FastQCFastQC Report
Thu 26 May 2016
SRR1513057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2084402
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC209491.0050364564992742No Hit
CCCATGTACTCTGCGTTGATACCAC206630.9913154948037856No Hit
GAGTACATGGGAAGCAGTGGTATCA77680.3726728337432031No Hit
CATGTACTCTGCGTTGATACCACTG60340.289483506540485No Hit
GCGTTGATACCACTGCTTCCCATGT54440.26117802611972163No Hit
GTATCAACGCAGAGTACATGGGAAG53800.258107601124927No Hit
ACGCAGAGTACATGGGAAGCAGTGG50480.24217977146442962No Hit
GCTTCCCATGTACTCTGCGTTGATA48320.23181708710699758No Hit
TATCAACGCAGAGTACATGGGAAGC45400.2178082730682469No Hit
ACTCTGCGTTGATACCACTGCTTCC42480.20379945902949623No Hit
GTATCAACGCAGAGTACTTTTTTTT33490.16066958293073985No Hit
GGTATCAACGCAGAGTACATGGGAA30350.14560531030002852No Hit
TATCAACGCAGAGTACTTTTTTTTT24550.1177795837847018No Hit
CAGTGGTATCAACGCAGAGTACATG22560.10823248106651212No Hit
ATACCACTGCTTCCCATGTACTCTG21450.10290721271616513No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTT350.00219231816.2613838
AACCGTG508.828071E-515.1772917
GACCGTG751.5020658E-512.6477427
AGAACCG853.8448215E-612.3224695
ACCGTGC1052.7746864E-711.7443318
GTCCTAC6950.011.6578531
CTACACT3850.011.1295014
TCGCACG600.00592665711.07075918
CCAACGA1053.5311386E-610.84066219
TGTGCGC803.83194E-410.66922910
TCCAACG1451.4697434E-810.47086518
AGGGCCG1105.883272E-610.3875355
GTCTTAG2400.010.32634451
TAGGACA3250.010.2543614
CACGGAT951.6694282E-49.9850597
CTAGACT2201.8189894E-129.9547214
CTAGCAC2201.8189894E-129.9547213
TCCTACA9000.09.9520552
TATGGCG6200.09.95124416
GTGCGCC1054.1762378E-59.93703511
Produced by FastQC (version 0.11.5)