FastQCFastQC Report
Thu 26 May 2016
SRR1513056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019401
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC135480.6708920120372328No Hit
CCCATGTACTCTGCGTTGATACCAC109130.5404077743845823No Hit
GAGTACATGGGAAGCAGTGGTATCA54250.2686440186966333No Hit
GTATCAACGCAGAGTACATGGGAAG37150.18396544321806318No Hit
GCGTTGATACCACTGCTTCCCATGT36630.18139042220935814No Hit
CATGTACTCTGCGTTGATACCACTG35740.17698317471368985No Hit
TATCAACGCAGAGTACATGGGAAGC29020.14370598013965527No Hit
ACGCAGAGTACATGGGAAGCAGTGG28800.1426165481744339No Hit
GCTTCCCATGTACTCTGCGTTGATA27140.13439628880049084No Hit
GTATCAACGCAGAGTACTTTTTTTT25420.12587891161785103No Hit
ACTCTGCGTTGATACCACTGCTTCC24010.11889664311347771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTTA2400.013.06037213
GTAGGAC10500.012.9404743
GGACGTG7550.012.4558496
GACGTGG4050.012.4294587
AGGACGT7700.012.3393195
CGTTTAT2550.012.29211514
TGCGTTT2650.012.18608912
TAGGACG8550.012.114244
TAGGACC5100.012.11097054
AGACCCG1052.7164788E-711.7634855
GTCTAGA1701.8189894E-1211.7499711
CGGTGTA658.0395676E-411.6898239
ACGTGGA4250.011.6210598
TATCGAC907.466788E-611.61065719
GACGTGA3700.011.5515997
GTGTAGA4100.011.3678581
TGTAGGA12800.011.2838842
GTCCTAC10900.011.082651
GTGTAGG3000.010.7801331
AGGACCT8400.010.7454915
Produced by FastQC (version 0.11.5)