##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513058_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1934152
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.279675020370686	31.0	30.0	34.0	25.0	34.0
2	29.046873772071688	31.0	30.0	34.0	16.0	34.0
3	29.366700755680007	31.0	30.0	34.0	23.0	34.0
4	32.20031879604085	35.0	33.0	37.0	19.0	37.0
5	32.45832902481294	35.0	33.0	37.0	22.0	37.0
6	32.87561629075688	37.0	33.0	37.0	26.0	37.0
7	32.88383849873226	37.0	35.0	37.0	26.0	37.0
8	32.8598398678077	37.0	35.0	37.0	26.0	37.0
9	34.22645738287373	39.0	35.0	39.0	25.0	39.0
10	33.72779595398914	39.0	34.0	39.0	19.0	39.0
11	34.10953999478841	39.0	35.0	39.0	25.0	39.0
12	33.973350594989434	39.0	35.0	39.0	24.0	39.0
13	34.044388445168735	39.0	35.0	39.0	25.0	39.0
14	34.392496556630505	40.0	34.0	41.0	17.0	41.0
15	34.907501582088685	40.0	34.0	41.0	23.0	41.0
16	34.479802518106126	39.0	34.0	41.0	17.0	41.0
17	34.01584311884485	39.0	33.0	41.0	11.0	41.0
18	33.41248050825374	39.0	32.0	41.0	10.0	41.0
19	34.31337971369365	39.0	34.0	41.0	18.0	41.0
20	34.59249014555216	39.0	34.0	41.0	18.0	41.0
21	34.621716390438806	40.0	34.0	41.0	18.0	41.0
22	34.21278110510446	40.0	34.0	41.0	10.0	41.0
23	32.507629183228616	39.0	31.0	41.0	2.0	41.0
24	33.62138808118493	39.0	33.0	41.0	2.0	41.0
25	33.356304985337246	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7032800867226001
1101	2	1.1164837996853798
1101	3	0.8391065491985721
1101	4	0.8158983542824814
1101	5	1.0508273536730712
1101	6	0.6958794243797044
1101	7	0.7609335457429722
1101	8	0.746878035036417
1101	9	0.8382937598829017
1101	10	1.3080170674893523
1101	11	0.936776763812901
1101	12	1.016189214234494
1101	13	0.9500265063022084
1101	14	0.09883708602514929
1101	15	0.5834116146832358
1101	16	1.2792860480038968
1101	17	1.8475812603178525
1101	18	-4.44721592517358
1101	19	-0.7200696466044505
1101	20	0.270268803295707
1101	21	0.8532902912417839
1101	22	1.283266691353056
1101	23	3.0461794705217144
1101	24	2.021043426415538
1101	25	2.2133347263320786
1102	1	0.8687336255297424
1102	2	1.0161621140278108
1102	3	0.8723466646274751
1102	4	1.4717975729828083
1102	5	1.1938392780296994
1102	6	0.9155912583146772
1102	7	0.7389877492906081
1102	8	0.7694724126457544
1102	9	0.8574231511614485
1102	10	1.1687204667787015
1102	11	0.8801170848097968
1102	12	1.006739973988374
1102	13	0.8061470108268267
1102	14	1.6992241154616394
1102	15	1.1287628463251664
1102	16	1.4095179654376153
1102	17	0.8630929021067928
1102	18	1.3406631570465493
1102	19	1.2127494429624477
1102	20	1.0550939468754166
1102	21	1.0769386463446082
1102	22	1.4417924650587182
1102	23	3.0453026276505426
1102	24	1.900401591562229
1102	25	2.3032994230309853
1103	1	0.7851222366730894
1103	2	1.1009081926079745
1103	3	0.9271977756592271
1103	4	1.3395476332436687
1103	5	1.1437546896337096
1103	6	0.7284936669636721
1103	7	0.6739196321970553
1103	8	0.6533228362572956
1103	9	0.7207808641054854
1103	10	1.0185755059259378
1103	11	0.8053415007258096
1103	12	0.7453491086423867
1103	13	0.5271728069397739
1103	14	1.3502265660700488
1103	15	0.8976742673209444
1103	16	1.126815064881427
1103	17	1.4919195784747146
1103	18	1.752580300592392
1103	19	1.0930060872805143
1103	20	1.0364114055632498
1103	21	0.8979401149241468
1103	22	1.2381192307019973
1103	23	2.6780977050076373
1103	24	1.617048878037906
1103	25	1.8785800824187362
1104	1	0.36654466481984826
1104	2	0.8317328163734494
1104	3	0.6575527631521894
1104	4	1.1755414643582611
1104	5	0.8464693974911839
1104	6	0.5337391003179164
1104	7	0.44863494632232204
1104	8	0.3996284195632498
1104	9	0.476007766464285
1104	10	0.859488292976593
1104	11	0.47468581325906456
1104	12	0.5101079125247168
1104	13	0.4387159022908733
1104	14	1.2296593737869088
1104	15	0.7185720304361851
1104	16	1.0525404012328252
1104	17	1.5138255383747463
1104	18	1.9973881369354913
1104	19	1.169297365764315
1104	20	0.6667783413995423
1104	21	0.7263073627449543
1104	22	1.1325330482972547
1104	23	2.448412046006716
1104	24	1.5646742001176719
1104	25	1.6013008529541182
1105	1	0.4857556078132852
1105	2	0.8740119439093768
1105	3	0.5112293539008732
1105	4	0.629241984987118
1105	5	0.7756436604651711
1105	6	0.4222288279549673
1105	7	0.28656389437314544
1105	8	0.35457833067297884
1105	9	0.5103585815868286
1105	10	0.9942136283193292
1105	11	0.3933364275509632
1105	12	0.4152831230163869
1105	13	0.41859500800838134
1105	14	1.1463752528679905
1105	15	0.5138368394139334
1105	16	1.0029908130445122
1105	17	1.5040989658483994
1105	18	1.2928181236706848
1105	19	0.9222019979336835
1105	20	0.8142444684122196
1105	21	0.6445774743466117
1105	22	1.2628978887929492
1105	23	2.581495391272
1105	24	1.5391371909523954
1105	25	1.5650846948743862
1106	1	-0.6214986852953999
1106	2	-0.1390597689019195
1106	3	-0.33902297261189673
1106	4	-2.6867401784604787
1106	5	-0.6018599185123854
1106	6	-0.5577735360201501
1106	7	-0.6604501027387855
1106	8	-0.5717016619884348
1106	9	-0.4842340709034829
1106	10	-0.4326742202701368
1106	11	-0.4550457829141976
1106	12	-0.37925327865333003
1106	13	-0.41295408436596404
1106	14	0.25133029095626114
1106	15	-0.3723228066140791
1106	16	-1.348792258296136
1106	17	-5.621127322558962
1106	18	-7.064368038714761
1106	19	-3.0686202997868257
1106	20	-1.6926596813463703
1106	21	-1.364794279075717
1106	22	-1.770535224080966
1106	23	-5.3022977694183915
1106	24	-2.665467911152014
1106	25	-1.3482063131907438
1107	1	0.4444088655292546
1107	2	0.8053529585518646
1107	3	0.5830291163281665
1107	4	0.9667794309114299
1107	5	0.8512776140793576
1107	6	0.5488490249957465
1107	7	0.4849325776069904
1107	8	0.45807456192363105
1107	9	0.3993808676817139
1107	10	0.7816286091451445
1107	11	0.4540859460552795
1107	12	0.5601271737828739
1107	13	0.49001924742616865
1107	14	1.2586699903859184
1107	15	0.7116021651101292
1107	16	-5.509001093066551
1107	17	-0.9814494846452035
1107	18	0.7218785847065092
1107	19	0.6180085391653236
1107	20	0.5494221087854854
1107	21	0.5961473942856728
1107	22	0.5663062610170471
1107	23	2.4595287279339786
1107	24	1.5639218173032319
1107	25	1.3216848133914283
1108	1	0.14653271809127588
1108	2	0.4424962436990434
1108	3	0.11395896760855351
1108	4	0.6838917570792589
1108	5	0.39423735908389546
1108	6	0.22680490012786692
1108	7	0.1106784643071137
1108	8	0.07777845426627295
1108	9	0.16312307592246356
1108	10	0.6369203475916976
1108	11	0.1801780406965463
1108	12	0.27276209917339855
1108	13	0.11478310198554453
1108	14	0.7126601201306713
1108	15	0.4779284494500402
1108	16	0.6768027168852626
1108	17	0.7344269790404141
1108	18	0.4920539356997722
1108	19	0.39480428480235474
1108	20	0.27811492413847816
1108	21	0.39810563295277035
1108	22	0.7086052149031445
1108	23	2.3557412463470797
1108	24	1.2303304924752965
1108	25	1.4134548847135946
1109	1	0.002256244358406434
1109	2	0.27717287341386054
1109	3	0.1414486523637528
1109	4	0.04880695469549323
1109	5	-0.13844503974436861
1109	6	-0.19236415469236334
1109	7	-0.11707010952042651
1109	8	-0.04462214884116378
1109	9	0.0013850291696897443
1109	10	0.4891212673561398
1109	11	0.15409948911008087
1109	12	0.14432295538458106
1109	13	0.0722624490969963
1109	14	-1.2863144351010547
1109	15	-0.38204468888879006
1109	16	-0.6773917084667502
1109	17	0.4322288111456487
1109	18	1.2507070725538796
1109	19	0.5680575195291553
1109	20	0.27357988558456725
1109	21	0.23300027900899778
1109	22	-0.10040095056486109
1109	23	-4.6102334850896405
1109	24	-1.7696961729285015
1109	25	-0.5640051694449042
1110	1	0.26532265587490045
1110	2	0.7377863281570036
1110	3	0.46499989342283854
1110	4	1.1138383306638886
1110	5	0.6774478618482362
1110	6	0.3007011521313814
1110	7	0.21517400643624995
1110	8	0.3336532496099025
1110	9	0.3929424407564426
1110	10	0.6777061020594957
1110	11	0.39087514591002304
1110	12	0.37793935673499846
1110	13	0.20470381385057834
1110	14	1.0136684038183645
1110	15	0.554789580877177
1110	16	0.9544310905262137
1110	17	1.2669776572805986
1110	18	1.864475191950234
1110	19	1.0630893037986269
1110	20	0.6547282105864198
1110	21	0.601905789643375
1110	22	0.812367805325195
1110	23	2.192726739131899
1110	24	1.1121389061855353
1110	25	1.4245828156017382
1111	1	0.6908971698843267
1111	2	1.056529787029035
1111	3	0.7833975221102989
1111	4	1.2950126493226506
1111	5	1.0250882835710016
1111	6	0.6350038704723175
1111	7	0.4684076426154533
1111	8	0.27423759932727165
1111	9	0.40686634076235606
1111	10	0.8005212671882447
1111	11	0.607986054931068
1111	12	0.7129102875801152
1111	13	0.5622084528092373
1111	14	-0.05603311209127071
1111	15	0.3633435479460445
1111	16	0.6395181037970872
1111	17	1.3573349862155126
1111	18	1.9713428133616233
1111	19	0.9703890476694355
1111	20	0.781762648579317
1111	21	0.6283551879112466
1111	22	0.977138121609002
1111	23	1.1441734414117732
1111	24	1.0641426760237707
1111	25	0.6106040248670155
1112	1	0.5204752275172488
1112	2	0.86108509370343
1112	3	0.6527970033671338
1112	4	1.2553441419773392
1112	5	0.9527471619860393
1112	6	0.4435030318304314
1112	7	0.415122020581876
1112	8	0.4219355433226184
1112	9	0.47867774330833157
1112	10	0.8476420721139561
1112	11	0.4767152620468451
1112	12	0.48744040302924674
1112	13	0.5085420852262104
1112	14	1.0423708042704547
1112	15	0.6511414682846564
1112	16	1.1163940721780534
1112	17	1.3398027885475159
1112	18	1.4450837316265392
1112	19	0.8346991341763541
1112	20	0.7216473699520023
1112	21	0.8027677656214891
1112	22	0.7048284357747008
1112	23	2.359801698208827
1112	24	1.3687619198181409
1112	25	1.355305981982255
1113	1	0.30543494771792723
1113	2	0.698990757400427
1113	3	0.46181264077963036
1113	4	0.5879054680384499
1113	5	0.7891653254831041
1113	6	0.4709295498537074
1113	7	0.22235958875896245
1113	8	0.1704365803075234
1113	9	0.3182777710323492
1113	10	0.6008620568657506
1113	11	0.23530784160677598
1113	12	0.21204386959781374
1113	13	0.12376097168036182
1113	14	0.9182887625031526
1113	15	0.49135089125186937
1113	16	0.9434847030594042
1113	17	1.350786298088309
1113	18	1.7392392953571303
1113	19	0.863788026975044
1113	20	0.5509393944984211
1113	21	0.4395212831062807
1113	22	0.727107350059498
1113	23	0.3831325257174498
1113	24	0.47172252505477985
1113	25	0.8817920160081556
1114	1	-0.5228431462447993
1114	2	-3.196736760255444
1114	3	-1.2526898860429512
1114	4	-0.22866156051384934
1114	5	-0.018769056840298504
1114	6	-0.146361249961366
1114	7	-0.26304701445815226
1114	8	-0.16189080662314836
1114	9	-0.16468012588604353
1114	10	0.034592348446729204
1114	11	-0.1803648352309466
1114	12	-0.277866376388765
1114	13	-0.28832154462043036
1114	14	0.46428968714485563
1114	15	-0.23739430619101398
1114	16	0.13148349145993876
1114	17	0.5008381099077823
1114	18	0.7988643372051385
1114	19	0.15238145564212147
1114	20	-0.002221126489352798
1114	21	-0.2758070444081824
1114	22	0.023284434887408167
1114	23	1.6149497655025513
1114	24	0.5995813418322413
1114	25	0.5269639515383133
1115	1	0.38104360449369423
1115	2	0.7520147060368636
1115	3	0.4744322304248918
1115	4	0.6350480679233215
1115	5	0.6894551199101855
1115	6	0.2475519666601187
1115	7	0.2165427775594111
1115	8	0.17479525920301597
1115	9	0.19788557008322982
1115	10	0.4007300671626126
1115	11	0.41753680210459265
1115	12	0.20848972415306832
1115	13	0.18743299184106377
1115	14	1.1425632094839955
1115	15	0.54712840740968
1115	16	0.9118941959087508
1115	17	1.500307419043132
1115	18	1.9360256375456402
1115	19	1.0240641227862426
1115	20	0.65714790700153
1115	21	0.635663811167106
1115	22	0.7563462961820946
1115	23	2.569789282747095
1115	24	1.323362767757608
1115	25	0.960957004178006
1116	1	0.9415246698471549
1116	2	0.8324914827658993
1116	3	0.9178629327857308
1116	4	1.5775234422228763
1116	5	1.0643727056118095
1116	6	0.8259978086045905
1116	7	0.678934375799102
1116	8	0.7402985748765829
1116	9	0.82869699507728
1116	10	1.305801981725324
1116	11	0.8383543765917167
1116	12	1.0204915874864398
1116	13	0.770463348647219
1116	14	1.500195543740368
1116	15	1.0019598262665639
1116	16	1.4214670710986894
1116	17	1.7367439665032904
1116	18	2.2915846437185436
1116	19	1.4478932235676893
1116	20	1.200625069460294
1116	21	1.061412441087164
1116	22	1.4919215449038248
1116	23	2.781123821331775
1116	24	1.7992529346072814
1116	25	1.9423990911113833
1201	1	0.8300304169735924
1201	2	1.1066638965590059
1201	3	0.8350391779964568
1201	4	1.3302744000772861
1201	5	1.0893918626999337
1201	6	0.6871625595624451
1201	7	0.6013175175615544
1201	8	0.5524786515049342
1201	9	0.7138732402880095
1201	10	1.2053345487751344
1201	11	0.7861589742928601
1201	12	0.8941927369117266
1201	13	0.7705667969230845
1201	14	1.6036706395258093
1201	15	0.9723314737761442
1201	16	1.3444555768458173
1201	17	1.7087248094151661
1201	18	2.382944796975252
1201	19	1.5222993538358836
1201	20	1.2183568702265788
1201	21	1.1127381115852444
1201	22	1.3443468322601504
1201	23	3.033054855814534
1201	24	1.8932759231130305
1201	25	1.982491045134374
1202	1	0.7152205886329632
1202	2	1.092667823407428
1202	3	0.9460748562192762
1202	4	1.5184552594961076
1202	5	1.2312083105850462
1202	6	0.8228618751688614
1202	7	0.787507801416595
1202	8	0.7671418401058503
1202	9	0.9004071794779591
1202	10	1.3241526795952865
1202	11	0.9631430425078307
1202	12	0.9926233438903012
1202	13	0.8901908292354364
1202	14	1.818959431178449
1202	15	1.2751014726541854
1202	16	1.4384194498403176
1202	17	1.951497052206598
1202	18	2.535936494495729
1202	19	1.676034347665123
1202	20	1.4410513614192197
1202	21	1.342406236795611
1202	22	1.805982757313437
1202	23	3.2941922955093617
1202	24	2.165796959801014
1202	25	2.347473430414297
1203	1	0.9595006156490875
1203	2	1.3053239407091155
1203	3	0.9764100389236106
1203	4	1.5951441572516742
1203	5	1.397912834002362
1203	6	0.9871139944410317
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2211	15	-0.31290192403172057
2211	16	-0.6323335914327117
2211	17	-0.06685030341526499
2211	18	-5.814279574398057
2211	19	-2.09441244500357
2211	20	-1.194633458593536
2211	21	-0.8772915466997304
2211	22	-0.4716125487843428
2211	23	-5.229107620007515
2211	24	-2.523900092360858
2211	25	-1.6272600831699506
2212	1	0.43928296883397877
2212	2	0.6161679027054205
2212	3	0.4298659807623437
2212	4	0.9790980198775259
2212	5	0.7364635343056847
2212	6	0.3461611327157783
2212	7	0.22723761414809474
2212	8	0.24131313729134263
2212	9	0.012624090908140317
2212	10	-3.8781225909460844
2212	11	-0.9839899665478811
2212	12	-0.16283020239241353
2212	13	0.10054407713381863
2212	14	0.8056729444319615
2212	15	0.3662819731724767
2212	16	0.6046355790409663
2212	17	1.1334381229921604
2212	18	1.7701586044607112
2212	19	0.9416034916912253
2212	20	0.4899595483080148
2212	21	0.4020920793165601
2212	22	0.6307881669105697
2212	23	1.9344428483510256
2212	24	1.1181754950590275
2212	25	0.9511372969179277
2213	1	-1.5285846142046182
2213	2	-3.8198735719892944
2213	3	-1.8018302017212982
2213	4	-3.5332206223491482
2213	5	-1.4119132461503021
2213	6	-1.235448095726909
2213	7	-1.0763875475297944
2213	8	-0.9358923144725324
2213	9	-0.9605837119054002
2213	10	-0.7801504091069376
2213	11	-0.8760811526079166
2213	12	-0.7922546977234362
2213	13	-1.121106652371303
2213	14	-6.240237794999956
2213	15	-2.7504698608699556
2213	16	-1.8833641365334657
2213	17	-0.7128315848524167
2213	18	0.028777309829422393
2213	19	-0.7859076257068338
2213	20	-0.8073370363481942
2213	21	-1.0692743596979852
2213	22	-0.9509516033595915
2213	23	-5.5528628774750395
2213	24	-3.0576200401295033
2213	25	-2.0932538405455325
2214	1	0.1942231253069231
2214	2	0.25283316739233896
2214	3	0.15157042799645737
2214	4	0.5067275250772809
2214	5	0.29298561269993684
2214	6	-0.011772336270887251
2214	7	-0.10056529493844835
2214	8	-0.22949009849507007
2214	9	-0.019366343045319923
2214	10	0.3973736112751354
2214	11	-0.07910925487380638
2214	12	-0.019762992254939604
2214	13	0.009790755256418038
2214	14	0.5668659520258075
2214	15	0.2176491821094757
2214	16	0.5876196393458173
2214	17	0.8425711635818374
2214	18	1.370757296975249
2214	19	0.4801639371692161
2214	20	0.35388551605991125
2214	21	0.3025193615852473
2214	22	0.727154123926816
2214	23	2.1197058974812037
2214	24	1.0817915481130314
2214	25	0.9262644826343731
2215	1	-2.183363015093377
2215	2	-4.093641104514187
2215	3	-2.2865055740962674
2215	4	-3.575325943344051
2215	5	-4.721204831869716
2215	6	-2.1367515851037915
2215	7	-1.640085787868795
2215	8	-2.062460391208095
2215	9	-1.50711602779527
2215	10	-2.919817200527291
2215	11	-1.8804138954216683
2215	12	-1.5598712253119231
2215	13	-1.6388156885888279
2215	14	-0.870451060409799
2215	15	-1.5490192452622722
2215	16	-2.3060058955731577
2215	17	-6.851406763883823
2215	18	-3.156023865827926
2215	19	-2.221890386451733
2215	20	-1.789162339904351
2215	21	-1.8135387718661349
2215	22	-1.4787003881734222
2215	23	-6.001249930620322
2215	24	-3.4255392732810463
2215	25	-3.0796072592227866
2216	1	-0.530292036821141
2216	2	0.07544735441717876
2216	3	-0.22954163207039358
2216	4	-2.782787862342431
2216	5	-0.823921808816646
2216	6	-0.5351334163190558
2216	7	-0.61837549593956
2216	8	-0.4261333553111655
2216	9	-0.3484493445854966
2216	10	0.0640130125318521
2216	11	-0.5194319288399214
2216	12	-1.128182934867013
2216	13	-0.5806722907686179
2216	14	-6.384902305814343
2216	15	-2.756999106902846
2216	16	-1.0677216416625086
2216	17	-0.2085832257139444
2216	18	0.6200394358532151
2216	19	0.1467481675729445
2216	20	-0.333627924635131
2216	21	-0.48444946214656426
2216	22	0.04719294577436983
2216	23	1.122462904051524
2216	24	0.34501796007725005
2216	25	0.4883114672080353
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	92847.0
3	0.0
4	751.0
5	4669.0
6	5904.0
7	5157.0
8	4763.0
9	4377.0
10	4231.0
11	4236.0
12	4087.0
13	3999.0
14	3854.0
15	4043.0
16	4245.0
17	4464.0
18	5061.0
19	6095.0
20	7346.0
21	9231.0
22	11293.0
23	13837.0
24	16271.0
25	19157.0
26	22044.0
27	25173.0
28	28388.0
29	34328.0
30	40375.0
31	45533.0
32	59277.0
33	77432.0
34	101899.0
35	135078.0
36	191889.0
37	269553.0
38	663265.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52443407437466	17.986849242465023	12.15865378643269	25.33006289672763
2	13.682617322880263	19.47458426701262	42.44790535130136	24.39489305880576
3	19.757472442048524	24.231343526745423	28.551035934854983	27.460148096351073
4	10.787781932527553	16.290360118539667	38.91498968208406	34.00686826684872
5	12.278843448469903	38.08278578639877	36.30313756806408	13.335233197067245
6	32.71863497352872	35.9615863944143	18.10849552744501	13.21128310461197
7	28.80264020668243	31.119078202988128	21.36713021282581	18.711151377503636
8	25.733218736392903	36.87766776609399	19.996545543492537	17.392567954020564
9	26.758290775098587	15.340668433641477	19.62159157023725	38.279449221022695
10	14.126813777337578	29.49435340179419	34.834610401533	21.544222419335235
11	34.5272367071765	22.896788602982053	23.215544359228016	19.36043033061343
12	23.440442737717618	25.158006108225006	31.333611888802587	20.067939265254793
13	29.156699523335266	21.308485542341874	25.146417464813787	24.388397469509073
14	20.63807099721476	22.259570185844616	28.126981237647158	28.97537757929346
15	24.57361410686887	29.511059582066583	22.80735957487849	23.107966736186057
16	21.770551926426357	28.209693567270815	28.438680357608277	21.581074148694547
17	22.312643445145127	28.330487271328142	28.05913741049385	21.297731873032884
18	21.826163264820668	27.082489788256613	30.116149369920308	20.97519757700241
19	23.285693893931995	27.415347505523513	27.937960809949768	21.36099779059472
20	23.36799525889397	27.231779476513218	28.154299006167367	21.245926258425442
21	22.633398805774398	26.90674262711014	27.833493135736887	22.626365431378574
22	22.668903752221397	27.83428533598694	26.89876429296994	22.598046618821723
23	21.00218137586999	27.336756235125222	29.24196929938427	22.41909308962052
24	22.475553353325807	28.06349869710985	27.665442222330267	21.795505727234076
25	22.491943612788646	26.83243697244305	27.463759833550462	23.211859581217848
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	323.0
3	481.0
4	481.0
5	481.0
6	943.5
7	1406.0
8	1406.0
9	1406.0
10	2271.0
11	3136.0
12	3136.0
13	3136.0
14	5511.0
15	7886.0
16	7886.0
17	7886.0
18	14102.5
19	20319.0
20	20319.0
21	20319.0
22	32290.0
23	44261.0
24	44261.0
25	44261.0
26	63244.0
27	82227.0
28	82227.0
29	82227.0
30	106264.5
31	130302.0
32	130302.0
33	130302.0
34	151332.5
35	172363.0
36	172363.0
37	172363.0
38	191694.5
39	211026.0
40	211026.0
41	211026.0
42	227131.0
43	243236.0
44	243236.0
45	243236.0
46	268764.5
47	294293.0
48	294293.0
49	294293.0
50	276020.5
51	257748.0
52	257748.0
53	257748.0
54	222162.0
55	186576.0
56	186576.0
57	186576.0
58	157483.0
59	128390.0
60	128390.0
61	128390.0
62	103407.0
63	78424.0
64	78424.0
65	78424.0
66	59854.5
67	41285.0
68	41285.0
69	41285.0
70	29889.5
71	18494.0
72	18494.0
73	18494.0
74	13041.0
75	7588.0
76	7588.0
77	7588.0
78	5298.0
79	3008.0
80	3008.0
81	3008.0
82	2031.5
83	1055.0
84	1055.0
85	1055.0
86	692.5
87	330.0
88	330.0
89	330.0
90	225.5
91	121.0
92	121.0
93	121.0
94	72.5
95	24.0
96	24.0
97	24.0
98	16.0
99	8.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10836790490095918
2	0.048600110022376734
3	0.0352092286438708
4	0.032158796206296095
5	0.035157526399166146
6	0.018509403604266884
7	0.01881961707249482
8	0.021611538286546248
9	0.027247082959353762
10	0.03551944211209874
11	0.019801959721883287
12	0.018923021561904133
13	0.015407268921987518
14	0.019957066455997253
15	0.02062919563715778
16	0.021508133797136935
17	0.02512729092646286
18	0.01804408340192498
19	0.031021346822793654
20	0.022025156244183497
21	0.026574953778193234
22	0.03536433537798477
23	0.026368144799374608
24	0.03919030148612932
25	0.030659431109861066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1934152.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.7767682228538
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.49552120368828	66.30127830789677
2	8.918219662015185	13.515877285780444
3	1.976412289468576	4.492984079155804
4	0.6472224369228727	1.9617769836534054
5	0.30032529638871197	1.137884018795365
6	0.15804756523474164	0.7185800231387629
7	0.10361619202971721	0.5496190117299414
8	0.06876236779336395	0.41684720053858937
9	0.05248494939141402	0.3579425860699253
>10	0.2476708411177448	3.2798976087427016
>50	0.017034849560806677	0.90318172480527
>100	0.011220488710188712	1.705913537517614
>500	0.001800165992775547	1.047477248962469
>1k	0.0014539802249340938	2.1579133107851893
>5k	1.3847430713658036E-4	0.7906577581700172
>10k+	6.923715356829018E-5	0.6621693142577164
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	12621	0.6525340304174646	No Hit
CCCATGTACTCTGCGTTGATACCAC	9841	0.5088017901385207	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5229	0.27035103756064677	No Hit
GTATCAACGCAGAGTACATGGGAAG	3537	0.18287083952036862	No Hit
GCGTTGATACCACTGCTTCCCATGT	3356	0.17351273322882588	No Hit
CATGTACTCTGCGTTGATACCACTG	3053	0.1578469530833151	No Hit
TATCAACGCAGAGTACATGGGAAGC	2735	0.14140563926723443	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2709	0.14006138090491338	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2650	0.13701094846733866	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2400	0.12408538729117462	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2216	0.1145721742655179	No Hit
GTCCTACAGTGGACATTTCTAAATT	1948	0.10071597268467007	No Hit
GGTATCAACGCAGAGTACATGGGAA	1946	0.10061256819526078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.1702244704656095E-5
2	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
3	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
4	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
5	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
6	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
7	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
8	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
9	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
10	0.0	0.0	0.0	5.1702244704656095E-5	5.1702244704656095E-5
11	0.0	0.0	0.0	1.0340448940931219E-4	5.1702244704656095E-5
12	0.0	0.0	0.0	1.0340448940931219E-4	5.1702244704656095E-5
13	0.0	0.0	0.0	1.0340448940931219E-4	5.1702244704656095E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGAC	60	2.5682391E-5	14.250389	19
TAGGACC	570	0.0	13.502112	4
GCGTTTA	205	0.0	13.43661	13
CGTTTAT	205	0.0	13.43661	14
GATACGA	50	0.0014994702	13.300363	18
CTAGGAC	290	0.0	12.448936	3
TACCCCG	55	0.003066503	12.092176	5
TAGGACA	530	0.0	11.832042	4
GTAGGAC	1280	0.0	11.801408	3
GTCCTAC	1340	0.0	11.780862	1
GTCCTAT	205	0.0	11.597381	1
TCCTACA	1550	0.0	11.278602	2
TGTAGGA	1450	0.0	11.270147	2
ATACCGA	60	0.005877607	11.083063	6
TCCAACG	155	3.1286618E-10	11.032558	18
ACAGCGT	95	1.3620995E-5	10.998878	8
TGCGTTT	270	0.0	10.905448	12
GGCGAGG	410	0.0	10.89054	19
ACCGTGC	115	8.830448E-7	10.738033	8
CGGGAGT	80	3.7661183E-4	10.689174	4
>>END_MODULE