FastQCFastQC Report
Thu 26 May 2016
SRR1513059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072156
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC198740.959097674113339No Hit
CCCATGTACTCTGCGTTGATACCAC157150.758388847171738No Hit
GAGTACATGGGAAGCAGTGGTATCA81000.39089721044168485No Hit
GTATCAACGCAGAGTACATGGGAAG53310.25726827516847184No Hit
GCGTTGATACCACTGCTTCCCATGT52240.25210457127745206No Hit
CATGTACTCTGCGTTGATACCACTG51320.24766475110947245No Hit
GCTTCCCATGTACTCTGCGTTGATA42570.2054382005987966No Hit
ACGCAGAGTACATGGGAAGCAGTGG42410.2046660579608871No Hit
TATCAACGCAGAGTACATGGGAAGC40830.19704114941153078No Hit
ACTCTGCGTTGATACCACTGCTTCC35760.17257387957277348No Hit
GGTATCAACGCAGAGTACATGGGAA26960.13010603448775093No Hit
GTATCAACGCAGAGTACTTTTTTTT22670.10940296000880242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG1900.014.00049818
TAGGACC5300.013.0863194
GCGTTTA2700.013.01646813
GTATAAT3250.012.2921981
GATATAC5050.012.2429371
ATACGAC701.0911216E-412.21442619
GTAGGAC10850.012.0839633
CGTTTAT3000.011.7148214
CGGTTTC1552.5465852E-1111.64332713
GTATTAG1800.011.6255581
TGCGTTT3550.011.50522912
GTCCTAG3250.011.4141841
CCAACGA1501.7644197E-1011.4001319
CCGGTTT1501.7644197E-1011.39820412
TGTAGGA12300.011.2773692
TAGGACA5200.011.1454344
AGGACCT8900.011.1012515
ACTGTTC6850.010.8160758
CGCAGAT1855.456968E-1210.7823468
TACACTG11950.010.6528425
Produced by FastQC (version 0.11.5)