FastQCFastQC Report
Thu 26 May 2016
SRR1513060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1585378
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC84430.5325543813525859No Hit
CCCATGTACTCTGCGTTGATACCAC78440.4947715939037882No Hit
GAGTACATGGGAAGCAGTGGTATCA35890.22638134249371444No Hit
GTATCAACGCAGAGTACTTTTTTTT30550.1926985236328497No Hit
CATGTACTCTGCGTTGATACCACTG24490.15447420110535154No Hit
TATCAACGCAGAGTACTTTTTTTTT22350.1409758429850799No Hit
GTATCAACGCAGAGTACATGGGAAG22320.14078661366563683No Hit
GCGTTGATACCACTGCTTCCCATGT20670.13037900109626852No Hit
GGTATCAACGCAGAGTACTTTTTTT19670.12407135711483318No Hit
ACGCAGAGTACATGGGAAGCAGTGG19540.12325136339724659No Hit
TATCAACGCAGAGTACATGGGAAGC19380.12224214036021692No Hit
GCTTCCCATGTACTCTGCGTTGATA19250.12142214664263033No Hit
ACTCTGCGTTGATACCACTGCTTCC17020.10735610056402953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG959.993746E-712.0394571
TCGAACT1001.9658983E-611.38228419
TAGGACC2600.011.3530124
CTATACT2400.011.1088614
CTTACAC2600.010.9867863
TCTATAC1657.6397555E-1110.964593
TAGGACG2800.010.8821494
TATACCG700.001463074110.8821495
CTCGAAC1158.9568675E-710.72582518
GGAGTGT1701.3278623E-1010.6421026
GTAGGAC3950.010.3655933
TATACTT1752.2555469E-1010.3380415
TAGGGGC750.002597559510.1566734
AGCCGTG750.002677085810.1198227
CCGCCGG856.6880253E-410.04319319
ACTAGAC951.6061375E-410.0230323
GCCGCCG951.6645585E-49.98761218
ATTAGAC1054.0018713E-59.9753043
TTACACA3450.09.9358764
CCTACAC3650.09.9131913
Produced by FastQC (version 0.11.5)