##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513059_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2072156
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.3586655637896	31.0	30.0	34.0	25.0	34.0
2	29.0833455589251	31.0	30.0	34.0	16.0	34.0
3	29.424670729423845	31.0	30.0	34.0	23.0	34.0
4	32.2575143956343	35.0	33.0	37.0	19.0	37.0
5	32.517151218344566	35.0	33.0	37.0	23.0	37.0
6	32.94491679197898	37.0	33.0	37.0	26.0	37.0
7	32.94770857020417	37.0	35.0	37.0	26.0	37.0
8	32.92785533521607	37.0	35.0	37.0	26.0	37.0
9	34.300745696752564	39.0	35.0	39.0	26.0	39.0
10	33.808544337395446	39.0	34.0	39.0	22.0	39.0
11	34.19004505452292	39.0	35.0	39.0	26.0	39.0
12	34.01838133808459	39.0	35.0	39.0	25.0	39.0
13	34.112915726422145	39.0	35.0	39.0	25.0	39.0
14	34.42251548628578	40.0	34.0	41.0	17.0	41.0
15	34.96988112864089	40.0	34.0	41.0	24.0	41.0
16	34.55267460557989	39.0	34.0	41.0	17.0	41.0
17	34.10121824804696	39.0	33.0	41.0	16.0	41.0
18	33.505275181984366	39.0	32.0	41.0	10.0	41.0
19	34.3917784182272	39.0	34.0	41.0	18.0	41.0
20	34.66103951632985	39.0	34.0	41.0	18.0	41.0
21	34.68156017211059	40.0	34.0	41.0	18.0	41.0
22	34.27918843948043	40.0	34.0	41.0	10.0	41.0
23	32.618360297197704	39.0	31.0	41.0	2.0	41.0
24	33.71187787019896	39.0	33.0	41.0	2.0	41.0
25	33.444953468754285	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9914975171717799
1101	2	1.3625241245741222
1101	3	1.116948180969974
1101	4	1.0732337845634063
1101	5	1.2652609234322298
1101	6	1.1038279949764132
1101	7	1.063475784776223
1101	8	1.089845236749973
1101	9	1.2775982608368324
1101	10	1.534072183016697
1101	11	1.2006662930519454
1101	12	1.2490288879545872
1101	13	1.1603463853725131
1101	14	0.30449335975414016
1101	15	0.973950322251703
1101	16	1.5143983268773127
1101	17	2.2227737900215274
1101	18	-4.203428759063609
1101	19	-0.2637451316782773
1101	20	0.6648184249944791
1101	21	1.0518285553061233
1101	22	1.5804585588462459
1101	23	3.4129987372294934
1101	24	2.359662556440071
1101	25	2.5972394868114748
1102	1	1.0501579737095632
1102	2	1.3025646605024725
1102	3	1.128260327793761
1102	4	1.6439312345491999
1102	5	1.4543450148877426
1102	6	1.0722888654480656
1102	7	0.9812810357845763
1102	8	0.9225416409727032
1102	9	1.0647323068134185
1102	10	1.503338602085222
1102	11	1.0566193665361396
1102	12	1.1636026974743885
1102	13	1.1700869453433214
1102	14	2.0501028782155544
1102	15	1.3805377888990904
1102	16	1.6079684812702766
1102	17	1.3942993179048955
1102	18	1.772799886437845
1102	19	1.5153641118920476
1102	20	1.5892338821123033
1102	21	1.57897447119786
1102	22	1.8914833309892742
1102	23	3.4643304458751984
1102	24	2.413369929405924
1102	25	2.6784420638944653
1103	1	0.8607041333066832
1103	2	1.0974364017672116
1103	3	0.9799524365824581
1103	4	1.4106043683708052
1103	5	1.2512056153630766
1103	6	0.921254942990366
1103	7	0.8982751718590904
1103	8	0.9025372254669151
1103	9	1.0347187843269552
1103	10	1.2955827829001905
1103	11	0.9791646631025799
1103	12	1.1224864358690567
1103	13	0.9053221797414537
1103	14	1.7772842049746913
1103	15	1.2931074292862448
1103	16	1.482608676087743
1103	17	2.008495127188496
1103	18	1.9991326081790248
1103	19	1.4851850079386608
1103	20	1.4582501609448073
1103	21	1.3990637262986851
1103	22	1.7227664786427823
1103	23	3.139328940589138
1103	24	2.087789725255682
1103	25	2.2782513106994955
1104	1	0.7062464771415726
1104	2	1.0971716689566087
1104	3	0.8746047080444725
1104	4	1.425351012992472
1104	5	1.2291889843574353
1104	6	0.8182174955596366
1104	7	0.7421559662168207
1104	8	0.8974301669314073
1104	9	0.9301004858609403
1104	10	1.202376082539999
1104	11	0.8121914122255873
1104	12	0.977975229475561
1104	13	0.6873389197423876
1104	14	1.8034164565157198
1104	15	1.1244837380682497
1104	16	1.4586802256319729
1104	17	1.900490847865406
1104	18	2.546470314180141
1104	19	1.6850249525355139
1104	20	1.2439989418743167
1104	21	1.291447637921948
1104	22	1.6004913610955427
1104	23	2.9997818412055466
1104	24	1.9289299166849574
1104	25	2.0007469760403325
1105	1	0.4080186420900276
1105	2	0.7852453802968142
1105	3	0.455440790518697
1105	4	0.8032171985594871
1105	5	0.6818048606460962
1105	6	0.3719403821575753
1105	7	0.18396802807249202
1105	8	0.22095356899326646
1105	9	0.46695883637640634
1105	10	0.838196185632782
1105	11	0.403265639029712
1105	12	0.42922028102194787
1105	13	0.40300087850527433
1105	14	1.0971544977528325
1105	15	0.585909511264127
1105	16	0.9616916689309392
1105	17	1.4180256932262338
1105	18	1.4572739224275608
1105	19	0.9398779422262393
1105	20	0.7732706944516394
1105	21	0.6231057822518409
1105	22	1.0553676497553397
1105	23	2.587949057700392
1105	24	1.5931748651385718
1105	25	1.589696151298071
1106	1	-0.30063983707001185
1106	2	0.1439874738380169
1106	3	-0.07760356201073293
1106	4	-2.521568954797182
1106	5	-0.3453521740341472
1106	6	-0.26986976101773763
1106	7	-0.2624522328247352
1106	8	-0.2261488427152898
1106	9	-0.12013039047692331
1106	10	0.13716048725543573
1106	11	-0.020662073346997545
1106	12	0.003557258432067556
1106	13	-0.03191170183198011
1106	14	0.6639731550865875
1106	15	0.11918498056097349
1106	16	-0.9075376788259817
1106	17	-5.530087167098298
1106	18	-6.722772148205593
1106	19	-2.6556685292977846
1106	20	-1.272129191008112
1106	21	-0.9548315260523239
1106	22	-1.3257688440403967
1106	23	-5.069247149744722
1106	24	-1.9617590960450144
1106	25	-0.5129542082310437
1107	1	0.5422377551443667
1107	2	0.8378779849189257
1107	3	0.6478275487947762
1107	4	0.9928760617699766
1107	5	0.9801721810708273
1107	6	0.5475924995841837
1107	7	0.4218642429382058
1107	8	0.557970333515442
1107	9	0.4795021967761528
1107	10	0.8913339745147297
1107	11	0.6166362823776197
1107	12	0.6897148719026518
1107	13	0.6361428647638618
1107	14	1.3520359968159141
1107	15	0.8224201920886145
1107	16	-5.267454414766746
1107	17	-0.7089043754808273
1107	18	0.9058975326711547
1107	19	0.7849435762050021
1107	20	0.6345505945140957
1107	21	0.7593889052999359
1107	22	0.7166518471319776
1107	23	2.6489923056704114
1107	24	1.6867600743827893
1107	25	1.458848830873336
1108	1	0.4064644426562438
1108	2	0.7432759559495352
1108	3	0.4717744803582704
1108	4	1.0685809481662787
1108	5	0.8972613941471934
1108	6	0.5488426029817361
1108	7	0.32479953483562696
1108	8	0.43462810815652375
1108	9	0.5262025230703316
1108	10	0.9901101208325329
1108	11	0.5173850476487729
1108	12	0.6904418475523073
1108	13	0.5251176699491253
1108	14	1.209307099199826
1108	15	0.8115321598957692
1108	16	1.1054590423187989
1108	17	1.0717670710179945
1108	18	1.0515946522167994
1108	19	1.0227946833397965
1108	20	0.7106486372009329
1108	21	0.7538478772502728
1108	22	1.25991750820139
1108	23	2.847243477329208
1108	24	1.5370338396588608
1108	25	1.7312846256610754
1109	1	0.15237805878254918
1109	2	0.49151605978797264
1109	3	0.2922194439471788
1109	4	0.42691695654893635
1109	5	0.16560623824460663
1109	6	0.07227979264470008
1109	7	0.0636231071913258
1109	8	0.06437408404135425
1109	9	0.21519717332954968
1109	10	0.5892767937618544
1109	11	0.27912887204243475
1109	12	0.2650156021513297
1109	13	0.11753248260084348
1109	14	-1.150473972616389
1109	15	-0.2261994555369924
1109	16	-0.4849041238087395
1109	17	0.6038767884791056
1109	18	1.3256038014222824
1109	19	0.6631059409851545
1109	20	0.4312532448705042
1109	21	0.3596386522487407
1109	22	0.16842008537432918
1109	23	-4.2807659531590545
1109	24	-1.5124794445108236
1109	25	-0.3078759492293912
1110	1	0.6929527066847854
1110	2	0.9760782870609823
1110	3	0.8083714425601372
1110	4	1.4075057055674094
1110	5	1.0724259941013088
1110	6	0.6458103638760377
1110	7	0.6013875527525698
1110	8	0.6477405525398865
1110	9	0.6979026023727499
1110	10	1.075623120428638
1110	11	0.6892268821741325
1110	12	0.8298086052145166
1110	13	0.6112233769818189
1110	14	1.476347965150758
1110	15	0.8171085362488952
1110	16	1.3799987499790802
1110	17	1.6984908729946682
1110	18	2.3277811741033645
1110	19	1.51869164119028
1110	20	0.9695497438117826
1110	21	0.9712718273462997
1110	22	1.240837485221519
1110	23	2.6257795513018536
1110	24	1.6779687225425874
1110	25	2.041488375551573
1111	1	0.5744397658235236
1111	2	0.9191703419179404
1111	3	0.6074329305248369
1111	4	1.118777186278244
1111	5	0.9271430737256168
1111	6	0.45559567230188236
1111	7	0.38914913952474706
1111	8	0.20321297335310362
1111	9	0.3698478262443814
1111	10	0.6384403366920424
1111	11	0.40736904707392085
1111	12	0.6085371001806834
1111	13	0.47029539493143346
1111	14	-0.2746351982864681
1111	15	0.18184294694113134
1111	16	0.6693231825938213
1111	17	1.1930526259864394
1111	18	1.874788444994337
1111	19	0.9727654337828824
1111	20	0.7429544033862427
1111	21	0.6810268630010015
1111	22	0.937256504529671
1111	23	0.8840815968468476
1111	24	0.7647577450894474
1111	25	0.3161456692593845
1112	1	0.13948734065753499
1112	2	0.6396278246735285
1112	3	0.3762659048131205
1112	4	0.8423440013635073
1112	5	0.5593606302285394
1112	6	0.020275109374885858
1112	7	-0.04579636083332872
1112	8	-0.06405251451786143
1112	9	0.12308426155165364
1112	10	0.4578004526245536
1112	11	0.0378765503372378
1112	12	0.04464943067144844
1112	13	-0.15660353478335765
1112	14	0.23525132189180908
1112	15	0.10599533875574707
1112	16	0.3784666521677593
1112	17	0.6195001705197427
1112	18	0.7456001644269534
1112	19	0.17488083158923473
1112	20	0.08618465970613443
1112	21	0.10681187185866747
1112	22	0.10568645499764528
1112	23	1.7682030778162101
1112	24	0.7201591748002585
1112	25	0.6820072271895654
1113	1	0.2808783435825646
1113	2	0.6327319474609929
1113	3	0.3173870591754131
1113	4	0.4630571985594827
1113	5	0.6530191890043042
1113	6	0.16429381499339257
1113	7	0.1037483265799537
1113	8	0.005271180933561936
1113	9	0.20404301548087744
1113	10	0.4156767826477079
1113	11	0.08488951962672786
1113	12	0.1084035646040391
1113	13	0.04714177402766495
1113	14	0.669658378349844
1113	15	0.27891249633875503
1113	16	0.6172594301249745
1113	17	1.0377319618829475
1113	18	1.6403903403380085
1113	19	0.6276295840172779
1113	20	0.3691966646008922
1113	21	0.42946040911751737
1113	22	0.5268900378150434
1113	23	0.49926368456606696
1113	24	0.4101993427505093
1113	25	0.7728674945816465
1114	1	-0.28674340170559276
1114	2	-2.7249911160535483
1114	3	-0.8481037350287473
1114	4	0.3147360306762721
1114	5	0.39404544458770374
1114	6	0.14318476171961692
1114	7	0.06669853902140233
1114	8	0.08020962738742554
1114	9	0.22257226703333544
1114	10	0.3669786673846076
1114	11	0.24995760983262727
1114	12	0.3151624708029743
1114	13	0.3050435062425052
1114	14	1.0288870524973532
1114	15	0.35469199301595467
1114	16	0.6882715229455414
1114	17	1.0447959122043358
1114	18	1.2721410757122342
1114	19	0.6734907889415638
1114	20	0.5479089426268189
1114	21	0.40073614721534767
1114	22	0.4836304234779689
1114	23	2.120466429963166
1114	24	1.1056934052845548
1114	25	1.0792821366995256
1115	1	-0.1902946470422897
1115	2	0.09979458756115633
1115	3	-0.12905825819851202
1115	4	-0.17125816609891586
1115	5	-0.10263459741098302
1115	6	-0.42069906436880444
1115	7	-0.5814465121408219
1115	8	-0.587341340202471
1115	9	-0.5779496444538097
1115	10	-0.32615134967998927
1115	11	-0.3168146146457005
1115	12	-0.5405989867785692
1115	13	-0.6692902486206975
1115	14	0.24461610628554808
1115	15	-0.36814110851102555
1115	16	-0.05355154813449303
1115	17	0.48733737959118173
1115	18	0.9006874710006372
1115	19	0.18202136107893097
1115	20	-0.33514980713383125
1115	21	-0.2916822488810453
1115	22	-0.015573831939676097
1115	23	1.6700207094732349
1115	24	0.48603288762343766
1115	25	0.14081963356961325
1116	1	0.35147063658796185
1116	2	0.3921911849735693
1116	3	0.4044124548928387
1116	4	1.052438216441189
1116	5	0.39174180700097594
1116	6	0.3664576861465676
1116	7	0.31772274609175355
1116	8	0.2482213544127987
1116	9	0.4119544897464138
1116	10	0.736940614793717
1116	11	0.32372341069408606
1116	12	0.4292198408568879
1116	13	0.24719433104998245
1116	14	0.8954523794584688
1116	15	0.39464018526687994
1116	16	0.873111531379358
1116	17	1.10907647727025
1116	18	1.6504689705706141
1116	19	0.7022605557062889
1116	20	0.3869519049055583
1116	21	0.3716760160895305
1116	22	0.6710925465916517
1116	23	2.0208465542616025
1116	24	0.9294992667891862
1116	25	1.1750646244755103
1201	1	0.9269254318065734
1201	2	1.2843499287477051
1201	3	1.0456519968469777
1201	4	1.686247706140236
1201	5	1.477459020171473
1201	6	1.0869671982419504
1201	7	0.9702786938602301
1201	8	1.0935292314850287
1201	9	1.1603989418477312
1201	10	1.444479112462048
1201	11	1.1282681439736777
1201	12	1.1971130166844404
1201	13	1.1295014981493097
1201	14	2.0787060864146625
1201	15	1.4231482719760606
1201	16	1.8346297308953439
1201	17	2.226247393951347
1201	18	2.714168319263422
1201	19	1.8671655888973007
1201	20	1.4986965612940892
1201	21	1.4308327169914605
1201	22	1.7175113544356293
1201	23	3.311776401141394
1201	24	2.2448325052176727
1201	25	2.3999271862354448
1202	1	0.985064613809854
1202	2	1.2995093724046747
1202	3	0.9902448681247478
1202	4	1.7109493393026582
1202	5	1.4244294183573771
1202	6	0.9996867287540923
1202	7	0.9049352888419762
1202	8	0.9822601918147029
1202	9	0.9626400991189215
1202	10	1.5368668860602739
1202	11	1.0833782335709827
1202	12	1.1139757693481585
1202	13	1.0364573467722948
1202	14	1.830777805875158
1202	15	1.2928932666077628
1202	16	1.6971766804403146
1202	17	2.083033046070689
1202	18	2.576794606413088
1202	19	1.7852397574186085
1202	20	1.4054844136229647
1202	21	1.4297918986609162
1202	22	1.8163857359112114
1202	23	3.3805553845665557
1202	24	2.1429067691175234
1202	25	2.286048351232303
1203	1	0.9199103792403207
1203	2	1.224306928767863
1203	3	0.9640122934464799
1203	4	1.5940786689563637
1203	5	1.3832810870611922
1203	6	0.9206043249031808
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2211	15	-0.23180897157753577
2211	16	-0.38277291866688756
2211	17	0.12887612067957832
2211	18	-5.427957624833127
2211	19	-1.732141997569066
2211	20	-1.021168607173891
2211	21	-0.6549399251533359
2211	22	-0.43248545078650125
2211	23	-4.976467173485641
2211	24	-2.0824391505146522
2211	25	-1.3846687854870012
2212	1	0.11496588135382879
2212	2	0.39673249686123313
2212	3	0.22884937947575423
2212	4	0.6677811249398502
2212	5	0.5966333269037634
2212	6	0.11164295884469055
2212	7	-0.004142892172907864
2212	8	-0.006752443276674569
2212	9	-0.1768021452186872
2212	10	-3.8635412376800993
2212	11	-1.126346630908941
2212	12	-0.3077249950516716
2212	13	-0.17536623805914076
2212	14	0.6092197738264531
2212	15	0.11846656647885112
2212	16	0.2574251658634594
2212	17	0.833243361937889
2212	18	1.4329990758017956
2212	19	0.6748413778090594
2212	20	0.34897965150685195
2212	21	0.21438578225184557
2212	22	0.41185844730134846
2212	23	1.735416051565423
2212	24	0.8320764602305957
2212	25	0.7497496482305834
2213	1	-1.4112526803468235
2213	2	-3.6286173240122466
2213	3	-1.6940724338498008
2213	4	-3.320122593416741
2213	5	-1.271853623752122
2213	6	-0.9257685034293459
2213	7	-0.9822484355233456
2213	8	-0.9401871145134209
2213	9	-0.8764644028509352
2213	10	-0.8698977222424062
2213	11	-0.9441530831099598
2213	12	-0.8700719626630402
2213	13	-1.1807742180771896
2213	14	-6.373827017848953
2213	15	-2.8469392257343884
2213	16	-2.083827261232507
2213	17	-0.8955700274275529
2213	18	0.015531277553861855
2213	19	-0.7542381944751
2213	20	-0.8757311480446504
2213	21	-1.1801907407793593
2213	22	-0.8763292298880501
2213	23	-5.745454330115358
2213	24	-3.140584183895605
2213	25	-2.4482635515251125
2214	1	-0.22769219842081512
2214	2	-0.153120642869208
2214	3	-0.2591210152431067
2214	4	0.09777059327287674
2214	5	-0.32489090412966704
2214	6	-0.4615851064079166
2214	7	-0.5893205730561064
2214	8	-0.6224080942083958
2214	9	-0.5051823456847799
2214	10	-0.015117981281385084
2214	11	-0.425760556396483
2214	12	-0.3982530542913878
2214	13	-0.46795444077004333
2214	14	0.08645706977540613
2214	15	-0.2892757575211391
2214	16	0.20009946221873776
2214	17	0.4360382622787995
2214	18	0.9032343619879981
2214	19	-0.0029366226683293917
2214	20	-0.10016469610375367
2214	21	-0.32218980432374167
2214	22	0.11597709436478709
2214	23	1.5192202427315777
2214	24	0.48424009828379866
2214	25	0.2841079362698551
2215	1	-2.4781951827947672
2215	2	-4.501498398505028
2215	3	-2.565974416854573
2215	4	-4.076896718491206
2215	5	-4.9279146785244095
2215	6	-2.360980447335514
2215	7	-1.8292057968122997
2215	8	-2.1553469840021258
2215	9	-1.8689160944991698
2215	10	-3.2358766254271245
2215	11	-2.214781365578581
2215	12	-2.0511309632176804
2215	13	-2.047008614582282
2215	14	-1.3743903870397958
2215	15	-2.017034267537717
2215	16	-2.7308611421289655
2215	17	-7.08923550493045
2215	18	-3.222674003839721
2215	19	-2.3833185554696144
2215	20	-2.2234341903409884
2215	21	-2.0567547707435523
2215	22	-1.925982580659408
2215	23	-6.497032140456291
2215	24	-3.733064121036545
2215	25	-3.4835827427111496
2216	1	-0.9868444685219906
2216	2	-0.5580848835998715
2216	3	-0.7857567389591225
2216	4	-2.971563800879032
2216	5	-1.1962746003308844
2216	6	-1.080543480840742
2216	7	-0.9429926457062834
2216	8	-0.8985663411695626
2216	9	-0.8677590075315109
2216	10	-0.5365811304817569
2216	11	-1.0107947764672005
2216	12	-1.6555843006737305
2216	13	-1.0397515527131418
2216	14	-7.026553711118591
2216	15	-3.3161109266920796
2216	16	-1.5544460514508671
2216	17	-0.7217285122762931
2216	18	0.05884517453311844
2216	19	-0.4369190931471465
2216	20	-0.9252185138582973
2216	21	-1.0803643581187359
2216	22	-0.5970208960054677
2216	23	0.3650705849322833
2216	24	-0.3867490427783338
2216	25	-0.32343543769687955
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	96552.0
3	4.0
4	759.0
5	4980.0
6	6160.0
7	5416.0
8	4902.0
9	4833.0
10	4468.0
11	4405.0
12	4309.0
13	4196.0
14	4332.0
15	4408.0
16	4552.0
17	4890.0
18	5505.0
19	6435.0
20	7841.0
21	9734.0
22	12156.0
23	14890.0
24	17544.0
25	20529.0
26	23679.0
27	27027.0
28	29856.0
29	36368.0
30	43279.0
31	48891.0
32	63229.0
33	83240.0
34	109079.0
35	143128.0
36	204234.0
37	286890.0
38	719456.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33504427446691	17.482713439262234	11.74481209923974	25.43743018703112
2	13.732607555773146	19.49537408541502	41.43923351091515	25.332784847896683
3	20.24372982033338	24.0693214997451	28.201035825184224	27.4859128547373
4	10.956004317694243	16.018964255439116	38.01690195783103	35.0081294690356
5	12.370275498465054	37.914723746010566	36.143851841910504	13.571148913613873
6	33.658689629207736	34.943430286999586	17.81603259033295	13.581847493459732
7	28.986857373444057	30.792544595536487	21.004164631332625	19.21643339968683
8	26.52496360399603	36.12786099622717	19.806618473337274	17.540556926439525
9	27.112486362802553	15.059569209380461	19.160914102551725	38.66703032526526
10	14.450800357441656	29.547110221315474	34.16836158573941	21.83372783550346
11	34.90343270988881	22.85158776589069	22.310586972345632	19.934392551874872
12	23.740456099263323	25.16727468960903	30.70237510269136	20.389894108436284
13	29.510541220058318	20.83000493769002	24.696510650785612	24.96294319146605
14	21.03812779029305	21.902832034314	27.70398674319674	29.35505343219621
15	25.728842357279735	28.340163439863687	22.42157444816118	23.509419754695397
16	22.162926121005235	27.64365896418035	28.16221559753479	22.031199317279622
17	23.010832102412603	27.670084281866362	27.740946698718876	21.57813691700215
18	22.548292739436416	26.632975180283243	29.380858601948123	21.43787347833222
19	23.715580452637052	27.130802848810575	27.511789987839503	21.641826710712873
20	23.66566088118779	27.039635928504435	27.463349811095433	21.83135337921234
21	22.951550888100968	26.637284551942862	27.642037020499092	22.769127539457074
22	22.947734713917296	27.200184996190973	26.124918774506444	23.72716151538529
23	21.03469449228797	26.965419431078296	28.799971808794112	23.199914267839628
24	22.75681907496758	28.299704246852976	26.64810203338737	22.29537464479207
25	22.98992124494204	26.220983390667374	26.541960698744766	24.247134665645824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	195.0
1	195.0
2	341.0
3	487.0
4	487.0
5	487.0
6	978.0
7	1469.0
8	1469.0
9	1469.0
10	2201.5
11	2934.0
12	2934.0
13	2934.0
14	5058.0
15	7182.0
16	7182.0
17	7182.0
18	12953.0
19	18724.0
20	18724.0
21	18724.0
22	30145.5
23	41567.0
24	41567.0
25	41567.0
26	59725.5
27	77884.0
28	77884.0
29	77884.0
30	101518.0
31	125152.0
32	125152.0
33	125152.0
34	146438.5
35	167725.0
36	167725.0
37	167725.0
38	188381.5
39	209038.0
40	209038.0
41	209038.0
42	230573.0
43	252108.0
44	252108.0
45	252108.0
46	291765.0
47	331422.0
48	331422.0
49	331422.0
50	311017.5
51	290613.0
52	290613.0
53	290613.0
54	251291.5
55	211970.0
56	211970.0
57	211970.0
58	179764.5
59	147559.0
60	147559.0
61	147559.0
62	121110.0
63	94661.0
64	94661.0
65	94661.0
66	73028.0
67	51395.0
68	51395.0
69	51395.0
70	37705.0
71	24015.0
72	24015.0
73	24015.0
74	17016.0
75	10017.0
76	10017.0
77	10017.0
78	6919.5
79	3822.0
80	3822.0
81	3822.0
82	2673.0
83	1524.0
84	1524.0
85	1524.0
86	1015.5
87	507.0
88	507.0
89	507.0
90	324.5
91	142.0
92	142.0
93	142.0
94	86.5
95	31.0
96	31.0
97	31.0
98	22.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11292586079426452
2	0.04816239703960513
3	0.036869810960178676
4	0.032816062111153795
5	0.036049409407399834
6	0.018145351990873276
7	0.02046177990460178
8	0.02412945743467191
9	0.02972749155951579
10	0.03711110553452539
11	0.021233922542511278
12	0.019979190755908337
13	0.016263254310968867
14	0.022440395414244873
15	0.02152347603172734
16	0.021426958201988654
17	0.025866778369968285
18	0.020075708585647025
19	0.03353994583419395
20	0.02292298456293831
21	0.029003607836475633
22	0.03677329313043999
23	0.028376241943174164
24	0.040247935001032735
25	0.032333472962460356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2072156.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.6385221577573
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.76681239323376	59.878023554818995
2	10.371292331123607	14.652255262733066
3	2.618225173402364	5.548426707761425
4	0.946278544743859	2.6737487180119768
5	0.4085711201617549	1.4430430062282922
6	0.22964697754900718	0.9733153867234522
7	0.1466551023446305	0.7251649787563542
8	0.095103471403198	0.5374374937595552
9	0.0728159673210263	0.46292510889403626
>10	0.3076388450117993	3.800631625465487
>50	0.019987588544174234	0.9616809500680437
>100	0.012870041445104204	1.8027742378159146
>500	0.0014601918310435487	0.8043898343587425
>1k	0.002225054218733011	2.8198095127639484
>5k	2.781317773416264E-4	1.1683471325573254
>10k+	1.390658886708132E-4	1.7480264892833335
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	19874	0.959097674113339	No Hit
CCCATGTACTCTGCGTTGATACCAC	15715	0.758388847171738	No Hit
GAGTACATGGGAAGCAGTGGTATCA	8100	0.39089721044168485	No Hit
GTATCAACGCAGAGTACATGGGAAG	5331	0.25726827516847184	No Hit
GCGTTGATACCACTGCTTCCCATGT	5224	0.25210457127745206	No Hit
CATGTACTCTGCGTTGATACCACTG	5132	0.24766475110947245	No Hit
GCTTCCCATGTACTCTGCGTTGATA	4257	0.2054382005987966	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4241	0.2046660579608871	No Hit
TATCAACGCAGAGTACATGGGAAGC	4083	0.19704114941153078	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3576	0.17257387957277348	No Hit
GGTATCAACGCAGAGTACATGGGAA	2696	0.13010603448775093	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2267	0.10940296000880242	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.825891486934381E-5	0.0
10	0.0	0.0	0.0	4.825891486934381E-5	0.0
11	0.0	0.0	0.0	4.825891486934381E-5	0.0
12	0.0	0.0	0.0	9.651782973868763E-5	0.0
13	0.0	0.0	0.0	9.651782973868763E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	190	0.0	14.000498	18
TAGGACC	530	0.0	13.086319	4
GCGTTTA	270	0.0	13.016468	13
GTATAAT	325	0.0	12.292198	1
GATATAC	505	0.0	12.242937	1
ATACGAC	70	1.0911216E-4	12.214426	19
GTAGGAC	1085	0.0	12.083963	3
CGTTTAT	300	0.0	11.71482	14
CGGTTTC	155	2.5465852E-11	11.643327	13
GTATTAG	180	0.0	11.625558	1
TGCGTTT	355	0.0	11.505229	12
GTCCTAG	325	0.0	11.414184	1
CCAACGA	150	1.7644197E-10	11.40013	19
CCGGTTT	150	1.7644197E-10	11.398204	12
TGTAGGA	1230	0.0	11.277369	2
TAGGACA	520	0.0	11.145434	4
AGGACCT	890	0.0	11.101251	5
ACTGTTC	685	0.0	10.816075	8
CGCAGAT	185	5.456968E-12	10.782346	8
TACACTG	1195	0.0	10.652842	5
>>END_MODULE