FastQCFastQC Report
Thu 26 May 2016
SRR1513058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934152
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC141190.7299839929850395No Hit
CCCATGTACTCTGCGTTGATACCAC136490.7056839379738511No Hit
GAGTACATGGGAAGCAGTGGTATCA50690.2620786784079017No Hit
GTATCAACGCAGAGTACTTTTTTTT40060.20711919228685233No Hit
CATGTACTCTGCGTTGATACCACTG39020.20174215883756808No Hit
GCGTTGATACCACTGCTTCCCATGT36140.18685191236262716No Hit
GTATCAACGCAGAGTACATGGGAAG35120.1815782834027522No Hit
ACGCAGAGTACATGGGAAGCAGTGG33670.17408145792057708No Hit
GCTTCCCATGTACTCTGCGTTGATA33610.17377124445234912No Hit
TATCAACGCAGAGTACATGGGAAGC31450.16260355959614342No Hit
ACTCTGCGTTGATACCACTGCTTCC28630.1480235265894304No Hit
TATCAACGCAGAGTACTTTTTTTTT27020.1396994651919808No Hit
GGTATCAACGCAGAGTACTTTTTTT23810.12310304464178616No Hit
GGTATCAACGCAGAGTACATGGGAA22700.11736409547956933No Hit
GTCCTACAGTGGACATTTCTAAATT22340.11550281467020172No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTGCG402.777527E-416.61243416
TTGCGCG350.002186730516.26751118
GCCGGTT551.983573E-413.79916811
TAGGACC5500.013.5009734
GCACCGT957.190647E-813.0272556
GAACCGT701.0700368E-412.2398936
TGCGCGC550.00310072112.07427119
GTCTAGG1604.3655746E-1111.3157661
GGTCTAC1604.3655746E-1111.3157661
GTCCTAC12350.011.1108141
AGGACCT10300.011.0911665
TGCCCGT600.005943929311.06636610
AGGACGT9500.011.0230625
TGTAGGA12750.010.9830292
GTAGGAC12350.010.9459773
GGACGTG9550.010.86566456
TAGACTG3700.010.8063925
TGGCGAG11250.010.79680718
TTAGGAC6650.010.7367483
GTATAAG2500.010.6725541
Produced by FastQC (version 0.11.5)