	-0.9527403060995141
2216	19	-0.7629045544946749
2216	20	-0.8124784417344131
2216	21	-0.8556998879709141
2216	22	-0.8991735707449848
2216	23	-0.8055172522430993
2216	24	-0.7714170760573325
2216	25	-0.8067757797264505
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51839.0
3	4.0
4	925.0
5	6867.0
6	7819.0
7	5948.0
8	4833.0
9	4110.0
10	3584.0
11	3371.0
12	3070.0
13	2850.0
14	2741.0
15	2779.0
16	2715.0
17	2897.0
18	3163.0
19	3842.0
20	4750.0
21	6262.0
22	7852.0
23	9493.0
24	10889.0
25	12175.0
26	13263.0
27	14480.0
28	16298.0
29	19371.0
30	24457.0
31	31300.0
32	39551.0
33	51559.0
34	69124.0
35	94626.0
36	138227.0
37	231725.0
38	822191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94268747250712	18.349038038571063	13.129210751742178	27.579063737179638
2	13.871197305111075	19.106737048883886	39.35956498841191	27.662500657593135
3	17.933630626898886	22.94180822196379	28.54497735057474	30.57958380056258
4	10.560385915248851	15.968687714838673	36.29030301279644	37.18062335711603
5	13.15286153106531	37.497081957134235	35.290405937321594	14.059650574478855
6	34.49683335478835	35.444797389213804	16.722565695855934	13.335803560141905
7	29.623420132981227	30.354707118349133	20.303006318601486	19.718866430068154
8	26.330808861560513	36.97392791819209	18.672788582953604	18.022474637293797
9	26.11985237771056	15.188035798289606	18.847212317422596	39.844899506577235
10	14.5931861643432	29.519553524487403	33.31904396004368	22.568216351125724
11	35.648654207464894	22.39293860449853	21.93623344479905	20.022173743237527
12	23.309910178499	24.834321912956458	30.365745571613957	21.490022336930583
13	29.411982218606674	20.543240747909348	24.928038973929944	25.116738059554034
14	21.17220899868278	21.112067571002704	26.785640006470544	30.930083423843968
15	24.78802090645074	28.774735708562183	22.252244289115392	24.18499909587169
16	22.93265339021097	27.944518305249854	26.359987357957582	22.76284094658159
17	22.98209938289476	27.954422394927708	26.545060124276294	22.51841809790124
18	22.816339107175168	27.144726048422573	27.696866655528662	22.342068188873597
19	23.653999514579937	26.939773239485454	27.143823029714643	22.262404216219963
20	23.777867644934865	26.551720153672836	27.279875213033307	22.390536988358996
21	23.179179063520035	26.266385510846646	27.26988070134897	23.284554724284355
22	23.217886131892893	26.754614518039226	26.346803778272047	23.680695571795834
23	22.394613802996403	26.769339025028554	26.896437375465283	23.939609796509764
24	22.781050560301523	27.258667674209953	27.05444373384912	22.9058380316394
25	23.006801471331624	26.20533591688721	26.671900789682425	24.115961822098743
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	84.0
1	84.0
2	202.5
3	321.0
4	321.0
5	321.0
6	706.5
7	1092.0
8	1092.0
9	1092.0
10	1686.0
11	2280.0
12	2280.0
13	2280.0
14	3837.0
15	5394.0
16	5394.0
17	5394.0
18	10030.0
19	14666.0
20	14666.0
21	14666.0
22	23435.0
23	32204.0
24	32204.0
25	32204.0
26	45774.5
27	59345.0
28	59345.0
29	59345.0
30	77705.0
31	96065.0
32	96065.0
33	96065.0
34	114797.5
35	133530.0
36	133530.0
37	133530.0
38	152060.5
39	170591.0
40	170591.0
41	170591.0
42	186957.0
43	203323.0
44	203323.0
45	203323.0
46	231046.5
47	258770.0
48	258770.0
49	258770.0
50	251632.0
51	244494.0
52	244494.0
53	244494.0
54	217387.0
55	190280.0
56	190280.0
57	190280.0
58	164665.0
59	139050.0
60	139050.0
61	139050.0
62	114941.0
63	90832.0
64	90832.0
65	90832.0
66	70524.0
67	50216.0
68	50216.0
69	50216.0
70	36788.5
71	23361.0
72	23361.0
73	23361.0
74	16477.0
75	9593.0
76	9593.0
77	9593.0
78	6589.5
79	3586.0
80	3586.0
81	3586.0
82	2454.5
83	1323.0
84	1323.0
85	1323.0
86	874.5
87	426.0
88	426.0
89	426.0
90	269.5
91	113.0
92	113.0
93	113.0
94	62.0
95	11.0
96	11.0
97	11.0
98	5.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18660273260348365
2	0.0668419076229816
3	5.777174384008782E-5
4	0.0
5	0.01952684941794968
6	0.37043242150264305
7	0.0016753805713625467
8	0.03206331783124874
9	0.033969785377971635
10	0.003466304630405269
11	0.004275109044166498
12	0.010630000866576157
13	0.008781305063693347
14	0.0017331523152026346
15	5.777174384008782E-5
16	0.012536468413299057
17	0.026055056471879605
18	0.06568647274617985
19	0.028308154481643026
20	0.0
21	0.0
22	0.0
23	4.0440220688061474E-4
24	4.0440220688061474E-4
25	5.777174384008782E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1730950.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.76467868695148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.89596635977935	59.49002390253904
2	11.749879192928688	16.86452609782048
3	2.9369231026251574	6.323020283645373
4	1.04504165924432	2.9998831556058905
5	0.462627236137152	1.6600147476607572
6	0.25167184917157853	1.083668963420955
7	0.16021030207715392	0.8048208595634472
8	0.1051160831175223	0.6034897543807899
9	0.06976813321881468	0.45061988967329963
>10	0.29451724967098925	3.600670969525779
>50	0.01546059085429619	0.7607964287216549
>100	0.00935229168454331	1.2623571331689105
>500	0.001853879960031522	0.9574475589145539
>1k	0.0014508625774159736	2.0840333888698375
>5k	1.6120695304621928E-4	1.0546268664892549
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	9713	0.5611369479187729	No Hit
CCCATGTACTCTGCGTTGATACCAC	8519	0.4921574857737081	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3968	0.22923827955746845	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3302	0.19076229815996995	No Hit
CATGTACTCTGCGTTGATACCACTG	2587	0.14945550131430718	No Hit
GTATCAACGCAGAGTACATGGGAAG	2424	0.14003870706837285	No Hit
GCGTTGATACCACTGCTTCCCATGT	2338	0.1350703370981253	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2285	0.13200843467460066	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2273	0.1313151737485196	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2252	0.13010196712787775	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2145	0.12392039053698836	No Hit
TATCAACGCAGAGTACATGGGAAGC	2000	0.11554348768017562	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1851	0.10693549784800255	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
7	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
8	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
9	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
10	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
11	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
12	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
13	5.777174384008781E-5	0.0	0.0	5.777174384008781E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	55	1.9812143E-4	13.800948	8
GAGGCGT	60	4.0281145E-4	12.690475	6
AATCGAA	60	4.135749E-4	12.650869	7
TACACGG	85	3.862089E-6	12.317225	5
AATACCG	55	0.0030258251	12.113636	5
TAGGACC	450	0.0	12.055951	4
GGAATCG	65	7.8987906E-4	11.714285	5
GTCTTAG	205	0.0	11.618931	1
AGGCGTG	140	6.9485395E-10	11.521327	7
ACACCGT	100	1.8866886E-6	11.421429	6
GTCCTAA	400	0.0	11.19484	1
GTAGGAC	770	0.0	11.124768	3
TCTAGAC	210	0.0	10.87755	3
CTTGGAC	245	0.0	10.489066	3
TAGACTG	200	1.8189894E-12	10.469643	5
CTAGACA	210	0.0	10.424319	4
TAGGACG	485	0.0	10.400957	4
AAGGCGT	110	5.9113463E-6	10.383117	6
TGTAGGA	895	0.0	10.217094	2
CGGAATC	75	0.0026068955	10.15238	4
>>END_MODULE