FastQCFastQC Report
Thu 26 May 2016
SRR1513060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585378
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC74300.46865794782064596No Hit
CCCATGTACTCTGCGTTGATACCAC58180.3669787268399082No Hit
GAGTACATGGGAAGCAGTGGTATCA34160.2154691184058313No Hit
GCGTTGATACCACTGCTTCCCATGT20690.13050515397589724No Hit
CATGTACTCTGCGTTGATACCACTG20310.12810824926295178No Hit
GTATCAACGCAGAGTACATGGGAAG20150.12709902622592215No Hit
GTATCAACGCAGAGTACTTTTTTTT19800.12489135083241978No Hit
GCTTCCCATGTACTCTGCGTTGATA15910.10035461574463629No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC3200.013.9550024
GCGTTTA1205.2750693E-1113.45632313
CCAACGA500.001499119813.300529519
GTCTTAA2400.012.2825621
GGACGTG2100.012.2143866
GTAGGAC4550.012.1107553
TAGGACG2100.011.7634884
GACGTGA1302.6175258E-911.69129857
AGGACGT2450.011.6338515
GGCGAGA2250.011.400454519
GTACTAA1104.906833E-711.2380041
GTCCTAC4800.011.0939271
ATTATAC1053.4627847E-610.8579193
ACGTGAA1502.1354936E-910.7657388
GTGTTAC1951.8189894E-1210.7281921
TGTAGGA5750.010.5740012
ATAGGAC1659.276846E-1010.3643773
TGGCGAG3700.010.27067918
AGGACTG3450.010.1894555
TAGCCCT4300.010.1641574
Produced by FastQC (version 0.11.5)