FastQCFastQC Report
Thu 26 May 2016
SRR1513058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934152
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC126210.6525340304174646No Hit
CCCATGTACTCTGCGTTGATACCAC98410.5088017901385207No Hit
GAGTACATGGGAAGCAGTGGTATCA52290.27035103756064677No Hit
GTATCAACGCAGAGTACATGGGAAG35370.18287083952036862No Hit
GCGTTGATACCACTGCTTCCCATGT33560.17351273322882588No Hit
CATGTACTCTGCGTTGATACCACTG30530.1578469530833151No Hit
TATCAACGCAGAGTACATGGGAAGC27350.14140563926723443No Hit
ACGCAGAGTACATGGGAAGCAGTGG27090.14006138090491338No Hit
GCTTCCCATGTACTCTGCGTTGATA26500.13701094846733866No Hit
GTATCAACGCAGAGTACTTTTTTTT24000.12408538729117462No Hit
ACTCTGCGTTGATACCACTGCTTCC22160.1145721742655179No Hit
GTCCTACAGTGGACATTTCTAAATT19480.10071597268467007No Hit
GGTATCAACGCAGAGTACATGGGAA19460.10061256819526078No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC602.5682391E-514.25038919
TAGGACC5700.013.5021124
GCGTTTA2050.013.4366113
CGTTTAT2050.013.4366114
GATACGA500.001499470213.30036318
CTAGGAC2900.012.4489363
TACCCCG550.00306650312.0921765
TAGGACA5300.011.8320424
GTAGGAC12800.011.8014083
GTCCTAC13400.011.7808621
GTCCTAT2050.011.5973811
TCCTACA15500.011.2786022
TGTAGGA14500.011.2701472
ATACCGA600.00587760711.0830636
TCCAACG1553.1286618E-1011.03255818
ACAGCGT951.3620995E-510.9988788
TGCGTTT2700.010.90544812
GGCGAGG4100.010.8905419
ACCGTGC1158.830448E-710.7380338
CGGGAGT803.7661183E-410.6891744
Produced by FastQC (version 0.11.5)