##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513057_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2084402
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.20852263622852	31.0	30.0	34.0	24.0	34.0
2	28.931897014107644	31.0	30.0	34.0	16.0	34.0
3	29.275770220907482	31.0	30.0	34.0	23.0	34.0
4	32.10854767938238	35.0	32.0	37.0	19.0	37.0
5	32.35287003178849	35.0	33.0	37.0	22.0	37.0
6	32.783168985637126	36.0	33.0	37.0	26.0	37.0
7	32.78528613962182	37.0	33.0	37.0	26.0	37.0
8	32.7654296052297	37.0	35.0	37.0	26.0	37.0
9	34.118307313080685	39.0	35.0	39.0	25.0	39.0
10	33.61738186779709	38.0	34.0	39.0	18.0	39.0
11	34.002348395367115	39.0	34.0	39.0	25.0	39.0
12	33.83393414514091	39.0	34.0	39.0	23.0	39.0
13	33.92255092827583	39.0	34.0	39.0	24.0	39.0
14	34.21823045650503	39.0	33.0	41.0	16.0	41.0
15	34.763055303151695	40.0	34.0	41.0	22.0	41.0
16	34.34110022922641	39.0	34.0	41.0	17.0	41.0
17	33.873587724440874	39.0	33.0	41.0	11.0	41.0
18	33.27969029006881	39.0	32.0	41.0	10.0	41.0
19	34.16436176898698	39.0	33.0	41.0	18.0	41.0
20	34.436867264567965	39.0	34.0	41.0	18.0	41.0
21	34.451905630487786	39.0	34.0	41.0	18.0	41.0
22	34.045422140258935	39.0	34.0	41.0	10.0	41.0
23	32.36714319022914	39.0	31.0	41.0	2.0	41.0
24	33.44950590145279	39.0	33.0	41.0	2.0	41.0
25	33.189842938166436	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7549806074127936
1101	2	1.1750753443859523
1101	3	0.9060687714987559
1101	4	0.8694824889411876
1101	5	1.1122478213811178
1101	6	0.7323930179212539
1101	7	0.7468879004596829
1101	8	0.8662249158314523
1101	9	0.9862802008717182
1101	10	1.2702136055913869
1101	11	0.9476750763623727
1101	12	1.0211028376005302
1101	13	0.8356339418575516
1101	14	0.0414062971760103
1101	15	0.6494290584365743
1101	16	1.213232750232784
1101	17	1.8926021625103004
1101	18	-4.340989286959296
1101	19	-0.7667323658867247
1101	20	0.2153649890417455
1101	21	0.6175915226152924
1101	22	1.2175510157513187
1101	23	3.035399006695684
1101	24	1.9434380417149484
1101	25	2.184673766042117
1102	1	0.9905894043080075
1102	2	1.180445279702898
1102	3	0.97515420487521
1102	4	1.5621492935983667
1102	5	1.2903209908507165
1102	6	0.9369568471579939
1102	7	0.8831858564752011
1102	8	0.7771543854304213
1102	9	0.8534384932391177
1102	10	1.3624142782951338
1102	11	1.0427844165952322
1102	12	1.1146347392822946
1102	13	0.8935646016246892
1102	14	1.8575246930362965
1102	15	1.302285255073059
1102	16	1.5189315859378283
1102	17	1.2476283979566105
1102	18	1.67415079066037
1102	19	1.3224272718881807
1102	20	1.2921275246174204
1102	21	1.2979312897563062
1102	22	1.6583947932416194
1102	23	3.283812771249373
1102	24	2.148930823086232
1102	25	2.4406012175298315
1103	1	0.712372290972521
1103	2	0.963970807633153
1103	3	0.8744003915074359
1103	4	1.332813755981313
1103	5	1.1075648268210116
1103	6	0.792229123554506
1103	7	0.7469725920257346
1103	8	0.6898496647329679
1103	9	0.8840439049278501
1103	10	1.1457298482014977
1103	11	0.9422454175639103
1103	12	1.0348251687301442
1103	13	0.9367206881600438
1103	14	1.6782772664944758
1103	15	1.1704990878144201
1103	16	1.354019477445739
1103	17	1.7847488629227044
1103	18	1.8389264057717654
1103	19	1.3358901068897993
1103	20	1.153530120678127
1103	21	1.1585677250162334
1103	22	1.4792332304389078
1103	23	2.9646116863284817
1103	24	1.907516912850518
1103	25	2.0561120731966014
1104	1	0.6665124125935407
1104	2	1.0561222204294758
1104	3	0.8430005273736185
1104	4	1.3759897524893816
1104	5	1.1724677849871128
1104	6	0.7353484851439731
1104	7	0.6534567551367729
1104	8	0.6948564631869516
1104	9	0.7409782765405808
1104	10	1.1546492304939875
1104	11	0.7203634095844009
1104	12	0.8877638151267817
1104	13	0.6526524282658599
1104	14	1.5019488103084413
1104	15	1.0306705195727375
1104	16	1.3456584183151392
1104	17	1.829186179983381
1104	18	2.3645014343824826
1104	19	1.5764672973821234
1104	20	1.005287295171918
1104	21	1.0616674784115006
1104	22	1.5379953541084106
1104	23	2.886737232689782
1104	24	2.0216154884782043
1104	25	2.185364914151883
1105	1	0.2961944756233805
1105	2	0.8400631560895881
1105	3	0.4298184204061144
1105	4	0.5541751573067657
1105	5	0.8296675518649792
1105	6	0.3422619501690889
1105	7	0.2855582178064253
1105	8	0.1604403442259823
1105	9	0.39994575155924394
1105	10	0.9107745952523487
1105	11	0.4970642264209104
1105	12	0.46239461249940916
1105	13	0.37427965709220246
1105	14	1.1699978467605376
1105	15	0.5342781552315401
1105	16	0.9962463244481299
1105	17	1.3510259256903652
1105	18	1.3770792217221768
1105	19	1.015059918480894
1105	20	0.7551010903860416
1105	21	0.5353352834171616
1105	22	0.9986019564745803
1105	23	2.52127001372164
1105	24	1.520832186002238
1105	25	1.5602173189561341
1106	1	-0.6829473566174435
1106	2	-0.4310181650271758
1106	3	-0.6599042012944558
1106	4	-2.9721795495892955
1106	5	-0.9144272867842425
1106	6	-0.8157272659525461
1106	7	-0.8710268941674677
1106	8	-0.7340117842353919
1106	9	-0.7259637689716811
1106	10	-0.5630462872575634
1106	11	-0.7693116245358738
1106	12	-0.5977942581470117
1106	13	-0.738189125881739
1106	14	0.07114844624965855
1106	15	-0.5710029711480189
1106	16	-1.5277486197176984
1106	17	-6.240780445231049
1106	18	-7.544461137360198
1106	19	-3.3855346818393244
1106	20	-1.9102810100869476
1106	21	-1.4670443400637438
1106	22	-1.6511124561157047
1106	23	-5.445292935691501
1106	24	-2.621147737325078
1106	25	-1.1060146179457533
1107	1	0.3864925322113848
1107	2	0.8038562180739106
1107	3	0.4915413464050964
1107	4	0.9246921489715447
1107	5	0.8760791574657674
1107	6	0.4509932918777224
1107	7	0.31486149599208346
1107	8	0.43215375365587505
1107	9	0.37366107608210086
1107	10	0.9190157772112073
1107	11	0.49677730603468717
1107	12	0.5109284277307253
1107	13	0.4776899655144362
1107	14	1.3684020668362322
1107	15	0.8087527930104983
1107	16	-5.621932334780659
1107	17	-1.1399756404453711
1107	18	0.6520270867272586
1107	19	0.6037592012780877
1107	20	0.5795734459552975
1107	21	0.4722370438597423
1107	22	0.6151902345547171
1107	23	2.5054961654890775
1107	24	1.5778177354429985
1107	25	1.2199594832467682
1108	1	0.43385835234349557
1108	2	0.5815640629728627
1108	3	0.39346254454568097
1108	4	0.9794928677428487
1108	5	0.7941104965541399
1108	6	0.4344205733937372
1108	7	0.41037247244478436
1108	8	0.4174298733898638
1108	9	0.635516465355721
1108	10	0.9628830235422825
1108	11	0.5380932125394651
1108	12	0.5776222171023164
1108	13	0.44230072329769143
1108	14	1.0611731594494742
1108	15	0.724603227189661
1108	16	0.9184960979783838
1108	17	1.055949006321633
1108	18	0.8331380656920544
1108	19	0.8055486914065568
1108	20	0.5921605791167863
1108	21	0.7083273353836788
1108	22	1.0381789250540407
1108	23	2.7152080564204795
1108	24	1.5315967799937127
1108	25	1.67783244693414
1109	1	0.27640055672436503
1109	2	0.5891061966793565
1109	3	0.4344784180102792
1109	4	0.45693125146701163
1109	5	0.3591226761047466
1109	6	0.2428941953612167
1109	7	0.162171135285611
1109	8	0.13977852421604808
1109	9	0.22728949695658685
1109	10	0.7698689499184326
1109	11	0.4012110832618987
1109	12	0.4757333266428887
1109	13	0.3046505991463775
1109	14	-0.7593398422796938
1109	15	-0.0017773967237175725
1109	16	-0.20348080563589832
1109	17	0.8670788068785455
1109	18	1.7319382503877563
1109	19	0.9571687836601797
1109	20	0.7115637575790075
1109	21	0.6909402612556832
1109	22	0.5497353632540083
1109	23	-4.265905394797716
1109	24	-1.358959238871634
1109	25	-0.08720706004142187
1110	1	0.7481408529203897
1110	2	1.0742598329203439
1110	3	0.7687467879798149
1110	4	1.3420781987523753
1110	5	1.068752561451511
1110	6	0.7554135961668393
1110	7	0.6006877289973147
1110	8	0.6649570569766254
1110	9	0.6909446597532991
1110	10	1.0404851047666241
1110	11	0.7027511869520495
1110	12	0.7797571302551489
1110	13	0.6208902496881237
1110	14	1.4987906399713324
1110	15	0.9797112020080974
1110	16	1.3277240226563407
1110	17	1.7064446712106545
1110	18	2.359663007799739
1110	19	1.4697834170543942
1110	20	1.0927676222081502
1110	21	1.064277517264692
1110	22	1.2386779527414689
1110	23	2.646457382411313
1110	24	1.7076755135406358
1110	25	1.9145859201830788
1111	1	0.48382603256755985
1111	2	0.7913340122680914
1111	3	0.5402800265853394
1111	4	1.1937401244049113
1111	5	0.7613887410023281
1111	6	0.30312426983840624
1111	7	0.25513724519564107
1111	8	-0.0010422428291292363
1111	9	0.12188259269333201
1111	10	0.48535026374086243
1111	11	0.22863174224714555
1111	12	0.38663347791176506
1111	13	0.2834522911334858
1111	14	-0.5463308315240454
1111	15	-0.11513737682506076
1111	16	0.35243213172315535
1111	17	1.0436959297940902
1111	18	1.624200129653694
1111	19	0.8206715411422749
1111	20	0.4387407932650902
1111	21	0.43851811813713937
1111	22	0.7634298933022592
1111	23	0.7871251302548288
1111	24	0.6409496466156455
1111	25	0.12408881001012162
1112	1	0.12036055815416091
1112	2	0.5040046066259762
1112	3	0.2534746856444414
1112	4	0.8187082359060582
1112	5	0.5325745485430247
1112	6	-0.07465440284200753
1112	7	-0.03863491275556186
1112	8	-0.08439167226188715
1112	9	-0.09501131445318833
1112	10	0.2571016821412897
1112	11	-0.0980877949432255
1112	12	-0.09082372225616808
1112	13	-0.15466087914454363
1112	14	0.17871978919013998
1112	15	-0.0723308026192484
1112	16	0.2992238936301348
1112	17	0.5930760902643044
1112	18	0.6777571368142148
1112	19	0.09209842573433491
1112	20	0.06863358230972949
1112	21	0.14608782821784416
1112	22	0.15050998554931283
1112	23	1.74117507894168
1112	24	0.7444917846246923
1112	25	0.7541621790682882
1113	1	0.35359711944153815
1113	2	0.7602487218393996
1113	3	0.4179739081393059
1113	4	0.5568692935983677
1113	5	0.7012152341741569
1113	6	0.31297773736570633
1113	7	0.19548828970962262
1113	8	0.07280684833843054
1113	9	0.2617119650492086
1113	10	0.4907903020340072
1113	11	0.21154738395428296
1113	12	0.28718358201226124
1113	13	0.1294767677967954
1113	14	0.744507244965078
1113	15	0.34886994350036105
1113	16	0.6542170458784824
1113	17	1.1217995552266373
1113	18	1.7002744701855903
1113	19	0.7997044232234956
1113	20	0.5403668124512535
1113	21	0.5754026250678734
1113	22	0.6708738436867208
1113	23	0.4974479047805289
1113	24	0.44018162427317264
1113	25	0.9250745706455561
1114	1	-0.42360246561069204
1114	2	-2.713620597184441
1114	3	-1.0703594768901787
1114	4	-0.056022692464352986
1114	5	0.17286696065327334
1114	6	-0.07428691590821046
1114	7	-0.1165658740706732
1114	8	-0.09884022548711613
1114	9	-0.023608568317214917
1114	10	-0.01481666247141078
1114	11	-0.16382956714460306
1114	12	-0.04333868696625842
1114	13	-0.10832018350887296
1114	14	0.6626644839770606
1114	15	-0.04892366478756571
1114	16	0.2622923292595445
1114	17	0.6669559705466241
1114	18	0.781543403900784
1114	19	0.11237756224822704
1114	20	-0.06093644750801275
1114	21	-0.15534917481976862
1114	22	0.039403294983777926
1114	23	1.8998373008660963
1114	24	0.8089314734927342
1114	25	0.7460118563219282
1115	1	0.030286154379908226
1115	2	0.25678722965976064
1115	3	0.011352950920652916
1115	4	0.18675137585318424
1115	5	0.18623429542362402
1115	6	-0.2606882952061156
1115	7	-0.3281203500246548
1115	8	-0.31008505086523996
1115	9	-0.2570868562000541
1115	10	-0.07218419165021572
1115	11	-0.12301957535184727
1115	12	-0.34750499036970695
1115	13	-0.35531937881822273
1115	14	0.4843120154406648
1115	15	-0.11874786543312865
1115	16	0.31167107400224836
1115	17	0.7471697496966172
1115	18	1.1563691628202761
1115	19	0.2638337717443804
1115	20	-0.02095920532217832
1115	21	-0.03352898059169718
1115	22	0.3145012758415646
1115	23	1.8157897168921693
1115	24	0.8099679010269867
1115	25	0.3739235988930716
1116	1	0.23591977937403996
1116	2	0.3320335734165809
1116	3	0.43710843625397544
1116	4	1.089832843519126
1116	5	0.5125518624830505
1116	6	0.35361958355524337
1116	7	0.16685774237060969
1116	8	0.15336163318530538
1116	9	0.1910181234557058
1116	10	0.7701512460711513
1116	11	0.32051906001836983
1116	12	0.4807253045226503
1116	13	0.2471269893690078
1116	14	1.0040179207172244
1116	15	0.36026864651521606
1116	16	0.8088189604755982
1116	17	1.0515541877083265
1116	18	1.5867992428526563
1116	19	0.6800889728918804
1116	20	0.5428454115693526
1116	21	0.453877280247589
1116	22	0.8298222417360748
1116	23	2.14546441414425
1116	24	1.0755073048611905
1116	25	1.294765654930778
1201	1	1.1247257649956488
1201	2	1.3555564830762385
1201	3	1.1707261023776425
1201	4	1.7070152553239595
1201	5	1.5525675818335287
1201	6	1.0450228088835871
1201	7	1.0160828010097518
1201	8	1.0760275349600974
1201	9	1.1543393819838457
1201	10	1.3425934803710362
1201	11	0.961514899890723
1201	12	1.1732609604953979
1201	13	1.2064032652640009
1201	14	1.947633703740152
1201	15	1.3996862800487335
1201	16	1.7378419978742556
1201	17	2.1239479308790905
1201	18	2.7036561815134306
1201	19	1.844659383467814
1201	20	1.658214375087745
1201	21	1.4784653669538415
1201	22	1.7851236158170352
1201	23	3.3529504164001978
1201	24	2.1964532622688466
1201	25	2.333191447824994
1202	1	1.226780541109548
1202	2	1.5445424922912103
1202	3	1.2683877565772015
1202	4	1.8886612011128179
1202	5	1.653990200869984
1202	6	1.1380367700866998
1202	7	1.071857096038464
1202	8	1.1249820540238673
1202	9	1.2904698098736702
1202	10	1.7191810862591694
1202	11	1.4100421686825797
1202	12	1.4357436410163942
1202	13	1.2912180377197444
1202	14	2.265527467602766
1202	15	1.684469866505303
1202	16	1.9774821318594746
1202	17	2.415821384469133
1202	18	3.001937868373922
1202	19	2.305334400982595
1202	20	1.8526766832558295
1202	21	1.770248720713269
1202	22	2.18739404821914
1202	23	3.7528670037477667
1202	24	2.6175260189757665
1202	25	2.8251732920320762
1203	1	0.8965511926643934
1203	2	1.2485109985835692
1203	3	0.932230975541021
1203	4	1.5776798436144475
1203	5	1.383553387119612
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2211	17	0.3773850696479002
2211	18	-5.23970785269206
2211	19	-1.583138480297528
2211	20	-0.8466236843063015
2211	21	-0.4670788278198188
2211	22	-0.13447133880541173
2211	23	-4.788319685790121
2211	24	-2.025252981556516
2211	25	-1.3389922946800006
2212	1	0.18922527056940552
2212	2	0.4600863739278225
2212	3	0.21742192523995385
2212	4	0.739240114267055
2212	5	0.5894125410026945
2212	6	0.139861649589605
2212	7	-0.004909462673730047
2212	8	-0.07695819815620553
2212	9	-0.3601581025055651
2212	10	-3.981530280975381
2212	11	-1.1884039116722462
2212	12	-0.42244635494262894
2212	13	-0.21324147740266852
2212	14	0.5530232948600045
2212	15	0.08835115172959007
2212	16	0.3542215555426935
2212	17	0.9065054800234833
2212	18	1.4443834958275446
2212	19	0.47422301656903443
2212	20	0.1880998042526798
2212	21	0.11861603139764298
2212	22	0.19348439810484308
2212	23	1.4401886983010428
2212	24	0.6835564765816713
2212	25	0.5936827980769408
2213	1	-1.4386612591419308
2213	2	-3.681808134048609
2213	3	-1.6350194018799158
2213	4	-3.2550124916851075
2213	5	-1.2240443649997026
2213	6	-0.936003731852864
2213	7	-0.984610717710563
2213	8	-0.8828372189121616
2213	9	-0.7552868143603959
2213	10	-0.837076385154802
2213	11	-0.867084051438539
2213	12	-0.7571501581845368
2213	13	-0.979884373043582
2213	14	-6.057856440859968
2213	15	-2.7467187497520342
2213	16	-1.9341318640018557
2213	17	-0.6979197805717376
2213	18	0.06127571225264461
2213	19	-0.5558106050720113
2213	20	-0.8633189410038042
2213	21	-1.037635320617639
2213	22	-0.7668712139960192
2213	23	-5.650918615964137
2213	24	-3.0751682842720314
2213	25	-2.2949912553290375
2214	1	-0.2210223603978747
2214	2	0.002513514997016131
2214	3	-0.20579638336008088
2214	4	0.028045764186600763
2214	5	-0.09869312679634135
2214	6	-0.334240799900833
2214	7	-0.5103600258777377
2214	8	-0.6191350263342841
2214	9	-0.48888150676088316
2214	10	-0.017378662881334606
2214	11	-0.4810438186988861
2214	12	-0.5060499666000595
2214	13	-0.5144706924929565
2214	14	-0.17248001284605863
2214	15	-0.5659712155151766
2214	16	0.06829629182018238
2214	17	0.3229742803095519
2214	18	0.8390849083074272
2214	19	-0.0920786104647604
2214	20	-0.28191718126493015
2214	21	-0.44380753377311066
2214	22	0.007044205006323523
2214	23	1.5614974771317236
2214	24	0.5515378819097592
2214	25	0.47826709988277116
2215	1	-2.6719517318609896
2215	2	-4.5745677262919635
2215	3	-2.5656774936688933
2215	4	-4.2787287652083705
2215	5	-5.18400277734797
2215	6	-2.7066361417086924
2215	7	-2.059575068446293
2215	8	-2.3643909414322692
2215	9	-2.1491584122704452
2215	10	-3.739793063982912
2215	11	-2.524162406128145
2215	12	-2.1222198881798846
2215	13	-2.2472673709702278
2215	14	-1.4851115387650182
2215	15	-2.0187499262001367
2215	16	-3.0548929073536364
2215	17	-7.407592292879816
2215	18	-3.589818972399865
2215	19	-2.6208711580290327
2215	20	-2.50199518163436
2215	21	-2.424386449324487
2215	22	-2.0576613415000296
2215	23	-6.468788496235639
2215	24	-4.021667269976856
2215	25	-3.8042463759614655
2216	1	-1.1899770416081061
2216	2	-0.8155802390608962
2216	3	-1.0557385986567986
2216	4	-3.638715695364109
2216	5	-1.6083217464561237
2216	6	-1.287187170502051
2216	7	-1.3158307439662948
2216	8	-1.2127248198675922
2216	9	-1.2114532506902407
2216	10	-0.9382817923450375
2216	11	-1.4950361573562034
2216	12	-2.3108525896360312
2216	13	-1.5433987537836984
2216	14	-7.115704269436112
2216	15	-3.5059205727415126
2216	16	-1.930377906335906
2216	17	-1.1128905424407378
2216	18	-0.3597081055868756
2216	19	-0.9871409400323472
2216	20	-1.449280510702664
2216	21	-1.6529628382790165
2216	22	-1.0172909462727304
2216	23	0.019644471028620103
2216	24	-0.7158222011963318
2216	25	-0.7133261996887157
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101600.0
3	4.0
4	885.0
5	5165.0
6	6339.0
7	5724.0
8	5216.0
9	4814.0
10	4669.0
11	4534.0
12	4565.0
13	4409.0
14	4466.0
15	4595.0
16	4671.0
17	5113.0
18	5687.0
19	6716.0
20	8366.0
21	10368.0
22	12932.0
23	15465.0
24	18417.0
25	21720.0
26	24732.0
27	28143.0
28	31506.0
29	38089.0
30	44862.0
31	51329.0
32	65765.0
33	86003.0
34	111588.0
35	146205.0
36	205369.0
37	286304.0
38	698067.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.202087521224655	17.496703724251223	11.878697052445009	25.422511702079113
2	13.92050486147076	19.49901676231855	41.19504296535077	25.38543541085992
3	19.954302135734466	24.202159058514574	28.30709617475954	27.536442630991427
4	11.075539780675633	16.01880828098013	38.07870664754101	34.82694529080322
5	12.552971134750887	37.7440511555986	36.02027564666777	13.682702062982738
6	33.43592519335542	34.977442266886634	17.925792207817135	13.66084033194081
7	28.982697713341928	30.849346593717282	21.193501733683558	18.974453959257236
8	26.620183979308326	35.66952824711003	19.974471311416412	17.735816462165236
9	27.130423265315724	15.219670768582553	19.22452146847405	38.42538449762768
10	14.492753623188406	29.277349205389356	34.20559572521839	22.024301446203847
11	34.65884771371922	23.070773648317942	22.382096702680812	19.888281935282027
12	23.70004049927351	25.1307109253988	30.62973491210122	20.53951366322647
13	29.18185325295239	20.891080747535117	24.86358462935133	25.063481370161167
14	21.22762626963017	21.700329130943636	27.72727861637363	29.34476598305256
15	25.571856260127955	28.090331911520334	22.60802635752431	23.729785470827398
16	22.383026953933445	27.664934550181552	27.758026414096605	22.1940120817884
17	23.11754272815221	27.520548287034902	27.64234079636369	21.719568188449195
18	22.676459340107783	26.586283059509874	29.116696824914694	21.620560775467645
19	23.680772848559307	27.313650586451153	27.643013456702437	21.3625631082871
20	23.82625261223305	26.82465228208998	27.34448365959754	22.00461144607943
21	23.032071929575356	26.669149291492143	27.69942259777255	22.599356181159948
22	23.10800395076707	26.991677089102467	26.127950717156367	23.772368242974096
23	21.23968124018032	26.807324603877674	28.75364833724121	23.19934581870079
24	22.860176111109247	28.202206692260546	26.561801829647276	22.375815366982934
25	23.058745955045882	26.165280316580198	26.430424869551278	24.345548858822642
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	194.0
1	194.0
2	308.5
3	423.0
4	423.0
5	423.0
6	860.0
7	1297.0
8	1297.0
9	1297.0
10	1989.0
11	2681.0
12	2681.0
13	2681.0
14	4729.0
15	6777.0
16	6777.0
17	6777.0
18	12865.5
19	18954.0
20	18954.0
21	18954.0
22	30104.5
23	41255.0
24	41255.0
25	41255.0
26	59110.0
27	76965.0
28	76965.0
29	76965.0
30	100213.5
31	123462.0
32	123462.0
33	123462.0
34	146522.5
35	169583.0
36	169583.0
37	169583.0
38	190706.5
39	211830.0
40	211830.0
41	211830.0
42	233905.0
43	255980.0
44	255980.0
45	255980.0
46	293508.0
47	331036.0
48	331036.0
49	331036.0
50	311588.5
51	292141.0
52	292141.0
53	292141.0
54	253104.5
55	214068.0
56	214068.0
57	214068.0
58	181880.0
59	149692.0
60	149692.0
61	149692.0
62	122412.5
63	95133.0
64	95133.0
65	95133.0
66	73576.5
67	52020.0
68	52020.0
69	52020.0
70	38262.0
71	24504.0
72	24504.0
73	24504.0
74	17254.5
75	10005.0
76	10005.0
77	10005.0
78	7039.0
79	4073.0
80	4073.0
81	4073.0
82	2796.5
83	1520.0
84	1520.0
85	1520.0
86	1053.5
87	587.0
88	587.0
89	587.0
90	380.5
91	174.0
92	174.0
93	174.0
94	106.0
95	38.0
96	38.0
97	38.0
98	24.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12027430409297248
2	0.046967907342249725
3	0.03435037962926537
4	0.030656274557403034
5	0.03454228119144004
6	0.019957762466165357
7	0.01919015621746669
8	0.022644384336610694
9	0.02777775112478303
10	0.03550178900231338
11	0.019478008560728687
12	0.019861811685078022
13	0.01563997731723535
14	0.020581442543233023
15	0.021253098010844358
16	0.02106119644866969
17	0.02542695698814336
18	0.018950279264748356
19	0.032719216350780705
20	0.02192475347845569
21	0.028113578858588697
22	0.03626939525101204
23	0.027633824953152034
24	0.03986754954178704
25	0.030368422214141034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2084402.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.87048364238687
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.1325398316908	61.185168114083844
2	10.113655347742363	14.537466024693119
3	2.547600973574237	5.492919422957882
4	0.9125358709321305	2.6233757753967577
5	0.42280615617408007	1.5193641465604846
6	0.23277909874028269	1.0037967844981828
7	0.1401269381455	0.7049693571090742
8	0.09641122665372821	0.5543297190527382
9	0.0711785745732755	0.46040747216013106
>10	0.3001379568504824	3.7416672742140356
>50	0.014995687403244936	0.7495105054241877
>100	0.011494208219699684	1.5559486349600926
>500	0.0014952517200455622	0.756012506257528
>1k	0.0019030476436942812	2.597269559360207
>5k	2.0389796182438726E-4	0.8768611810963697
>10k+	1.3593197454959152E-4	1.6409335221753856
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	18754	0.8997304742559257	No Hit
CCCATGTACTCTGCGTTGATACCAC	14839	0.7119068202774705	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7685	0.36869087632807873	No Hit
GTATCAACGCAGAGTACATGGGAAG	5224	0.25062344020011496	No Hit
GCGTTGATACCACTGCTTCCCATGT	5042	0.24189191912116761	No Hit
CATGTACTCTGCGTTGATACCACTG	4767	0.22869868672165927	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	3968	0.19036634967726956	No Hit
TATCAACGCAGAGTACATGGGAAGC	3924	0.1882554324933482	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3786	0.1816348285983222	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3455	0.16575497432836853	No Hit
GGTATCAACGCAGAGTACATGGGAA	2509	0.12037025487405979	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2091	0.10031654162680712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	9.595078108733344E-5
13	0.0	0.0	0.0	0.0	1.919015621746669E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCGAC	60	2.569823E-5	14.249569	19
CGTGCGC	110	1.8735591E-10	13.816437	10
ATACGAC	105	1.371518E-9	13.571016	19
GCGTTTA	230	0.0	12.802116	13
GTCTAAC	90	5.319289E-7	12.683938	1
TGATACG	90	5.417787E-7	12.6647625	17
AAGGCGT	170	1.8189894E-12	11.734375	6
GATACGA	130	2.6175258E-9	11.692234	18
GTCCTAC	755	0.0	11.465944	1
GTCCTAA	385	0.0	11.366127	1
GGTATCG	85	5.3328047E-5	11.17479	17
TCTATAC	230	0.0	11.153442	3
CGCAGAT	120	1.2777127E-7	11.0822	8
TCCTACA	835	0.0	11.036919	2
TCCAACG	130	3.2679964E-8	10.96147	18
GACTGCG	70	0.0014930452	10.856032	7
TAGGACT	230	0.0	10.74061	4
AAAGGCG	205	0.0	10.658726	5
GTCTTAG	300	0.0	10.46425	1
CGTTTAT	275	0.0	10.361831	14
>>END_MODULE