FastQCFastQC Report
Thu 26 May 2016
SRR1513057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084402
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC187540.8997304742559257No Hit
CCCATGTACTCTGCGTTGATACCAC148390.7119068202774705No Hit
GAGTACATGGGAAGCAGTGGTATCA76850.36869087632807873No Hit
GTATCAACGCAGAGTACATGGGAAG52240.25062344020011496No Hit
GCGTTGATACCACTGCTTCCCATGT50420.24189191912116761No Hit
CATGTACTCTGCGTTGATACCACTG47670.22869868672165927No Hit
ACGCAGAGTACATGGGAAGCAGTGG39680.19036634967726956No Hit
TATCAACGCAGAGTACATGGGAAGC39240.1882554324933482No Hit
GCTTCCCATGTACTCTGCGTTGATA37860.1816348285983222No Hit
ACTCTGCGTTGATACCACTGCTTCC34550.16575497432836853No Hit
GGTATCAACGCAGAGTACATGGGAA25090.12037025487405979No Hit
GTATCAACGCAGAGTACTTTTTTTT20910.10031654162680712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAC602.569823E-514.24956919
CGTGCGC1101.8735591E-1013.81643710
ATACGAC1051.371518E-913.57101619
GCGTTTA2300.012.80211613
GTCTAAC905.319289E-712.6839381
TGATACG905.417787E-712.664762517
AAGGCGT1701.8189894E-1211.7343756
GATACGA1302.6175258E-911.69223418
GTCCTAC7550.011.4659441
GTCCTAA3850.011.3661271
GGTATCG855.3328047E-511.1747917
TCTATAC2300.011.1534423
CGCAGAT1201.2777127E-711.08228
TCCTACA8350.011.0369192
TCCAACG1303.2679964E-810.9614718
GACTGCG700.001493045210.8560327
TAGGACT2300.010.740614
AAAGGCG2050.010.6587265
GTCTTAG3000.010.464251
CGTTTAT2750.010.36183114
Produced by FastQC (version 0.11.5)