FastQCFastQC Report
Thu 26 May 2016
SRR1513059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2072156
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC224401.0829300496680752No Hit
CCCATGTACTCTGCGTTGATACCAC210401.015367568850994No Hit
GAGTACATGGGAAGCAGTGGTATCA85100.4106833655381159No Hit
CATGTACTCTGCGTTGATACCACTG61570.29713013885054984No Hit
GCGTTGATACCACTGCTTCCCATGT56620.2732419759902247No Hit
GTATCAACGCAGAGTACATGGGAAG53860.2599225154862858No Hit
ACGCAGAGTACATGGGAAGCAGTGG53510.25823345346585874No Hit
GCTTCCCATGTACTCTGCGTTGATA52800.25480707051013535No Hit
TATCAACGCAGAGTACATGGGAAGC48070.23198060377693572No Hit
ACTCTGCGTTGATACCACTGCTTCC43750.21113275255337918No Hit
GTATCAACGCAGAGTACTTTTTTTT33640.16234298962047258No Hit
GGTATCAACGCAGAGTACATGGGAA32610.15737232138893018No Hit
TATCAACGCAGAGTACTTTTTTTTT25330.12223983136404788No Hit
CAGTGGTATCAACGCAGAGTACATG24140.11649702049459597No Hit
ATACCACTGCTTCCCATGTACTCTG22140.10684523752072721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGT1800.012.6923896
TCTACAC3350.011.9346343
GGACGTG7200.011.7669026
TGGCGAG9200.011.76060818
AGGACGT7450.011.7553655
GACGTGA3900.011.6764587
GCTTAGG1156.7868314E-811.6002221
GTCCTAC10450.011.4892131
TTAGGAC4850.011.3838933
ATGGCGA9950.011.25812417
GTCTAAT1451.1586963E-911.1716411
TAGGACC5900.011.132734
TTTGCGC600.00590873511.07525917
TAGGACG7750.011.054664
AAAGGCG1900.011.0223375
TATGGCG10000.011.01197216
GTCTTAT1752.0008883E-1110.8900041
CCTACAC3150.010.879193
GTAGGAC10500.010.87918953
TCCTACA12550.010.7781412
Produced by FastQC (version 0.11.5)