##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513056_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2019401
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.339255551522456	31.0	30.0	34.0	25.0	34.0
2	29.059453768716565	31.0	30.0	34.0	16.0	34.0
3	29.3981675754345	31.0	30.0	34.0	23.0	34.0
4	32.24329442245497	35.0	33.0	37.0	19.0	37.0
5	32.49014237390196	35.0	33.0	37.0	22.0	37.0
6	32.9200817470131	37.0	33.0	37.0	26.0	37.0
7	32.92196101715311	37.0	35.0	37.0	26.0	37.0
8	32.90018376736468	37.0	35.0	37.0	26.0	37.0
9	34.27043415349403	39.0	35.0	39.0	26.0	39.0
10	33.768020318896546	39.0	34.0	39.0	19.0	39.0
11	34.15265962530473	39.0	35.0	39.0	25.0	39.0
12	33.98103497027089	39.0	34.0	39.0	24.0	39.0
13	34.07142761640704	39.0	35.0	39.0	25.0	39.0
14	34.37845133284573	40.0	34.0	41.0	17.0	41.0
15	34.92489753149572	40.0	34.0	41.0	23.0	41.0
16	34.50923070752169	39.0	34.0	41.0	17.0	41.0
17	34.05295877341845	39.0	33.0	41.0	15.0	41.0
18	33.4665031858457	39.0	32.0	41.0	10.0	41.0
19	34.35351770153625	39.0	34.0	41.0	18.0	41.0
20	34.62189877097219	39.0	34.0	41.0	18.0	41.0
21	34.639918966069644	40.0	34.0	41.0	18.0	41.0
22	34.2362893749186	40.0	34.0	41.0	10.0	41.0
23	32.56688790388833	39.0	31.0	41.0	2.0	41.0
24	33.65968621388224	39.0	33.0	41.0	2.0	41.0
25	33.40327354497695	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7798222448270984
1101	2	1.0951816532333893
1101	3	0.9020963465563199
1101	4	0.860058628422685
1101	5	1.024364648842898
1101	6	0.7315476484081245
1101	7	0.7065936930644199
1101	8	0.6457702845336541
1101	9	0.8845805214709657
1101	10	1.1817365360611163
1101	11	0.804205759456444
1101	12	0.889713907860326
1101	13	0.7989907048747398
1101	14	-0.22439432687720284
1101	15	0.27259112644718897
1101	16	1.0049665064134032
1101	17	1.6135991237785277
1101	18	-4.480588629475559
1101	19	-0.882080876884622
1101	20	0.17268054208503258
1101	21	0.5319676584487922
1101	22	1.1110742799348117
1101	23	3.067765761024127
1101	24	1.7886987876157363
1101	25	2.115228253537012
1102	1	1.0168077659791592
1102	2	1.369020151739761
1102	3	1.0766750486200642
1102	4	1.6429938376003363
1102	5	1.3461549942321724
1102	6	0.9278918717912816
1102	7	0.9540213894744198
1102	8	0.9007670350908015
1102	9	1.0747025499042877
1102	10	1.5072223765790156
1102	11	1.182328223008298
1102	12	1.2087308746936927
1102	13	1.020232521996654
1102	14	1.8988150627574925
1102	15	1.3407169649568544
1102	16	1.6454414550219951
1102	17	1.478439371126676
1102	18	1.8495901317196797
1102	19	1.534704545529003
1102	20	1.471096766427685
1102	21	1.3791039370139089
1102	22	1.771024910717216
1102	23	3.421025551888313
1102	24	2.159474442176247
1102	25	2.4966858448212577
1103	1	0.8109278996876412
1103	2	1.107072780853045
1103	3	0.8899074484007095
1103	4	1.3648916602905459
1103	5	1.2386025593985224
1103	6	0.9104668899102748
1103	7	0.8377926741175088
1103	8	0.8035924948170461
1103	9	0.9131507378677739
1103	10	1.278604352389138
1103	11	0.96094368436043
1103	12	1.1029513724353635
1103	13	0.959588505671654
1103	14	1.7321584086110704
1103	15	1.3547308924643673
1103	16	1.60300306646824
1103	17	1.9598925276885026
1103	18	1.9331754590601946
1103	19	1.5181489443506493
1103	20	1.394633702982297
1103	21	1.3246358636340716
1103	22	1.620806683704906
1103	23	3.1157709628930803
1103	24	2.0992510619310423
1103	25	2.2781574648378466
1104	1	0.6755801131356449
1104	2	1.0314594898197775
1104	3	0.858750783034619
1104	4	1.4140535580854703
1104	5	1.1875965476133032
1104	6	0.6934712697634495
1104	7	0.6839227227423521
1104	8	0.7746087704917528
1104	9	0.9524061573300457
1104	10	1.2374336885113948
1104	11	0.9990805861323864
1104	12	1.069339333165658
1104	13	0.8072543997981398
1104	14	1.7020363776054594
1104	15	1.162850139159083
1104	16	1.452083543563198
1104	17	2.0120437723005367
1104	18	2.6004412923685223
1104	19	1.7725903907631135
1104	20	1.145302323481907
1104	21	1.2460647782288632
1104	22	1.638660860176742
1104	23	3.1077493858579004
1104	24	2.075435782038319
1104	25	2.039851191299256
1105	1	0.6838755172720852
1105	2	1.1033067234070089
1105	3	0.7532019736829874
1105	4	0.898635489195243
1105	5	0.850037534506896
1105	6	0.5778785437567251
1105	7	0.44475183605819524
1105	8	0.5126243899760823
1105	9	0.723148793047649
1105	10	1.2523724048301261
1105	11	0.7125341992665497
1105	12	0.7559673215964793
1105	13	0.770363179990639
1105	14	1.4590639969202428
1105	15	0.7830197681696376
1105	16	1.2441270350618439
1105	17	1.6507287381666913
1105	18	1.6243125003790624
1105	19	1.2187186359379538
1105	20	0.954401253893117
1105	21	0.7945337458525827
1105	22	1.3150744163904164
1105	23	2.6445563185612
1105	24	1.7883403156491369
1105	25	1.7033204485082294
1106	1	-0.47612611831836205
1106	2	0.023297066048023396
1106	3	-0.2554006030010392
1106	4	-2.51771642392497
1106	5	-0.39894737988274187
1106	6	-0.37984274468941237
1106	7	-0.3529711790855501
1106	8	-0.1600858267808718
1106	9	-0.26140800098682604
1106	10	-0.16998162631463742
1106	11	-0.21218807573998077
1106	12	-0.10360454850379597
1106	13	-0.15944818914173453
1106	14	0.5401225796919675
1106	15	-0.0754704213245816
1106	16	-1.0357135968227098
1106	17	-5.778473731444734
1106	18	-6.721032552805827
1106	19	-2.7542779609926846
1106	20	-1.3733076222870508
1106	21	-1.0185846439975563
1106	22	-1.2400766522965725
1106	23	-5.341843334710983
1106	24	-2.239149426118466
1106	25	-0.7027415616280948
1107	1	0.42058524822058985
1107	2	0.6933776240596146
1107	3	0.4119169134544052
1107	4	0.789018029361209
1107	5	0.8680606759184215
1107	6	0.5049691228810715
1107	7	0.39130625484705917
1107	8	0.35805200601213727
1107	9	0.26751183396748957
1107	10	0.8819512747561902
1107	11	0.4900174454310644
1107	12	0.524907584318683
1107	13	0.4749338719644669
1107	14	1.2826439472133373
1107	15	0.7452388821433473
1107	16	-5.837462295123132
1107	17	-1.0593092752113122
1107	18	0.6708895417024721
1107	19	0.5943442022846526
1107	20	0.5400065539581647
1107	21	0.5755306914970859
1107	22	0.7920483108628673
1107	23	2.4097776496686265
1107	24	1.4742433433087854
1107	25	1.2676386328900335
1108	1	0.5465318861731348
1108	2	0.6981997772007738
1108	3	0.5942915319413338
1108	4	1.103108537844534
1108	5	0.8728026237339463
1108	6	0.5283174770432097
1108	7	0.42662118213370803
1108	8	0.4361096112593472
1108	9	0.6192527504838878
1108	10	1.0092773828496178
1108	11	0.5021594869116583
1108	12	0.657108393870061
1108	13	0.49887531531614826
1108	14	1.051031248589915
1108	15	0.7833201249141126
1108	16	1.0853749575156542
1108	17	1.211629846180827
1108	18	0.997268366513147
1108	19	0.8663822288280016
1108	20	0.7264553571472021
1108	21	0.8202847715204911
1108	22	1.2906617728197318
1108	23	2.9334041104196373
1108	24	1.673357520045677
1108	25	1.7812016300247677
1109	1	0.15809193635423924
1109	2	0.48415047596099114
1109	3	0.34528280450054893
1109	4	0.3522881796219579
1109	5	0.17499161215970105
1109	6	0.18032670968067066
1109	7	0.13932336023093228
1109	8	-0.06693798715967603
1109	9	0.09783033121389195
1109	10	0.6418949767061619
1109	11	0.2541019038728862
1109	12	0.33497372275472515
1109	13	0.13829990915495216
1109	14	-0.9707971432183484
1109	15	-0.06912410306603789
1109	16	-0.3230900135094714
1109	17	0.7501367646422494
1109	18	1.5284331069262933
1109	19	0.7364464399981756
1109	20	0.4596028058428132
1109	21	0.4373747571029085
1109	22	0.24433705312026177
1109	23	-4.178631155041121
1109	24	-1.405115513322798
1109	25	-0.09093017552203975
1110	1	0.8196968243250282
1110	2	1.1628429295060876
1110	3	0.8953523223778213
1110	4	1.505611364032191
1110	5	1.1310629722210876
1110	6	0.8184554422040975
1110	7	0.6438868085713025
1110	8	0.661102250770611
1110	9	0.7134468049691094
1110	10	1.0783241675561186
1110	11	0.7886801118811562
1110	12	0.8288702988605507
1110	13	0.7396775045815147
1110	14	1.544784333794773
1110	15	0.9995376817598824
1110	16	1.4480372382911
1110	17	1.6957964948462987
1110	18	2.428754617200191
1110	19	1.5473651460937106
1110	20	1.1194905546843188
1110	21	1.0394483239803733
1110	22	1.4005366685128067
1110	23	2.8285033450088193
1110	24	1.905861058280614
1110	25	2.1362179031052975
1111	1	0.6076109870270585
1111	2	0.8738287308670181
1111	3	0.6742919691970002
1111	4	1.1539581627122644
1111	5	0.9250096466416267
1111	6	0.4182952261453039
1111	7	0.35858490234905105
1111	8	0.2304397892314327
1111	9	0.4186794641776004
1111	10	0.6677873258529416
1111	11	0.37561228130130786
1111	12	0.4795666472362541
1111	13	0.32941116511602075
1111	14	-0.4871015072450149
1111	15	0.11854619361560026
1111	16	0.6052300253138512
1111	17	1.1294027145268615
1111	18	1.6907785196198262
1111	19	0.8695104120317083
1111	20	0.7147115324056941
1111	21	0.5832164825149562
1111	22	0.9751485133926323
1111	23	0.8131406498398945
1111	24	0.7443943164138105
1111	25	0.366690779214494
1112	1	0.6932149669529544
1112	2	1.009473736210257
1112	3	0.7879595639637778
1112	4	1.3538785835802258
1112	5	1.1378777183016169
1112	6	0.529460197757075
1112	7	0.5610814189445961
1112	8	0.5060537286650231
1112	9	0.6458269012400564
1112	10	0.9792449276713384
1112	11	0.744885721887151
1112	12	0.7838119176817173
1112	13	0.6710647341819325
1112	14	1.0192045816248125
1112	15	0.8093209754636135
1112	16	1.1541659088627299
1112	17	1.37907618322226
1112	18	1.4589821491455268
1112	19	0.9290283330874658
1112	20	0.8554801349971015
1112	21	0.8793537928662971
1112	22	0.93148874568422
1112	23	2.541594454444528
1112	24	1.5888600530417776
1112	25	1.5973098437962037
1113	1	0.26953229303772375
1113	2	0.6445809545732892
1113	3	0.41774311374732775
1113	4	0.6026171318536555
1113	5	0.6312795103080617
1113	6	0.1948301766154259
1113	7	0.011185113699454519
1113	8	0.0036637155773320274
1113	9	0.15003710963631534
1113	10	0.5120185106774784
1113	11	0.11807408391271679
1113	12	0.10832616312619336
1113	13	0.008026377190091694
1113	14	0.7112090113036587
1113	15	0.23703063015000936
1113	16	0.514363230920516
1113	17	1.0285275070921926
1113	18	1.570702676680007
1113	19	0.6359285646388599
1113	20	0.3467959509640792
1113	21	0.3854003044942047
1113	22	0.6839059408921102
1113	23	0.697390286519429
1113	24	0.5828236088657945
1113	25	0.9805374688842932
1114	1	-0.5350135097529218
1114	2	-3.2638020670172807
1114	3	-1.2116144625167884
1114	4	-0.06784857327637894
1114	5	0.1996650519274823
1114	6	-0.0013262095365718096
1114	7	-0.05884572816113831
1114	8	-0.10478616909018967
1114	9	0.06637531776916461
1114	10	0.10259170326909839
1114	11	-0.010397366629817384
1114	12	0.004283163076173935
1114	13	-0.1147731089266415
1114	14	0.6364407684792539
1114	15	0.013088709850038072
1114	16	0.5220239919818894
1114	17	0.8334316858445447
1114	18	0.9703640557183064
1114	19	0.3256389322781459
1114	20	0.03607702725700079
1114	21	0.029619086053926935
1114	22	0.18640032622985103
1114	23	1.8895671798810554
1114	24	0.7484521882723527
1114	25	0.6280139259263535
1115	1	-0.0646109220952873
1115	2	0.24247022965347753
1115	3	0.012238478189235735
1115	4	0.13824258842032577
1115	5	0.11207312020609095
1115	6	-0.2660870927244403
1115	7	-0.45908606317379963
1115	8	-0.466311676052932
1115	9	-0.42310967194130455
1115	10	-0.152336087972067
1115	11	-0.21541410816035977
1115	12	-0.371895215061933
1115	13	-0.5078879650433308
1115	14	0.47634421029120233
1115	15	-0.1642664402749503
1115	16	0.3028088736759287
1115	17	0.6739860718038813
1115	18	1.1604742999938011
1115	19	0.3435282312989614
1115	20	-0.1330996980697492
1115	21	-0.10277964843147203
1115	22	0.2549619066861837
1115	23	1.9130347503402803
1115	24	0.6497485180025109
1115	25	0.36453257305479525
1116	1	-0.060617177465541516
1116	2	-0.11334255245613178
1116	3	0.011399206542922258
1116	4	0.6307765288368259
1116	5	0.13027676716975378
1116	6	0.006138486999262227
1116	7	-0.2095840534757798
1116	8	-0.19115834601802106
1116	9	-0.10192141135390642
1116	10	0.2920867007005654
1116	11	-0.16010473480542942
1116	12	-0.056003400659562885
1116	13	-0.14796600699717288
1116	14	0.3753574024985298
1116	15	-0.12705299423239325
1116	16	0.23446233785305992
1116	17	0.44651057964805574
1116	18	0.9949910206894046
1116	19	0.14232967768083427
1116	20	-0.015150576325844156
1116	21	-0.1407632859219703
1116	22	0.21721814226995662
1116	23	1.5754398208755447
1116	24	0.5291289717157355
1116	25	0.6590139906031993
1201	1	0.8863948197050888
1201	2	1.2658735648106223
1201	3	0.9724276407819765
1201	4	1.6054324770787218
1201	5	1.3513606367699325
1201	6	0.9647389169343583
1201	7	0.7738023881316138
1201	8	0.8914504798628684
1201	9	0.9677091808551879
1201	10	1.380034924165436
1201	11	0.9313903379909263
1201	12	1.2150533963931025
1201	13	1.0355803354440596
1201	14	1.894844798211622
1201	15	1.3860604869746567
1201	16	1.6820487338403325
1201	17	2.15344414707252
1201	18	2.6463546706678613
1201	19	1.8457468091889027
1201	20	1.5845549665190504
1201	21	1.5571739011597074
1201	22	1.8032864941162998
1201	23	3.3084068107999514
1201	24	2.2105452230780074
1201	25	2.2038541934514626
1202	1	1.2198232275387326
1202	2	1.388147351276217
1202	3	1.1802656679973325
1202	4	1.725706777126426
1202	5	1.501469562253888
1202	6	1.163882235000699
1202	7	1.0209958641778414
1202	8	1.026399068270635
1202	9	1.157589927538396
1202	10	1.6707513936873681
1202	11	1.3437993583808066
1202	12	1.3307570404098996
1202	13	1.2870203601229733
1202	14	2.10698803704922
1202	15	1.6387999755390226
1202	16	1.8671791269441727
1202	17	2.266154292903991
1202	18	2.855855265445399
1202	19	2.0832477433576813
1202	20	1.900227930466599
1202	21	1.687320215870912
1202	22	2.0714378288959807
1202	23	3.677622788935061
1202	24	2.4465898091976896
1202	25	2.6388150876012872
1203	1	0.8329270378278189
1203	2	1.071400646509261
1203	3	0.7558886891022034
1203	4	1.4389505658615107
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2211	13	-0.4881438911394653
2211	14	-0.1160993620446007
2211	15	-0.29198266571457054
2211	16	-0.5091237072855037
2211	17	0.0381373560719922
2211	18	-5.508739059571024
2211	19	-1.7815084928192277
2211	20	-0.8125609942074661
2211	21	-0.7865659605313269
2211	22	-0.4323613435026772
2211	23	-4.817825050478156
2211	24	-2.190688466791805
2211	25	-1.4981352624478355
2212	1	0.28728489200381446
2212	2	0.5675931941322929
2212	3	0.2548945053938496
2212	4	0.7885737071578021
2212	5	0.6590993092795969
2212	6	0.2815201958657667
2212	7	0.15733730806545054
2212	8	0.11605719606299658
2212	9	-0.02035112903541858
2212	10	-3.901844077767386
2212	11	-1.0897977628720596
2212	12	-0.24799503311760418
2212	13	-0.050363930772853394
2212	14	0.8669603022252801
2212	15	0.4382750579003911
2212	16	0.6591190504950291
2212	17	1.166451944898057
2212	18	1.7109066035152907
2212	19	0.9725891325772977
2212	20	0.5447931294953321
2212	21	0.48422842096967855
2212	22	0.762612788815737
2212	23	1.9976342010092623
2212	24	1.1810199510081318
2212	25	1.1175931520333364
2213	1	-1.4724315370375756
2213	2	-3.7447495425158266
2213	3	-1.8468178064872092
2213	4	-3.3667359388927593
2213	5	-1.409249577684939
2213	6	-1.1832720739808131
2213	7	-1.0287243052697121
2213	8	-0.9132830605815485
2213	9	-0.7712128513089169
2213	10	-0.934973100357837
2213	11	-0.9364444228411557
2213	12	-0.8114247455481305
2213	13	-1.0751596328704238
2213	14	-6.220913797723718
2213	15	-2.836269966606885
2213	16	-2.1238151504415015
2213	17	-0.7856023786595898
2213	18	0.019423112218802885
2213	19	-0.7635854638346729
2213	20	-0.9124086075087234
2213	21	-1.269958270371184
2213	22	-1.054660775070353
2213	23	-5.6820053278902165
2213	24	-3.1788993313856118
2213	25	-2.1952102997864884
2214	1	-0.36640729191249477
2214	2	-0.15175870820891646
2214	3	-0.3864660534430193
2214	4	-0.08522435230044323
2214	5	-0.3119371070428585
2214	6	-0.4565988231106104
2214	7	-0.6567933370304644
2214	8	-0.7147429772351686
2214	9	-0.6365774315057209
2214	10	-0.13063159096932964
2214	11	-0.6499284400885514
2214	12	-0.5039186029680067
2214	13	-0.6456359538262575
2214	14	-0.1150506447734756
2214	15	-0.40905803554831266
2214	16	0.0589777964312006
2214	17	0.2848754478305864
2214	18	0.7748994933960134
2214	19	-0.3721789033182148
2214	20	-0.4023840070775364
2214	21	-0.5233035676841098
2214	22	0.11264850383891911
2214	23	1.4732431153872696
2214	24	0.42851301640486383
2214	25	0.29985907523609256
2215	1	-2.47561352312211
2215	2	-4.323955781098771
2215	3	-2.5131796575727137
2215	4	-4.00365278520222
2215	5	-5.003256326193686
2215	6	-2.414012779450321
2215	7	-1.8431520383652504
2215	8	-2.169465405951673
2215	9	-1.8755687200730051
2215	10	-3.600667062628524
2215	11	-2.251083542836767
2215	12	-1.9316578988057955
2215	13	-1.818024242807482
2215	14	-1.14500353543081
2215	15	-1.813971895205725
2215	16	-2.5041564573175705
2215	17	-6.97164792135397
2215	18	-3.3805658134162933
2215	19	-2.4501547843087543
2215	20	-2.114189488032558
2215	21	-2.1817309334998285
2215	22	-1.8213522237848636
2215	23	-6.806889353677974
2215	24	-3.8841664724823985
2215	25	-3.4566104025219637
2216	1	-1.4760206330862928
2216	2	-1.0467811291938567
2216	3	-1.2706202286637058
2216	4	-3.6356703648383153
2216	5	-1.8694961082773887
2216	6	-1.5302225288317466
2216	7	-1.6166152415074393
2216	8	-1.482290234963017
2216	9	-1.6169369753831049
2216	10	-1.1927290362837866
2216	11	-1.7501170583437755
2216	12	-2.4504994027644287
2216	13	-1.8877211226198227
2216	14	-7.567140232426802
2216	15	-4.0231769802489445
2216	16	-2.3796959954319092
2216	17	-1.5326105992328465
2216	18	-0.7663827152773308
2216	19	-1.3492224110331676
2216	20	-1.7506088978096201
2216	21	-2.0182630586039494
2216	22	-1.437269308808304
2216	23	-0.2093648887667996
2216	24	-0.9987289961192474
2216	25	-0.836968768050852
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	94900.0
3	1.0
4	765.0
5	4850.0
6	6170.0
7	5270.0
8	5025.0
9	4671.0
10	4499.0
11	4373.0
12	4368.0
13	4333.0
14	4259.0
15	4356.0
16	4448.0
17	4803.0
18	5322.0
19	6173.0
20	7679.0
21	9947.0
22	11973.0
23	14498.0
24	17415.0
25	20261.0
26	22767.0
27	26398.0
28	29179.0
29	35602.0
30	42309.0
31	48052.0
32	61976.0
33	80900.0
34	106693.0
35	138930.0
36	197106.0
37	277512.0
38	701618.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01868945755956	17.60898878632546	11.881339295452067	25.490982460662902
2	14.041818492582308	19.268312620604068	41.4753639487041	25.21450493810952
3	19.809026178155708	24.015235502346552	28.334091244589356	27.841647074908384
4	11.005244720605194	15.876771851681601	38.269370990176306	34.8486124375369
5	12.587617661540962	37.72109372600577	36.0281842796949	13.66310433275838
6	33.59499324676714	35.19615578348645	17.741512892557974	13.467338077188437
7	29.297943746743453	30.786939354496162	21.05579362579321	18.859323272967174
8	26.493884982595034	35.964187573428745	19.760382057332375	17.78154538664384
9	26.998763651349684	15.184748926120243	19.19465952860245	38.62182789392762
10	14.486344035686068	29.130957069224728	34.2443088796586	22.13839001543061
11	34.973202120056285	22.754300018375407	22.663314517115627	19.60918334445268
12	23.7893631563828	24.897177838753453	30.677259264395595	20.636199740468147
13	29.354808354258598	20.939334056431363	24.82672887290809	24.879128716401944
14	21.07124690377827	21.60443349729296	27.693904832755734	29.630414766173036
15	25.058457070147988	28.758798278160064	22.5771333673105	23.60561128438145
16	22.511185086749123	27.57170317927392	27.68804888401735	22.229062849959607
17	22.789401550052776	27.72090134276157	27.652200531081544	21.837496576104105
18	22.421801403250875	26.557657008874575	29.346278849306547	21.674262738568004
19	23.645397062065175	27.156008747922616	27.563980948748366	21.634613241263843
20	23.862964331458787	26.708611980913116	27.490393654611395	21.9380300330167
21	23.076496328093036	26.49667482645992	27.508591558923385	22.91823728652366
22	23.205559240558124	27.085297269074594	26.358193147744608	23.350950342622678
23	21.250497186284743	26.86985384333047	28.87553922731679	23.004109743067996
24	22.918665762418996	27.699503770148038	26.96365489113608	22.418175576296886
25	22.934950002501484	26.352523106537003	26.818096484002137	23.894430406959376
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	172.0
1	172.0
2	324.5
3	477.0
4	477.0
5	477.0
6	974.0
7	1471.0
8	1471.0
9	1471.0
10	2252.0
11	3033.0
12	3033.0
13	3033.0
14	5164.0
15	7295.0
16	7295.0
17	7295.0
18	13097.5
19	18900.0
20	18900.0
21	18900.0
22	29885.5
23	40871.0
24	40871.0
25	40871.0
26	58342.5
27	75814.0
28	75814.0
29	75814.0
30	98329.0
31	120844.0
32	120844.0
33	120844.0
34	142654.0
35	164464.0
36	164464.0
37	164464.0
38	185507.5
39	206551.0
40	206551.0
41	206551.0
42	227491.5
43	248432.0
44	248432.0
45	248432.0
46	279013.0
47	309594.0
48	309594.0
49	309594.0
50	294149.5
51	278705.0
52	278705.0
53	278705.0
54	244150.5
55	209596.0
56	209596.0
57	209596.0
58	178728.5
59	147861.0
60	147861.0
61	147861.0
62	120769.5
63	93678.0
64	93678.0
65	93678.0
66	72790.5
67	51903.0
68	51903.0
69	51903.0
70	38022.0
71	24141.0
72	24141.0
73	24141.0
74	16904.5
75	9668.0
76	9668.0
77	9668.0
78	6789.5
79	3911.0
80	3911.0
81	3911.0
82	2657.0
83	1403.0
84	1403.0
85	1403.0
86	922.0
87	441.0
88	441.0
89	441.0
90	291.0
91	141.0
92	141.0
93	141.0
94	80.5
95	20.0
96	20.0
97	20.0
98	17.5
99	15.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.10998310885257559
2	0.04535998546103523
3	0.034416146174038736
4	0.030157457582718837
5	0.03401998909577642
6	0.018223225600066555
7	0.019263137930505134
8	0.021640080400079035
9	0.02738435803488262
10	0.033970469460993635
11	0.019708814643550242
12	0.01886698085224282
13	0.015400606417447551
14	0.020204010991378137
15	0.019708814643550242
16	0.020699207339206032
17	0.024858856660960355
18	0.018569863043546082
19	0.031494487721854154
20	0.02094680551311998
21	0.027681475843579356
22	0.035059901426215
23	0.026740602782706353
24	0.03862531513057585
25	0.02971178086967373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2019401.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.31861935428581
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.067367077846	63.96407892684027
2	9.686767256075472	14.398143369556655
3	2.318548897246099	5.169340588461958
4	0.780664608679502	2.320716635832576
5	0.3597980910255704	1.3369848685664023
6	0.21945851349688023	0.9785912237179222
7	0.13168245733542666	0.6850520895643988
8	0.08614564252883275	0.5121780172904558
9	0.05465313301203892	0.3655570849956785
>10	0.26617513469442783	3.3975053600040597
>50	0.015675387926118636	0.8100985178129705
>100	0.009613132248820964	1.3866400774859802
>500	0.0015561835559246787	0.8769545776598242
>1k	0.0016915038651355082	2.2953682661132717
>5k	6.766015460542033E-5	0.27279120319980066
>10k+	1.3532030921084066E-4	1.2299991928977554
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	13548	0.6708920120372328	No Hit
CCCATGTACTCTGCGTTGATACCAC	10913	0.5404077743845823	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5425	0.2686440186966333	No Hit
GTATCAACGCAGAGTACATGGGAAG	3715	0.18396544321806318	No Hit
GCGTTGATACCACTGCTTCCCATGT	3663	0.18139042220935814	No Hit
CATGTACTCTGCGTTGATACCACTG	3574	0.17698317471368985	No Hit
TATCAACGCAGAGTACATGGGAAGC	2902	0.14370598013965527	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2880	0.1426165481744339	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2714	0.13439628880049084	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2542	0.12587891161785103	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2401	0.11889664311347771	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	4.951963478278955E-5
10	0.0	0.0	0.0	0.0	4.951963478278955E-5
11	0.0	0.0	0.0	0.0	4.951963478278955E-5
12	0.0	0.0	0.0	0.0	1.4855890434836865E-4
13	0.0	0.0	0.0	0.0	1.4855890434836865E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTTA	240	0.0	13.060372	13
GTAGGAC	1050	0.0	12.940474	3
GGACGTG	755	0.0	12.455849	6
GACGTGG	405	0.0	12.429458	7
AGGACGT	770	0.0	12.339319	5
CGTTTAT	255	0.0	12.292115	14
TGCGTTT	265	0.0	12.186089	12
TAGGACG	855	0.0	12.11424	4
TAGGACC	510	0.0	12.1109705	4
AGACCCG	105	2.7164788E-7	11.763485	5
GTCTAGA	170	1.8189894E-12	11.749971	1
CGGTGTA	65	8.0395676E-4	11.689823	9
ACGTGGA	425	0.0	11.621059	8
TATCGAC	90	7.466788E-6	11.610657	19
GACGTGA	370	0.0	11.551599	7
GTGTAGA	410	0.0	11.367858	1
TGTAGGA	1280	0.0	11.283884	2
GTCCTAC	1090	0.0	11.08265	1
GTGTAGG	300	0.0	10.780133	1
AGGACCT	840	0.0	10.745491	5
>>END_MODULE