##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513060_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1585378
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.35510647933805	31.0	30.0	34.0	25.0	34.0
2	29.049175023243667	31.0	30.0	34.0	16.0	34.0
3	29.390767375351494	31.0	30.0	34.0	23.0	34.0
4	32.23707406057105	35.0	33.0	37.0	19.0	37.0
5	32.4490708209651	35.0	33.0	37.0	22.0	37.0
6	32.92636708721832	37.0	33.0	37.0	26.0	37.0
7	32.915987859046865	37.0	35.0	37.0	26.0	37.0
8	32.907239787609015	37.0	35.0	37.0	26.0	37.0
9	34.287311921825584	39.0	35.0	39.0	26.0	39.0
10	33.77764671895283	38.0	34.0	39.0	21.0	39.0
11	34.175123535207376	39.0	35.0	39.0	26.0	39.0
12	34.00320680620016	39.0	34.0	39.0	25.0	39.0
13	34.10312619451008	39.0	35.0	39.0	25.0	39.0
14	34.40994072076186	40.0	34.0	41.0	17.0	41.0
15	34.97014844409346	40.0	34.0	41.0	24.0	41.0
16	34.54958628163126	39.0	34.0	41.0	17.0	41.0
17	34.084229754670496	39.0	33.0	41.0	16.0	41.0
18	33.49249705748408	39.0	32.0	41.0	10.0	41.0
19	34.39988570549106	39.0	34.0	41.0	18.0	41.0
20	34.657137919158714	39.0	34.0	41.0	18.0	41.0
21	34.683760592111156	40.0	34.0	41.0	18.0	41.0
22	34.27190613216533	40.0	34.0	41.0	11.0	41.0
23	32.58819095509084	39.0	31.0	41.0	2.0	41.0
24	33.68115742744002	39.0	33.0	41.0	2.0	41.0
25	33.4283767025908	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5668674536390235
1101	2	0.9321650572227895
1101	3	0.7388518848790291
1101	4	0.7604137699833657
1101	5	0.8910636718913523
1101	6	0.5162025266734744
1101	7	0.5468345992554049
1101	8	0.5986092617701502
1101	9	0.7358822493370525
1101	10	0.9685985169357636
1101	11	0.62802627250759
1101	12	0.75183006038705
1101	13	0.7230273081251966
1101	14	-0.07913530763187993
1101	15	0.5179185897522487
1101	16	1.0121025975117206
1101	17	1.5996534862318583
1101	18	-4.927483782704741
1101	19	-1.0730343521384995
1101	20	0.056495240405489255
1101	21	0.3671562956778871
1101	22	1.0546235192565874
1101	23	2.720968946228858
1101	24	1.7297895918266093
1101	25	1.8464882042485797
1102	1	0.9005108249442308
1102	2	1.2513303119551047
1102	3	1.0120670581442823
1102	4	1.4223626403321958
1102	5	1.2672634704530807
1102	6	0.8068444608126981
1102	7	0.8684272750317277
1102	8	0.8809987247650284
1102	9	0.9628302175687082
1102	10	1.316098546315061
1102	11	0.9474362394852633
1102	12	1.094639253272149
1102	13	0.9681036839230188
1102	14	1.9287615012236614
1102	15	1.2970474694609706
1102	16	1.5611039779929499
1102	17	1.3851239731746645
1102	18	1.8275392126422716
1102	19	1.3835157443984087
1102	20	1.3384984520808914
1102	21	1.384570440736944
1102	22	1.610261509657505
1102	23	3.2647904113639257
1102	24	2.0425173245676618
1102	25	2.238971736289642
1103	1	0.706964036067042
1103	2	0.9935149626986188
1103	3	0.750596059059486
1103	4	1.0984548624856032
1103	5	1.0852970921096698
1103	6	0.7273838798998327
1103	7	0.6201348505703521
1103	8	0.6405932142814805
1103	9	0.7265424310517758
1103	10	1.1278028188665488
1103	11	0.7799121724070943
1103	12	0.9242020203496182
1103	13	0.7938976050504394
1103	14	1.4606195003015827
1103	15	1.018456331100218
1103	16	1.2355020429929837
1103	17	1.7183289716952004
1103	18	1.772237574223027
1103	19	1.2090973797899167
1103	20	1.0767030033219243
1103	21	1.1095448081805017
1103	22	1.3380000644630101
1103	23	2.918628413607209
1103	24	1.7750909708590257
1103	25	1.999324880664581
1104	1	0.6013366669159304
1104	2	0.9196495501268096
1104	3	0.6649009059219999
1104	4	1.2363810961524493
1104	5	1.155522531945401
1104	6	0.6319782607303566
1104	7	0.4720791132742974
1104	8	0.5813720809837974
1104	9	0.6105680960369355
1104	10	1.0152496277406442
1104	11	0.6636694688373339
1104	12	0.7537198281663038
1104	13	0.5196111049624648
1104	14	1.4410665192923702
1104	15	0.8149311538249293
1104	16	1.2607563610618229
1104	17	1.6190867583977209
1104	18	2.169183536651424
1104	19	1.3287443154173388
1104	20	0.7808120658427384
1104	21	0.9322855839723232
1104	22	1.2085635700977235
1104	23	2.75311010014385
1104	24	1.687732991805781
1104	25	1.8049107389571049
1105	1	0.5719538963548949
1105	2	0.9630703282467152
1105	3	0.6378778172668724
1105	4	0.9504074157722116
1105	5	0.8620259002812745
1105	6	0.47757367015313434
1105	7	0.3619048550410042
1105	8	0.35476790290817917
1105	9	0.4737178455220672
1105	10	0.8888122684323179
1105	11	0.6140190023064136
1105	12	0.6044422456841119
1105	13	0.48618292448112044
1105	14	1.268262616431457
1105	15	0.5556985673784567
1105	16	0.9739100486909393
1105	17	1.4466361922241688
1105	18	1.1513883542074197
1105	19	0.8231100710038106
1105	20	0.6473644729257302
1105	21	0.41335812188602716
1105	22	0.8573635999163045
1105	23	2.3457535459844223
1105	24	1.297418566004879
1105	25	1.2483774592792258
1106	1	-0.46701091526955807
1106	2	-0.09661623798855601
1106	3	-0.2705543368285426
1106	4	-2.797145688917496
1106	5	-0.4348699913420049
1106	6	-0.5224543705729445
1106	7	-0.5076486613686342
1106	8	-0.4178402744514713
1106	9	-0.5132250929682058
1106	10	-0.3171825994308932
1106	11	-0.3500863036144892
1106	12	-0.3632196159875676
1106	13	-0.31849614940892934
1106	14	0.43803621834479145
1106	15	-0.14795968729836062
1106	16	-1.0760434789349915
1106	17	-5.998351664773601
1106	18	-7.176307026390724
1106	19	-3.0604684249053236
1106	20	-1.5894716030413818
1106	21	-1.1260698746588105
1106	22	-1.2249641428257618
1106	23	-4.783207634024045
1106	24	-2.005471030817354
1106	25	-0.6969178198251669
1107	1	0.4908962763280904
1107	2	0.8561239006459544
1107	3	0.6222423145483482
1107	4	0.7951508030783359
1107	5	0.7693545522608716
1107	6	0.3590289268321172
1107	7	0.32697998075875745
1107	8	0.3908362684382425
1107	9	0.2587877277524129
1107	10	0.6655245554280711
1107	11	0.366121534692077
1107	12	0.5050809766938755
1107	13	0.3261536444762356
1107	14	1.222315978030565
1107	15	0.6967801974070014
1107	16	-5.777075673909351
1107	17	-1.0569154253022077
1107	18	0.6990232165708505
1107	19	0.5693764892180013
1107	20	0.46787181181941406
1107	21	0.5089042731927904
1107	22	0.6132964965354191
1107	23	2.457554342517227
1107	24	1.4668398865152028
1107	25	1.1756495929609727
1108	1	0.12603211104857337
1108	2	0.5562338772568403
1108	3	0.2359745238238169
1108	4	0.6672480709858704
1108	5	0.43379613390639093
1108	6	0.012135683126068386
1108	7	0.0033746328556745198
1108	8	0.10819505103631855
1108	9	0.1855112477473142
1108	10	0.5016215737809588
1108	11	0.07685749525182217
1108	12	0.21637777150950654
1108	13	0.08085038247836707
1108	14	0.6785108403905582
1108	15	0.32176460024130193
1108	16	0.5504840532796251
1108	17	0.7566118305789189
1108	18	0.6128551813358527
1108	19	0.4596139245395392
1108	20	0.35574677431984725
1108	21	0.3359867934823768
1108	22	0.6943267590191198
1108	23	2.3612856491482077
1108	24	1.1674015172221033
1108	25	1.471185020429509
1109	1	0.0862081422163925
1109	2	0.2640834007448092
1109	3	0.20399394567874296
1109	4	0.304788000135531
1109	5	0.04679760577860037
1109	6	0.03112098814388986
1109	7	-0.016371667021140013
1109	8	-0.11203316828846255
1109	9	-0.11545133821081066
1109	10	0.5034469063370324
1109	11	0.131059255167834
1109	12	0.1439325776470568
1109	13	-0.019921756932724577
1109	14	-1.1036161336459287
1109	15	-0.3101163363758701
1109	16	-0.3900112923861343
1109	17	0.543116833097983
1109	18	1.2690681702124067
1109	19	0.5315196957029755
1109	20	0.342609061946888
1109	21	0.16947696385554423
1109	22	-0.026915680490361638
1109	23	-4.6313045425908435
1109	24	-1.6335656848226847
1109	25	-0.3466958528813322
1110	1	0.6029287828494603
1110	2	0.9708929386731278
1110	3	0.7861312891883223
1110	4	1.2781347864906394
1110	5	1.099944922757011
1110	6	0.6260018534995027
1110	7	0.6363154441490408
1110	8	0.4531841674509067
1110	9	0.4845255300893001
1110	10	0.8996520295612527
1110	11	0.6430308683546926
1110	12	0.5788181204624294
1110	13	0.44167514827586984
1110	14	1.3800951878514667
1110	15	0.9222626758877652
1110	16	1.2274133953998216
1110	17	1.4935300087097616
1110	18	2.2116414887550846
1110	19	1.3676609958834334
1110	20	0.8922367821581147
1110	21	0.7562764311874446
1110	22	1.0732202981211287
1110	23	2.4615398385786733
1110	24	1.5456455625510301
1110	25	1.7924470729080824
1111	1	0.43832649717689165
1111	2	0.7291982958676861
1111	3	0.5379067827201389
1111	4	0.9968723168112419
1111	5	0.6725371810765282
1111	6	0.3707795360304331
1111	7	0.20450421416818898
1111	8	0.008277020538727697
1111	9	0.18209119691583453
1111	10	0.4939228058566201
1111	11	0.23634403621026934
1111	12	0.5000084878593043
1111	13	0.3650510531733602
1111	14	-0.41024615251082963
1111	15	0.011641561550007395
1111	16	0.6307547345033484
1111	17	1.1296328224358803
1111	18	1.819140982100599
1111	19	0.9258161190099088
1111	20	0.6488075558100732
1111	21	0.6602122239034571
1111	22	0.9168944179584457
1111	23	0.8963318333119901
1111	24	0.6416298380099477
1111	25	0.475428013888191
1112	1	0.37149489778816047
1112	2	0.7510275243880784
1112	3	0.48553985264825883
1112	4	1.1123775743185433
1112	5	0.8180118592853205
1112	6	0.08880610683746681
1112	7	0.15428656560774812
1112	8	0.16432523378339425
1112	9	0.22736766483762239
1112	10	0.6810877566093083
1112	11	0.444780057086561
1112	12	0.4609176296682307
1112	13	0.31671238499085774
1112	14	0.7476233114957793
1112	15	0.4172677159446252
1112	16	0.749829580238746
1112	17	1.069664878979907
1112	18	1.089265605316001
1112	19	0.6563266797337164
1112	20	0.47061188942208787
1112	21	0.40591911751552345
1112	22	0.2710001324312401
1112	23	1.9413361021403048
1112	24	0.8138464559781227
1112	25	1.0679673230117785
1113	1	0.40540458510235666
1113	2	0.8342629430424537
1113	3	0.512024226166691
1113	4	0.6885544508580779
1113	5	0.9412168652924748
1113	6	0.4138448596958284
1113	7	0.17128937723180115
1113	8	0.10513945575969785
1113	9	0.3565455592223543
1113	10	0.5810181313443081
1113	11	0.3296929521987835
1113	12	0.32411760939738343
1113	13	0.14211280994560838
1113	14	0.8426249059127571
1113	15	0.2893572276769376
1113	16	0.7193734271836405
1113	17	1.1997860371401714
1113	18	1.7380518006131993
1113	19	0.7287659592197926
1113	20	0.4559830937574958
1113	21	0.21626540958370555
1113	22	0.4627261840253283
1113	23	0.5778716611448047
1113	24	0.5653062518895666
1113	25	0.8249604868593536
1114	1	-0.4745182353617139
1114	2	-3.1684012483120974
1114	3	-1.2054880789428672
1114	4	-0.0604317211935097
1114	5	0.0632291250461634
1114	6	-0.16083466691316062
1114	7	-0.2818803767405669
1114	8	-0.26719914879551965
1114	9	-0.07791039401512023
1114	10	-0.09518227698278281
1114	11	-0.18762056372609948
1114	12	-0.09041096293653084
1114	13	-0.2712815549914467
1114	14	0.5161187182537788
1114	15	-0.008418967823018875
1114	16	0.33550107351223346
1114	17	0.6215431235288094
1114	18	0.869440299110245
1114	19	0.2282058452957898
1114	20	0.11704535928939919
1114	21	0.04668820084605585
1114	22	0.15259268865349185
1114	23	1.810558455876567
1114	24	0.7379246338158083
1114	25	0.6653019233605164
1115	1	0.4332769064478583
1115	2	0.7679261277186207
1115	3	0.5210437818283005
1115	4	0.7446397712104229
1115	5	0.6677336714714812
1115	6	0.3364308261899467
1115	7	0.17914421249429324
1115	8	0.22743280611367567
1115	9	0.21163518418208582
1115	10	0.46292886554744683
1115	11	0.3420872027209185
1115	12	0.2143237682689758
1115	13	0.17903692147584138
1115	14	1.2338595905040606
1115	15	0.45972920949055407
1115	16	0.8725616583134226
1115	17	1.3652493579957365
1115	18	1.717258241651443
1115	19	0.9977063768356089
1115	20	0.5475140151439106
1115	21	0.5516602756171807
1115	22	0.7879456346726741
1115	23	2.5581325224604328
1115	24	1.2813156729254516
1115	25	0.9462171382851494
1116	1	0.5753348181855706
1116	2	0.5856186188483115
1116	3	0.710281332683536
1116	4	1.376140605850516
1116	5	0.845051261059794
1116	6	0.6779765362843975
1116	7	0.5940062981551009
1116	8	0.5511482913972756
1116	9	0.5167827817916191
1116	10	1.0611293670541286
1116	11	0.5588427290500135
1116	12	0.6926370003856377
1116	13	0.5196362725521979
1116	14	1.2708160085611908
1116	15	0.5589918356801888
1116	16	1.0578507472911127
1116	17	1.4244215106445495
1116	18	2.009787092738641
1116	19	1.1765130221605418
1116	20	0.7948443953206663
1116	21	0.6998916335151932
1116	22	1.077755799216007
1116	23	2.381318462929997
1116	24	1.4569339208316237
1116	25	1.5447535191989914
1201	1	0.9943578649121783
1201	2	1.3651348908503351
1201	3	1.0472887405599458
1201	4	1.6137852810436257
1201	5	1.4394339380257364
1201	6	0.9536565413496092
1201	7	0.9391544556832798
1201	8	0.9530281191799759
1201	9	1.1006867959742195
1201	10	1.3840105244556753
1201	11	1.0316384109545353
1201	12	1.132578750417295
1201	13	0.9577374502146014
1201	14	1.903547108979069
1201	15	1.4230269122915615
1201	16	1.7860161898642417
1201	17	2.221674581430925
1201	18	2.736986662195399
1201	19	1.8701450976322391
1201	20	1.4853195757689406
1201	21	1.4125214296403001
1201	22	1.613994244195112
1201	23	3.352246030360668
1201	24	2.2640084428019875
1201	25	2.4140200128041585
1202	1	0.7589187022590309
1202	2	1.098334704127243
1202	3	0.7691274109685295
1202	4	1.5342336959937697
1202	5	1.2139672001732151
1202	6	0.818610391674838
1202	7	0.7013651816391828
1202	8	0.48196705401976914
1202	9	0.7475055152438728
1202	10	1.2799220084849807
1202	11	0.9484643489725357
1202	12	0.9631750623566688
1202	13	0.7943837441245805
1202	14	1.629835127953271
1202	15	1.2796690692228125
1202	16	1.5101445242551108
1202	17	1.9255788900383521
1202	18	2.5247390388617177
1202	19	1.7821348935506052
1202	20	1.3128430279658687
1202	21	1.2804618361823188
1202	22	1.736531623754523
1202	23	3.3525133267053135
1202	24	2.01347262718825
1202	25	2.070700569563229
1203	1	0.9067100207915182
1203	2	1.285798643082341
1203	3	1.0112264599122014
1203	4	1.5035452040219468
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2211	14	-0.2160392113125198
2211	15	-0.5813923288753386
2211	16	-0.5649170524593359
2211	17	-0.029103652890071885
2211	18	-5.274267053289261
2211	19	-1.9477657240110489
2211	20	-1.2767433083886388
2211	21	-1.0650533507930504
2211	22	-0.7784244858824962
2211	23	-5.442467221646702
2211	24	-2.735980965544904
2211	25	-1.8428798607684875
2212	1	-0.002965176516575241
2212	2	0.1883809193273862
2212	3	-0.002623328670139813
2212	4	0.4552622218470006
2212	5	0.3325776935613689
2212	6	-0.033809369913335274
2212	7	-0.2705289666240134
2212	8	-0.35154044461071976
2212	9	-0.539027222068249
2212	10	-4.066731587013486
2212	11	-1.2979762887280657
2212	12	-0.675506707657263
2212	13	-0.28244411858070606
2212	14	0.41993679203794443
2212	15	0.08280280829028897
2212	16	0.3127593593293483
2212	17	0.7615885216716478
2212	18	1.2389778078730984
2212	19	0.48825079358089596
2212	20	0.1074694814107815
2212	21	-0.04327678522709988
2212	22	0.2083675498536408
2212	23	1.5697008770858574
2212	24	0.7786381644117597
2212	25	0.7388639718626777
2213	1	-1.8602270358914232
2213	2	-4.1143340417325795
2213	3	-2.086573611195128
2213	4	-3.7825485143750264
2213	5	-1.6761950923726872
2213	6	-1.388221460082228
2213	7	-1.3942551842923763
2213	8	-1.339869732257359
2213	9	-1.3359563463489579
2213	10	-1.3972182761513778
2213	11	-1.4769139101527529
2213	12	-1.2194659100840681
2213	13	-1.6065796645496917
2213	14	-6.603155753626073
2213	15	-3.2499316448224533
2213	16	-2.606143252362134
2213	17	-1.1852292480676425
2213	18	-0.4872425848557924
2213	19	-1.080788781712549
2213	20	-1.210171170905518
2213	21	-1.4638371749335377
2213	22	-1.2383886010885377
2213	23	-6.147756986153745
2213	24	-3.594463571988314
2213	25	-2.8133750661212638
2214	1	0.1961552076455071
2214	2	0.38812612690979265
2214	3	0.25415556619716284
2214	4	0.6099991222428045
2214	5	0.216061067658039
2214	6	-0.008857533712138377
2214	7	0.01660633800132416
2214	8	0.016060445233208043
2214	9	0.14561609913726414
2214	10	0.6402952527840213
2214	11	0.07518071356901856
2214	12	0.1584427426691164
2214	13	0.10582645709924066
2214	14	0.7401072623152203
2214	15	0.43112515819108665
2214	16	0.8013457037923217
2214	17	1.1312157344179852
2214	18	1.5336709188615316
2214	19	0.5596005141359512
2214	20	0.4396755285029812
2214	21	0.45041074125494873
2214	22	0.9348141149013998
2214	23	2.3282065466956112
2214	24	1.2928378766761028
2214	25	1.0722908012438452
2215	1	-2.2865335875574004
2215	2	-4.228870826749294
2215	3	-2.3023223259844308
2215	4	-3.865262960883907
2215	5	-4.658149750209244
2215	6	-2.2183744200399858
2215	7	-1.7427794751281454
2215	8	-2.150764644658633
2215	9	-1.6506991726581859
2215	10	-2.9611319109658503
2215	11	-1.9766986374560034
2215	12	-1.6718144507998076
2215	13	-1.7051205703922605
2215	14	-0.9370973153393791
2215	15	-1.6378595839030936
2215	16	-2.3594056108475243
2215	17	-6.687234226942628
2215	18	-2.9336819622850214
2215	19	-2.0491306922421444
2215	20	-1.8228430449763877
2215	21	-1.8515293862289752
2215	22	-1.6241755235497592
2215	23	-6.252941005148234
2215	24	-3.535905748494894
2215	25	-3.0678386477753143
2216	1	-0.7481427206812086
2216	2	-0.2480934090956879
2216	3	-0.5056673603046953
2216	4	-3.1469896372279074
2216	5	-0.9241762706491947
2216	6	-0.7090887713572585
2216	7	-0.6432983658584135
2216	8	-0.5498603838396505
2216	9	-0.5323141918963614
2216	10	-0.1597046581985353
2216	11	-0.8742268160010909
2216	12	-1.41999735126209
2216	13	-0.8301873595948521
2216	14	-6.142980194948986
2216	15	-2.8796141138717317
2216	16	-1.2956963652579176
2216	17	-0.21529074634860734
2216	18	0.24522443347790102
2216	19	-0.16504079007641081
2216	20	-0.8379706188517488
2216	21	-0.9485408112982796
2216	22	-0.3287334497890626
2216	23	0.7864316991083129
2216	24	-0.006146790366017285
2216	25	0.009639137774883011
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73580.0
3	1.0
4	603.0
5	3670.0
6	4391.0
7	4071.0
8	3763.0
9	3580.0
10	3403.0
11	3164.0
12	3226.0
13	3336.0
14	3187.0
15	3288.0
16	3517.0
17	3683.0
18	4096.0
19	5043.0
20	6143.0
21	7465.0
22	9279.0
23	11367.0
24	13683.0
25	16225.0
26	18171.0
27	21172.0
28	23453.0
29	28575.0
30	33615.0
31	38448.0
32	49194.0
33	65065.0
34	84138.0
35	111158.0
36	157388.0
37	218890.0
38	542347.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3347924209952	17.34165257464143	12.103334221583468	25.2202207827799
2	14.413488838501099	18.78065049895904	41.427641946006524	25.37821871653333
3	20.3330624277515	22.91817052917451	28.02023967572097	28.728527367353017
4	11.119671143332166	15.363764563359487	37.992220255728334	35.524344037580015
5	13.078580645568383	37.37854183953231	35.577247674004056	13.965629840895252
6	34.443491098128774	34.801837154429485	17.16666876963648	13.588002977805255
7	29.941459001427724	30.452107608437124	20.28348757730863	19.32294581282653
8	26.616992482626127	36.03569727349298	19.14081747891949	18.206492764961403
9	26.667936995076	15.458024900119376	18.911067757298287	38.96297034750633
10	14.727399616601483	29.013789978886578	33.68284689173346	22.575963512778475
11	35.97344931749529	22.474013339692778	21.799521022760253	19.75301632005168
12	23.636537993219125	24.602188201478064	30.21931224708936	21.54196155821345
13	29.45394005118859	20.592018643395228	24.75650093147581	25.19754037394038
14	21.271030372314957	20.87740981395293	27.46978130999496	30.381778503737156
15	25.192756968808734	28.026967363962886	22.442898449048503	24.337377218179878
16	23.187981537234673	27.598502991745256	26.418776198275363	22.794739272744707
17	23.045803650689198	27.350893807481697	27.07700694212665	22.526295599702454
18	22.577659618764738	26.735299276114816	28.47346910964772	22.21357199547272
19	24.000471976996803	26.742039962822346	27.052926415126482	22.20456164505437
20	23.88107537898958	26.48813504571597	27.146997736283318	22.483791839011133
21	23.69389906801044	26.144102205264847	27.09950034355718	23.06249838316753
22	23.36210524854505	26.48818782366827	26.54226531033347	23.60744161745321
23	21.88625268865713	26.134536017752552	28.48642090126879	23.49279039232153
24	23.184882990840567	27.07235731571491	26.643255369295865	23.099504324148658
25	23.37363400778755	26.027408496899053	26.522033496189195	24.0769239991242
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	148.0
2	248.0
3	348.0
4	348.0
5	348.0
6	658.5
7	969.0
8	969.0
9	969.0
10	1577.5
11	2186.0
12	2186.0
13	2186.0
14	3741.0
15	5296.0
16	5296.0
17	5296.0
18	9494.5
19	13693.0
20	13693.0
21	13693.0
22	21472.5
23	29252.0
24	29252.0
25	29252.0
26	41516.0
27	53780.0
28	53780.0
29	53780.0
30	69860.0
31	85940.0
32	85940.0
33	85940.0
34	103760.5
35	121581.0
36	121581.0
37	121581.0
38	138835.5
39	156090.0
40	156090.0
41	156090.0
42	173366.5
43	190643.0
44	190643.0
45	190643.0
46	212104.0
47	233565.0
48	233565.0
49	233565.0
50	226872.0
51	220179.0
52	220179.0
53	220179.0
54	197888.5
55	175598.0
56	175598.0
57	175598.0
58	152448.5
59	129299.0
60	129299.0
61	129299.0
62	106321.0
63	83343.0
64	83343.0
65	83343.0
66	65079.5
67	46816.0
68	46816.0
69	46816.0
70	34407.5
71	21999.0
72	21999.0
73	21999.0
74	15593.0
75	9187.0
76	9187.0
77	9187.0
78	6404.0
79	3621.0
80	3621.0
81	3621.0
82	2425.0
83	1229.0
84	1229.0
85	1229.0
86	839.0
87	449.0
88	449.0
89	449.0
90	284.0
91	119.0
92	119.0
93	119.0
94	75.0
95	31.0
96	31.0
97	31.0
98	24.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10975300527697494
2	0.04913654661538132
3	0.03595357069418145
4	0.03109668482847624
5	0.03614280001362451
6	0.020058307860964388
7	0.021130607337808395
8	0.02415827644889736
9	0.030655149749775762
10	0.03809816964786947
11	0.021067530897994045
12	0.020941378018365337
13	0.016336797911917538
14	0.022013677495209345
15	0.022139830374838052
16	0.022076753935023695
17	0.026302875402585375
18	0.020184460740593095
19	0.03500742409696615
20	0.023590588490568182
21	0.030844379069218827
22	0.03879201048582735
23	0.030213614671075292
24	0.04245044399505986
25	0.0341243539395652
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1585378.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.81545150648988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.5166266750656	64.83481662682034
2	10.134146672187674	15.366498111698004
3	2.4152549462270665	5.493409327544638
4	0.8313459714016915	2.521154807196826
5	0.38163302389405396	1.4466840008157367
6	0.1987713377172253	0.9041963229348247
7	0.12423927931065895	0.6593479939044959
8	0.08491713915936132	0.5150424996805127
9	0.053507204131531004	0.36510055560737487
>10	0.23527658074794466	3.055547800151425
>50	0.014160664141547223	0.7204115003209095
>100	0.007421919117246188	1.201358592372316
>500	0.0015179551303306188	0.7320010605293307
>1k	0.0010119700868870792	1.3374092221287919
>5k	1.6866168114784653E-4	0.8470215782943988
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7430	0.46865794782064596	No Hit
CCCATGTACTCTGCGTTGATACCAC	5818	0.3669787268399082	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3416	0.2154691184058313	No Hit
GCGTTGATACCACTGCTTCCCATGT	2069	0.13050515397589724	No Hit
CATGTACTCTGCGTTGATACCACTG	2031	0.12810824926295178	No Hit
GTATCAACGCAGAGTACATGGGAAG	2015	0.12709902622592215	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1980	0.12489135083241978	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1591	0.10035461574463629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.307643981435342E-5
2	0.0	0.0	0.0	0.0	6.307643981435342E-5
3	0.0	0.0	0.0	0.0	6.307643981435342E-5
4	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
5	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
6	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
7	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
8	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
9	0.0	0.0	0.0	6.307643981435342E-5	6.307643981435342E-5
10	0.0	0.0	0.0	1.2615287962870685E-4	6.307643981435342E-5
11	6.307643981435342E-5	0.0	0.0	1.2615287962870685E-4	6.307643981435342E-5
12	6.307643981435342E-5	0.0	0.0	1.2615287962870685E-4	2.523057592574137E-4
13	6.307643981435342E-5	0.0	0.0	1.8922931944306026E-4	3.153821990717671E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	320	0.0	13.955002	4
GCGTTTA	120	5.2750693E-11	13.456323	13
CCAACGA	50	0.0014991198	13.3005295	19
GTCTTAA	240	0.0	12.282562	1
GGACGTG	210	0.0	12.214386	6
GTAGGAC	455	0.0	12.110755	3
TAGGACG	210	0.0	11.763488	4
GACGTGA	130	2.6175258E-9	11.6912985	7
AGGACGT	245	0.0	11.633851	5
GGCGAGA	225	0.0	11.4004545	19
GTACTAA	110	4.906833E-7	11.238004	1
GTCCTAC	480	0.0	11.093927	1
ATTATAC	105	3.4627847E-6	10.857919	3
ACGTGAA	150	2.1354936E-9	10.765738	8
GTGTTAC	195	1.8189894E-12	10.728192	1
TGTAGGA	575	0.0	10.574001	2
ATAGGAC	165	9.276846E-10	10.364377	3
TGGCGAG	370	0.0	10.270679	18
AGGACTG	345	0.0	10.189455	5
TAGCCCT	430	0.0	10.164157	4
>>END_MODULE